UniProtKB - P00919 (CAH2_RABIT)
Carbonic anhydrase 2
CA2
Functioni
Essential for bone resorption and osteoclast differentiation. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (By similarity).
Reversible hydration of carbon dioxide.
By similarityCatalytic activityi
- EC:4.2.1.1
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 7 | Fine-tunes the proton-transfer properties of H-64By similarity | 1 | |
Sitei | 62 | Fine-tunes the proton-transfer properties of H-64By similarity | 1 | |
Active sitei | 64 | Proton donor/acceptorBy similarity | 1 | |
Sitei | 67 | Fine-tunes the proton-transfer properties of H-64By similarity | 1 | |
Metal bindingi | 94 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 96 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 119 | Zinc; catalyticBy similarity | 1 |
GO - Molecular functioni
- carbonate dehydratase activity Source: UniProtKB-EC
- zinc ion binding Source: InterPro
GO - Biological processi
- angiotensin-activated signaling pathway Source: UniProtKB
- positive regulation of dipeptide transmembrane transport Source: UniProtKB
- regulation of anion transport Source: UniProtKB
- regulation of intracellular pH Source: UniProtKB
Keywordsi
Molecular function | Lyase |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Carbonic anhydrase 2 (EC:4.2.1.1)Alternative name(s): Carbonate dehydratase II Carbonic anhydrase II Short name: CA-II |
Gene namesi | Name:CA2 |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Plasma membrane
- Cell membrane By similarity
Note: Colocalized with SLC26A6 at the surface of the cell membrane in order to form a bicarbonate transport metabolon. Displaced from the cytosolic surface of the cell membrane by PKC in phorbol myristate acetate (PMA)-induced cells (By similarity).By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity1 Publication | |||
ChainiPRO_0000077420 | 2 – 260 | Carbonic anhydrase 2Add BLAST | 259 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 165 | PhosphoserineBy similarity | 1 | |
Modified residuei | 172 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinExpressioni
Gene expression databases
Bgeei | ENSOCUG00000011742, Expressed in blood and 18 other tissues |
Interactioni
Subunit structurei
Interacts with SLC4A4 and SLC26A6. Interaction with SLC4A7 regulates SLC4A7 transporter activity (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9986.ENSOCUP00000010096 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 259 | Alpha-carbonic anhydrasePROSITE-ProRule annotationAdd BLAST | 257 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 198 – 199 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0382, Eukaryota |
GeneTreei | ENSGT00940000160385 |
HOGENOMi | CLU_039326_2_1_1 |
InParanoidi | P00919 |
OMAi | DGPAHWH |
OrthoDBi | 1377476at2759 |
TreeFami | TF316425 |
Family and domain databases
Gene3Di | 3.10.200.10, 1 hit |
InterProi | View protein in InterPro IPR001148, CA_dom IPR036398, CA_dom_sf IPR023561, Carbonic_anhydrase_a-class IPR018338, Carbonic_anhydrase_a-class_CS |
PANTHERi | PTHR18952, PTHR18952, 1 hit |
Pfami | View protein in Pfam PF00194, Carb_anhydrase, 1 hit |
SMARTi | View protein in SMART SM01057, Carb_anhydrase, 1 hit |
SUPFAMi | SSF51069, SSF51069, 1 hit |
PROSITEi | View protein in PROSITE PS00162, ALPHA_CA_1, 1 hit PS51144, ALPHA_CA_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSHHWGYGKH NGPEHWHKDF PIANGERQSP IDIDTNAAKH DPSLKPLRVC
60 70 80 90 100
YEHPISRRII NNGHSFNVEF DDSHDKTVLK EGPLEGTYRL IQFHFHWGSS
110 120 130 140 150
DGQGSEHTVN KKKYAAELHL VHWNTKYGDF GKAVKHPDGL AVLGIFLKIG
160 170 180 190 200
SATPGLQKVV DTLSSIKTKG KSVDFTDFDP RGLLPESLDY WTYPGSLTTP
210 220 230 240 250
PLLECVTWIV LKEPITVSSE QMLKFRNLNF NKEAEPEEPM VDNWRPTQPL
260
KGRQVKASFV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5F9C332 | A0A5F9C332_RABIT | Carbonic anhydrase | CA2 | 247 | Annotation score: | ||
A0A5F9CGC8 | A0A5F9CGC8_RABIT | Carbonic anhydrase | CA2 | 269 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | N → D AA sequence (PubMed:416851).Curated | 1 | |
Sequence conflicti | 36 | N → D AA sequence (PubMed:416851).Curated | 1 | |
Sequence conflicti | 50 | C → S AA sequence (PubMed:416851).Curated | 1 | |
Sequence conflicti | 77 | T → S AA sequence (PubMed:416851).Curated | 1 | |
Sequence conflicti | 103 | Q → E AA sequence (PubMed:416851).Curated | 1 | |
Sequence conflicti | 177 | D → N AA sequence (PubMed:416851).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 204 | E → Q. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M98395 mRNA Translation: AAA80531.1 |
PIRi | A01142, CRRB2 |
RefSeqi | NP_001182637.1, NM_001195708.1 |
Genome annotation databases
Ensembli | ENSOCUT00000011742; ENSOCUP00000010096; ENSOCUG00000011742 |
GeneIDi | 100009156 |
KEGGi | ocu:100009156 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M98395 mRNA Translation: AAA80531.1 |
PIRi | A01142, CRRB2 |
RefSeqi | NP_001182637.1, NM_001195708.1 |
3D structure databases
SMRi | P00919 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000010096 |
Genome annotation databases
Ensembli | ENSOCUT00000011742; ENSOCUP00000010096; ENSOCUG00000011742 |
GeneIDi | 100009156 |
KEGGi | ocu:100009156 |
Organism-specific databases
CTDi | 760 |
Phylogenomic databases
eggNOGi | KOG0382, Eukaryota |
GeneTreei | ENSGT00940000160385 |
HOGENOMi | CLU_039326_2_1_1 |
InParanoidi | P00919 |
OMAi | DGPAHWH |
OrthoDBi | 1377476at2759 |
TreeFami | TF316425 |
Gene expression databases
Bgeei | ENSOCUG00000011742, Expressed in blood and 18 other tissues |
Family and domain databases
Gene3Di | 3.10.200.10, 1 hit |
InterProi | View protein in InterPro IPR001148, CA_dom IPR036398, CA_dom_sf IPR023561, Carbonic_anhydrase_a-class IPR018338, Carbonic_anhydrase_a-class_CS |
PANTHERi | PTHR18952, PTHR18952, 1 hit |
Pfami | View protein in Pfam PF00194, Carb_anhydrase, 1 hit |
SMARTi | View protein in SMART SM01057, Carb_anhydrase, 1 hit |
SUPFAMi | SSF51069, SSF51069, 1 hit |
PROSITEi | View protein in PROSITE PS00162, ALPHA_CA_1, 1 hit PS51144, ALPHA_CA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAH2_RABIT | |
Accessioni | P00919Primary (citable) accession number: P00919 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | September 29, 2021 | |
This is version 146 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families