UniProtKB - P00935 (METB_ECOLI)
Protein
Cystathionine gamma-synthase
Gene
metB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia.1 Publication
Catalytic activityi
- EC:2.5.1.481 Publication
Cofactori
pyridoxal 5'-phosphate1 PublicationNote: Binds 1 pyridoxal phosphate per subunit.1 Publication
Kineticsi
kcat are 700 min(-1) and 460 min (-1) for the gamma-replacement and gamma-elimination reaction, respectively.
- KM=0.33 mM for O-succinyl-L-homoserine (at pH 8.2 and 25 degrees Celsius, when assaying the gamma-elimination reaction)1 Publication
- KM=1.0 mM for O-succinyl-L-homoserine (at pH 8.2 and 25 degrees Celsius, when assaying the gamma-replacement reaction)1 Publication
- KM=0.05 mM for L-cysteine (at pH 8.2 and 25 degrees Celsius, when assaying the gamma-replacement reaction)1 Publication
pH dependencei
Optimum pH is 7.8 for the gamma-replacement reaction.1 Publication
: L-methionine biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.Proteins known to be involved in this subpathway in this organism are:
- Cystathionine gamma-synthase (metB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.
GO - Molecular functioni
- carbon-sulfur lyase activity Source: GO_Central
- cystathionine gamma-lyase activity Source: GO_Central
- cystathionine gamma-synthase activity Source: EcoCyc
- cystathionine gamma-synthase activity (acts on O-phosphohomoserine) Source: UniProtKB-EC
- pyridoxal phosphate binding Source: EcoCyc
GO - Biological processi
- cysteine biosynthetic process via cystathionine Source: GO_Central
- methionine biosynthetic process Source: EcoCyc
- transsulfuration Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Amino-acid biosynthesis, Methionine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:O-SUCCHOMOSERLYASE-MONOMER MetaCyc:O-SUCCHOMOSERLYASE-MONOMER |
BRENDAi | 2.5.1.48, 2026 |
SABIO-RKi | P00935 |
UniPathwayi | UPA00051;UER00077 |
Names & Taxonomyi
Protein namesi | Recommended name: Cystathionine gamma-synthase (EC:2.5.1.48)Short name: CGS Alternative name(s): O-succinylhomoserine (thiol)-lyase |
Gene namesi | Name:metB Ordered Locus Names:b3939, JW3910 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000114756 | 1 – 386 | Cystathionine gamma-synthaseAdd BLAST | 386 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 198 | N6-(pyridoxal phosphate)lysine1 Publication | 1 |
Proteomic databases
jPOSTi | P00935 |
PaxDbi | P00935 |
PRIDEi | P00935 |
Interactioni
Subunit structurei
Homotetramer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261102, 19 interactors |
DIPi | DIP-10192N |
IntActi | P00935, 1 interactor |
STRINGi | 511145.b3939 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00935 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00935 |
Family & Domainsi
Sequence similaritiesi
Belongs to the trans-sulfuration enzymes family.Curated
Phylogenomic databases
eggNOGi | COG0626, Bacteria |
HOGENOMi | CLU_018986_2_0_6 |
InParanoidi | P00935 |
PhylomeDBi | P00935 |
Family and domain databases
CDDi | cd00614, CGS_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000277, Cys/Met-Metab_PyrdxlP-dep_enz IPR011821, O_succ_thio_ly IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11808, PTHR11808, 1 hit |
Pfami | View protein in Pfam PF01053, Cys_Met_Meta_PP, 1 hit |
PIRSFi | PIRSF001434, CGS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR02080, O_succ_thio_ly, 1 hit |
PROSITEi | View protein in PROSITE PS00868, CYS_MET_METAB_PP, 1 hit |
i Sequence
Sequence statusi: Complete.
P00935-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRKQATIAV RSGLNDDEQY GCVVPPIHLS STYNFTGFNE PRAHDYSRRG
60 70 80 90 100
NPTRDVVQRA LAELEGGAGA VLTNTGMSAI HLVTTVFLKP GDLLVAPHDC
110 120 130 140 150
YGGSYRLFDS LAKRGCYRVL FVDQGDEQAL RAALAEKPKL VLVESPSNPL
160 170 180 190 200
LRVVDIAKIC HLAREVGAVS VVDNTFLSPA LQNPLALGAD LVLHSCTKYL
210 220 230 240 250
NGHSDVVAGV VIAKDPDVVT ELAWWANNIG VTGGAFDSYL LLRGLRTLVP
260 270 280 290 300
RMELAQRNAQ AIVKYLQTQP LVKKLYHPSL PENQGHEIAA RQQKGFGAML
310 320 330 340 350
SFELDGDEQT LRRFLGGLSL FTLAESLGGV ESLISHAATM THAGMAPEAR
360 370 380
AAAGISETLL RISTGIEDGE DLIADLENGF RAANKG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01546 Genomic DNA Translation: AAA24167.1 L19201 Genomic DNA Translation: AAB03071.1 U00096 Genomic DNA Translation: AAC76921.1 AP009048 Genomic DNA Translation: BAE77371.1 |
PIRi | A01158, SYECCG |
RefSeqi | NP_418374.1, NC_000913.3 WP_001295694.1, NZ_SSZK01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC76921; AAC76921; b3939 BAE77371; BAE77371; BAE77371 |
GeneIDi | 58461007 948434 |
KEGGi | ecj:JW3910 eco:b3939 |
PATRICi | fig|1411691.4.peg.2766 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01546 Genomic DNA Translation: AAA24167.1 L19201 Genomic DNA Translation: AAB03071.1 U00096 Genomic DNA Translation: AAC76921.1 AP009048 Genomic DNA Translation: BAE77371.1 |
PIRi | A01158, SYECCG |
RefSeqi | NP_418374.1, NC_000913.3 WP_001295694.1, NZ_SSZK01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CS1 | X-ray | 1.50 | A/B/C/D | 1-386 | [»] | |
SMRi | P00935 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261102, 19 interactors |
DIPi | DIP-10192N |
IntActi | P00935, 1 interactor |
STRINGi | 511145.b3939 |
Chemistry databases
DrugBanki | DB04083, N(6)-(pyridoxal phosphate)-L-lysine |
Proteomic databases
jPOSTi | P00935 |
PaxDbi | P00935 |
PRIDEi | P00935 |
Genome annotation databases
EnsemblBacteriai | AAC76921; AAC76921; b3939 BAE77371; BAE77371; BAE77371 |
GeneIDi | 58461007 948434 |
KEGGi | ecj:JW3910 eco:b3939 |
PATRICi | fig|1411691.4.peg.2766 |
Organism-specific databases
EchoBASEi | EB0577 |
Phylogenomic databases
eggNOGi | COG0626, Bacteria |
HOGENOMi | CLU_018986_2_0_6 |
InParanoidi | P00935 |
PhylomeDBi | P00935 |
Enzyme and pathway databases
UniPathwayi | UPA00051;UER00077 |
BioCyci | EcoCyc:O-SUCCHOMOSERLYASE-MONOMER MetaCyc:O-SUCCHOMOSERLYASE-MONOMER |
BRENDAi | 2.5.1.48, 2026 |
SABIO-RKi | P00935 |
Miscellaneous databases
EvolutionaryTracei | P00935 |
PROi | PR:P00935 |
Family and domain databases
CDDi | cd00614, CGS_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000277, Cys/Met-Metab_PyrdxlP-dep_enz IPR011821, O_succ_thio_ly IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11808, PTHR11808, 1 hit |
Pfami | View protein in Pfam PF01053, Cys_Met_Meta_PP, 1 hit |
PIRSFi | PIRSF001434, CGS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR02080, O_succ_thio_ly, 1 hit |
PROSITEi | View protein in PROSITE PS00868, CYS_MET_METAB_PP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | METB_ECOLI | |
Accessioni | P00935Primary (citable) accession number: P00935 Secondary accession number(s): Q2M8N5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families