We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P00940 (TPIS_CHICK)
Protein
Triosephosphate isomerase
Gene
TPI1
Organism
Gallus gallus (Chicken)
Status
Functioni
Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.
By similarityIt is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.
By similarityCatalytic activityi
: glycolysis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.PROSITE-ProRule annotation This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.
Pathwayi: gluconeogenesis
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 11 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 13 | SubstrateCombined sources1 Publication | 1 | |
Active sitei | 95 | ElectrophileCombined sources1 Publication | 1 | |
Active sitei | 165 | Proton acceptorCombined sources1 Publication | 1 |
GO - Molecular functioni
- methylglyoxal synthase activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- triose-phosphate isomerase activity Source: UniProtKB
- ubiquitin protein ligase binding Source: Ensembl
GO - Biological processi
- gluconeogenesis Source: GO_Central
- glyceraldehyde-3-phosphate biosynthetic process Source: UniProtKB
- glycerol catabolic process Source: GO_Central
- glycolytic process Source: GO_Central
- methylglyoxal biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Isomerase, Lyase |
Biological process | Gluconeogenesis, Glycolysis |
Enzyme and pathway databases
BRENDAi | 5.3.1.1, 1306 |
Reactomei | R-GGA-352875, Gluconeogenesis R-GGA-352882, Glycolysis R-GGA-70171, Glycolysis R-GGA-70263, Gluconeogenesis |
SABIO-RKi | P00940 |
UniPathwayi | UPA00109;UER00189 UPA00138 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TPI1 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm PROSITE-ProRule annotation
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 95 | H → N: Reduces activity 5000-fold. 1 Publication | 1 | |
Mutagenesisi | 165 | E → D: Reduces activity 300-fold. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000090121 | 2 – 248 | Triosephosphate isomeraseAdd BLAST | 247 |
Proteomic databases
PaxDbi | P00940 |
Expressioni
Gene expression databases
Bgeei | ENSGALG00000014526, Expressed in skeletal muscle tissue and 14 other tissues |
Interactioni
Subunit structurei
Homodimer.
PROSITE-ProRule annotation1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- ubiquitin protein ligase binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 676684, 1 interactor |
IntActi | P00940, 1 interactor |
STRINGi | 9031.ENSGALP00000023396 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P00940 |
SMRi | P00940 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00940 |
Family & Domainsi
Sequence similaritiesi
Belongs to the triosephosphate isomerase family.Curated
Phylogenomic databases
eggNOGi | KOG1643, Eukaryota |
GeneTreei | ENSGT00390000013354 |
HOGENOMi | CLU_024251_2_0_1 |
InParanoidi | P00940 |
OMAi | QEVCGAI |
OrthoDBi | 1272577at2759 |
PhylomeDBi | P00940 |
TreeFami | TF300829 |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00940-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPRKFFVGG NWKMNGDKKS LGELIHTLNG AKLSADTEVV CGAPSIYLDF
60 70 80 90 100
ARQKLDAKIG VAAQNCYKVP KGAFTGEISP AMIKDIGAAW VILGHSERRH
110 120 130 140 150
VFGESDELIG QKVAHALAEG LGVIACIGEK LDEREAGITE KVVFEQTKAI
160 170 180 190 200
ADNVKDWSKV VLAYEPVWAI GTGKTATPQQ AQEVHEKLRG WLKSHVSDAV
210 220 230 240
AQSTRIIYGG SVTGGNCKEL ASQHDVDGFL VGGASLKPEF VDIINAKH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 17 – 18 | DK → KR AA sequence (PubMed:4463937).Curated | 2 | |
Sequence conflicti | 29 | N → D AA sequence (PubMed:4463937).Curated | 1 | |
Sequence conflicti | 145 – 146 | EQ → QE AA sequence (PubMed:4463937).Curated | 2 | |
Sequence conflicti | 194 | S → T AA sequence (PubMed:4463937).Curated | 1 | |
Sequence conflicti | 202 – 204 | QST → VQS AA sequence (PubMed:4463937).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11314 mRNA Translation: AAA49094.1 M11941 Genomic DNA Translation: AAA49095.1 |
PIRi | A23448, ISCHT |
RefSeqi | NP_990782.1, NM_205451.1 |
Genome annotation databases
Ensembli | ENSGALT00000023442; ENSGALP00000023396; ENSGALG00000014526 ENSGALT00000071768; ENSGALP00000044536; ENSGALG00000014526 |
GeneIDi | 396435 |
KEGGi | gga:396435 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11314 mRNA Translation: AAA49094.1 M11941 Genomic DNA Translation: AAA49095.1 |
PIRi | A23448, ISCHT |
RefSeqi | NP_990782.1, NM_205451.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SPQ | X-ray | 2.16 | A/B | 2-248 | [»] | |
1SQ7 | X-ray | 2.85 | A/B | 2-248 | [»] | |
1SSD | X-ray | 2.90 | A/B | 2-248 | [»] | |
1SSG | X-ray | 2.90 | A/B | 2-248 | [»] | |
1SU5 | X-ray | 2.70 | A/B | 2-248 | [»] | |
1SW0 | X-ray | 1.71 | A/B | 1-248 | [»] | |
1SW3 | X-ray | 2.03 | A/B | 1-248 | [»] | |
1SW7 | X-ray | 2.22 | A/B | 1-248 | [»] | |
1TIM | X-ray | 2.50 | A/B | 2-248 | [»] | |
1TPB | X-ray | 1.90 | 1/2 | 2-248 | [»] | |
1TPC | X-ray | 1.90 | 1/2 | 2-248 | [»] | |
1TPH | X-ray | 1.80 | 1/2 | 2-248 | [»] | |
1TPU | X-ray | 1.90 | A/B | 2-248 | [»] | |
1TPV | X-ray | 1.90 | A/B | 2-248 | [»] | |
1TPW | X-ray | 1.90 | A/B | 2-248 | [»] | |
4P61 | X-ray | 1.34 | A/B | 1-248 | [»] | |
8TIM | X-ray | 2.50 | A/B | 2-248 | [»] | |
BMRBi | P00940 | |||||
SMRi | P00940 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 676684, 1 interactor |
IntActi | P00940, 1 interactor |
STRINGi | 9031.ENSGALP00000023396 |
Proteomic databases
PaxDbi | P00940 |
Genome annotation databases
Ensembli | ENSGALT00000023442; ENSGALP00000023396; ENSGALG00000014526 ENSGALT00000071768; ENSGALP00000044536; ENSGALG00000014526 |
GeneIDi | 396435 |
KEGGi | gga:396435 |
Organism-specific databases
CTDi | 7167 |
Phylogenomic databases
eggNOGi | KOG1643, Eukaryota |
GeneTreei | ENSGT00390000013354 |
HOGENOMi | CLU_024251_2_0_1 |
InParanoidi | P00940 |
OMAi | QEVCGAI |
OrthoDBi | 1272577at2759 |
PhylomeDBi | P00940 |
TreeFami | TF300829 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00189 UPA00138 |
BRENDAi | 5.3.1.1, 1306 |
Reactomei | R-GGA-352875, Gluconeogenesis R-GGA-352882, Glycolysis R-GGA-70171, Glycolysis R-GGA-70263, Gluconeogenesis |
SABIO-RKi | P00940 |
Miscellaneous databases
EvolutionaryTracei | P00940 |
PROi | PR:P00940 |
Gene expression databases
Bgeei | ENSGALG00000014526, Expressed in skeletal muscle tissue and 14 other tissues |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TPIS_CHICK | |
Accessioni | P00940Primary (citable) accession number: P00940 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | September 29, 2021 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families