UniProtKB - P01096 (ATIF1_BOVIN)
ATPase inhibitor, mitochondrial
ATP5IF1
Functioni
Endogenous F1F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F1F(o)-ATP synthase enzyme acts as an ATP hydrolase (PubMed:10831597, PubMed:12923572, PubMed:17895376, PubMed:18687699, PubMed:21192948, PubMed:7397110).
Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme (By similarity).
By similarity6 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 51 | Participates in pH sensing | 1 | |
Sitei | 74 | Participates in pH sensing | 1 |
GO - Molecular functioni
- angiostatin binding Source: UniProtKB
- ATPase binding Source: UniProtKB
- ATPase inhibitor activity Source: UniProtKB
- calmodulin binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- structural molecule activity Source: UniProtKB
GO - Biological processi
- erythrocyte differentiation Source: UniProtKB
- heme biosynthetic process Source: UniProtKB
- negative regulation of ATP-dependent activity Source: GO_Central
- negative regulation of endothelial cell proliferation Source: UniProtKB
- negative regulation of hydrolase activity Source: UniProtKB
- protein homotetramerization Source: UniProtKB
Names & Taxonomyi
Protein namesi | Recommended name: ATPase inhibitor, mitochondrialCuratedAlternative name(s): ATP synthase F1 subunit epsilonBy similarity Inhibitor of F(1)F(o)-ATPase Short name: IF(1) Short name: IF1 |
Gene namesi | |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000006342 |
VGNCi | VGNC:103019, ATP5IF1 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrial proton-transporting ATP synthase complex Source: CAFA
- mitochondrion Source: UniProtKB
Other locations
- cell surface Source: UniProtKB
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 46 | A → V: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 47 | F → A: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 49 | K → A: No effect on F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 50 | R → A: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 51 | E → A: No effect on F(1)F(o)-ATP synthase-binding. 2 Publications | 1 | |
Mutagenesisi | 51 | E → A: Retains its inhibitory activity at pH 8.2 although it still form a homotetramer at high pH. 2 Publications | 1 | |
Mutagenesisi | 52 | Q → A: Impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 53 | A → V: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 54 | E → A: No effect on F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 55 | E → A: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 56 | E → A: No effect on F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 57 | R → A: No effect on F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 58 | Y → A: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 59 | F → A: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 60 | R → A: No effect on F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 62 | R → A: Impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 64 | K → A: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 65 | E → A: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 66 | Q → A: Impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 67 | L → A: Impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 68 | A → V: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 69 | A → V: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 70 | L → A: Strongly impairs F(1)F(o)-ATP synthase-binding. 1 Publication | 1 | |
Mutagenesisi | 74 | H → K: Retains its inhibitory activity at pH 8.2 and does not form a homotetramer at high pH. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 25 | Mitochondrion1 PublicationAdd BLAST | 25 | |
ChainiPRO_0000002546 | 26 – 109 | ATPase inhibitor, mitochondrialAdd BLAST | 84 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 103 | N6-succinyllysineBy similarity | 1 |
Proteomic databases
PaxDbi | P01096 |
PRIDEi | P01096 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000006342, Expressed in corpus luteum and 95 other tissues |
Interactioni
Subunit structurei
Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0.
6 PublicationsGO - Molecular functioni
- angiostatin binding Source: UniProtKB
- ATPase binding Source: UniProtKB
- calmodulin binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
CORUMi | P01096 |
DIPi | DIP-46311N |
IntActi | P01096, 5 interactors |
STRINGi | 9913.ENSBTAP00000008319 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P01096 |
BMRBi | P01096 |
SMRi | P01096 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P01096 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 26 – 52 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 26 – 52 | N-terminal inhibitory regionAdd BLAST | 27 | |
Regioni | 74 – 106 | Antiparallel alpha-helical coiled coil regionAdd BLAST | 33 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 69 – 109 | Add BLAST | 41 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transit peptidePhylogenomic databases
eggNOGi | ENOG502S4JP, Eukaryota |
GeneTreei | ENSGT00390000006264 |
HOGENOMi | CLU_147479_0_0_1 |
InParanoidi | P01096 |
OMAi | QEVDHHK |
OrthoDBi | 1566610at2759 |
TreeFami | TF320659 |
Family and domain databases
DisProti | DP00844 |
InterProi | View protein in InterPro IPR007648, ATPase_inhibitor_mt |
Pfami | View protein in Pfam PF04568, IATP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAATALAART RQAVWSVWAM QGRGFGSESG DNVRSSAGAV RDAGGAFGKR
60 70 80 90 100
EQAEEERYFR ARAKEQLAAL KKHHENEISH HAKEIERLQK EIERHKQSIK
KLKQSEDDD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 55 | E → Q AA sequence (PubMed:6461003).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22559 mRNA Translation: AAA30396.1 BC111645 mRNA Translation: AAI11646.1 |
PIRi | C27382, IWBO |
RefSeqi | NP_787010.1, NM_175816.3 |
Genome annotation databases
Ensembli | ENSBTAT00000008319; ENSBTAP00000008319; ENSBTAG00000006342 |
GeneIDi | 327699 |
KEGGi | bta:327699 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22559 mRNA Translation: AAA30396.1 BC111645 mRNA Translation: AAI11646.1 |
PIRi | C27382, IWBO |
RefSeqi | NP_787010.1, NM_175816.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GMJ | X-ray | 2.20 | A/B/C/D | 26-109 | [»] | |
1HF9 | NMR | - | A/B | 69-109 | [»] | |
1OHH | X-ray | 2.80 | H | 26-109 | [»] | |
2V7Q | X-ray | 2.10 | J | 26-85 | [»] | |
4TSF | X-ray | 3.20 | H/I | 26-85 | [»] | |
4TT3 | X-ray | 3.21 | H/I/J | 26-85 | [»] | |
4Z1M | X-ray | 3.30 | H/I/J | 26-85 | [»] | |
5LQX | electron microscopy | 7.90 | J | 26-85 | [»] | |
5LQY | electron microscopy | 7.80 | J | 26-85 | [»] | |
5LQZ | electron microscopy | 7.00 | J | 26-85 | [»] | |
6YY0 | electron microscopy | 3.23 | J | 26-85 | [»] | |
6Z1R | electron microscopy | 3.29 | J | 26-85 | [»] | |
6Z1U | electron microscopy | 3.47 | J | 26-85 | [»] | |
6ZPO | electron microscopy | 4.00 | J | 26-85 | [»] | |
6ZQM | electron microscopy | 3.29 | J | 26-85 | [»] | |
6ZQN | electron microscopy | 4.00 | J | 26-85 | [»] | |
7AJB | electron microscopy | 9.20 | AJ/J | 26-85 | [»] | |
7AJC | electron microscopy | 11.90 | AJ/J | 26-85 | [»] | |
7AJD | electron microscopy | 9.00 | AJ/J | 26-85 | [»] | |
7AJE | electron microscopy | 9.40 | AJ/J | 26-85 | [»] | |
7AJF | electron microscopy | 8.45 | AJ/J | 26-109 | [»] | |
7AJG | electron microscopy | 10.70 | AJ/J | 26-85 | [»] | |
7AJH | electron microscopy | 9.70 | AJ/J | 26-85 | [»] | |
7AJI | electron microscopy | 11.40 | AJ/J | 26-85 | [»] | |
7AJJ | electron microscopy | 13.10 | AJ/J | 26-85 | [»] | |
AlphaFoldDBi | P01096 | |||||
BMRBi | P01096 | |||||
SMRi | P01096 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
CORUMi | P01096 |
DIPi | DIP-46311N |
IntActi | P01096, 5 interactors |
STRINGi | 9913.ENSBTAP00000008319 |
Proteomic databases
PaxDbi | P01096 |
PRIDEi | P01096 |
Genome annotation databases
Ensembli | ENSBTAT00000008319; ENSBTAP00000008319; ENSBTAG00000006342 |
GeneIDi | 327699 |
KEGGi | bta:327699 |
Organism-specific databases
CTDi | 93974 |
VEuPathDBi | HostDB:ENSBTAG00000006342 |
VGNCi | VGNC:103019, ATP5IF1 |
Phylogenomic databases
eggNOGi | ENOG502S4JP, Eukaryota |
GeneTreei | ENSGT00390000006264 |
HOGENOMi | CLU_147479_0_0_1 |
InParanoidi | P01096 |
OMAi | QEVDHHK |
OrthoDBi | 1566610at2759 |
TreeFami | TF320659 |
Miscellaneous databases
EvolutionaryTracei | P01096 |
Gene expression databases
Bgeei | ENSBTAG00000006342, Expressed in corpus luteum and 95 other tissues |
Family and domain databases
DisProti | DP00844 |
InterProi | View protein in InterPro IPR007648, ATPase_inhibitor_mt |
Pfami | View protein in Pfam PF04568, IATP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATIF1_BOVIN | |
Accessioni | P01096Primary (citable) accession number: P01096 Secondary accession number(s): Q2M2T4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 1, 1990 | |
Last modified: | May 25, 2022 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families