UniProtKB - P00956 (SYI_ECOLI)
Isoleucine--tRNA ligase
ileS
Functioni
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).
8 PublicationsMiscellaneous
Catalytic activityi
- EC:6.1.1.51 Publication
Cofactori
Kineticsi
- KM=5 µM for isoleucine1 Publication
- KM=0.4 mM for ATP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 561 | Isoleucyl-adenylateBy similarity | 1 | |
Binding sitei | 602 | ATP1 Publication | 1 | |
Binding sitei | 605 | ATP1 Publication | 1 | |
Metal bindingi | 901 | ZincBy similarity | 1 | |
Metal bindingi | 904 | ZincBy similarity | 1 | |
Metal bindingi | 921 | ZincBy similarity | 1 | |
Metal bindingi | 924 | ZincBy similarity | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: InterPro
- ATP binding Source: UniProtKB-UniRule
- isoleucine-tRNA ligase activity Source: GO_Central
- tRNA binding Source: InterPro
- zinc ion binding Source: EcoCyc
GO - Biological processi
- isoleucyl-tRNA aminoacylation Source: EcoCyc
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Antibiotic resistance, Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:ILES-MONOMER |
BRENDAi | 6.1.1.5, 2026 |
SABIO-RKi | P00956 |
Names & Taxonomyi
Protein namesi | Recommended name: Isoleucine--tRNA ligase (EC:6.1.1.51 Publication)Alternative name(s): Isoleucyl-tRNA synthetase Short name: IleRS |
Gene namesi | Name:ileS Synonyms:ilvS Ordered Locus Names:b0026, JW0024 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 94 | G → R: 6000-fold increase in Km for isoleucine and 4-fold increase in Km for ATP, with no effect on activity. 1 Publication | 1 | |
Mutagenesisi | 97 | C → S: No effect on activity. 1 Publication | 1 | |
Mutagenesisi | 102 | I → N: No significant effect on activity. 1 Publication | 1 | |
Mutagenesisi | 183 | K → A: Abolishes translocation from the aminoacylation site to the editing site, without effect on aminoacylation activity and posttransfer editing; when associated with A-421. 1 Publication | 1 | |
Mutagenesisi | 241 | T → A: Nearly the same editing activity as the wild-type. 1 Publication | 1 | |
Mutagenesisi | 242 | T → A: Abolishes editing activity against valine, with little change in aminoacylation activity; when associated with A-250. 1 Publication | 1 | |
Mutagenesisi | 242 | T → P: Abolishes editing activity against valine, with little change in aminoacylation activity. 1 Publication | 1 | |
Mutagenesisi | 243 | T → A: Abolishes editing activity against valine, with little change in aminoacylation activity. 2 Publications | 1 | |
Mutagenesisi | 243 | T → R: Abolishes pretransfer editing. 2 Publications | 1 | |
Mutagenesisi | 246 | T → A: Nearly the same editing activity as the wild-type. 1 Publication | 1 | |
Mutagenesisi | 250 | N → A: Abolishes editing activity against valine, with little change in aminoacylation activity. 2 Publications | 1 | |
Mutagenesisi | 333 | H → A: Alters the specificity for hydrolysis of the aminoacyl tRNA ester, with no effect on pretransfer editing. 1 Publication | 1 | |
Mutagenesisi | 342 | D → A or N: Strong decrease in total editing and deacylation activities. Severely deficient in translocation from the aminoacylation site to the editing site. 1 Publication | 1 | |
Mutagenesisi | 342 | D → E: Reduces 2- to 3-fold the total editing activity and 2-fold the deacylation activity. Moderately reduces translocation from the aminoacylation site to the editing site. 1 Publication | 1 | |
Mutagenesisi | 421 | W → A: Abolishes translocation from the aminoacylation site to the editing site, without effect on aminoacylation activity and posttransfer editing; when associated with A-183. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3657 |
DrugCentrali | P00956 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000098384 | 2 – 938 | Isoleucine--tRNA ligaseAdd BLAST | 937 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 183 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P00956 |
PaxDbi | P00956 |
PRIDEi | P00956 |
PTM databases
iPTMneti | P00956 |
Interactioni
Subunit structurei
Monomer.
Binary interactionsi
P00956
With | #Exp. | IntAct |
---|---|---|
hyaD [P19930] | 3 | EBI-552928,EBI-552940 |
rbsB [P02925] | 3 | EBI-552928,EBI-369930 |
tufA [P0CE47] | 2 | EBI-552928,EBI-301077 |
yjgL [P39336] | 3 | EBI-552928,EBI-549937 |
Protein-protein interaction databases
BioGRIDi | 4261375, 35 interactors 849163, 6 interactors |
DIPi | DIP-10017N |
IntActi | P00956, 17 interactors |
STRINGi | 511145.b0026 |
Chemistry databases
BindingDBi | P00956 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 58 – 68 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 602 – 606 | 'KMSKS' region | 5 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
HOGENOMi | CLU_001493_7_1_6 |
InParanoidi | P00956 |
OMAi | HLGTAWN |
PhylomeDBi | P00956 |
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 2 hits 3.90.740.10, 1 hit |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR010663, Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF06827, zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSDYKSTLNL PETGFPMRGD LAKREPGMLA RWTDDDLYGI IRAAKKGKKT
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKGLSGYDSP YVPGWDCHGL
110 120 130 140 150
PIELKVEQEY GKPGEKFTAA EFRAKCREYA ATQVDGQRKD FIRLGVLGDW
160 170 180 190 200
SHPYLTMDFK TEANIIRALG KIIGNGHLHK GAKPVHWCVD CRSALAEAEV
210 220 230 240 250
EYYDKTSPSI DVAFQAVDQD ALKAKFAVSN VNGPISLVIW TTTPWTLPAN
260 270 280 290 300
RAISIAPDFD YALVQIDGQA VILAKDLVES VMQRIGVTDY TILGTVKGAE
310 320 330 340 350
LELLRFTHPF MGFDVPAILG DHVTLDAGTG AVHTAPGHGP DDYVIGQKYG
360 370 380 390 400
LETANPVGPD GTYLPGTYPT LDGVNVFKAN DIVVALLQEK GALLHVEKMQ
410 420 430 440 450
HSYPCCWRHK TPIIFRATPQ WFVSMDQKGL RAQSLKEIKG VQWIPDWGQA
460 470 480 490 500
RIESMVANRP DWCISRQRTW GVPMSLFVHK DTEELHPRTL ELMEEVAKRV
510 520 530 540 550
EVDGIQAWWD LDAKEILGDE ADQYVKVPDT LDVWFDSGST HSSVVDVRPE
560 570 580 590 600
FAGHAADMYL EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG
610 620 630 640 650
RKMSKSIGNT VSPQDVMNKL GADILRLWVA STDYTGEMAV SDEILKRAAD
660 670 680 690 700
SYRRIRNTAR FLLANLNGFD PAKDMVKPEE MVVLDRWAVG CAKAAQEDIL
710 720 730 740 750
KAYEAYDFHE VVQRLMRFCS VEMGSFYLDI IKDRQYTAKA DSVARRSCQT
760 770 780 790 800
ALYHIAEALV RWMAPILSFT ADEVWGYLPG EREKYVFTGE WYEGLFGLAD
810 820 830 840 850
SEAMNDAFWD ELLKVRGEVN KVIEQARADK KVGGSLEAAV TLYAEPELSA
860 870 880 890 900
KLTALGDELR FVLLTSGATV ADYNDAPADA QQSEVLKGLK VALSKAEGEK
910 920 930
CPRCWHYTQD VGKVAEHAEI CGRCVSNVAG DGEKRKFA
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 300 – 301 | EL → DV in strain: EM20031, MRE-600; AA sequence. 2 Publications | 2 | |
Natural varianti | 587 | R → C in strain: MRE-600; AA sequence. 1 Publication | 1 | |
Natural varianti | 594 | F → L in strain: PS102. 1 Publication | 1 | |
Natural varianti | 637 | E → Q in strain: MRE-600; AA sequence. 1 Publication | 1 | |
Natural varianti | 724 | G → V in strain: EM20031; AA sequence. 1 Publication | 1 | |
Natural varianti | 738 | A → P in strain: EM20031, MRE-600; AA sequence. 2 Publications | 1 | |
Natural varianti | 740 – 743 | ADSV → RTVW in strain: EM20031; AA sequence. 1 Publication | 4 | |
Natural varianti | 787 | F → L in strain: MRE-600; AA sequence. 1 Publication | 1 | |
Natural varianti | 830 | K → N in strain: MRE-600; AA sequence. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73137.1 AP009048 Genomic DNA Translation: BAB96595.2 M10428 Genomic DNA Translation: AAA24606.1 X00776 Genomic DNA Translation: CAA25352.1 K01990 Genomic DNA Translation: AAA24091.1 |
PIRi | B64723, SYECIT |
RefSeqi | NP_414567.1, NC_000913.3 WP_001286857.1, NZ_STEB01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC73137; AAC73137; b0026 BAB96595; BAB96595; BAB96595 |
GeneIDi | 944761 |
KEGGi | ecj:JW0024 eco:b0026 |
PATRICi | fig|1411691.4.peg.2259 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73137.1 AP009048 Genomic DNA Translation: BAB96595.2 M10428 Genomic DNA Translation: AAA24606.1 X00776 Genomic DNA Translation: CAA25352.1 K01990 Genomic DNA Translation: AAA24091.1 |
PIRi | B64723, SYECIT |
RefSeqi | NP_414567.1, NC_000913.3 WP_001286857.1, NZ_STEB01000010.1 |
3D structure databases
AlphaFoldDBi | P00956 |
SMRi | P00956 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261375, 35 interactors 849163, 6 interactors |
DIPi | DIP-10017N |
IntActi | P00956, 17 interactors |
STRINGi | 511145.b0026 |
Chemistry databases
BindingDBi | P00956 |
ChEMBLi | CHEMBL3657 |
DrugCentrali | P00956 |
PTM databases
iPTMneti | P00956 |
Proteomic databases
jPOSTi | P00956 |
PaxDbi | P00956 |
PRIDEi | P00956 |
Genome annotation databases
EnsemblBacteriai | AAC73137; AAC73137; b0026 BAB96595; BAB96595; BAB96595 |
GeneIDi | 944761 |
KEGGi | ecj:JW0024 eco:b0026 |
PATRICi | fig|1411691.4.peg.2259 |
Organism-specific databases
EchoBASEi | EB0487 |
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
HOGENOMi | CLU_001493_7_1_6 |
InParanoidi | P00956 |
OMAi | HLGTAWN |
PhylomeDBi | P00956 |
Enzyme and pathway databases
BioCyci | EcoCyc:ILES-MONOMER |
BRENDAi | 6.1.1.5, 2026 |
SABIO-RKi | P00956 |
Miscellaneous databases
PROi | PR:P00956 |
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 2 hits 3.90.740.10, 1 hit |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR010663, Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF06827, zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYI_ECOLI | |
Accessioni | P00956Primary (citable) accession number: P00956 Secondary accession number(s): P78038, Q59429 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 204 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families