UniProtKB - P00942 (TPIS_YEAST)

BLAST

>sp|P00942|TPIS_YEAST Triosephosphate isomerase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPI1 PE=1 SV=2
MARTFFVGGNFKLNGSKQSIKEIVERLNTASIPENVEVVICPPATYLDYSVSLVKKPQVT
VGAQNAYLKASGAFTGENSVDQIKDVGAKWVILGHSERRSYFHEDDKFIADKTKFALGQG
VGVILCIGETLEEKKAGKTLDVVERQLNAVLEEVKDWTNVVVAYEPVWAIGTGLAATPED
AQDIHASIRKFLASKLGDKAASELRILYGGSANGSNAVTFKDKADVDGFLVGGASLKPEF
VDIINSRN

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History
Entry version 197 (07 Nov 2018)
Sequence version 2 (23 Jan 2007)
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Protein

Triosephosphate isomerase

Gene

TPI1

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Miscellaneous

Present with 207000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayⁱ: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayⁱ: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:

Triosephosphate isomerase (TPI1)

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	10	Substrate1 Publication	1
Binding siteⁱ	12	Substrate1 Publication	1
Active siteⁱ	95	Electrophile	1
Active siteⁱ	165	Proton acceptor	1

GO - Molecular functionⁱ

triose-phosphate isomerase activity
Source: SGD
Inferred from direct assayⁱ
- 236471

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

gluconeogenesis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
glyceraldehyde-3-phosphate biosynthetic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
glycerol catabolic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
glycolytic process
Source: SGD
Inferred from mutant phenotypeⁱ
- 11329176

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Isomerase
Biological process	Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCycⁱ	YEAST:YDR050C-MONOMER
BRENDAⁱ	5.3.1.1 984
Reactomeⁱ	R-SCE-70171 Glycolysis R-SCE-70263 Gluconeogenesis
SABIO-RKⁱ	P00942
UniPathwayⁱ	UPA00109;UER00189 UPA00138

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Triosephosphate isomerase (EC:5.3.1.1) Short name: TIM Alternative name(s): Triose-phosphate isomerase
Gene namesⁱ	Name:TPI1 Ordered Locus Names:YDR050C ORF Names:YD9609.05C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome IV

Organism-specific databases

Saccharomyces Genome Database More...SGDⁱ	S000002457 TPI1

SGDⁱ

S000002457 TPI1

Subcellular locationⁱ

GO - Cellular component

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed1 Publication
ChainⁱPRO_0000090169	2 – 248	Triosephosphate isomeraseAdd BLAST		247

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	4	PhosphothreonineCombined sources	1
Modified residueⁱ	71	PhosphoserineCombined sources	1
Modified residueⁱ	215	PhosphoserineCombined sources	1
Cross-linkⁱ	223	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P00942
PaxDbⁱ	P00942
PRIDEⁱ	P00942
TopDownProteomicsⁱ	P00942

2D gel databases

COMPLUYEAST-2DPAGEⁱ	P00942
SWISS-2DPAGEⁱ	P00942

PTM databases

iPTMnetⁱ	P00942

iPTMnetⁱ

P00942

Interactionⁱ

Subunit structureⁱ

Homodimer.1 Publication

Protein-protein interaction databases

BioGridⁱ	32104, 128 interactors
Database of interacting proteins More...DIPⁱ	DIP-6671N
IntActⁱ	P00942, 10 interactors
Molecular INTeraction database More...MINTⁱ	P00942
STRINGⁱ	4932.YDR050C

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	5 – 10	Combined sources	6
Helixⁱ	17 – 29	Combined sources	13
Beta strandⁱ	36 – 41	Combined sources	6
Helixⁱ	44 – 46	Combined sources	3
Helixⁱ	47 – 53	Combined sources	7
Beta strandⁱ	59 – 64	Combined sources	6
Beta strandⁱ	68 – 73	Combined sources	6
Helixⁱ	80 – 85	Combined sources	6
Beta strandⁱ	90 – 94	Combined sources	5
Helixⁱ	96 – 100	Combined sources	5
Helixⁱ	106 – 118	Combined sources	13
Beta strandⁱ	122 – 127	Combined sources	6
Helixⁱ	131 – 135	Combined sources	5
Helixⁱ	139 – 153	Combined sources	15
Beta strandⁱ	160 – 164	Combined sources	5
Helixⁱ	167 – 169	Combined sources	3
Turnⁱ	170 – 172	Combined sources	3
Helixⁱ	178 – 196	Combined sources	19
Helixⁱ	198 – 203	Combined sources	6
Beta strandⁱ	206 – 211	Combined sources	6
Turnⁱ	214 – 216	Combined sources	3
Helixⁱ	217 – 220	Combined sources	4
Beta strandⁱ	228 – 232	Combined sources	5
Helixⁱ	233 – 236	Combined sources	4
Helixⁱ	239 – 244	Combined sources	6
Turnⁱ	245 – 247	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P00942
SMRⁱ	P00942
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P00942

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000013354
HOGENOMⁱ	HOG000226413
InParanoidⁱ	P00942
KEGG Orthology (KO) More...KOⁱ	K01803
OMAⁱ	LCVGEGL
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C484E

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00311 TIM, 1 hit
Gene3Dⁱ	3.20.20.70, 1 hit
HAMAPⁱ	MF_00147_B TIM_B, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR035990 TIM_sf IPR022896 TrioseP_Isoase_bac/euk IPR000652 Triosephosphate_isomerase IPR020861 Triosephosphate_isomerase_AS
PANTHERⁱ	PTHR21139 PTHR21139, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00121 TIM, 1 hit
SUPFAMⁱ	SSF51351 SSF51351, 1 hit
TIGRFAMsⁱ	TIGR00419 tim, 1 hit
PROSITEⁱ	View protein in PROSITE PS00171 TIM_1, 1 hit PS51440 TIM_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P00942-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MARTFFVGGN FKLNGSKQSI KEIVERLNTA SIPENVEVVI CPPATYLDYS 
        60         70         80         90        100
VSLVKKPQVT VGAQNAYLKA SGAFTGENSV DQIKDVGAKW VILGHSERRS 
       110        120        130        140        150
YFHEDDKFIA DKTKFALGQG VGVILCIGET LEEKKAGKTL DVVERQLNAV 
       160        170        180        190        200
LEEVKDWTNV VVAYEPVWAI GTGLAATPED AQDIHASIRK FLASKLGDKA 
       210        220        230        240 
ASELRILYGG SANGSNAVTF KDKADVDGFL VGGASLKPEF VDIINSRN

Length:248

Mass (Da):26,795

Last modified:January 23, 2007 - v2

Checksum:ⁱ8429251C4DF51F86

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J01366 Genomic DNA Translation: AAA88757.1 Z49209 Genomic DNA Translation: CAA89080.1 AY557654 Genomic DNA Translation: AAS55980.1 BK006938 Genomic DNA Translation: DAA11897.1
PIRⁱ	A01168 ISBYT
NCBI Reference Sequences More...RefSeqⁱ	NP_010335.1, NM_001180358.1

Genome annotation databases

EnsemblFungiⁱ	YDR050C; YDR050C; YDR050C
GeneIDⁱ	851620
KEGGⁱ	sce:YDR050C

Protein	Similar proteins		Species	Score	Length	Source
P00942	Triosephosphate isomerase		YEASB		248	UniRef100_P00942
Triosephosphate isomerase		YEAS1		248
Triosephosphate isomerase		YEASK		248
Triosephosphate isomerase		YEASA		248
Triosephosphate isomerase		YEAS8		248
+9

Protein	Similar proteins		Species	Score	Length	Source
P00942	Triosephosphate isomerase		YEASB		248	UniRef90_P00942
Triosephosphate isomerase		YEAS1		248
Triosephosphate isomerase		YEASK		248
Triosephosphate isomerase		YEASA		248
Triosephosphate isomerase		YEAS8		248
+19

Protein	Similar proteins		Species	Score	Length	Source
P00942	Triosephosphate isomerase		YEASB		248	UniRef50_P00942
Triosephosphate isomerase		YEAS1		248
Triosephosphate isomerase		YEASK		248
Triosephosphate isomerase		YEASA		248
Triosephosphate isomerase		YEAS8		248
+94

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J01366 Genomic DNA Translation: AAA88757.1 Z49209 Genomic DNA Translation: CAA89080.1 AY557654 Genomic DNA Translation: AAS55980.1 BK006938 Genomic DNA Translation: DAA11897.1
PIRⁱ	A01168 ISBYT
RefSeqⁱ	NP_010335.1, NM_001180358.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1I45	X-ray	1.80	A/B	2-248	[»]
	1NEY	X-ray	1.20	A/B	2-248	[»]
	1NF0	X-ray	1.60	A/B	2-248	[»]
	1YPI	X-ray	1.90	A/B	2-248	[»]
	2YPI	X-ray	2.50	A/B	2-248	[»]
	3YPI	X-ray	2.80	A/B	2-248	[»]
	4FF7	X-ray	1.86	A/B	1-248	[»]
	7TIM	X-ray	1.90	A/B	2-248	[»]
ProteinModelPortalⁱ	P00942
SMRⁱ	P00942
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32104, 128 interactors
DIPⁱ	DIP-6671N
IntActⁱ	P00942, 10 interactors
MINTⁱ	P00942
STRINGⁱ	4932.YDR050C

PTM databases

iPTMnetⁱ	P00942

iPTMnetⁱ

P00942

2D gel databases

COMPLUYEAST-2DPAGEⁱ	P00942
SWISS-2DPAGEⁱ	P00942

Proteomic databases

MaxQBⁱ	P00942
PaxDbⁱ	P00942
PRIDEⁱ	P00942
TopDownProteomicsⁱ	P00942

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YDR050C; YDR050C; YDR050C
GeneIDⁱ	851620
KEGGⁱ	sce:YDR050C

Organism-specific databases

SGDⁱ	S000002457 TPI1

Phylogenomic databases

GeneTreeⁱ	ENSGT00390000013354
HOGENOMⁱ	HOG000226413
InParanoidⁱ	P00942
KOⁱ	K01803
OMAⁱ	LCVGEGL
OrthoDBⁱ	EOG092C484E

Enzyme and pathway databases

UniPathwayⁱ	UPA00109;UER00189 UPA00138
BioCycⁱ	YEAST:YDR050C-MONOMER
BRENDAⁱ	5.3.1.1 984
Reactomeⁱ	R-SCE-70171 Glycolysis R-SCE-70263 Gluconeogenesis
SABIO-RKⁱ	P00942

Miscellaneous databases

EvolutionaryTraceⁱ	P00942
Protein Ontology More...PROⁱ	PR:P00942

Family and domain databases

CDDⁱ	cd00311 TIM, 1 hit
Gene3Dⁱ	3.20.20.70, 1 hit
HAMAPⁱ	MF_00147_B TIM_B, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR035990 TIM_sf IPR022896 TrioseP_Isoase_bac/euk IPR000652 Triosephosphate_isomerase IPR020861 Triosephosphate_isomerase_AS
PANTHERⁱ	PTHR21139 PTHR21139, 1 hit
Pfamⁱ	View protein in Pfam PF00121 TIM, 1 hit
SUPFAMⁱ	SSF51351 SSF51351, 1 hit
TIGRFAMsⁱ	TIGR00419 tim, 1 hit
PROSITEⁱ	View protein in PROSITE PS00171 TIM_1, 1 hit PS51440 TIM_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TPIS_YEAST
Accessionⁱ	P00942Primary (citable) accession number: P00942 Secondary accession number(s): D6VS37
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 197 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

UniProtKB

UniParc

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UniRef

Proteomes

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Supporting data

UniProtKB - P00942 (TPIS_YEAST)

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Triosephosphate isomerase

TPI1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: gluconeogenesis

Pathwayi: glycolysis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytosol

Mitochondrion

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: gluconeogenesis

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ