Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pancreatic trypsin inhibitor

Gene
N/A
Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei50 – 51Reactive bond for trypsin2

GO - Molecular functioni

  • calcium ion binding Source: CAFA
  • potassium channel inhibitor activity Source: AgBase
  • protease binding Source: CAFA
  • protein heterodimerization activity Source: CAFA
  • serine-type endopeptidase inhibitor activity Source: CAFA
  • sulfate binding Source: CAFA
  • zymogen binding Source: CAFA

GO - Biological processi

  • negative regulation of platelet aggregation Source: CACAO
  • negative regulation of serine-type endopeptidase activity Source: CAFA
  • negative regulation of thrombin-activated receptor signaling pathway Source: CACAO
  • trypsinogen activation Source: CAFA

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.001

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic trypsin inhibitor
Alternative name(s):
Aprotinin
Basic protease inhibitor
Short name:
BPI
Short name:
BPTI
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Previously available under the name Trasylol (Mile). Used for inhibiting coagulation so as to reduce blood loss during bypass surgery.

Protein family/group databases

Allergomei3890 Bos d TI

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000001685222 – 35Add BLAST14
ChainiPRO_000001685336 – 93Pancreatic trypsin inhibitorAdd BLAST58
PropeptideiPRO_000001685494 – 1007

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 90
Disulfide bondi49 ↔ 73PROSITE-ProRule annotation1 Publication
Disulfide bondi65 ↔ 86PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP00974

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • protease binding Source: CAFA
  • protein heterodimerization activity Source: CAFA
  • zymogen binding Source: CAFA

Protein-protein interaction databases

DIPiDIP-6113N
IntActiP00974, 8 interactors
MINTiP00974

Structurei

Secondary structure

1100
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 41Combined sources4
Beta strandi48 – 50Combined sources3
Beta strandi53 – 59Combined sources7
Turni60 – 63Combined sources4
Beta strandi64 – 70Combined sources7
Beta strandi72 – 74Combined sources3
Beta strandi76 – 78Combined sources3
Beta strandi80 – 82Combined sources3
Helixi83 – 90Combined sources8

3D structure databases

DisProtiDP00729
ProteinModelPortaliP00974
SMRiP00974
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00974

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 90BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000007327
HOVERGENiHBG006193

Family and domain databases

CDDicd00109 KU, 1 hit
Gene3Di4.10.410.10, 1 hit
InterProiView protein in InterPro
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR020901 Prtase_inh_Kunz-CS
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00131 KU, 1 hit
SUPFAMiSSF57362 SSF57362, 1 hit
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 1 hit
PS50279 BPTI_KUNITZ_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMSRLCLSV ALLVLLGTLA ASTPGCDTSN QAKAQRPDFC LEPPYTGPCK
60 70 80 90 100
ARIIRYFYNA KAGLCQTFVY GGCRAKRNNF KSAEDCMRTC GGAIGPWENL
Length:100
Mass (Da):10,903
Last modified:July 1, 1989 - v2
Checksum:i6A778A4AD763FB19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20934, M20930, M20932 Genomic DNA Translation: AAD13685.1
X03365 Genomic DNA Translation: CAA27062.1 Sequence problems.
X03365 Genomic DNA Translation: CAA27063.1
X05274 mRNA Translation: CAA28886.1
PIRiS00277 TIBO
UniGeneiBt.102246
Bt.28518

Similar proteinsi

Entry informationi

Entry nameiBPT1_BOVIN
AccessioniPrimary (citable) accession number: P00974
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: July 18, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health