UniProtKB - P01044 (KNG1_BOVIN)
Kininogen-1
KNG1
Functioni
1 Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
GO - Molecular functioni
- cysteine-type endopeptidase inhibitor activity Source: GO_Central
- endopeptidase inhibitor activity Source: GO_Central
GO - Biological processi
- blood coagulation Source: UniProtKB-KW
- inflammatory response Source: UniProtKB-KW
- negative regulation of blood coagulation Source: GO_Central
- negative regulation of endopeptidase activity Source: GO_Central
- positive regulation of cytosolic calcium ion concentration Source: GO_Central
- vasodilation Source: UniProtKB-KW
Keywordsi
Molecular function | Protease inhibitor, Thiol protease inhibitor, Vasoactive, Vasodilator |
Biological process | Blood coagulation, Hemostasis, Inflammatory response |
Protein family/group databases
MEROPSi | I25.016 |
Names & Taxonomyi
Protein namesi | Recommended name: Kininogen-1Alternative name(s): Kininogen I Thiol proteinase inhibitor Cleaved into the following 4 chains: Alternative name(s): Kallidin I Alternative name(s): Kallidin II |
Gene namesi | Name:KNG1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: GO_Central
- extracellular space Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | 1 PublicationAdd BLAST | 18 | |
ChainiPRO_0000006675 | 19 – 621 | Kininogen-1Add BLAST | 603 | |
ChainiPRO_0000006676 | 19 – 378 | Kininogen-1 heavy chainAdd BLAST | 360 | |
PeptideiPRO_0000006677 | 379 – 388 | Lysyl-bradykinin | 10 | |
PeptideiPRO_0000006678 | 380 – 388 | Bradykinin | 9 | |
ChainiPRO_0000006679 | 389 – 621 | Kininogen-1 light chainAdd BLAST | 233 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 27 ↔ 591 | Interchain (between heavy and light chains) | ||
Glycosylationi | 47 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 82 ↔ 93 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 87 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 106 ↔ 125 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 136 | O-linked (GalNAc...) threonine; partial2 Publications | 1 | |
Disulfide bondi | 141 ↔ 144 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 168 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 169 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 197 | N-linked (GlcNAc...) asparagine; partial1 Publication | 1 | |
Glycosylationi | 204 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 205 ↔ 217 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 228 ↔ 247 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 263 ↔ 266 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 327 ↔ 339 | PROSITE-ProRule annotation1 Publication | ||
Modified residuei | 331 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 350 ↔ 369 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 398 | O-linked (GalNAc...) serine | 1 | |
Glycosylationi | 399 | O-linked (GalNAc...) threonine | 1 | |
Glycosylationi | 400 | O-linked (GalNAc...) threonine | 1 | |
Glycosylationi | 406 | O-linked (GalNAc...) serine | 1 | |
Glycosylationi | 512 | O-linked (GalNAc...) serine | 1 | |
Glycosylationi | 520 | O-linked (GalNAc...) threonine | 1 | |
Glycosylationi | 524 | O-linked (GalNAc...) threonine | 1 | |
Glycosylationi | 536 | O-linked (GalNAc...) threonine | 1 | |
Glycosylationi | 548 | O-linked (GalNAc...) threonine | 1 | |
Glycosylationi | 553 | O-linked (GalNAc...) threonine | 1 | |
Glycosylationi | 570 | O-linked (GalNAc...) threonine | 1 | |
Glycosylationi | 581 | O-linked (GalNAc...) serine1 Publication | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 378 – 379 | Cleavage; by kallikrein | 2 | |
Sitei | 388 – 389 | Cleavage; by kallikrein | 2 | |
Sitei | 498 – 499 | Cleavage; by kallikrein | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P01044 |
PeptideAtlasi | P01044 |
PRIDEi | P01044 |
PTM databases
GlyConnecti | 785, 1 O-Linked glycan (2 sites) |
iPTMneti | P01044 |
Expressioni
Tissue specificityi
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 131 | Cystatin kininogen-type 1PROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 150 – 253 | Cystatin kininogen-type 2PROSITE-ProRule annotationAdd BLAST | 104 | |
Domaini | 272 – 375 | Cystatin kininogen-type 3PROSITE-ProRule annotationAdd BLAST | 104 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 396 – 497 | DisorderedSequence analysisAdd BLAST | 102 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 410 – 435 | Basic and acidic residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 439 – 491 | Basic residuesSequence analysisAdd BLAST | 53 |
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502RYAC, Eukaryota |
HOGENOMi | CLU_029531_0_0_1 |
InParanoidi | P01044 |
OrthoDBi | 740995at2759 |
TreeFami | TF351852 |
Family and domain databases
CDDi | cd00042, CY, 3 hits |
InterProi | View protein in InterPro IPR000010, Cystatin_dom IPR046350, Cystatin_sf IPR002395, Kininogen IPR027358, Kininogen-type_cystatin_dom IPR018073, Prot_inh_cystat_CS |
Pfami | View protein in Pfam PF00031, Cystatin, 3 hits |
PRINTSi | PR00334, KININOGEN |
SMARTi | View protein in SMART SM00043, CY, 3 hits |
SUPFAMi | SSF54403, SSF54403, 3 hits |
PROSITEi | View protein in PROSITE PS00287, CYSTATIN, 2 hits PS51647, CYSTATIN_KININOGEN, 3 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKLITILFLC SRLLPSLTQE SSQEIDCNDQ DVFKAVDAAL TKYNSENKSG
60 70 80 90 100
NQFVLYRITE VARMDNPDTF YSLKYQIKEG DCPFQSNKTW QDCDYKDSAQ
110 120 130 140 150
AATGECTATV AKRGNMKFSV AIQTCLITPA EGPVVTAQYE CLGCVHPIST
160 170 180 190 200
KSPDLEPVLR YAIQYFNNNT SHSHLFDLKE VKRAQRQVVS GWNYEVNYSI
210 220 230 240 250
AQTNCSKEEF SFLTPDCKSL SSGDTGECTD KAHVDVKLRI SSFSQKCDLY
260 270 280 290 300
PVKDFVQPPT RLCAGCPKPI PVDSPDLEEP LSHSIAKLNA EHDGAFYFKI
310 320 330 340 350
DTVKKATVQV VAGLKYSIVF IARETTCSKG SNEELTKSCE INIHGQILHC
360 370 380 390 400
DANVYVVPWE EKVYPTVNCQ PLGQTSLMKR PPGFSPFRSV QVMKTEGSTT
410 420 430 440 450
VSLPHSAMSP VQDEERDSGK EQGPTHGHGW DHGKQIKLHG LGLGHKHKHD
460 470 480 490 500
QGHGHHGSHG LGHGHQKQHG LGHGHKHGHG HGKHKNKGKN NGKHYDWRTP
510 520 530 540 550
YLASSYEDST TSSAQTQEKT EETTLSSLAQ PGVAITFPDF QDSDLIATVM
560 570 580 590 600
PNTLPPHTES DDDWIPDIQT EPNSLAFKLI SDFPETTSPK CPSRPWKPVN
610 620
GVNPTVEMKE SHDFDLVDAL L
The sequence of this isoform differs from the canonical sequence as follows:
401-436: VSLPHSAMSPVQDEERDSGKEQGPTHGHGWDHGKQI → THVKSCEYKGRPQEAGAEPAPQGEVSLPAESPQLAR
437-621: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 295 | A → T in CAA23709 (PubMed:6572010).Curated | 1 | |
Sequence conflicti | 371 | P → S in AAI05500 (Ref. 3) Curated | 1 | |
Sequence conflicti | 403 | L → V in AAI05500 (Ref. 3) Curated | 1 | |
Sequence conflicti | 457 | G → R in AAI05500 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013562 | 401 – 436 | VSLPH…HGKQI → THVKSCEYKGRPQEAGAEPA PQGEVSLPAESPQLAR in isoform LMW. 1 PublicationAdd BLAST | 36 | |
Alternative sequenceiVSP_013563 | 437 – 621 | Missing in isoform LMW. 1 PublicationAdd BLAST | 185 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01491 mRNA Translation: CAA24735.1 V00426 mRNA Translation: CAA23709.1 BC105499 mRNA Translation: AAI05500.1 |
PIRi | A01281, KGBOH1 A01283, KGBOL1 |
RefSeqi | NP_786968.2, NM_175774.3 |
Genome annotation databases
GeneIDi | 280833 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01491 mRNA Translation: CAA24735.1 V00426 mRNA Translation: CAA23709.1 BC105499 mRNA Translation: AAI05500.1 |
PIRi | A01281, KGBOH1 A01283, KGBOL1 |
RefSeqi | NP_786968.2, NM_175774.3 |
3D structure databases
AlphaFoldDBi | P01044 |
SMRi | P01044 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000048995 |
Protein family/group databases
MEROPSi | I25.016 |
PTM databases
GlyConnecti | 785, 1 O-Linked glycan (2 sites) |
iPTMneti | P01044 |
Proteomic databases
PaxDbi | P01044 |
PeptideAtlasi | P01044 |
PRIDEi | P01044 |
Genome annotation databases
GeneIDi | 280833 |
Organism-specific databases
CTDi | 3827 |
Phylogenomic databases
eggNOGi | ENOG502RYAC, Eukaryota |
HOGENOMi | CLU_029531_0_0_1 |
InParanoidi | P01044 |
OrthoDBi | 740995at2759 |
TreeFami | TF351852 |
Family and domain databases
CDDi | cd00042, CY, 3 hits |
InterProi | View protein in InterPro IPR000010, Cystatin_dom IPR046350, Cystatin_sf IPR002395, Kininogen IPR027358, Kininogen-type_cystatin_dom IPR018073, Prot_inh_cystat_CS |
Pfami | View protein in Pfam PF00031, Cystatin, 3 hits |
PRINTSi | PR00334, KININOGEN |
SMARTi | View protein in SMART SM00043, CY, 3 hits |
SUPFAMi | SSF54403, SSF54403, 3 hits |
PROSITEi | View protein in PROSITE PS00287, CYSTATIN, 2 hits PS51647, CYSTATIN_KININOGEN, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KNG1_BOVIN | |
Accessioni | P01044Primary (citable) accession number: P01044 Secondary accession number(s): P01046, Q2KJ62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | May 25, 2022 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |