TPI1

Functionⁱ

Catalytic activityⁱ

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayⁱ: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayⁱ: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:

Triosephosphate isomerase (TPI1), Triosephosphate isomerase, Triosephosphate isomerase (TPI1)

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	13	Substrate	1
Active siteⁱ	95	Electrophile	1
Active siteⁱ	165	Proton acceptor	1

GO - Molecular functionⁱ

triose-phosphate isomerase activity Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

gluconeogenesis Source: GO_Central
glyceraldehyde-3-phosphate biosynthetic process Source: GO_Central
glycerol catabolic process Source: GO_Central
glycolytic process Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Isomerase
Biological process	Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BRENDAⁱ	5.3.1.1 1306
Reactomeⁱ	R-GGA-352875 Gluconeogenesis R-GGA-352882 Glycolysis R-GGA-70171 Glycolysis R-GGA-70263 Gluconeogenesis
SABIO-RKⁱ	P00940
UniPathwayⁱ	UPA00109;UER00189 UPA00138

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Triosephosphate isomerase (EC:5.3.1.1) Short name: TIM Alternative name(s): Triose-phosphate isomerase
Gene namesⁱ	Name:TPI1
Organismⁱ	Gallus gallus (Chicken)
Taxonomic identifierⁱ	9031 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Sauropsida › Sauria › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus
Proteomesⁱ	UP000000539 Componentⁱ: Chromosome 1

Subcellular locationⁱ

GO - Cellular component

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	95	H → N: Reduces activity 5000-fold. 1 Publication		1
Mutagenesisⁱ	165	E → D: Reduces activity 300-fold. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed1 Publication
ChainⁱPRO_0000090121	2 – 248	Triosephosphate isomeraseAdd BLAST		247

Proteomic databases

PaxDbⁱ	P00940
PRIDEⁱ	P00940

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSGALG00000014526 Expressed in 11 organ(s), highest expression level in skeletal muscle tissue

Bgeeⁱ

ENSGALG00000014526 Expressed in 11 organ(s), highest expression level in skeletal muscle tissue

Interactionⁱ

Subunit structureⁱ

Homodimer.1 Publication

Protein-protein interaction databases

BioGridⁱ	676684, 1 interactor
IntActⁱ	P00940, 1 interactor
STRINGⁱ	9031.ENSGALP00000023396

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	6 – 11	Combined sources	6
Helixⁱ	18 – 30	Combined sources	13
Beta strandⁱ	37 – 43	Combined sources	7
Helixⁱ	45 – 47	Combined sources	3
Helixⁱ	48 – 54	Combined sources	7
Beta strandⁱ	59 – 64	Combined sources	6
Beta strandⁱ	68 – 73	Combined sources	6
Helixⁱ	80 – 85	Combined sources	6
Beta strandⁱ	90 – 94	Combined sources	5
Helixⁱ	96 – 100	Combined sources	5
Helixⁱ	106 – 118	Combined sources	13
Beta strandⁱ	122 – 127	Combined sources	6
Helixⁱ	131 – 136	Combined sources	6
Helixⁱ	139 – 151	Combined sources	13
Helixⁱ	157 – 159	Combined sources	3
Beta strandⁱ	160 – 164	Combined sources	5
Helixⁱ	167 – 169	Combined sources	3
Beta strandⁱ	170 – 173	Combined sources	4
Helixⁱ	178 – 195	Combined sources	18
Helixⁱ	198 – 203	Combined sources	6
Beta strandⁱ	206 – 208	Combined sources	3
Turnⁱ	214 – 216	Combined sources	3
Helixⁱ	217 – 221	Combined sources	5
Beta strandⁱ	228 – 232	Combined sources	5
Helixⁱ	233 – 236	Combined sources	4
Helixⁱ	240 – 244	Combined sources	5
Turnⁱ	245 – 247	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P00940
SMRⁱ	P00940
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P00940

EvolutionaryTraceⁱ

P00940

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1643 Eukaryota COG0149 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000013354
HOGENOMⁱ	HOG000226413
HOVERGENⁱ	HBG002599
InParanoidⁱ	P00940
KEGG Orthology (KO) More...KOⁱ	K01803
OMAⁱ	LCVGEGL
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0LEZ
PhylomeDBⁱ	P00940
TreeFamⁱ	TF300829

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00311 TIM, 1 hit
Gene3Dⁱ	3.20.20.70, 1 hit
HAMAPⁱ	MF_00147_B TIM_B, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR035990 TIM_sf IPR022896 TrioseP_Isoase_bac/euk IPR000652 Triosephosphate_isomerase IPR020861 Triosephosphate_isomerase_AS
PANTHERⁱ	PTHR21139 PTHR21139, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00121 TIM, 1 hit
SUPFAMⁱ	SSF51351 SSF51351, 1 hit
TIGRFAMsⁱ	TIGR00419 tim, 1 hit
PROSITEⁱ	View protein in PROSITE PS00171 TIM_1, 1 hit PS51440 TIM_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.ⁱShow all

P00940-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAPRKFFVGG NWKMNGDKKS LGELIHTLNG AKLSADTEVV CGAPSIYLDF 
        60         70         80         90        100
ARQKLDAKIG VAAQNCYKVP KGAFTGEISP AMIKDIGAAW VILGHSERRH 
       110        120        130        140        150
VFGESDELIG QKVAHALAEG LGVIACIGEK LDEREAGITE KVVFEQTKAI 
       160        170        180        190        200
ADNVKDWSKV VLAYEPVWAI GTGKTATPQQ AQEVHEKLRG WLKSHVSDAV 
       210        220        230        240 
AQSTRIIYGG SVTGGNCKEL ASQHDVDGFL VGGASLKPEF VDIINAKH

Length:248

Mass (Da):26,620

Last modified:January 23, 2007 - v2

Checksum:ⁱAFCC258E574DE982

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	A0A1D5PI46	A0A1D5PI46_CHICK		Triosephosphate isomerase Triosephosphate isomerase, EC 5.3.1.1	TPI1	190	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	17 – 18	DK → KR AA sequence (PubMed:4463937).Curated	2
Sequence conflictⁱ	29	N → D AA sequence (PubMed:4463937).Curated	1
Sequence conflictⁱ	145 – 146	EQ → QE AA sequence (PubMed:4463937).Curated	2
Sequence conflictⁱ	194	S → T AA sequence (PubMed:4463937).Curated	1
Sequence conflictⁱ	202 – 204	QST → VQS AA sequence (PubMed:4463937).Curated	3

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M11314 mRNA Translation: AAA49094.1 M11941 Genomic DNA Translation: AAA49095.1
PIRⁱ	A23448 ISCHT
NCBI Reference Sequences More...RefSeqⁱ	NP_990782.1, NM_205451.1
UniGeneⁱ	Gga.4148

Genome annotation databases

Ensemblⁱ	ENSGALT00000023442; ENSGALP00000023396; ENSGALG00000014526 ENSGALT00000071768; ENSGALP00000044536; ENSGALG00000014526
GeneIDⁱ	396435
KEGGⁱ	gga:396435

Protein	Similar proteins		Species	Score	Length	Source
P00940	Triosephosphate isomerase (Fragment)		MELGA		85	UniRef100_P00940
Triosephosphate isomerase (Fragment)		EURHL		211
UPI0005280388		EURHL		167

Protein	Similar proteins		Species	Score	Length	Source
P00940	Triosephosphate isomerase		CANLF		249	UniRef90_P00940
Triosephosphate isomerase		BOVIN		249
Triosephosphate isomerase	)	RABIT		248
Triosephosphate isomerase (Fragment)		MELGA		85
Triosephosphate isomerase (Fragment)		EURHL		211
+78

Protein	Similar proteins		Species	Score	Length	Source
P00940	Triosephosphate isomerase	)	HUMAN		286	UniRef50_P60174
Triosephosphate isomerase		PANTR		249
Triosephosphate isomerase		MOUSE		299
Triosephosphate isomerase		RAT		249
Triosephosphate isomerase		MACFA		249
+255

Entry informationⁱ

Entry nameⁱ	TPIS_CHICK
Accessionⁱ	P00940Primary (citable) accession number: P00940
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	January 23, 2007
	Last modified:	September 12, 2018
	This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P00940 (TPIS_CHICK)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Triosephosphate isomerase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: gluconeogenesis

Pathwayi: glycolysis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytosol

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi