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UniProtKB - P00962 (SYQ_ECOLI)
Protein
Glutamine--tRNA ligase
Gene
glnS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- ATP + L-glutamine + tRNAGln = AMP + diphosphate + L-glutaminyl-tRNAGlnUniRule annotation5 PublicationsEC:6.1.1.18UniRule annotation5 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 67 | L-glutamineUniRule annotationCombined sources3 Publications | 1 | |
Binding sitei | 212 | L-glutamineUniRule annotationCombined sources4 Publications | 1 | |
Binding sitei | 231 | ATPUniRule annotationCombined sources3 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 35 – 37 | ATPUniRule annotationCombined sources1 Publication | 3 | |
Nucleotide bindingi | 41 – 47 | ATPUniRule annotationCombined sources1 Publication | 7 | |
Nucleotide bindingi | 261 – 262 | ATPUniRule annotationCombined sources3 Publications | 2 | |
Nucleotide bindingi | 269 – 271 | ATPUniRule annotationCombined sources3 Publications | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- glutamine-tRNA ligase activity Source: EcoCyc
GO - Biological processi
- glutaminyl-tRNA aminoacylation Source: EcoCyc
- glutamyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GLNS-MONOMER |
BRENDAi | 6.1.1.18, 2026 6.1.1.24, 2026 |
SABIO-RKi | P00962 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamine--tRNA ligaseUniRule annotation (EC:6.1.1.18UniRule annotation5 Publications)Alternative name(s): Glutaminyl-tRNA synthetaseUniRule annotation Short name: GlnRSUniRule annotation |
Gene namesi | Name:glnSUniRule annotation Ordered Locus Names:b0680, JW0666 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | R → A or K: Decreased affinity for glutamine and catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 230 | C → R: Decreases catalytic activity 1000-fold, but has no effect on affinity for glutamine. Loss of catalytic activity; when associated with I-256. 1 Publication | 1 | |
Mutagenesisi | 256 | Q → I: Loss of catalytic activity; when associated with R-230. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000195833 | 2 – 554 | Glutamine--tRNA ligaseAdd BLAST | 553 |
Proteomic databases
jPOSTi | P00962 |
PaxDbi | P00962 |
PRIDEi | P00962 |
2D gel databases
SWISS-2DPAGEi | P00962 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 4261207, 52 interactors |
DIPi | DIP-9787N |
IntActi | P00962, 5 interactors |
STRINGi | 511145.b0680 |
Chemistry databases
BindingDBi | P00962 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P00962 |
SMRi | P00962 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00962 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 317 – 324 | Interaction with tRNAUniRule annotation2 Publications | 8 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 34 – 44 | 'HIGH' regionUniRule annotationAdd BLAST | 11 | |
Motifi | 268 – 272 | 'KMSKS' regionUniRule annotation | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0008, Bacteria |
HOGENOMi | CLU_001882_2_3_6 |
InParanoidi | P00962 |
OMAi | INNFCAQ |
PhylomeDBi | P00962 |
Family and domain databases
Gene3Di | 1.10.1160.10, 1 hit 2.40.240.10, 2 hits 3.40.50.620, 1 hit |
HAMAPi | MF_00126, Gln_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR004514, Gln-tRNA-synth IPR022861, Gln_tRNA_ligase_bac IPR000924, Glu/Gln-tRNA-synth IPR020058, Glu/Gln-tRNA-synth_Ib_cat-dom IPR020059, Glu/Gln-tRNA-synth_Ib_codon-bd IPR020061, Glu_tRNA_lig_a-bdl IPR020056, Rbsml_L25/Gln-tRNA_synth_N IPR011035, Ribosomal_L25/Gln-tRNA_synth IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00749, tRNA-synt_1c, 1 hit PF03950, tRNA-synt_1c_C, 1 hit |
PRINTSi | PR00987, TRNASYNTHGLU |
SUPFAMi | SSF50715, SSF50715, 1 hit |
TIGRFAMsi | TIGR00440, glnS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00962-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEAEARPTN FIRQIIDEDL ASGKHTTVHT RFPPEPNGYL HIGHAKSICL
60 70 80 90 100
NFGIAQDYKG QCNLRFDDTN PVKEDIEYVE SIKNDVEWLG FHWSGNVRYS
110 120 130 140 150
SDYFDQLHAY AIELINKGLA YVDELTPEQI REYRGTLTQP GKNSPYRDRS
160 170 180 190 200
VEENLALFEK MRAGGFEEGK ACLRAKIDMA SPFIVMRDPV LYRIKFAEHH
210 220 230 240 250
QTGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL YDWVLDNITI
260 270 280 290 300
PVHPRQYEFS RLNLEYTVMS KRKLNLLVTD KHVEGWDDPR MPTISGLRRR
310 320 330 340 350
GYTAASIREF CKRIGVTKQD NTIEMASLES CIREDLNENA PRAMAVIDPV
360 370 380 390 400
KLVIENYQGE GEMVTMPNHP NKPEMGSRQV PFSGEIWIDR ADFREEANKQ
410 420 430 440 450
YKRLVLGKEV RLRNAYVIKA ERVEKDAEGN ITTIFCTYDA DTLSKDPADG
460 470 480 490 500
RKVKGVIHWV SAAHALPVEI RLYDRLFSVP NPGAADDFLS VINPESLVIK
510 520 530 540 550
QGFAEPSLKD AVAGKAFQFE REGYFCLDSR HSTAEKPVFN RTVGLRDTWA
KVGE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 549 – 554 | WAKVGE → GRK (PubMed:6288695).Curated | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01575 Genomic DNA Translation: CAA24894.1 U00096 Genomic DNA Translation: AAC73774.1 AP009048 Genomic DNA Translation: BAA35328.1 M16470, M16368 Genomic DNA Translation: AAA69006.1 |
PIRi | G64802, SYECQT I41235 |
RefSeqi | NP_415206.1, NC_000913.3 WP_001287154.1, NZ_STEB01000044.1 |
Genome annotation databases
EnsemblBacteriai | AAC73774; AAC73774; b0680 BAA35328; BAA35328; BAA35328 |
GeneIDi | 66671050 945310 |
KEGGi | ecj:JW0666 eco:b0680 |
PATRICi | fig|1411691.4.peg.1596 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01575 Genomic DNA Translation: CAA24894.1 U00096 Genomic DNA Translation: AAC73774.1 AP009048 Genomic DNA Translation: BAA35328.1 M16470, M16368 Genomic DNA Translation: AAA69006.1 |
PIRi | G64802, SYECQT I41235 |
RefSeqi | NP_415206.1, NC_000913.3 WP_001287154.1, NZ_STEB01000044.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EUQ | X-ray | 3.10 | A | 1-548 | [»] | |
1EUY | X-ray | 2.60 | A | 1-548 | [»] | |
1EXD | X-ray | 2.70 | A | 1-548 | [»] | |
1GSG | X-ray | 2.80 | P | 2-554 | [»] | |
1GTR | X-ray | 2.50 | A | 2-554 | [»] | |
1GTS | X-ray | 2.80 | A | 2-554 | [»] | |
1NYL | X-ray | 2.60 | A | 9-547 | [»] | |
1O0B | X-ray | 2.70 | A | 2-554 | [»] | |
1O0C | X-ray | 2.70 | A | 2-554 | [»] | |
1QRS | X-ray | 2.60 | A | 2-554 | [»] | |
1QRT | X-ray | 2.70 | A | 2-554 | [»] | |
1QRU | X-ray | 3.00 | A | 2-554 | [»] | |
1QTQ | X-ray | 2.25 | A | 2-554 | [»] | |
1ZJW | X-ray | 2.50 | A | 2-554 | [»] | |
2RD2 | X-ray | 2.60 | A | 1-548 | [»] | |
2RE8 | X-ray | 2.60 | A | 1-548 | [»] | |
4JXX | X-ray | 2.30 | A | 2-554 | [»] | |
4JXZ | X-ray | 2.40 | A | 2-554 | [»] | |
4JYZ | X-ray | 2.50 | A | 2-554 | [»] | |
AlphaFoldDBi | P00962 | |||||
SMRi | P00962 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261207, 52 interactors |
DIPi | DIP-9787N |
IntActi | P00962, 5 interactors |
STRINGi | 511145.b0680 |
Chemistry databases
BindingDBi | P00962 |
2D gel databases
SWISS-2DPAGEi | P00962 |
Proteomic databases
jPOSTi | P00962 |
PaxDbi | P00962 |
PRIDEi | P00962 |
Genome annotation databases
EnsemblBacteriai | AAC73774; AAC73774; b0680 BAA35328; BAA35328; BAA35328 |
GeneIDi | 66671050 945310 |
KEGGi | ecj:JW0666 eco:b0680 |
PATRICi | fig|1411691.4.peg.1596 |
Organism-specific databases
EchoBASEi | EB0385 |
Phylogenomic databases
eggNOGi | COG0008, Bacteria |
HOGENOMi | CLU_001882_2_3_6 |
InParanoidi | P00962 |
OMAi | INNFCAQ |
PhylomeDBi | P00962 |
Enzyme and pathway databases
BioCyci | EcoCyc:GLNS-MONOMER |
BRENDAi | 6.1.1.18, 2026 6.1.1.24, 2026 |
SABIO-RKi | P00962 |
Miscellaneous databases
EvolutionaryTracei | P00962 |
PROi | PR:P00962 |
Family and domain databases
Gene3Di | 1.10.1160.10, 1 hit 2.40.240.10, 2 hits 3.40.50.620, 1 hit |
HAMAPi | MF_00126, Gln_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR004514, Gln-tRNA-synth IPR022861, Gln_tRNA_ligase_bac IPR000924, Glu/Gln-tRNA-synth IPR020058, Glu/Gln-tRNA-synth_Ib_cat-dom IPR020059, Glu/Gln-tRNA-synth_Ib_codon-bd IPR020061, Glu_tRNA_lig_a-bdl IPR020056, Rbsml_L25/Gln-tRNA_synth_N IPR011035, Ribosomal_L25/Gln-tRNA_synth IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00749, tRNA-synt_1c, 1 hit PF03950, tRNA-synt_1c_C, 1 hit |
PRINTSi | PR00987, TRNASYNTHGLU |
SUPFAMi | SSF50715, SSF50715, 1 hit |
TIGRFAMsi | TIGR00440, glnS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYQ_ECOLI | |
Accessioni | P00962Primary (citable) accession number: P00962 Secondary accession number(s): Q59403 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 209 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families