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UniProtKB - P00956 (SYI_ECOLI)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Miscellaneous

Strain PS102 is resistant to the antibiotic mupirocin (pseudomonic acid A), an Ile-analog that competitively inhibits activation by Ile-tRNA synthetase, thus inhibiting protein biosynthesis.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=5 µM for isoleucine1 Publication
  2. KM=0.4 mM for ATP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei561Aminoacyl-adenylateBy similarity1
    Binding sitei605ATPBy similarity1
    <p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi901ZincBy similarity1
    Metal bindingi904ZincBy similarity1
    Metal bindingi921ZincBy similarity1
    Metal bindingi924ZincBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    View the complete GO annotation on QuickGO ...

    GO - Biological processi

    View the complete GO annotation on QuickGO ...

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionAminoacyl-tRNA synthetase, Ligase
    Biological processAntibiotic resistance, Protein biosynthesis
    LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...    BioCyci    		EcoCyc:ILES-MONOMER
    MetaCyc:ILES-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:ileS
    Synonyms:ilvS
    Ordered Locus Names:b0026, JW0024
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    Escherichia coli strain K12 genome database

    More...    EcoGenei    		EG10492 ileS

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    GO - Cellular componenti

    View the complete GO annotation on QuickGO ...

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi94G → R: 6000-fold increase in Km for isoleucine and 4-fold increase in Km for ATP, with no effect on activity. 1 Publication1
    Mutagenesisi97C → S: No effect on activity. 1 Publication1
    Mutagenesisi102I → N: No significant effect on activity. 1 Publication1
    Mutagenesisi183K → A: Abolishes translocation from the aminoacylation site to the editing site, without effect on aminoacylation activity and posttransfer editing; when associated with A-421. 1 Publication1
    Mutagenesisi241T → A: Nearly the same editing activity as the wild-type. 1 Publication1
    Mutagenesisi242T → A: Abolishes editing activity against valine, with little change in aminoacylation activity; when associated with A-250. 1 Publication1
    Mutagenesisi242T → P: Abolishes editing activity against valine, with little change in aminoacylation activity. 1 Publication1
    Mutagenesisi243T → A: Abolishes editing activity against valine, with little change in aminoacylation activity. 2 Publications1
    Mutagenesisi243T → R: Abolishes pretransfer editing. 2 Publications1
    Mutagenesisi246T → A: Nearly the same editing activity as the wild-type. 1 Publication1
    Mutagenesisi250N → A: Abolishes editing activity against valine, with little change in aminoacylation activity. 2 Publications1
    Mutagenesisi333H → A: Alters the specificity for hydrolysis of the aminoacyl tRNA ester, with no effect on pretransfer editing. 1 Publication1
    Mutagenesisi342D → A or N: Strong decrease in total editing and deacylation activities. Severely deficient in translocation from the aminoacylation site to the editing site. 1 Publication1
    Mutagenesisi342D → E: Reduces 2- to 3-fold the total editing activity and 2-fold the deacylation activity. Moderately reduces translocation from the aminoacylation site to the editing site. 1 Publication1
    Mutagenesisi421W → A: Abolishes translocation from the aminoacylation site to the editing site, without effect on aminoacylation activity and posttransfer editing; when associated with A-183. 1 Publication1

    Chemistry databases

    ChEMBL database of bioactive drug-like small molecules

    More...    ChEMBLi    		CHEMBL3657

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemoved1 Publication
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000983842 – 938Isoleucine--tRNA ligaseAdd BLAST937

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei183N6-acetyllysine1 Publication1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    Encyclopedia of Proteome Dynamics

    More...    EPDi    		P00956

    jPOST - Japan Proteome Standard Repository/Database

    More...    jPOSTi    		P00956

    PaxDb, a database of protein abundance averages across all three domains of life

    More...    PaxDbi    		P00956

    PRoteomics IDEntifications database

    More...    PRIDEi    		P00956

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...    iPTMneti    		P00956

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Monomer.

    <p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGrid)

    More...    BioGridi    		4261375, 35 interactors
    849163, 1 interactor

    Database of interacting proteins

    More...    DIPi    		DIP-10017N

    Protein interaction database and analysis system

    More...    IntActi    		P00956, 17 interactors

    STRING: functional protein association networks

    More...    STRINGi    		316385.ECDH10B_0027

    Chemistry databases

    BindingDB database of measured binding affinities

    More...    BindingDBi    		P00956

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

    More...    ProteinModelPortali    		P00956

    Database of comparative protein structure models

    More...    ModBasei    		Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...    MobiDBi    		Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi58 – 68"HIGH" regionAdd BLAST11
    Motifi602 – 606"KMSKS" region5

    <p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...    eggNOGi    		ENOG4105C07 Bacteria
    COG0060 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...    HOGENOMi    		HOG000246402

    InParanoid: Eukaryotic Ortholog Groups

    More...    InParanoidi    		P00956

    KEGG Orthology (KO)

    More...    KOi    		K01870

    Database for complete collections of gene phylogenies

    More...    PhylomeDBi    		P00956

    Family and domain databases

    Conserved Domains Database

    More...    CDDi    		cd07960 Anticodon_Ia_Ile_BEm, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...    Gene3Di    		3.40.50.620, 1 hit
    3.90.740.10, 1 hit

    HAMAP database of protein families

    More...    HAMAPi    		MF_02002 Ile_tRNA_synth_type1, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...    InterProi    		View protein in InterPro
    IPR001412 aa-tRNA-synth_I_CS
    IPR002300 aa-tRNA-synth_Ia
    IPR033708 Anticodon_Ile_BEm
    IPR002301 Ile-tRNA-ligase
    IPR023585 Ile-tRNA-ligase_type1
    IPR013155 M/V/L/I-tRNA-synth_anticd-bd
    IPR014729 Rossmann-like_a/b/a_fold
    IPR009080 tRNAsynth_Ia_anticodon-bd
    IPR009008 Val/Leu/Ile-tRNA-synth_edit
    IPR010663 Znf_FPG/IleRS

    Pfam protein domain database

    More...    Pfami    		View protein in Pfam
    PF08264 Anticodon_1, 1 hit
    PF00133 tRNA-synt_1, 1 hit
    PF06827 zf-FPG_IleRS, 1 hit

    Protein Motif fingerprint database; a protein domain database

    More...    PRINTSi    		PR00984 TRNASYNTHILE

    Superfamily database of structural and functional annotation

    More...    SUPFAMi    		SSF47323 SSF47323, 1 hit
    SSF50677 SSF50677, 1 hit

    TIGRFAMs; a protein family database

    More...    TIGRFAMsi    		TIGR00392 ileS, 1 hit

    PROSITE; a protein domain and family database

    More...    PROSITEi    		View protein in PROSITE
    PS00178 AA_TRNA_LIGASE_I, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

    P00956-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSDYKSTLNL PETGFPMRGD LAKREPGMLA RWTDDDLYGI IRAAKKGKKT 
            60         70         80         90        100
    FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKGLSGYDSP YVPGWDCHGL 
           110        120        130        140        150
    PIELKVEQEY GKPGEKFTAA EFRAKCREYA ATQVDGQRKD FIRLGVLGDW 
           160        170        180        190        200
    SHPYLTMDFK TEANIIRALG KIIGNGHLHK GAKPVHWCVD CRSALAEAEV 
           210        220        230        240        250
    EYYDKTSPSI DVAFQAVDQD ALKAKFAVSN VNGPISLVIW TTTPWTLPAN 
           260        270        280        290        300
    RAISIAPDFD YALVQIDGQA VILAKDLVES VMQRIGVTDY TILGTVKGAE 
           310        320        330        340        350
    LELLRFTHPF MGFDVPAILG DHVTLDAGTG AVHTAPGHGP DDYVIGQKYG 
           360        370        380        390        400
    LETANPVGPD GTYLPGTYPT LDGVNVFKAN DIVVALLQEK GALLHVEKMQ 
           410        420        430        440        450
    HSYPCCWRHK TPIIFRATPQ WFVSMDQKGL RAQSLKEIKG VQWIPDWGQA 
           460        470        480        490        500
    RIESMVANRP DWCISRQRTW GVPMSLFVHK DTEELHPRTL ELMEEVAKRV 
           510        520        530        540        550
    EVDGIQAWWD LDAKEILGDE ADQYVKVPDT LDVWFDSGST HSSVVDVRPE 
           560        570        580        590        600
    FAGHAADMYL EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG 
           610        620        630        640        650
    RKMSKSIGNT VSPQDVMNKL GADILRLWVA STDYTGEMAV SDEILKRAAD 
           660        670        680        690        700
    SYRRIRNTAR FLLANLNGFD PAKDMVKPEE MVVLDRWAVG CAKAAQEDIL 
           710        720        730        740        750
    KAYEAYDFHE VVQRLMRFCS VEMGSFYLDI IKDRQYTAKA DSVARRSCQT 
           760        770        780        790        800
    ALYHIAEALV RWMAPILSFT ADEVWGYLPG EREKYVFTGE WYEGLFGLAD 
           810        820        830        840        850
    SEAMNDAFWD ELLKVRGEVN KVIEQARADK KVGGSLEAAV TLYAEPELSA 
           860        870        880        890        900
    KLTALGDELR FVLLTSGATV ADYNDAPADA QQSEVLKGLK VALSKAEGEK 
           910        920        930 
    CPRCWHYTQD VGKVAEHAEI CGRCVSNVAG DGEKRKFA
    Length:938
    Mass (Da):104,297
    Last modified:January 23, 2007 - v5
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i238CEDAF461F01D5
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti243 – 264TPWTL…DYALV → RRGLCLPTAQSLLHQISTMR WW AA sequence (PubMed:6390679).CuratedAdd BLAST22
    Sequence conflicti300 – 301EL → DV AA sequence (PubMed:6390679).Curated2
    Sequence conflicti587R → C AA sequence (PubMed:6390679).Curated1
    Sequence conflicti637E → Q AA sequence (PubMed:6390679).Curated1
    Sequence conflicti724G → V AA sequence (PubMed:6390679).Curated1
    Sequence conflicti738A → P AA sequence (PubMed:6390679).Curated1
    Sequence conflicti740 – 743ADSV → RTVW AA sequence (PubMed:6390679).Curated4
    Sequence conflicti787F → L AA sequence (PubMed:6390679).Curated1
    Sequence conflicti830K → N AA sequence (PubMed:6390679).Curated1
    Sequence conflicti867 – 869GAT → DRRY AA sequence (PubMed:6390679).Curated3

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural varianti594F → L in strain: PS102. 1

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...    EMBLi

    GenBank nucleotide sequence database

    More...    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...    DDBJi
    Links Updated
    		U00096 Genomic DNA Translation: AAC73137.1
    AP009048 Genomic DNA Translation: BAB96595.2
    M10428 Genomic DNA Translation: AAA24606.1
    X00776 Genomic DNA Translation: CAA25352.1
    K01990 Genomic DNA Translation: AAA24091.1

    Protein sequence database of the Protein Information Resource

    More...    PIRi    		B64723 SYECIT

    NCBI Reference Sequences

    More...    RefSeqi    		NP_414567.1, NC_000913.3
    WP_001286857.1, NZ_LN832404.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...    EnsemblBacteriai    		AAC73137; AAC73137; b0026
    BAB96595; BAB96595; BAB96595

    Database of genes from NCBI RefSeq genomes

    More...    GeneIDi    		944761

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...    KEGGi    		ecj:JW0024
    eco:b0026

    Pathosystems Resource Integration Center (PATRIC)

    More...    PATRICi    		fig|1411691.4.peg.2259

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		U00096 Genomic DNA Translation: AAC73137.1
    AP009048 Genomic DNA Translation: BAB96595.2
    M10428 Genomic DNA Translation: AAA24606.1
    X00776 Genomic DNA Translation: CAA25352.1
    K01990 Genomic DNA Translation: AAA24091.1
    PIRiB64723 SYECIT
    RefSeqiNP_414567.1, NC_000913.3
    WP_001286857.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP00956
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261375, 35 interactors
    849163, 1 interactor
    DIPiDIP-10017N
    IntActiP00956, 17 interactors
    STRINGi316385.ECDH10B_0027

    Chemistry databases

    BindingDBiP00956
    ChEMBLiCHEMBL3657

    PTM databases

    iPTMnetiP00956

    Proteomic databases

    EPDiP00956
    jPOSTiP00956
    PaxDbiP00956
    PRIDEiP00956

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73137; AAC73137; b0026
    BAB96595; BAB96595; BAB96595
    GeneIDi944761
    KEGGiecj:JW0024
    eco:b0026
    PATRICifig|1411691.4.peg.2259

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...    EchoBASEi    		EB0487
    EcoGeneiEG10492 ileS

    Phylogenomic databases

    eggNOGiENOG4105C07 Bacteria
    COG0060 LUCA
    HOGENOMiHOG000246402
    InParanoidiP00956
    KOiK01870
    PhylomeDBiP00956

    Enzyme and pathway databases

    BioCyciEcoCyc:ILES-MONOMER
    MetaCyc:ILES-MONOMER

    Miscellaneous databases

    Protein Ontology

    More...    PROi    		PR:P00956

    Family and domain databases

    CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
    Gene3Di3.40.50.620, 1 hit
    3.90.740.10, 1 hit
    HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
    InterProiView protein in InterPro
    IPR001412 aa-tRNA-synth_I_CS
    IPR002300 aa-tRNA-synth_Ia
    IPR033708 Anticodon_Ile_BEm
    IPR002301 Ile-tRNA-ligase
    IPR023585 Ile-tRNA-ligase_type1
    IPR013155 M/V/L/I-tRNA-synth_anticd-bd
    IPR014729 Rossmann-like_a/b/a_fold
    IPR009080 tRNAsynth_Ia_anticodon-bd
    IPR009008 Val/Leu/Ile-tRNA-synth_edit
    IPR010663 Znf_FPG/IleRS
    PfamiView protein in Pfam
    PF08264 Anticodon_1, 1 hit
    PF00133 tRNA-synt_1, 1 hit
    PF06827 zf-FPG_IleRS, 1 hit
    PRINTSiPR00984 TRNASYNTHILE
    SUPFAMiSSF47323 SSF47323, 1 hit
    SSF50677 SSF50677, 1 hit
    TIGRFAMsiTIGR00392 ileS, 1 hit
    PROSITEiView protein in PROSITE
    PS00178 AA_TRNA_LIGASE_I, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...    ProtoNeti    		Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYI_ECOLI
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P00956
    Secondary accession number(s): P78038, Q59429
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: January 16, 2019
    This is version 186 of the entry and version 5 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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