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UniProtKB - P00974 (BPT1_BOVIN)
Protein
Pancreatic trypsin inhibitor
Gene
N/A
Organism
Bos taurus (Bovine)
Status
Functioni
Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 50 – 51 | Reactive bond for trypsin | 2 |
GO - Molecular functioni
- calcium ion binding Source: CAFA
- potassium channel inhibitor activity Source: AgBase
- protease binding Source: CAFA
- serine-type endopeptidase inhibitor activity Source: CAFA
- sulfate binding Source: CAFA
- zymogen binding Source: CAFA
GO - Biological processi
- negative regulation of platelet aggregation Source: CACAO
- negative regulation of serine-type endopeptidase activity Source: CAFA
- negative regulation of thrombin-activated receptor signaling pathway Source: CACAO
- trypsinogen activation Source: CAFA
Keywordsi
Molecular function | Protease inhibitor, Serine protease inhibitor |
Protein family/group databases
MEROPSi | I02.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Pancreatic trypsin inhibitorAlternative name(s): Aprotinin Basic protease inhibitor Short name: BPI Short name: BPTI |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: GO_Central
Other locations
- serine protease inhibitor complex Source: CAFA
Keywords - Cellular componenti
SecretedPathology & Biotechi
Pharmaceutical usei
Previously available under the name Trasylol (Mile). Used for inhibiting coagulation so as to reduce blood loss during bypass surgery.
Protein family/group databases
Allergomei | 3890, Bos d TI |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
PropeptideiPRO_0000016852 | 22 – 35 | Add BLAST | 14 | |
ChainiPRO_0000016853 | 36 – 93 | Pancreatic trypsin inhibitorAdd BLAST | 58 | |
PropeptideiPRO_0000016854 | 94 – 100 | 7 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 40 ↔ 90 | |||
Disulfide bondi | 49 ↔ 73 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 65 ↔ 86 | PROSITE-ProRule annotation1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
CPTACi | CPTAC-1472 |
PRIDEi | P00974 |
Interactioni
Binary interactionsi
P00974
With | #Exp. | IntAct |
---|---|---|
P00760 | 4 | EBI-1032263,EBI-986385 |
Q52V24 from Astacus leptodactylus. | 5 | EBI-1032263,EBI-8755401 |
GO - Molecular functioni
- protease binding Source: CAFA
- zymogen binding Source: CAFA
Protein-protein interaction databases
DIPi | DIP-6113N |
IntActi | P00974, 5 interactors |
MINTi | P00974 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P00974 |
PCDDBi | P00974 |
SASBDBi | P00974 |
SMRi | P00974 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00974 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 40 – 90 | BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST | 51 |
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00109, KU, 1 hit |
DisProti | DP00729 |
Gene3Di | 4.10.410.10, 1 hit |
InterProi | View protein in InterPro IPR002223, Kunitz_BPTI IPR036880, Kunitz_BPTI_sf IPR020901, Prtase_inh_Kunz-CS |
Pfami | View protein in Pfam PF00014, Kunitz_BPTI, 1 hit |
PRINTSi | PR00759, BASICPTASE |
SMARTi | View protein in SMART SM00131, KU, 1 hit |
SUPFAMi | SSF57362, SSF57362, 1 hit |
PROSITEi | View protein in PROSITE PS00280, BPTI_KUNITZ_1, 1 hit PS50279, BPTI_KUNITZ_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00974-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKMSRLCLSV ALLVLLGTLA ASTPGCDTSN QAKAQRPDFC LEPPYTGPCK
60 70 80 90 100
ARIIRYFYNA KAGLCQTFVY GGCRAKRNNF KSAEDCMRTC GGAIGPWENL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20934, M20930, M20932 Genomic DNA Translation: AAD13685.1 X03365 Genomic DNA Translation: CAA27062.1 Sequence problems. X03365 Genomic DNA Translation: CAA27063.1 X05274 mRNA Translation: CAA28886.1 |
PIRi | S00277, TIBO |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20934, M20930, M20932 Genomic DNA Translation: AAD13685.1 X03365 Genomic DNA Translation: CAA27062.1 Sequence problems. X03365 Genomic DNA Translation: CAA27063.1 X05274 mRNA Translation: CAA28886.1 |
PIRi | S00277, TIBO |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AAL | X-ray | 1.60 | A/B | 36-93 | [»] | |
1B0C | X-ray | 2.80 | A/B/C/D/E | 36-91 | [»] | |
1BHC | X-ray | 2.70 | A/B/C/D/E/F/G/H/I/J | 36-93 | [»] | |
1BPI | X-ray | 1.09 | A | 36-93 | [»] | |
1BPT | X-ray | 2.00 | A | 36-93 | [»] | |
1BRB | X-ray | 2.10 | I | 36-93 | [»] | |
1BTH | X-ray | 2.30 | P/Q | 36-93 | [»] | |
1BTI | X-ray | 2.20 | A | 36-93 | [»] | |
1BZ5 | X-ray | 2.58 | A/B/C/D/E | 36-93 | [»] | |
1BZX | X-ray | 2.10 | I | 36-93 | [»] | |
1CBW | X-ray | 2.60 | D/I | 36-93 | [»] | |
1CO7 | X-ray | 1.90 | I | 2-100 | [»] | |
1D0D | X-ray | 1.62 | B | 36-93 | [»] | |
1EAW | X-ray | 2.93 | B/D | 36-93 | [»] | |
1EJM | X-ray | 1.85 | B/D/F | 36-93 | [»] | |
1F5R | X-ray | 1.65 | I | 36-100 | [»] | |
1F7Z | X-ray | 1.55 | I | 36-100 | [»] | |
1FAK | X-ray | 2.10 | I | 37-90 | [»] | |
1FAN | X-ray | 2.00 | A | 36-93 | [»] | |
1FY8 | X-ray | 1.70 | I | 36-93 | [»] | |
1G6X | X-ray | 0.86 | A | 36-93 | [»] | |
1JV8 | NMR | - | A | 36-93 | [»] | |
1JV9 | NMR | - | A | 36-93 | [»] | |
1K09 | NMR | - | A/B | 50-73 | [»] | |
1K6U | X-ray | 1.00 | A | 36-93 | [»] | |
1LD5 | NMR | - | A | 36-93 | [»] | |
1LD6 | NMR | - | A | 36-93 | [»] | |
1MTN | X-ray | 2.80 | D/H | 36-93 | [»] | |
1NAG | X-ray | 1.90 | A | 36-93 | [»] | |
1OA5 | NMR | - | 5 | 36-93 | [»] | |
1OA6 | NMR | - | 5 | 36-93 | [»] | |
1P2I | X-ray | 1.65 | I | 36-93 | [»] | |
1P2J | X-ray | 1.35 | I | 36-93 | [»] | |
1P2K | X-ray | 1.60 | I | 36-93 | [»] | |
1P2M | X-ray | 1.75 | B/D | 36-93 | [»] | |
1P2N | X-ray | 1.80 | B/D | 36-93 | [»] | |
1P2O | X-ray | 2.00 | B/D | 36-93 | [»] | |
1P2Q | X-ray | 1.80 | B/D | 36-93 | [»] | |
1PIT | NMR | - | A | 36-93 | [»] | |
1QLQ | X-ray | 1.42 | A | 36-93 | [»] | |
1T7C | X-ray | 1.85 | B/D | 36-93 | [»] | |
1T8L | X-ray | 1.75 | B/D | 36-93 | [»] | |
1T8M | X-ray | 1.80 | B/D | 36-93 | [»] | |
1T8N | X-ray | 1.75 | B/D | 36-93 | [»] | |
1T8O | X-ray | 1.70 | B/D | 36-93 | [»] | |
1TPA | X-ray | 1.90 | I | 36-93 | [»] | |
1UUA | NMR | - | A | 38-93 | [»] | |
1UUB | NMR | - | A | 38-93 | [»] | |
1YKT | X-ray | 1.70 | B | 36-91 | [»] | |
1YLC | X-ray | 1.70 | B | 36-91 | [»] | |
1YLD | X-ray | 1.70 | B | 36-91 | [»] | |
2FI3 | X-ray | 1.58 | I | 36-93 | [»] | |
2FI4 | X-ray | 1.58 | I | 36-93 | [»] | |
2FI5 | X-ray | 1.58 | I | 36-93 | [»] | |
2FTL | X-ray | 1.62 | I | 36-93 | [»] | |
2FTM | X-ray | 1.65 | B | 36-93 | [»] | |
2HEX | X-ray | 2.10 | A/B/C/D/E | 36-93 | [»] | |
2IJO | X-ray | 2.30 | I | 36-93 | [»] | |
2KAI | X-ray | 2.50 | I | 36-93 | [»] | |
2PTC | X-ray | 1.90 | I | 36-93 | [»] | |
2R9P | X-ray | 1.40 | E/F/G/I | 36-93 | [»] | |
2RA3 | X-ray | 1.46 | C/I | 36-93 | [»] | |
2TGP | X-ray | 1.90 | I | 36-93 | [»] | |
2TPI | X-ray | 2.10 | I | 36-93 | [»] | |
2ZJX | X-ray | 1.09 | A/B | 36-93 | [»] | |
2ZVX | X-ray | 1.09 | A/B | 36-93 | [»] | |
3BTD | X-ray | 1.90 | I | 36-93 | [»] | |
3BTE | X-ray | 1.85 | I | 36-93 | [»] | |
3BTF | X-ray | 1.80 | I | 36-93 | [»] | |
3BTG | X-ray | 1.90 | I | 36-93 | [»] | |
3BTH | X-ray | 1.75 | I | 36-93 | [»] | |
3BTK | X-ray | 1.85 | I | 36-93 | [»] | |
3BTM | X-ray | 1.80 | I | 36-93 | [»] | |
3BTQ | X-ray | 1.90 | I | 36-93 | [»] | |
3BTT | X-ray | 1.90 | I | 36-93 | [»] | |
3BTW | X-ray | 2.05 | I | 36-93 | [»] | |
3FP6 | X-ray | 1.49 | I | 36-93 | [»] | |
3FP7 | X-ray | 1.46 | I | 36-50 | [»] | |
J | 51-93 | [»] | ||||
3FP8 | X-ray | 1.46 | I | 36-93 | [»] | |
3GYM | X-ray | 2.80 | I/J | 36-93 | [»] | |
3LDI | X-ray | 2.20 | A/B/C/D/E | 36-93 | [»] | |
3LDJ | X-ray | 1.70 | A/B/C | 36-93 | [»] | |
3LDM | X-ray | 2.60 | A/B/C/D/E | 36-93 | [»] | |
3OTJ | Other | 2.15 | I | 36-93 | [»] | |
3P92 | X-ray | 1.60 | E | 36-93 | [»] | |
3P95 | X-ray | 1.30 | E | 36-93 | [»] | |
3TGI | X-ray | 1.80 | I | 36-100 | [»] | |
3TGJ | X-ray | 2.20 | I | 36-100 | [»] | |
3TGK | X-ray | 1.70 | I | 36-100 | [»] | |
3TPI | X-ray | 1.90 | I | 36-93 | [»] | |
3U1J | X-ray | 1.80 | E | 36-93 | [»] | |
3WNY | X-ray | 1.30 | A/B/C/E/F/G/H/I | 36-95 | [»] | |
4BNR | X-ray | 2.00 | I/J | 1-100 | [»] | |
4DG4 | X-ray | 1.40 | C/E/F/H | 36-93 | [»] | |
4PTI | X-ray | 1.50 | A | 36-93 | [»] | |
4TPI | X-ray | 2.20 | I | 36-93 | [»] | |
4WWY | X-ray | 1.70 | C/I | 36-93 | [»] | |
4WXV | X-ray | 2.10 | C/I | 36-90 | [»] | |
4Y0Y | X-ray | 1.25 | I | 36-93 | [»] | |
4Y0Z | X-ray | 1.37 | I | 36-93 | [»] | |
4Y10 | X-ray | 1.37 | I | 36-93 | [»] | |
4Y11 | X-ray | 1.30 | I | 36-93 | [»] | |
5JB4 | X-ray | 1.99 | A/B/C | 36-93 | [»] | |
5JB5 | X-ray | 1.60 | A/B/C | 36-93 | [»] | |
5JB6 | X-ray | 1.90 | A/B/C | 36-93 | [»] | |
5JB7 | X-ray | 1.90 | A/B/C | 36-93 | [»] | |
5PTI | X-ray | 1.00 | A | 36-93 | [»] | |
5XX2 | X-ray | 1.12 | A/B | 36-93 | [»] | |
5XX3 | X-ray | 1.12 | A/B | 36-93 | [»] | |
5XX4 | X-ray | 1.67 | A/B | 36-93 | [»] | |
5XX5 | X-ray | 1.38 | A/B | 36-93 | [»] | |
5XX6 | X-ray | 1.31 | A/B | 36-93 | [»] | |
5XX7 | X-ray | 1.38 | A/D | 36-93 | [»] | |
5XX8 | X-ray | 1.30 | A/B | 36-93 | [»] | |
5YVU | X-ray | 2.49 | I | 36-90 | [»] | |
5YW1 | X-ray | 2.60 | I | 36-90 | [»] | |
6F1F | X-ray | 1.72 | A/B/C/D/E | 36-93 | [»] | |
6PTI | X-ray | 1.70 | A | 36-93 | [»] | |
7PTI | X-ray | 1.60 | A | 36-93 | [»] | |
8PTI | X-ray | 1.80 | A | 36-93 | [»] | |
9PTI | X-ray | 1.22 | A | 36-93 | [»] | |
BMRBi | P00974 | |||||
PCDDBi | P00974 | |||||
SASBDBi | P00974 | |||||
SMRi | P00974 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-6113N |
IntActi | P00974, 5 interactors |
MINTi | P00974 |
Protein family/group databases
Allergomei | 3890, Bos d TI |
MEROPSi | I02.001 |
Proteomic databases
CPTACi | CPTAC-1472 |
PRIDEi | P00974 |
Miscellaneous databases
EvolutionaryTracei | P00974 |
Family and domain databases
CDDi | cd00109, KU, 1 hit |
DisProti | DP00729 |
Gene3Di | 4.10.410.10, 1 hit |
InterProi | View protein in InterPro IPR002223, Kunitz_BPTI IPR036880, Kunitz_BPTI_sf IPR020901, Prtase_inh_Kunz-CS |
Pfami | View protein in Pfam PF00014, Kunitz_BPTI, 1 hit |
PRINTSi | PR00759, BASICPTASE |
SMARTi | View protein in SMART SM00131, KU, 1 hit |
SUPFAMi | SSF57362, SSF57362, 1 hit |
PROSITEi | View protein in PROSITE PS00280, BPTI_KUNITZ_1, 1 hit PS50279, BPTI_KUNITZ_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BPT1_BOVIN | |
Accessioni | P00974Primary (citable) accession number: P00974 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 1, 1989 | |
Last modified: | February 23, 2022 | |
This is version 179 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Pharmaceutical, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references