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UniProtKB - P00942 (TPIS_YEAST)
Protein
Triosephosphate isomerase
Gene
TPI1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 207000 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:5.3.1.1
: gluconeogenesis Pathwayi
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
Pathwayi: glycolysis
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate. This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 10 | Substrate1 Publication | 1 | |
Binding sitei | 12 | Substrate1 Publication | 1 | |
Active sitei | 95 | Electrophile | 1 | |
Active sitei | 165 | Proton acceptor | 1 |
GO - Molecular functioni
- triose-phosphate isomerase activity Source: SGD
GO - Biological processi
- gluconeogenesis Source: GO_Central
- glyceraldehyde-3-phosphate biosynthetic process Source: GO_Central
- glycerol catabolic process Source: GO_Central
- glycolytic process Source: SGD
Keywordsi
Molecular function | Isomerase |
Biological process | Gluconeogenesis, Glycolysis |
Enzyme and pathway databases
BRENDAi | 5.3.1.1, 984 |
Reactomei | R-SCE-70171, Glycolysis R-SCE-70263, Gluconeogenesis |
SABIO-RKi | P00942 |
UniPathwayi | UPA00109;UER00189 UPA00138 |
Names & Taxonomyi
Protein namesi | Recommended name: Triosephosphate isomerase (EC:5.3.1.1)Short name: TIM Alternative name(s): Triose-phosphate isomerase |
Gene namesi | Name:TPI1 Ordered Locus Names:YDR050C ORF Names:YD9609.05C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002457, TPI1 |
VEuPathDBi | FungiDB:YDR050C |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Mitochondrion
- mitochondrion Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000090169 | 2 – 248 | Triosephosphate isomeraseAdd BLAST | 247 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 4 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 71 | PhosphoserineCombined sources | 1 | |
Modified residuei | 215 | PhosphoserineCombined sources | 1 | |
Cross-linki | 223 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P00942 |
PaxDbi | P00942 |
PRIDEi | P00942 |
TopDownProteomicsi | P00942 |
2D gel databases
COMPLUYEAST-2DPAGEi | P00942 |
SWISS-2DPAGEi | P00942 |
PTM databases
iPTMneti | P00942 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 32104, 158 interactors |
DIPi | DIP-6671N |
IntActi | P00942, 14 interactors |
MINTi | P00942 |
STRINGi | 4932.YDR050C |
Miscellaneous databases
RNActi | P00942, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P00942 |
PCDDBi | P00942 |
SMRi | P00942 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00942 |
Family & Domainsi
Sequence similaritiesi
Belongs to the triosephosphate isomerase family.Curated
Phylogenomic databases
eggNOGi | KOG1643, Eukaryota |
GeneTreei | ENSGT00390000013354 |
HOGENOMi | CLU_024251_2_0_1 |
InParanoidi | P00942 |
OMAi | QEVCGAI |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00942-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARTFFVGGN FKLNGSKQSI KEIVERLNTA SIPENVEVVI CPPATYLDYS
60 70 80 90 100
VSLVKKPQVT VGAQNAYLKA SGAFTGENSV DQIKDVGAKW VILGHSERRS
110 120 130 140 150
YFHEDDKFIA DKTKFALGQG VGVILCIGET LEEKKAGKTL DVVERQLNAV
160 170 180 190 200
LEEVKDWTNV VVAYEPVWAI GTGLAATPED AQDIHASIRK FLASKLGDKA
210 220 230 240
ASELRILYGG SANGSNAVTF KDKADVDGFL VGGASLKPEF VDIINSRN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01366 Genomic DNA Translation: AAA88757.1 Z49209 Genomic DNA Translation: CAA89080.1 AY557654 Genomic DNA Translation: AAS55980.1 BK006938 Genomic DNA Translation: DAA11897.1 |
PIRi | A01168, ISBYT |
RefSeqi | NP_010335.1, NM_001180358.1 |
Genome annotation databases
EnsemblFungii | YDR050C_mRNA; YDR050C; YDR050C |
GeneIDi | 851620 |
KEGGi | sce:YDR050C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01366 Genomic DNA Translation: AAA88757.1 Z49209 Genomic DNA Translation: CAA89080.1 AY557654 Genomic DNA Translation: AAS55980.1 BK006938 Genomic DNA Translation: DAA11897.1 |
PIRi | A01168, ISBYT |
RefSeqi | NP_010335.1, NM_001180358.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1I45 | X-ray | 1.80 | A/B | 2-248 | [»] | |
1NEY | X-ray | 1.20 | A/B | 2-248 | [»] | |
1NF0 | X-ray | 1.60 | A/B | 2-248 | [»] | |
1YPI | X-ray | 1.90 | A/B | 2-248 | [»] | |
2YPI | X-ray | 2.50 | A/B | 2-248 | [»] | |
3YPI | X-ray | 2.80 | A/B | 2-248 | [»] | |
4FF7 | X-ray | 1.86 | A/B | 1-248 | [»] | |
7TIM | X-ray | 1.90 | A/B | 2-248 | [»] | |
BMRBi | P00942 | |||||
PCDDBi | P00942 | |||||
SMRi | P00942 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32104, 158 interactors |
DIPi | DIP-6671N |
IntActi | P00942, 14 interactors |
MINTi | P00942 |
STRINGi | 4932.YDR050C |
PTM databases
iPTMneti | P00942 |
2D gel databases
COMPLUYEAST-2DPAGEi | P00942 |
SWISS-2DPAGEi | P00942 |
Proteomic databases
MaxQBi | P00942 |
PaxDbi | P00942 |
PRIDEi | P00942 |
TopDownProteomicsi | P00942 |
Genome annotation databases
EnsemblFungii | YDR050C_mRNA; YDR050C; YDR050C |
GeneIDi | 851620 |
KEGGi | sce:YDR050C |
Organism-specific databases
SGDi | S000002457, TPI1 |
VEuPathDBi | FungiDB:YDR050C |
Phylogenomic databases
eggNOGi | KOG1643, Eukaryota |
GeneTreei | ENSGT00390000013354 |
HOGENOMi | CLU_024251_2_0_1 |
InParanoidi | P00942 |
OMAi | QEVCGAI |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00189 UPA00138 |
BRENDAi | 5.3.1.1, 984 |
Reactomei | R-SCE-70171, Glycolysis R-SCE-70263, Gluconeogenesis |
SABIO-RKi | P00942 |
Miscellaneous databases
EvolutionaryTracei | P00942 |
PROi | PR:P00942 |
RNActi | P00942, protein |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TPIS_YEAST | |
Accessioni | P00942Primary (citable) accession number: P00942 Secondary accession number(s): D6VS37 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 217 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families