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Entry version 179 (12 Aug 2020)
Sequence version 4 (23 Jan 2007)
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Protein

Glycine--tRNA ligase beta subunit

Gene

glyS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:GLYS-MONOMER
ECOL316407:JW3530-MONOMER
MetaCyc:GLYS-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Glycine--tRNA ligase beta subunit (EC:6.1.1.14)
Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name:
GlyRS
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:glyS
Synonyms:glyS(B)
Ordered Locus Names:b3559, JW3530
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemoved1 Publication
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000728992 – 689Glycine--tRNA ligase beta subunitAdd BLAST688

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P00961

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P00961

PRoteomics IDEntifications database

More...
PRIDEi
P00961

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Tetramer of two alpha and two beta subunits.

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4259716, 23 interactors
852388, 1 interactor

Database of interacting proteins

More...
DIPi
DIP-6880N

Protein interaction database and analysis system

More...
IntActi
P00961, 11 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b3559

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P00961

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0751, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_007220_2_2_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P00961

KEGG Orthology (KO)

More...
KOi
K01879

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P00961

Family and domain databases

HAMAP database of protein families

More...
HAMAPi
MF_00255, Gly_tRNA_synth_beta, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR008909, DALR_anticod-bd
IPR015944, Gly-tRNA-synth_bsu
IPR006194, Gly-tRNA-synth_heterodimer

The PANTHER Classification System

More...
PANTHERi
PTHR30075, PTHR30075, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF05746, DALR_1, 1 hit
PF02092, tRNA_synt_2f, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01045, TRNASYNTHGB

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00211, glyS, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50861, AA_TRNA_LIGASE_II_GLYAB, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P00961-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEKTFLVEI GTEELPPKAL RSLAESFAAN FTAELDNAGL AHGTVQWFAA
60 70 80 90 100
PRRLALKVAN LAEAQPDREI EKRGPAIAQA FDAEGKPSKA AEGWARGCGI
110 120 130 140 150
TVDQAERLTT DKGEWLLYRA HVKGESTEAL LPNMVATSLA KLPIPKLMRW
160 170 180 190 200
GASDVHFVRP VHTVTLLLGD KVIPATILGI QSDRVIRGHR FMGEPEFTID
210 220 230 240 250
NADQYPEILR ERGKVIADYE ERKAKIKADA EEAARKIGGN ADLSESLLEE
260 270 280 290 300
VASLVEWPVV LTAKFEEKFL AVPAEALVYT MKGDQKYFPV YANDGKLLPN
310 320 330 340 350
FIFVANIESK DPQQIISGNE KVVRPRLADA EFFFNTDRKK RLEDNLPRLQ
360 370 380 390 400
TVLFQQQLGT LRDKTDRIQA LAGWIAEQIG ADVNHATRAG LLSKCDLMTN
410 420 430 440 450
MVFEFTDTQG VMGMHYARHD GEAEDVAVAL NEQYQPRFAG DDLPSNPVAC
460 470 480 490 500
ALAIADKMDT LAGIFGIGQH PKGDKDPFAL RRAALGVLRI IVEKNLNLDL
510 520 530 540 550
QTLTEEAVRL YGDKLTNANV VDDVIDFMLG RFRAWYQDEG YTVDTIQAVL
560 570 580 590 600
ARRPTRPADF DARMKAVSHF RTLDAAAALA AANKRVSNIL AKSDEVLSDR
610 620 630 640 650
VNASTLKEPE EIKLAMQVVV LRDKLEPYFT EGRYQDALVE LAELREPVDA
660 670 680
FFDKVMVMVD DKELRINRLT MLEKLRELFL RVADISLLQ
Length:689
Mass (Da):76,813
Last modified:January 23, 2007 - v4
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF123D53B25BAE23A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti2S → Q in AAA23915 (PubMed:6309809).Curated1
Sequence conflicti91A → P in AAA23915 (PubMed:6309809).Curated1
Sequence conflicti154 – 156DVH → AAA in AAA23915 (PubMed:6309809).Curated3
Sequence conflicti211 – 212ER → DG in AAA23915 (PubMed:6309809).Curated2
Sequence conflicti342L → D in AAA23915 (PubMed:6309809).Curated1
Sequence conflicti361L → V in AAA23915 (PubMed:6309809).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
J01622 Genomic DNA Translation: AAA23915.1
U00039 Genomic DNA Translation: AAB18536.1
U00096 Genomic DNA Translation: AAC76583.1
AP009048 Genomic DNA Translation: BAE77735.1
J01623 Genomic DNA Translation: AAA23917.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S47780, SYECGB

NCBI Reference Sequences

More...
RefSeqi
NP_418016.1, NC_000913.3
WP_001291772.1, NZ_SSZK01000041.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC76583; AAC76583; b3559
BAE77735; BAE77735; BAE77735

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
948080

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW3530
eco:b3559

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.3154

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01622 Genomic DNA Translation: AAA23915.1
U00039 Genomic DNA Translation: AAB18536.1
U00096 Genomic DNA Translation: AAC76583.1
AP009048 Genomic DNA Translation: BAE77735.1
J01623 Genomic DNA Translation: AAA23917.1
PIRiS47780, SYECGB
RefSeqiNP_418016.1, NC_000913.3
WP_001291772.1, NZ_SSZK01000041.1

3D structure databases

SMRiP00961
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4259716, 23 interactors
852388, 1 interactor
DIPiDIP-6880N
IntActiP00961, 11 interactors
STRINGi511145.b3559

Proteomic databases

jPOSTiP00961
PaxDbiP00961
PRIDEiP00961

Genome annotation databases

EnsemblBacteriaiAAC76583; AAC76583; b3559
BAE77735; BAE77735; BAE77735
GeneIDi948080
KEGGiecj:JW3530
eco:b3559
PATRICifig|1411691.4.peg.3154

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0405

Phylogenomic databases

eggNOGiCOG0751, Bacteria
HOGENOMiCLU_007220_2_2_6
InParanoidiP00961
KOiK01879
PhylomeDBiP00961

Enzyme and pathway databases

BioCyciEcoCyc:GLYS-MONOMER
ECOL316407:JW3530-MONOMER
MetaCyc:GLYS-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P00961

Family and domain databases

HAMAPiMF_00255, Gly_tRNA_synth_beta, 1 hit
InterProiView protein in InterPro
IPR008909, DALR_anticod-bd
IPR015944, Gly-tRNA-synth_bsu
IPR006194, Gly-tRNA-synth_heterodimer
PANTHERiPTHR30075, PTHR30075, 1 hit
PfamiView protein in Pfam
PF05746, DALR_1, 1 hit
PF02092, tRNA_synt_2f, 1 hit
PRINTSiPR01045, TRNASYNTHGB
TIGRFAMsiTIGR00211, glyS, 1 hit
PROSITEiView protein in PROSITE
PS50861, AA_TRNA_LIGASE_II_GLYAB, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYGB_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P00961
Secondary accession number(s): Q2M7M1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: August 12, 2020
This is version 179 of the entry and version 4 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
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