UniProtKB - P00961 (SYGB_ECOLI)
Protein
Glycine--tRNA ligase beta subunit
Gene
glyS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- EC:6.1.1.14
GO - Molecular functioni
- arginine-tRNA ligase activity Source: InterPro
- ATP binding Source: UniProtKB-UniRule
- glycine-tRNA ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- arginyl-tRNA aminoacylation Source: InterPro
- glycyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GLYS-MONOMER MetaCyc:GLYS-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glycine--tRNA ligase beta subunit (EC:6.1.1.14)Alternative name(s): Glycyl-tRNA synthetase beta subunit Short name: GlyRS |
Gene namesi | Name:glyS Synonyms:glyS(B) Ordered Locus Names:b3559, JW3530 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000072899 | 2 – 689 | Glycine--tRNA ligase beta subunitAdd BLAST | 688 |
Proteomic databases
jPOSTi | P00961 |
PaxDbi | P00961 |
PRIDEi | P00961 |
Interactioni
Subunit structurei
Tetramer of two alpha and two beta subunits.
Binary interactionsi
P00961
With | #Exp. | IntAct |
---|---|---|
glyQ [P00960] | 3 | EBI-551400,EBI-551191 |
Protein-protein interaction databases
BioGRIDi | 4259716, 23 interactors 852388, 1 interactor |
ComplexPortali | CPX-5201, Glycyl-tRNA synthetase complex |
DIPi | DIP-6880N |
IntActi | P00961, 11 interactors |
STRINGi | 511145.b3559 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | COG0751, Bacteria |
HOGENOMi | CLU_007220_2_2_6 |
InParanoidi | P00961 |
PhylomeDBi | P00961 |
Family and domain databases
HAMAPi | MF_00255, Gly_tRNA_synth_beta, 1 hit |
InterProi | View protein in InterPro IPR008909, DALR_anticod-bd IPR015944, Gly-tRNA-synth_bsu IPR006194, Gly-tRNA-synth_heterodimer |
PANTHERi | PTHR30075, PTHR30075, 1 hit |
Pfami | View protein in Pfam PF05746, DALR_1, 1 hit PF02092, tRNA_synt_2f, 1 hit |
PRINTSi | PR01045, TRNASYNTHGB |
TIGRFAMsi | TIGR00211, glyS, 1 hit |
PROSITEi | View protein in PROSITE PS50861, AA_TRNA_LIGASE_II_GLYAB, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00961-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEKTFLVEI GTEELPPKAL RSLAESFAAN FTAELDNAGL AHGTVQWFAA
60 70 80 90 100
PRRLALKVAN LAEAQPDREI EKRGPAIAQA FDAEGKPSKA AEGWARGCGI
110 120 130 140 150
TVDQAERLTT DKGEWLLYRA HVKGESTEAL LPNMVATSLA KLPIPKLMRW
160 170 180 190 200
GASDVHFVRP VHTVTLLLGD KVIPATILGI QSDRVIRGHR FMGEPEFTID
210 220 230 240 250
NADQYPEILR ERGKVIADYE ERKAKIKADA EEAARKIGGN ADLSESLLEE
260 270 280 290 300
VASLVEWPVV LTAKFEEKFL AVPAEALVYT MKGDQKYFPV YANDGKLLPN
310 320 330 340 350
FIFVANIESK DPQQIISGNE KVVRPRLADA EFFFNTDRKK RLEDNLPRLQ
360 370 380 390 400
TVLFQQQLGT LRDKTDRIQA LAGWIAEQIG ADVNHATRAG LLSKCDLMTN
410 420 430 440 450
MVFEFTDTQG VMGMHYARHD GEAEDVAVAL NEQYQPRFAG DDLPSNPVAC
460 470 480 490 500
ALAIADKMDT LAGIFGIGQH PKGDKDPFAL RRAALGVLRI IVEKNLNLDL
510 520 530 540 550
QTLTEEAVRL YGDKLTNANV VDDVIDFMLG RFRAWYQDEG YTVDTIQAVL
560 570 580 590 600
ARRPTRPADF DARMKAVSHF RTLDAAAALA AANKRVSNIL AKSDEVLSDR
610 620 630 640 650
VNASTLKEPE EIKLAMQVVV LRDKLEPYFT EGRYQDALVE LAELREPVDA
660 670 680
FFDKVMVMVD DKELRINRLT MLEKLRELFL RVADISLLQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | S → Q in AAA23915 (PubMed:6309809).Curated | 1 | |
Sequence conflicti | 91 | A → P in AAA23915 (PubMed:6309809).Curated | 1 | |
Sequence conflicti | 154 – 156 | DVH → AAA in AAA23915 (PubMed:6309809).Curated | 3 | |
Sequence conflicti | 211 – 212 | ER → DG in AAA23915 (PubMed:6309809).Curated | 2 | |
Sequence conflicti | 342 | L → D in AAA23915 (PubMed:6309809).Curated | 1 | |
Sequence conflicti | 361 | L → V in AAA23915 (PubMed:6309809).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01622 Genomic DNA Translation: AAA23915.1 U00039 Genomic DNA Translation: AAB18536.1 U00096 Genomic DNA Translation: AAC76583.1 AP009048 Genomic DNA Translation: BAE77735.1 J01623 Genomic DNA Translation: AAA23917.1 |
PIRi | S47780, SYECGB |
RefSeqi | NP_418016.1, NC_000913.3 WP_001291772.1, NZ_SSZK01000041.1 |
Genome annotation databases
EnsemblBacteriai | AAC76583; AAC76583; b3559 BAE77735; BAE77735; BAE77735 |
GeneIDi | 948080 |
KEGGi | ecj:JW3530 eco:b3559 |
PATRICi | fig|1411691.4.peg.3154 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01622 Genomic DNA Translation: AAA23915.1 U00039 Genomic DNA Translation: AAB18536.1 U00096 Genomic DNA Translation: AAC76583.1 AP009048 Genomic DNA Translation: BAE77735.1 J01623 Genomic DNA Translation: AAA23917.1 |
PIRi | S47780, SYECGB |
RefSeqi | NP_418016.1, NC_000913.3 WP_001291772.1, NZ_SSZK01000041.1 |
3D structure databases
SMRi | P00961 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259716, 23 interactors 852388, 1 interactor |
ComplexPortali | CPX-5201, Glycyl-tRNA synthetase complex |
DIPi | DIP-6880N |
IntActi | P00961, 11 interactors |
STRINGi | 511145.b3559 |
Proteomic databases
jPOSTi | P00961 |
PaxDbi | P00961 |
PRIDEi | P00961 |
Genome annotation databases
EnsemblBacteriai | AAC76583; AAC76583; b3559 BAE77735; BAE77735; BAE77735 |
GeneIDi | 948080 |
KEGGi | ecj:JW3530 eco:b3559 |
PATRICi | fig|1411691.4.peg.3154 |
Organism-specific databases
EchoBASEi | EB0405 |
Phylogenomic databases
eggNOGi | COG0751, Bacteria |
HOGENOMi | CLU_007220_2_2_6 |
InParanoidi | P00961 |
PhylomeDBi | P00961 |
Enzyme and pathway databases
BioCyci | EcoCyc:GLYS-MONOMER MetaCyc:GLYS-MONOMER |
Miscellaneous databases
PROi | PR:P00961 |
Family and domain databases
HAMAPi | MF_00255, Gly_tRNA_synth_beta, 1 hit |
InterProi | View protein in InterPro IPR008909, DALR_anticod-bd IPR015944, Gly-tRNA-synth_bsu IPR006194, Gly-tRNA-synth_heterodimer |
PANTHERi | PTHR30075, PTHR30075, 1 hit |
Pfami | View protein in Pfam PF05746, DALR_1, 1 hit PF02092, tRNA_synt_2f, 1 hit |
PRINTSi | PR01045, TRNASYNTHGB |
TIGRFAMsi | TIGR00211, glyS, 1 hit |
PROSITEi | View protein in PROSITE PS50861, AA_TRNA_LIGASE_II_GLYAB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYGB_ECOLI | |
Accessioni | P00961Primary (citable) accession number: P00961 Secondary accession number(s): Q2M7M1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 181 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families