Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kunitz-type serine protease inhibitor homolog alpha-dendrotoxin

Gene
N/A
Organism
Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine protease inhibitor homolog that blocks voltage-gated potassium channels (Kv1.1/KCNA1, Kv1.2/KCNA2, and Kv1.6/KCNA6) (IC50=0.4-150 nM) and facilitates neurotransmitter release.2 Publications

Miscellaneous

Does not inhibit serine proteases and voltage-gated potassium channels Kvl.3/KCNA3, Kv1.4/KCNA4, Kv1.5/KCNA5, Kv3.1/KCNC1, Kv3.4/KCNC4, and Kv4.1/KCND1.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei5May be the major determinant of the binding affinity for potassium channels1
Sitei9Important for binding to potassium channels1
Sitei19Not important for inhibition of potassium channels1

GO - Molecular functioni

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type serine protease inhibitor homolog alpha-dendrotoxin
Short name:
Alpha-DTX
Alternative name(s):
Protease inhibitor 1 homolog
Toxin C13S2C3
Venom basic protease inhibitor 1 homolog
OrganismiDendroaspis angusticeps (Eastern green mamba) (Naja angusticeps)
Taxonomic identifieri8618 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeDendroaspis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 23 mg/kg by intravenous injection.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001554331 – 59Kunitz-type serine protease inhibitor homolog alpha-dendrotoxinAdd BLAST59

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi7 ↔ 57PROSITE-ProRule annotation1 Publication
Disulfide bondi16 ↔ 40PROSITE-ProRule annotation1 Publication
Disulfide bondi32 ↔ 53PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

159
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4
Beta strandi15 – 17Combined sources3
Beta strandi20 – 26Combined sources7
Turni27 – 30Combined sources4
Beta strandi31 – 37Combined sources7
Beta strandi47 – 49Combined sources3
Helixi50 – 57Combined sources8

3D structure databases

ProteinModelPortaliP00980
SMRiP00980
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00980

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 57BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST51

Sequence similaritiesi

Belongs to the venom Kunitz-type family.Curated

Phylogenomic databases

HOVERGENiHBG006193

Family and domain databases

CDDicd00109 KU, 1 hit
Gene3Di4.10.410.10, 1 hit
InterProiView protein in InterPro
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR020901 Prtase_inh_Kunz-CS
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00131 KU, 1 hit
SUPFAMiSSF57362 SSF57362, 1 hit
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 1 hit
PS50279 BPTI_KUNITZ_2, 1 hit

Sequencei

Sequence statusi: Complete.

P00980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QPRRKLCILH RNPGRCYDKI PAFYYNQKKK QCERFDWSGC GGNSNRFKTI

EECRRTCIG
Length:59
Mass (Da):7,071
Last modified:July 21, 1986 - v1
Checksum:i96B60752E8AD81AE
GO

Sequence databases

PIRiA01212 VIEPIA

Similar proteinsi

Entry informationi

Entry nameiVKTHA_DENAN
AccessioniPrimary (citable) accession number: P00980
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 22, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health