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Protein

Cystatin-S

Gene

CST4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Reactive site1

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI25.008

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin-S
Alternative name(s):
Cystatin-4
Cystatin-SA-III
Salivary acidic protein 1
Gene namesi
Name:CST4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101441.4
HGNCiHGNC:2476 CST4
MIMi123857 gene
neXtProtiNX_P01036

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1472
OpenTargetsiENSG00000101441
PharmGKBiPA26977

Polymorphism and mutation databases

BioMutaiCST4
DMDMi399336

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 204 PublicationsAdd BLAST20
ChainiPRO_000000665021 – 141Cystatin-SAdd BLAST121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21Phosphoserine2 Publications1
Modified residuei23Phosphoserine4 Publications1
Disulfide bondi94 ↔ 104By similarity
Disulfide bondi118 ↔ 138By similarity

Post-translational modificationi

Phosphorylated at both its N- and C-terminal regions.5 Publications

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP01036
PaxDbiP01036
PeptideAtlasiP01036
PRIDEiP01036
ProteomicsDBi51312
TopDownProteomicsiP01036

PTM databases

iPTMnetiP01036
PhosphoSitePlusiP01036
SwissPalmiP01036

Expressioni

Tissue specificityi

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid.1 Publication

Gene expression databases

BgeeiENSG00000101441 Expressed in 120 organ(s), highest expression level in saliva-secreting gland
CleanExiHS_CST4
GenevisibleiP01036 HS

Organism-specific databases

HPAiHPA043706
HPA044763

Interactioni

Protein-protein interaction databases

BioGridi107854, 49 interactors
IntActiP01036, 2 interactors
STRINGi9606.ENSP00000217423

Structurei

3D structure databases

ProteinModelPortaliP01036
SMRiP01036
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 80Secondary area of contact5

Sequence similaritiesi

Belongs to the cystatin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZZH Eukaryota
ENOG4112CFJ LUCA
GeneTreeiENSGT00900000140934
HOGENOMiHOG000231754
HOVERGENiHBG009556
InParanoidiP01036
KOiK13900
OMAiDIARTEC
OrthoDBiEOG091G0TP1
PhylomeDBiP01036

Family and domain databases

CDDicd00042 CY, 1 hit
InterProiView protein in InterPro
IPR027214 Cystatin
IPR000010 Cystatin_dom
IPR018073 Prot_inh_cystat_CS
PANTHERiPTHR11413 PTHR11413, 1 hit
PfamiView protein in Pfam
PF00031 Cystatin, 1 hit
SMARTiView protein in SMART
SM00043 CY, 1 hit
PROSITEiView protein in PROSITE
PS00287 CYSTATIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P01036-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MARPLCTLLL LMATLAGALA SSSKEENRII PGGIYDADLN DEWVQRALHF
60 70 80 90 100
AISEYNKATE DEYYRRPLQV LRAREQTFGG VNYFFDVEVG RTICTKSQPN
110 120 130 140
LDTCAFHEQP ELQKKQLCSF EIYEVPWEDR MSLVNSRCQE A
Length:141
Mass (Da):16,214
Last modified:July 1, 1993 - v3
Checksum:i65B1FEB8F074DEA6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti135N → D AA sequence (PubMed:6501254).Curated1

Mass spectrometryi

Molecular mass is 14175.8569±0.0564 Da from positions 21 - 141. Determined by ESI. 1 Publication
Molecular mass is 14255.8567±0.0899 Da from positions 21 - 141. Determined by ESI. Monophosphorylated at Ser-23, also called form S1.1 Publication
Molecular mass is 14335.811±0.0775 Da from positions 21 - 141. Determined by ESI. Diphosphorylated at Ser-21 and Ser-23, also called form S2.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04885236D → N. Corresponds to variant dbSNP:rs3210291Ensembl.1
Natural variantiVAR_03654977T → N in a breast cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54667 mRNA Translation: CAA38478.1
S51222, S51214, S51219 Genomic DNA Translation: AAB24493.1
AF319565 Genomic DNA Translation: AAK11571.1
AL359433 Genomic DNA No translation available.
BC065714 mRNA Translation: AAH65714.1
BC074952 mRNA Translation: AAH74952.1
BC074953 mRNA Translation: AAH74953.1
CCDSiCCDS13159.1
PIRiS17667 UDHUP1
RefSeqiNP_001890.1, NM_001899.2
UniGeneiHs.654549

Genome annotation databases

EnsembliENST00000217423; ENSP00000217423; ENSG00000101441
GeneIDi1472
KEGGihsa:1472
UCSCiuc002wto.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54667 mRNA Translation: CAA38478.1
S51222, S51214, S51219 Genomic DNA Translation: AAB24493.1
AF319565 Genomic DNA Translation: AAK11571.1
AL359433 Genomic DNA No translation available.
BC065714 mRNA Translation: AAH65714.1
BC074952 mRNA Translation: AAH74952.1
BC074953 mRNA Translation: AAH74953.1
CCDSiCCDS13159.1
PIRiS17667 UDHUP1
RefSeqiNP_001890.1, NM_001899.2
UniGeneiHs.654549

3D structure databases

ProteinModelPortaliP01036
SMRiP01036
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107854, 49 interactors
IntActiP01036, 2 interactors
STRINGi9606.ENSP00000217423

Protein family/group databases

MEROPSiI25.008

PTM databases

iPTMnetiP01036
PhosphoSitePlusiP01036
SwissPalmiP01036

Polymorphism and mutation databases

BioMutaiCST4
DMDMi399336

Proteomic databases

MaxQBiP01036
PaxDbiP01036
PeptideAtlasiP01036
PRIDEiP01036
ProteomicsDBi51312
TopDownProteomicsiP01036

Protocols and materials databases

DNASUi1472
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000217423; ENSP00000217423; ENSG00000101441
GeneIDi1472
KEGGihsa:1472
UCSCiuc002wto.2 human

Organism-specific databases

CTDi1472
DisGeNETi1472
EuPathDBiHostDB:ENSG00000101441.4
GeneCardsiCST4
HGNCiHGNC:2476 CST4
HPAiHPA043706
HPA044763
MIMi123857 gene
neXtProtiNX_P01036
OpenTargetsiENSG00000101441
PharmGKBiPA26977
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IZZH Eukaryota
ENOG4112CFJ LUCA
GeneTreeiENSGT00900000140934
HOGENOMiHOG000231754
HOVERGENiHBG009556
InParanoidiP01036
KOiK13900
OMAiDIARTEC
OrthoDBiEOG091G0TP1
PhylomeDBiP01036

Miscellaneous databases

ChiTaRSiCST4 human
GeneWikiiCST4
GenomeRNAii1472
PROiPR:P01036
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101441 Expressed in 120 organ(s), highest expression level in saliva-secreting gland
CleanExiHS_CST4
GenevisibleiP01036 HS

Family and domain databases

CDDicd00042 CY, 1 hit
InterProiView protein in InterPro
IPR027214 Cystatin
IPR000010 Cystatin_dom
IPR018073 Prot_inh_cystat_CS
PANTHERiPTHR11413 PTHR11413, 1 hit
PfamiView protein in Pfam
PF00031 Cystatin, 1 hit
SMARTiView protein in SMART
SM00043 CY, 1 hit
PROSITEiView protein in PROSITE
PS00287 CYSTATIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYTS_HUMAN
AccessioniPrimary (citable) accession number: P01036
Secondary accession number(s): Q9UBI5, Q9UCS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1993
Last modified: November 7, 2018
This is version 184 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
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