UniProtKB - P00954 (SYW_ECOLI)
Protein
Tryptophan--tRNA ligase
Gene
trpS
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the attachment of tryptophan to tRNA(Trp). Amino acylates tRNA(Trp) with both L- and D-tryptophan, although D-tryptophan is a poor substrate (PubMed:10918062).UniRule annotation1 Publication
Catalytic activityi
- ATP + L-tryptophan + tRNATrp = AMP + diphosphate + H+ + L-tryptophanyl-tRNATrpUniRule annotation1 PublicationEC:6.1.1.2UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 135 | L-tryptophanUniRule annotation | 1 | |
Binding sitei | 186 | ATP; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 13 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 19 – 20 | ATPUniRule annotation | 2 | |
Nucleotide bindingi | 147 – 149 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 195 – 199 | ATPUniRule annotation | 5 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- tryptophan-tRNA ligase activity Source: EcoCyc
GO - Biological processi
- tryptophanyl-tRNA aminoacylation Source: EcoCyc
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:TRPS-MONOMER MetaCyc:TRPS-MONOMER |
SABIO-RKi | P00954 |
Names & Taxonomyi
Protein namesi | Recommended name: Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation1 Publication)Alternative name(s): Tryptophanyl-tRNA synthetaseUniRule annotation Short name: TrpRSUniRule annotation |
Gene namesi | Name:trpSUniRule annotation Ordered Locus Names:b3384, JW3347 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000136628 | 1 – 334 | Tryptophan--tRNA ligaseAdd BLAST | 334 |
Proteomic databases
jPOSTi | P00954 |
PaxDbi | P00954 |
PRIDEi | P00954 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 4259293, 179 interactors |
DIPi | DIP-11042N |
IntActi | P00954, 12 interactors |
STRINGi | 511145.b3384 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 12 – 20 | 'HIGH' regionUniRule annotation | 9 | |
Motifi | 195 – 199 | 'KMSKS' regionUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0180, Bacteria |
HOGENOMi | CLU_029244_1_1_6 |
InParanoidi | P00954 |
PhylomeDBi | P00954 |
Family and domain databases
CDDi | cd00806, TrpRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00140_B, Trp_tRNA_synth_B, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002305, aa-tRNA-synth_Ic IPR014729, Rossmann-like_a/b/a_fold IPR002306, Trp-tRNA-ligase IPR024109, Trp-tRNA-ligase_bac-type |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit |
PRINTSi | PR01039, TRNASYNTHTRP |
TIGRFAMsi | TIGR00233, trpS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
P00954-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKPIVFSGA QPSGELTIGN YMGALRQWVN MQDDYHCIYC IVDQHAITVR
60 70 80 90 100
QDAQKLRKAT LDTLALYLAC GIDPEKSTIF VQSHVPEHAQ LGWALNCYTY
110 120 130 140 150
FGELSRMTQF KDKSARYAEN INAGLFDYPV LMAADILLYQ TNLVPVGEDQ
160 170 180 190 200
KQHLELSRDI AQRFNALYGE IFKVPEPFIP KSGARVMSLL EPTKKMSKSD
210 220 230 240 250
DNRNNVIGLL EDPKSVVKKI KRAVTDSDEP PVVRYDVQNK AGVSNLLDIL
260 270 280 290 300
SAVTGQSIPE LEKQFEGKMY GHLKGEVADA VSGMLTELQE RYHRFRNDEA
310 320 330
FLQQVMKDGA EKASAHASRT LKAVYEAIGF VAKP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 30 | N → K in CAA23670 (PubMed:7042706).Curated | 1 | |
Sequence conflicti | 30 | N → K in CAA79669 (PubMed:7603433).Curated | 1 | |
Sequence conflicti | 326 | E → Q in CAA23670 (PubMed:7042706).Curated | 1 | |
Sequence conflicti | 326 | E → Q in CAA79669 (PubMed:7603433).Curated | 1 | |
Sequence conflicti | 334 | P → R in CAA23670 (PubMed:7042706).Curated | 1 | |
Sequence conflicti | 334 | P → R in CAA79669 (PubMed:7603433).Curated | 1 | |
Sequence conflicti | 334 | P → R in AAA92300 (PubMed:8555191).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 60 | T → R in TRPS567C; reduced activity, thermolabile. | 1 | |
Natural varianti | 91 | L → F in TRPS10330; reduced activity. | 1 | |
Natural varianti | 112 | D → E in TRPS9969; no effect in activity. | 1 | |
Natural varianti | 129 | P → S in TRPS9969; reduced activity. | 1 | |
Natural varianti | 133 | A → E in TRPS42C; reduced activity. | 1 | |
Natural varianti | 196 | M → I in TRPS271C; activity largely reduced. | 1 | |
Natural varianti | 329 | G → S in TRPS4040; reduced activity. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00371 Genomic DNA Translation: CAA23670.1 Z19601 Genomic DNA Translation: CAA79669.1 U38647 Genomic DNA Translation: AAA92300.1 U18997 Genomic DNA Translation: AAA58181.1 U00096 Genomic DNA Translation: AAC76409.1 AP009048 Genomic DNA Translation: BAE77907.1 |
PIRi | C65133, YWEC |
RefSeqi | NP_417843.1, NC_000913.3 WP_000165552.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76409; AAC76409; b3384 BAE77907; BAE77907; BAE77907 |
GeneIDi | 947894 |
KEGGi | ecj:JW3347 eco:b3384 |
PATRICi | fig|1411691.4.peg.3346 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00371 Genomic DNA Translation: CAA23670.1 Z19601 Genomic DNA Translation: CAA79669.1 U38647 Genomic DNA Translation: AAA92300.1 U18997 Genomic DNA Translation: AAA58181.1 U00096 Genomic DNA Translation: AAC76409.1 AP009048 Genomic DNA Translation: BAE77907.1 |
PIRi | C65133, YWEC |
RefSeqi | NP_417843.1, NC_000913.3 WP_000165552.1, NZ_SSZK01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5V0I | X-ray | 1.90 | A/B | 1-334 | [»] | |
SMRi | P00954 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259293, 179 interactors |
DIPi | DIP-11042N |
IntActi | P00954, 12 interactors |
STRINGi | 511145.b3384 |
Proteomic databases
jPOSTi | P00954 |
PaxDbi | P00954 |
PRIDEi | P00954 |
Genome annotation databases
EnsemblBacteriai | AAC76409; AAC76409; b3384 BAE77907; BAE77907; BAE77907 |
GeneIDi | 947894 |
KEGGi | ecj:JW3347 eco:b3384 |
PATRICi | fig|1411691.4.peg.3346 |
Organism-specific databases
EchoBASEi | EB1023 |
Phylogenomic databases
eggNOGi | COG0180, Bacteria |
HOGENOMi | CLU_029244_1_1_6 |
InParanoidi | P00954 |
PhylomeDBi | P00954 |
Enzyme and pathway databases
BioCyci | EcoCyc:TRPS-MONOMER MetaCyc:TRPS-MONOMER |
SABIO-RKi | P00954 |
Miscellaneous databases
PROi | PR:P00954 |
Family and domain databases
CDDi | cd00806, TrpRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00140_B, Trp_tRNA_synth_B, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002305, aa-tRNA-synth_Ic IPR014729, Rossmann-like_a/b/a_fold IPR002306, Trp-tRNA-ligase IPR024109, Trp-tRNA-ligase_bac-type |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit |
PRINTSi | PR01039, TRNASYNTHTRP |
TIGRFAMsi | TIGR00233, trpS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYW_ECOLI | |
Accessioni | P00954Primary (citable) accession number: P00954 Secondary accession number(s): Q2M749 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | August 29, 2003 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families