UniProtKB - P00959 (SYM_ECOLI)
Protein
Methionine--tRNA ligase
Gene
metG
Organism
Escherichia coli (strain K12)
Status
Functioni
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
Catalytic activityi
- EC:6.1.1.10
Cofactori
Zn2+Note: Binds 1 zinc ion per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 146 | Zinc | 1 | |
Metal bindingi | 149 | Zinc | 1 | |
Metal bindingi | 159 | Zinc | 1 | |
Metal bindingi | 162 | Zinc | 1 | |
Binding sitei | 336 | ATP | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- methionine-tRNA ligase activity Source: EcoCyc
- tRNA binding Source: UniProtKB-KW
- zinc ion binding Source: EcoCyc
GO - Biological processi
- methionyl-tRNA aminoacylation Source: EcoCyc
- tRNA aminoacylation for protein translation Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:METG-MONOMER MetaCyc:METG-MONOMER |
BRENDAi | 6.1.1.10, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Methionine--tRNA ligase (EC:6.1.1.10)Alternative name(s): Methionyl-tRNA synthetase Short name: MetRS |
Gene namesi | Name:metG Ordered Locus Names:b2114, JW2101 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 336 | K → Q, A, E or R: Loss of activity. 2 Publications | 1 |
Chemistry databases
ChEMBLi | CHEMBL3367 |
DrugBanki | DB02229, 5'-O-[(L-methionyl)-sulphamoyl]adenosine DB03816, Difluoromethionine DB04015, Methionine Phosphinate DB02151, Methionine Phosphonate DB03799, Trifluoromethionine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000139129 | 2 – 677 | Methionine--tRNA ligaseAdd BLAST | 676 |
Proteomic databases
jPOSTi | P00959 |
PaxDbi | P00959 |
PRIDEi | P00959 |
2D gel databases
SWISS-2DPAGEi | P00959 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
P00959
With | #Exp. | IntAct |
---|---|---|
rplB [P60422] | 2 | EBI-909268,EBI-543515 |
Protein-protein interaction databases
BioGRIDi | 4259174, 16 interactors 850988, 1 interactor |
DIPi | DIP-10194N |
IntActi | P00959, 4 interactors |
STRINGi | 511145.b2114 |
Chemistry databases
BindingDBi | P00959 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00959 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00959 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 575 – 677 | tRNA-bindingAdd BLAST | 103 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 15 – 25 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 333 – 337 | 'KMSKS' region | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0073, Bacteria COG0143, Bacteria |
HOGENOMi | CLU_009710_7_0_6 |
InParanoidi | P00959 |
PhylomeDBi | P00959 |
Family and domain databases
CDDi | cd07957, Anticodon_Ia_Met, 1 hit cd00814, MetRS_core, 1 hit cd02800, tRNA_bind_EcMetRS_like, 1 hit |
Gene3Di | 2.20.28.20, 1 hit 3.40.50.620, 1 hit |
HAMAPi | MF_00098, Met_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR041872, Anticodon_Met IPR004495, Met-tRNA-synth_bsu_C IPR023458, Met-tRNA_ligase_1 IPR014758, Met-tRNA_synth IPR015413, Methionyl/Leucyl_tRNA_Synth IPR033911, MetRS_core IPR029038, MetRS_Zn IPR012340, NA-bd_OB-fold IPR014729, Rossmann-like_a/b/a_fold IPR002547, tRNA-bd_dom IPR009080, tRNAsynth_Ia_anticodon-bd |
Pfami | View protein in Pfam PF09334, tRNA-synt_1g, 1 hit PF01588, tRNA_bind, 1 hit |
PRINTSi | PR01041, TRNASYNTHMET |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50249, SSF50249, 1 hit SSF57770, SSF57770, 1 hit |
TIGRFAMsi | TIGR00398, metG, 1 hit TIGR00399, metG_C_term, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS50886, TRBD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00959-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC
60 70 80 90 100
ADDAHGTPIM LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS
110 120 130 140 150
EENRQLSELI YSRLKENGFI KNRTISQLYD PEKGMFLPDR FVKGTCPKCK
160 170 180 190 200
SPDQYGDNCE VCGATYSPTE LIEPKSVVSG ATPVMRDSEH FFFDLPSFSE
210 220 230 240 250
MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG FEIPNAPGKY
260 270 280 290 300
FYVWLDAPIG YMGSFKNLCD KRGDSVSFDE YWKKDSTAEL YHFIGKDIVY
310 320 330 340 350
FHSLFWPAML EGSNFRKPSN LFVHGYVTVN GAKMSKSRGT FIKASTWLNH
360 370 380 390 400
FDADSLRYYY TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF
410 420 430 440 450
INKRFDGVLA SELADPQLYK TFTDAAEVIG EAWESREFGK AVREIMALAD
460 470 480 490 500
LANRYVDEQA PWVVAKQEGR DADLQAICSM GINLFRVLMT YLKPVLPKLT
510 520 530 540 550
ERAEAFLNTE LTWDGIQQPL LGHKVNPFKA LYNRIDMRQV EALVEASKEE
560 570 580 590 600
VKAAAAPVTG PLADDPIQET ITFDDFAKVD LRVALIENAE FVEGSDKLLR
610 620 630 640 650
LTLDLGGEKR NVFSGIRSAY PDPQALIGRH TIMVANLAPR KMRFGISEGM
660 670
VMAAGPGGKD IFLLSPDAGA KPGHQVK
Sequence cautioni
The sequence AAA60526 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02671 Genomic DNA Translation: AAA24161.1 X55791 Genomic DNA Translation: CAA39315.1 U00007 Genomic DNA Translation: AAA60526.1 Different initiation. U00096 Genomic DNA Translation: AAC75175.1 AP009048 Genomic DNA Translation: BAE76592.1 |
PIRi | S14427, SYECMT |
RefSeqi | NP_416617.1, NC_000913.3 WP_001350533.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75175; AAC75175; b2114 BAE76592; BAE76592; BAE76592 |
GeneIDi | 946643 |
KEGGi | ecj:JW2101 eco:b2114 |
PATRICi | fig|1411691.4.peg.133 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02671 Genomic DNA Translation: AAA24161.1 X55791 Genomic DNA Translation: CAA39315.1 U00007 Genomic DNA Translation: AAA60526.1 Different initiation. U00096 Genomic DNA Translation: AAC75175.1 AP009048 Genomic DNA Translation: BAE76592.1 |
PIRi | S14427, SYECMT |
RefSeqi | NP_416617.1, NC_000913.3 WP_001350533.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F4L | X-ray | 1.85 | A | 1-551 | [»] | |
1MEA | NMR | - | A | 139-164 | [»] | |
1MED | NMR | - | A | 139-164 | [»] | |
1P7P | X-ray | 1.80 | A | 2-552 | [»] | |
1PFU | X-ray | 1.91 | A | 2-552 | [»] | |
1PFV | X-ray | 1.70 | A | 2-552 | [»] | |
1PFW | X-ray | 1.78 | A | 2-552 | [»] | |
1PFY | X-ray | 1.93 | A | 2-552 | [»] | |
1PG0 | X-ray | 1.90 | A | 2-552 | [»] | |
1PG2 | X-ray | 1.75 | A | 2-552 | [»] | |
1QQT | X-ray | 2.03 | A | 2-552 | [»] | |
3H97 | X-ray | 1.70 | A | 1-548 | [»] | |
3H99 | X-ray | 1.40 | A | 1-548 | [»] | |
3H9B | X-ray | 1.50 | A | 1-548 | [»] | |
3H9C | X-ray | 1.40 | A | 2-548 | [»] | |
6SPN | X-ray | 1.45 | A | 2-548 | [»] | |
6SPO | X-ray | 1.20 | A | 2-548 | [»] | |
6SPP | X-ray | 1.49 | A | 2-548 | [»] | |
6SPQ | X-ray | 1.38 | A | 2-548 | [»] | |
6SPR | X-ray | 1.48 | A | 2-548 | [»] | |
SMRi | P00959 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259174, 16 interactors 850988, 1 interactor |
DIPi | DIP-10194N |
IntActi | P00959, 4 interactors |
STRINGi | 511145.b2114 |
Chemistry databases
BindingDBi | P00959 |
ChEMBLi | CHEMBL3367 |
DrugBanki | DB02229, 5'-O-[(L-methionyl)-sulphamoyl]adenosine DB03816, Difluoromethionine DB04015, Methionine Phosphinate DB02151, Methionine Phosphonate DB03799, Trifluoromethionine |
2D gel databases
SWISS-2DPAGEi | P00959 |
Proteomic databases
jPOSTi | P00959 |
PaxDbi | P00959 |
PRIDEi | P00959 |
Genome annotation databases
EnsemblBacteriai | AAC75175; AAC75175; b2114 BAE76592; BAE76592; BAE76592 |
GeneIDi | 946643 |
KEGGi | ecj:JW2101 eco:b2114 |
PATRICi | fig|1411691.4.peg.133 |
Organism-specific databases
EchoBASEi | EB0581 |
Phylogenomic databases
eggNOGi | COG0073, Bacteria COG0143, Bacteria |
HOGENOMi | CLU_009710_7_0_6 |
InParanoidi | P00959 |
PhylomeDBi | P00959 |
Enzyme and pathway databases
BioCyci | EcoCyc:METG-MONOMER MetaCyc:METG-MONOMER |
BRENDAi | 6.1.1.10, 2026 |
Miscellaneous databases
EvolutionaryTracei | P00959 |
PROi | PR:P00959 |
Family and domain databases
CDDi | cd07957, Anticodon_Ia_Met, 1 hit cd00814, MetRS_core, 1 hit cd02800, tRNA_bind_EcMetRS_like, 1 hit |
Gene3Di | 2.20.28.20, 1 hit 3.40.50.620, 1 hit |
HAMAPi | MF_00098, Met_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR041872, Anticodon_Met IPR004495, Met-tRNA-synth_bsu_C IPR023458, Met-tRNA_ligase_1 IPR014758, Met-tRNA_synth IPR015413, Methionyl/Leucyl_tRNA_Synth IPR033911, MetRS_core IPR029038, MetRS_Zn IPR012340, NA-bd_OB-fold IPR014729, Rossmann-like_a/b/a_fold IPR002547, tRNA-bd_dom IPR009080, tRNAsynth_Ia_anticodon-bd |
Pfami | View protein in Pfam PF09334, tRNA-synt_1g, 1 hit PF01588, tRNA_bind, 1 hit |
PRINTSi | PR01041, TRNASYNTHMET |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50249, SSF50249, 1 hit SSF57770, SSF57770, 1 hit |
TIGRFAMsi | TIGR00398, metG, 1 hit TIGR00399, metG_C_term, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS50886, TRBD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYM_ECOLI | |
Accessioni | P00959Primary (citable) accession number: P00959 Secondary accession number(s): Q2MAW4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 214 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families