UniProtKB - P00909 (TRPC_ECOLI)
Protein
Tryptophan biosynthesis protein TrpCF
Gene
trpC
Organism
Escherichia coli (strain K12)
Status
Functioni
Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the TrpF domain; the second reaction is catalyzed by the synthase, coded by the TrpC domain.
Catalytic activityi
- EC:5.3.1.24
- 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H+ = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2OEC:4.1.1.48
: L-tryptophan biosynthesis Pathwayi
This protein is involved in step 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (trpE), Bifunctional protein TrpGD (trpGD)
- Anthranilate phosphoribosyltransferase (trpD), Bifunctional protein TrpGD (trpGD)
- Multifunctional fusion protein (trpCF), Tryptophan biosynthesis protein TrpCF (trpC)
- Multifunctional fusion protein (trpCF), Tryptophan biosynthesis protein TrpCF (trpC)
- Tryptophan synthase alpha chain (trpA), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase beta chain (trpB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- indole-3-glycerol-phosphate synthase activity Source: EcoCyc
- phosphoribosylanthranilate isomerase activity Source: EcoCyc
GO - Biological processi
- tryptophan biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Decarboxylase, Isomerase, Lyase, Multifunctional enzyme |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:PRAI-IGPS MetaCyc:PRAI-IGPS |
BRENDAi | 5.3.1.24, 2026 |
SABIO-RKi | P00909 |
UniPathwayi | UPA00035;UER00042 UPA00035;UER00043 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:trpC Synonyms:trpF Ordered Locus Names:b1262, JW1254 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
DrugBanki | DB03543, 1-(O-Carboxy-Phenylamino)-1-Deoxy-D-Ribulose-5-Phosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154277 | 1 – 453 | Tryptophan biosynthesis protein TrpCFAdd BLAST | 453 |
Proteomic databases
jPOSTi | P00909 |
PaxDbi | P00909 |
PRIDEi | P00909 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4260122, 4 interactors 849893, 4 interactors |
IntActi | P00909, 7 interactors |
STRINGi | 511145.b1262 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P00909 |
SMRi | P00909 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00909 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 257 | Indole-3-glycerol phosphate synthaseAdd BLAST | 257 | |
Regioni | 258 – 453 | N-(5'-phosphoribosyl)anthranilate isomeraseAdd BLAST | 196 |
Sequence similaritiesi
In the N-terminal section; belongs to the TrpC family.Curated
In the C-terminal section; belongs to the TrpF family.Curated
Phylogenomic databases
eggNOGi | COG0134, Bacteria COG0135, Bacteria |
HOGENOMi | CLU_007713_1_2_6 |
InParanoidi | P00909 |
Family and domain databases
CDDi | cd00331, IGPS, 1 hit cd00405, PRAI, 1 hit |
Gene3Di | 3.20.20.70, 2 hits |
HAMAPi | MF_00134_B, IGPS_B, 1 hit MF_00135, PRAI, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR013798, Indole-3-glycerol_P_synth IPR001468, Indole-3-GlycerolPSynthase_CS IPR001240, PRAI IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00218, IGPS, 1 hit PF00697, PRAI, 1 hit |
SUPFAMi | SSF51366, SSF51366, 2 hits |
PROSITEi | View protein in PROSITE PS00614, IGPS, 1 hit |
i Sequence
Sequence statusi: Complete.
P00909-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMQTVLAKIV ADKAIWVEAR KQQQPLASFQ NEVQPSTRHF YDALQGARTA
60 70 80 90 100
FILECKKASP SKGVIRDDFD PARIAAIYKH YASAISVLTD EKYFQGSFNF
110 120 130 140 150
LPIVSQIAPQ PILCKDFIID PYQIYLARYY QADACLLMLS VLDDDQYRQL
160 170 180 190 200
AAVAHSLEMG VLTEVSNEEE QERAIALGAK VVGINNRDLR DLSIDLNRTR
210 220 230 240 250
ELAPKLGHNV TVISESGINT YAQVRELSHF ANGFLIGSAL MAHDDLHAAV
260 270 280 290 300
RRVLLGENKV CGLTRGQDAK AAYDAGAIYG GLIFVATSPR CVNVEQAQEV
310 320 330 340 350
MAAAPLQYVG VFRNHDIADV VDKAKVLSLA AVQLHGNEEQ LYIDTLREAL
360 370 380 390 400
PAHVAIWKAL SVGETLPARE FQHVDKYVLD NGQGGSGQRF DWSLLNGQSL
410 420 430 440 450
GNVLLAGGLG ADNCVEAAQT GCAGLDFNSA VESQPGIKDA RLLASVFQTL
RAY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | Missing in CAA23673 (PubMed:7038627).Curated | 1 | |
Sequence conflicti | 95 | Q → R in CAA23664 (PubMed:7007653).Curated | 1 | |
Sequence conflicti | 95 | Q → R in AAA57299 (PubMed:7007653).Curated | 1 | |
Sequence conflicti | 95 | Q → R in CAA23673 (PubMed:7038627).Curated | 1 | |
Sequence conflicti | 285 | Missing in CAA23673 (PubMed:7038627).Curated | 1 | |
Sequence conflicti | 294 | V → D in CAA23673 (PubMed:7038627).Curated | 1 | |
Sequence conflicti | 330 | A → V in CAA23664 (PubMed:7007653).Curated | 1 | |
Sequence conflicti | 330 | A → V in AAA57299 (PubMed:7007653).Curated | 1 | |
Sequence conflicti | 330 | A → V in CAA23673 (PubMed:7038627).Curated | 1 | |
Sequence conflicti | 399 | S → T in CAA23664 (PubMed:7007653).Curated | 1 | |
Sequence conflicti | 399 | S → T in AAA57299 (PubMed:7007653).Curated | 1 | |
Sequence conflicti | 399 | S → T in CAA23673 (PubMed:7038627).Curated | 1 | |
Sequence conflicti | 399 | S → T in AAB60033 (PubMed:8095913).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00366 Genomic DNA Translation: CAA23664.1 V00372 Genomic DNA Translation: CAA23673.1 J01714 Genomic DNA Translation: AAA57299.1 U23489 Genomic DNA Translation: AAB60033.1 U00096 Genomic DNA Translation: AAC74344.3 AP009048 Genomic DNA Translation: BAA14794.1 |
PIRi | A64874, GWEC |
RefSeqi | NP_415778.3, NC_000913.3 WP_000983871.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74344; AAC74344; b1262 BAA14794; BAA14794; BAA14794 |
GeneIDi | 945519 |
KEGGi | ecj:JW1254 eco:b1262 |
PATRICi | fig|511145.12.peg.1312 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00366 Genomic DNA Translation: CAA23664.1 V00372 Genomic DNA Translation: CAA23673.1 J01714 Genomic DNA Translation: AAA57299.1 U23489 Genomic DNA Translation: AAB60033.1 U00096 Genomic DNA Translation: AAC74344.3 AP009048 Genomic DNA Translation: BAA14794.1 |
PIRi | A64874, GWEC |
RefSeqi | NP_415778.3, NC_000913.3 WP_000983871.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JCM | X-ray | 2.10 | P | 2-260 | [»] | |
1PII | X-ray | 2.00 | A | 2-453 | [»] | |
2KZH | NMR | - | A | 256-385 | [»] | |
BMRBi | P00909 | |||||
SMRi | P00909 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260122, 4 interactors 849893, 4 interactors |
IntActi | P00909, 7 interactors |
STRINGi | 511145.b1262 |
Chemistry databases
DrugBanki | DB03543, 1-(O-Carboxy-Phenylamino)-1-Deoxy-D-Ribulose-5-Phosphate |
Proteomic databases
jPOSTi | P00909 |
PaxDbi | P00909 |
PRIDEi | P00909 |
Genome annotation databases
EnsemblBacteriai | AAC74344; AAC74344; b1262 BAA14794; BAA14794; BAA14794 |
GeneIDi | 945519 |
KEGGi | ecj:JW1254 eco:b1262 |
PATRICi | fig|511145.12.peg.1312 |
Organism-specific databases
EchoBASEi | EB1019 |
Phylogenomic databases
eggNOGi | COG0134, Bacteria COG0135, Bacteria |
HOGENOMi | CLU_007713_1_2_6 |
InParanoidi | P00909 |
Enzyme and pathway databases
UniPathwayi | UPA00035;UER00042 UPA00035;UER00043 |
BioCyci | EcoCyc:PRAI-IGPS MetaCyc:PRAI-IGPS |
BRENDAi | 5.3.1.24, 2026 |
SABIO-RKi | P00909 |
Miscellaneous databases
EvolutionaryTracei | P00909 |
PROi | PR:P00909 |
Family and domain databases
CDDi | cd00331, IGPS, 1 hit cd00405, PRAI, 1 hit |
Gene3Di | 3.20.20.70, 2 hits |
HAMAPi | MF_00134_B, IGPS_B, 1 hit MF_00135, PRAI, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR013798, Indole-3-glycerol_P_synth IPR001468, Indole-3-GlycerolPSynthase_CS IPR001240, PRAI IPR011060, RibuloseP-bd_barrel |
Pfami | View protein in Pfam PF00218, IGPS, 1 hit PF00697, PRAI, 1 hit |
SUPFAMi | SSF51366, SSF51366, 2 hits |
PROSITEi | View protein in PROSITE PS00614, IGPS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRPC_ECOLI | |
Accessioni | P00909Primary (citable) accession number: P00909 Secondary accession number(s): P78059, P78234, P94704 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | March 19, 2014 | |
Last modified: | April 7, 2021 | |
This is version 178 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families