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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional protein TrpGD (trpGD), Anthranilate synthase component 1 (trpE)
  2. Bifunctional protein TrpGD (trpGD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei49Proton acceptor1
Active sitei60Proton acceptor1

GO - Molecular functioni

Keywordsi

Molecular functionAllosteric enzyme, Lyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-1744-MONOMER
UniPathwayi
UPA00035;UER00044

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotation
Ordered Locus Names:STM1727
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB07748 2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL DIHYDROGEN PHOSPHATE
DB07732 2-[(2-NAPHTHYLSULFONYL)AMINO]ETHYL DIHYDROGEN PHOSPHATE
DB07745 2-{[4-(TRIFLUOROMETHOXY)BENZOYL]AMINO}ETHYL DIHYDROGEN PHOSPHATE
DB07894 4-(2-HYDROXY-4-FLUOROPHENYLTHIO)-BUTYLPHOSPHONIC ACID
DB07925 4-(2-HYDROXYPHENYLSULFINYL)-BUTYLPHOSPHONIC ACID
DB07890 4-(2-HYDROXYPHENYLTHIO)-1-BUTENYLPHOSPHONIC ACID
DB07773 5-FLUOROINDOLE PROPANOL PHOSPHATE
DB04143 Indole-3-Glycerol Phosphate
DB03171 Indole-3-Propanol Phosphate
DB07951 N-[1H-INDOL-3-YL-ACETYL]ASPARTIC ACID
DB07952 N-[1H-INDOL-3-YL-ACETYL]GLYCINE ACID
DB07953 N-[1H-INDOL-3-YL-ACETYL]VALINE ACID

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000988391 – 268Tryptophan synthase alpha chainAdd BLAST268

Proteomic databases

PaxDbiP00929
PRIDEiP00929

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
trpBP0A2K124EBI-1028423,EBI-1028431

Protein-protein interaction databases

DIPiDIP-1033N
IntActiP00929, 1 interactor
MINTiP00929
STRINGi99287.STM1727

Structurei

Secondary structure

1268
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00929
SMRiP00929
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00929

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F6H Bacteria
COG0159 LUCA
HOGENOMiHOG000223814
KOiK01695
OMAiDYPPEEC
PhylomeDBiP00929

Family and domain databases

CDDicd04724 Tryptophan_synthase_alpha, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00131 Trp_synth_alpha, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011060 RibuloseP-bd_barrel
IPR018204 Trp_synthase_alpha_AS
IPR002028 Trp_synthase_suA
PfamiView protein in Pfam
PF00290 Trp_syntA, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR00262 trpA, 1 hit
PROSITEiView protein in PROSITE
PS00167 TRP_SYNTHASE_ALPHA, 1 hit

Sequencei

Sequence statusi: Complete.

P00929-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERYENLFAQ LNDRREGAFV PFVTLGDPGI EQSLKIIDTL IDAGADALEL
60 70 80 90 100
GVPFSDPLAD GPTIQNANLR AFAAGVTPAQ CFEMLALIRE KHPTIPIGLL
110 120 130 140 150
MYANLVFNNG IDAFYARCEQ VGVDSVLVAD VPVEESAPFR QAALRHNIAP
160 170 180 190 200
IFICPPNADD DLLRQVASYG RGYTYLLSRS GVTGAENRGA LPLHHLIEKL
210 220 230 240 250
KEYHAAPALQ GFGISSPEQV SAAVRAGAAG AISGSAIVKI IEKNLASPKQ
260
MLAELRSFVS AMKAASRA
Length:268
Mass (Da):28,671
Last modified:July 21, 1986 - v1
Checksum:iF409BF1A931581B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01376 Genomic DNA Translation: CAA24666.1
J01810 Genomic DNA Translation: AAA27235.1
AE006468 Genomic DNA Translation: AAL20645.1
PIRiA93837 TSEBAT
RefSeqiNP_460686.1, NC_003197.2
WP_000443030.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL20645; AAL20645; STM1727
GeneIDi1253246
KEGGistm:STM1727
PATRICifig|99287.12.peg.1823

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01376 Genomic DNA Translation: CAA24666.1
J01810 Genomic DNA Translation: AAA27235.1
AE006468 Genomic DNA Translation: AAL20645.1
PIRiA93837 TSEBAT
RefSeqiNP_460686.1, NC_003197.2
WP_000443030.1, NC_003197.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A50X-ray2.30A1-268[»]
1A5AX-ray1.90A1-268[»]
1A5BX-ray2.00A1-268[»]
1A5SX-ray2.30A1-268[»]
1BEUX-ray1.90A1-268[»]
1BKSX-ray2.20A1-268[»]
1C29X-ray2.30A1-268[»]
1C8VX-ray2.20A1-268[»]
1C9DX-ray2.30A1-268[»]
1CW2X-ray2.00A1-268[»]
1CX9X-ray2.30A1-268[»]
1FUYX-ray2.25A1-268[»]
1K3UX-ray1.70A1-268[»]
1K7EX-ray2.30A1-268[»]
1K7FX-ray1.90A1-268[»]
1K7XX-ray1.70A1-268[»]
1K8XX-ray1.90A1-268[»]
1K8YX-ray1.50A1-268[»]
1K8ZX-ray1.70A1-268[»]
1KFBX-ray1.90A1-268[»]
1KFCX-ray1.50A1-268[»]
1KFEX-ray1.75A1-268[»]
1KFJX-ray1.80A1-268[»]
1KFKX-ray2.40A1-268[»]
1QOPX-ray1.40A1-268[»]
1QOQX-ray1.80A1-268[»]
1TJPX-ray1.50A1-268[»]
1TTPX-ray2.30A1-268[»]
1TTQX-ray2.00A1-268[»]
1UBSX-ray1.90A1-268[»]
1WBJX-ray1.50A1-268[»]
2CLEX-ray1.50A1-268[»]
2CLFX-ray1.70A1-268[»]
2CLHX-ray1.70A1-268[»]
2CLIX-ray1.70A1-268[»]
2CLKX-ray1.50A1-268[»]
2CLLX-ray1.60A1-268[»]
2CLMX-ray1.51A1-268[»]
2CLOX-ray1.50A1-268[»]
2J9XX-ray1.90A1-268[»]
2J9YX-ray1.80A1-268[»]
2J9ZX-ray1.80A1-268[»]
2RH9X-ray1.70A1-268[»]
2RHGX-ray2.00A1-268[»]
2TRSX-ray2.04A1-268[»]
2TSYX-ray2.50A1-268[»]
2TYSX-ray1.90A1-268[»]
2WSYX-ray3.05A1-268[»]
3CEPX-ray2.10A1-268[»]
3PR2X-ray1.85A2-267[»]
4HN4X-ray1.64A1-268[»]
4HPJX-ray1.45A1-268[»]
4HPXX-ray1.65A1-268[»]
4HT3X-ray1.30A1-268[»]
4KKXX-ray1.77A1-268[»]
4WX2X-ray1.75A1-268[»]
4XUGX-ray1.65A1-268[»]
4Y6GX-ray1.65A1-268[»]
4ZQCX-ray1.54A1-268[»]
5BW6X-ray1.82A1-268[»]
5CGQX-ray1.18A1-268[»]
ProteinModelPortaliP00929
SMRiP00929
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-1033N
IntActiP00929, 1 interactor
MINTiP00929
STRINGi99287.STM1727

Chemistry databases

DrugBankiDB07748 2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL DIHYDROGEN PHOSPHATE
DB07732 2-[(2-NAPHTHYLSULFONYL)AMINO]ETHYL DIHYDROGEN PHOSPHATE
DB07745 2-{[4-(TRIFLUOROMETHOXY)BENZOYL]AMINO}ETHYL DIHYDROGEN PHOSPHATE
DB07894 4-(2-HYDROXY-4-FLUOROPHENYLTHIO)-BUTYLPHOSPHONIC ACID
DB07925 4-(2-HYDROXYPHENYLSULFINYL)-BUTYLPHOSPHONIC ACID
DB07890 4-(2-HYDROXYPHENYLTHIO)-1-BUTENYLPHOSPHONIC ACID
DB07773 5-FLUOROINDOLE PROPANOL PHOSPHATE
DB04143 Indole-3-Glycerol Phosphate
DB03171 Indole-3-Propanol Phosphate
DB07951 N-[1H-INDOL-3-YL-ACETYL]ASPARTIC ACID
DB07952 N-[1H-INDOL-3-YL-ACETYL]GLYCINE ACID
DB07953 N-[1H-INDOL-3-YL-ACETYL]VALINE ACID

Proteomic databases

PaxDbiP00929
PRIDEiP00929

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20645; AAL20645; STM1727
GeneIDi1253246
KEGGistm:STM1727
PATRICifig|99287.12.peg.1823

Phylogenomic databases

eggNOGiENOG4105F6H Bacteria
COG0159 LUCA
HOGENOMiHOG000223814
KOiK01695
OMAiDYPPEEC
PhylomeDBiP00929

Enzyme and pathway databases

UniPathwayi
UPA00035;UER00044

BioCyciSENT99287:G1FZD-1744-MONOMER

Miscellaneous databases

EvolutionaryTraceiP00929

Family and domain databases

CDDicd04724 Tryptophan_synthase_alpha, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00131 Trp_synth_alpha, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011060 RibuloseP-bd_barrel
IPR018204 Trp_synthase_alpha_AS
IPR002028 Trp_synthase_suA
PfamiView protein in Pfam
PF00290 Trp_syntA, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR00262 trpA, 1 hit
PROSITEiView protein in PROSITE
PS00167 TRP_SYNTHASE_ALPHA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA_SALTY
AccessioniPrimary (citable) accession number: P00929
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 10, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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