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Protein

Mannose-6-phosphate isomerase

Gene

manA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide.

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi97ZincBy similarity1
Metal bindingi99ZincBy similarity1
Metal bindingi134ZincBy similarity1
Metal bindingi255ZincBy similarity1
Active sitei274By similarity1

GO - Molecular functioni

  • mannose-6-phosphate isomerase activity Source: EcoCyc
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionIsomerase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:MANNPISOM-MONOMER
MetaCyc:MANNPISOM-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:manA
Synonyms:pmi
Ordered Locus Names:b1613, JW1605
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10566 manA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3309019

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001942301 – 391Mannose-6-phosphate isomeraseAdd BLAST391

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei280N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP00946
PaxDbiP00946
PRIDEiP00946

2D gel databases

SWISS-2DPAGEiP00946

PTM databases

iPTMnetiP00946

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
minCP181965EBI-554045,EBI-554060

Protein-protein interaction databases

BioGridi4260255, 312 interactors
DIPiDIP-10153N
IntActiP00946, 19 interactors
STRINGi316385.ECDH10B_1746

Structurei

3D structure databases

ProteinModelPortaliP00946
SMRiP00946
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EIP Bacteria
COG1482 LUCA
HOGENOMiHOG000241278
InParanoidiP00946
KOiK01809
OMAiMGTHPSN
PhylomeDBiP00946

Family and domain databases

Gene3Di2.60.120.10, 3 hits
InterProiView protein in InterPro
IPR001250 Man6P_Isoase-1
IPR016305 Mannose-6-P_Isomerase
IPR018050 Pmannose_isomerase-type1_CS
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR10309 PTHR10309, 1 hit
PfamiView protein in Pfam
PF01238 PMI_typeI, 1 hit
PIRSFiPIRSF001480 Mannose-6-phosphate_isomerase, 1 hit
PRINTSiPR00714 MAN6PISMRASE
SUPFAMiSSF51182 SSF51182, 1 hit
TIGRFAMsiTIGR00218 manA, 1 hit
PROSITEiView protein in PROSITE
PS00965 PMI_I_1, 1 hit
PS00966 PMI_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

P00946-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQKLINSVQN YAWGSKTALT ELYGMENPSS QPMAELWMGA HPKSSSRVQN
60 70 80 90 100
AAGDIVSLRD VIESDKSTLL GEAVAKRFGE LPFLFKVLCA AQPLSIQVHP
110 120 130 140 150
NKHNSEIGFA KENAAGIPMD AAERNYKDPN HKPELVFALT PFLAMNAFRE
160 170 180 190 200
FSEIVSLLQP VAGAHPAIAH FLQQPDAERL SELFASLLNM QGEEKSRALA
210 220 230 240 250
ILKSALDSQQ GEPWQTIRLI SEFYPEDSGL FSPLLLNVVK LNPGEAMFLF
260 270 280 290 300
AETPHAYLQG VALEVMANSD NVLRAGLTPK YIDIPELVAN VKFEAKPANQ
310 320 330 340 350
LLTQPVKQGA ELDFPIPVDD FAFSLHDLSD KETTISQQSA AILFCVEGDA
360 370 380 390
TLWKGSQQLQ LKPGESAFIA ANESPVTVKG HGRLARVYNK L
Length:391
Mass (Da):42,850
Last modified:July 21, 1986 - v1
Checksum:i669737512CADBCCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15380 Genomic DNA Translation: AAA24109.1
U00096 Genomic DNA Translation: AAC74685.1
AP009048 Genomic DNA Translation: BAA15361.1
U35067 Genomic DNA Translation: AAA79050.1
PIRiA01172 ISECMP
RefSeqiNP_416130.3, NC_000913.3
WP_001170664.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74685; AAC74685; b1613
BAA15361; BAA15361; BAA15361
GeneIDi944840
KEGGiecj:JW1605
eco:b1613
PATRICifig|1411691.4.peg.649

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15380 Genomic DNA Translation: AAA24109.1
U00096 Genomic DNA Translation: AAC74685.1
AP009048 Genomic DNA Translation: BAA15361.1
U35067 Genomic DNA Translation: AAA79050.1
PIRiA01172 ISECMP
RefSeqiNP_416130.3, NC_000913.3
WP_001170664.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP00946
SMRiP00946
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260255, 312 interactors
DIPiDIP-10153N
IntActiP00946, 19 interactors
STRINGi316385.ECDH10B_1746

Chemistry databases

ChEMBLiCHEMBL3309019

PTM databases

iPTMnetiP00946

2D gel databases

SWISS-2DPAGEiP00946

Proteomic databases

EPDiP00946
PaxDbiP00946
PRIDEiP00946

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74685; AAC74685; b1613
BAA15361; BAA15361; BAA15361
GeneIDi944840
KEGGiecj:JW1605
eco:b1613
PATRICifig|1411691.4.peg.649

Organism-specific databases

EchoBASEiEB0561
EcoGeneiEG10566 manA

Phylogenomic databases

eggNOGiENOG4105EIP Bacteria
COG1482 LUCA
HOGENOMiHOG000241278
InParanoidiP00946
KOiK01809
OMAiMGTHPSN
PhylomeDBiP00946

Enzyme and pathway databases

BioCyciEcoCyc:MANNPISOM-MONOMER
MetaCyc:MANNPISOM-MONOMER

Miscellaneous databases

PROiPR:P00946

Family and domain databases

Gene3Di2.60.120.10, 3 hits
InterProiView protein in InterPro
IPR001250 Man6P_Isoase-1
IPR016305 Mannose-6-P_Isomerase
IPR018050 Pmannose_isomerase-type1_CS
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR10309 PTHR10309, 1 hit
PfamiView protein in Pfam
PF01238 PMI_typeI, 1 hit
PIRSFiPIRSF001480 Mannose-6-phosphate_isomerase, 1 hit
PRINTSiPR00714 MAN6PISMRASE
SUPFAMiSSF51182 SSF51182, 1 hit
TIGRFAMsiTIGR00218 manA, 1 hit
PROSITEiView protein in PROSITE
PS00965 PMI_I_1, 1 hit
PS00966 PMI_I_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMANA_ECOLI
AccessioniPrimary (citable) accession number: P00946
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 18, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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