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Protein

Kunitz-type serine protease inhibitor homolog beta-bungarotoxin B1 chain, major component

Gene
N/A
Organism
Bungarus multicinctus (Many-banded krait)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Beta-1-bungarotoxin is a presynaptic neurotoxin of the venom. The B chain is homologous to venom basic protease inhibitors but has no protease inhibitor activity and blocks voltage-gated potassium channels (Kv) (By similarity).By similarity1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Presynaptic neurotoxin, Toxin, Voltage-gated potassium channel impairing toxin

Protein family/group databases

MEROPSiI02.978

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type serine protease inhibitor homolog beta-bungarotoxin B1 chain, major component
Alternative name(s):
Beta-1-bungarotoxin
OrganismiBungarus multicinctus (Many-banded krait)
Taxonomic identifieri8616 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeBungarinaeBungarus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi79C → S: Loss of PA2 activity. Weak loss in folding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000001686325 – 85Kunitz-type serine protease inhibitor homolog beta-bungarotoxin B1 chain, major componentAdd BLAST61

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 81PROSITE-ProRule annotation
Disulfide bondi40 ↔ 64PROSITE-ProRule annotation
Disulfide bondi56 ↔ 77PROSITE-ProRule annotation
Disulfide bondi79Interchain (with an A chain)

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Heterodimer; disulfide-linked. The A chains have phospholipase A2 activity and the B chains show homology with the basic protease inhibitors.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP00987
SMRiP00987
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 81BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST51

Sequence similaritiesi

Belongs to the venom Kunitz-type family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd00109 KU, 1 hit
Gene3Di4.10.410.10, 1 hit
InterProiView protein in InterPro
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR020901 Prtase_inh_Kunz-CS
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00131 KU, 1 hit
SUPFAMiSSF57362 SSF57362, 1 hit
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 1 hit
PS50279 BPTI_KUNITZ_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00987-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSGGLLLLL GLLTLCAELI PVSSRQRHRD CDKPPDKGNC GPVRRAFYYD
60 70 80
TRLKTCKAFQ YRGCNGNGNH FKTETLCRCE CLVYP
Length:85
Mass (Da):9,571
Last modified:July 15, 1998 - v2
Checksum:iA1E3D452AE67DE5C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16C → S in CAB62503 (PubMed:10903499).Curated1
Sequence conflicti45Missing AA sequence (PubMed:624701).Curated1
Sequence conflicti65 – 70NGNGNH → DGDHGN AA sequence (PubMed:624701).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12100 mRNA Translation: CAA72809.1
AJ251223 Genomic DNA Translation: CAB62503.1
PIRiA01219 TIKFBY

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12100 mRNA Translation: CAA72809.1
AJ251223 Genomic DNA Translation: CAB62503.1
PIRiA01219 TIKFBY

3D structure databases

ProteinModelPortaliP00987
SMRiP00987
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI02.978

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd00109 KU, 1 hit
Gene3Di4.10.410.10, 1 hit
InterProiView protein in InterPro
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR020901 Prtase_inh_Kunz-CS
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00131 KU, 1 hit
SUPFAMiSSF57362 SSF57362, 1 hit
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 1 hit
PS50279 BPTI_KUNITZ_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVKTH1_BUNMU
AccessioniPrimary (citable) accession number: P00987
Secondary accession number(s): O42298, P00988, Q9PTA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 15, 1998
Last modified: November 22, 2017
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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