UniProtKB - P00903 (PABA_ECOLI)
Protein
Aminodeoxychorismate synthase component 2
Gene
pabA
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of a heterodimeric complex that catalyzes the two-step biosynthesis of 4-amino-4-deoxychorismate (ADC), a precursor of p-aminobenzoate (PABA) and tetrahydrofolate. In the first step, a glutamine amidotransferase (PabA) generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by aminodeoxychorismate synthase (PabB) to produce ADC. PabA converts glutamine into glutamate only in the presence of stoichiometric amounts of PabB.1 Publication
Catalytic activityi
- EC:2.6.1.85
Activity regulationi
Inhibited by 6-diazo-5-oxo-L-norleucine (DON). The inhibition is competitive with glutamine, but uncompetitive with chorismate.1 Publication
Kineticsi
- KM=4.2 µM for chorismate (with the heterodimer PabA-PabB and glutamine as the amino donor at pH 7.5)1 Publication
- KM=18.6 µM for chorismate (with the heterodimer PabA-PabB and ammonia as the amino donor at pH 7.5)1 Publication
: tetrahydrofolate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 4-aminobenzoate from chorismate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Aminodeoxychorismate synthase component 1 (pabB), Aminodeoxychorismate synthase component 2 (pabA)
- Aminodeoxychorismate lyase (pabC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-aminobenzoate from chorismate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 79 | 1 Publication | 1 | |
Active sitei | 168 | 1 Publication | 1 | |
Active sitei | 170 | 1 Publication | 1 |
GO - Molecular functioni
- 4-amino-4-deoxychorismate synthase activity Source: UniProtKB
GO - Biological processi
- folic acid biosynthetic process Source: UniProtKB-KW
- glutamine metabolic process Source: UniProtKB-KW
- tetrahydrofolate biosynthetic process Source: UniProtKB
- tryptophan biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Folate biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:PABASYN-COMPII-MONOMER MetaCyc:PABASYN-COMPII-MONOMER |
SABIO-RKi | P00903 |
UniPathwayi | UPA00077;UER00149 |
Protein family/group databases
MEROPSi | C26.955 |
Names & Taxonomyi
Protein namesi | Recommended name: Aminodeoxychorismate synthase component 2 (EC:2.6.1.85)Short name: ADC synthase Short name: ADCS Alternative name(s): 4-amino-4-deoxychorismate synthase component 2 Aminodeoxychorismate synthase, glutamine amidotransferase component |
Gene namesi | Name:pabA Ordered Locus Names:b3360, JW3323 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene do not produce 4-aminobenzoate.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 79 | C → S: 10000-fold decrease in catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 168 | H → Q: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 170 | E → A: 150-fold decrease in catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 170 | E → D: 4-fold decrease in catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 170 | E → K or Q: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056849 | 1 – 187 | Aminodeoxychorismate synthase component 2Add BLAST | 187 |
Proteomic databases
jPOSTi | P00903 |
PaxDbi | P00903 |
PRIDEi | P00903 |
Interactioni
Subunit structurei
Monomer. Heterodimer consisting of two non-identical subunits: a glutamine amidotransferase subunit (PabA) and a aminodeoxychorismate synthase subunit (PabB).
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262475, 89 interactors 852184, 2 interactors |
ComplexPortali | CPX-5245, Aminodeoxychorismate synthase complex |
DIPi | DIP-10433N |
IntActi | P00903, 6 interactors |
STRINGi | 511145.b3360 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 187 | Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST | 187 |
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | COG0512, Bacteria |
HOGENOMi | CLU_014340_1_2_6 |
InParanoidi | P00903 |
PhylomeDBi | P00903 |
Family and domain databases
CDDi | cd01743, GATase1_Anthranilate_Synthase, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR017926, GATASE IPR006221, TrpG/PapA_dom |
Pfami | View protein in Pfam PF00117, GATase, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR00566, trpG_papA, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P00903-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MILLIDNYDS FTWNLYQYFC ELGADVLVKR NDALTLADID ALKPQKIVIS
60 70 80 90 100
PGPCTPDEAG ISLDVIRHYA GRLPILGVCL GHQAMAQAFG GKVVRAAKVM
110 120 130 140 150
HGKTSPITHN GEGVFRGLAN PLTVTRYHSL VVEPDSLPAC FDVTAWSETR
160 170 180
EIMGIRHRQW DLEGVQFHPE SILSEQGHQL LANFLHR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28363 Genomic DNA Translation: AAA23774.1 K00030 Genomic DNA Translation: AAA24260.1 M32354 Genomic DNA Translation: AAA24264.1 U18997 Genomic DNA Translation: AAA58157.1 U00096 Genomic DNA Translation: AAC76385.1 AP009048 Genomic DNA Translation: BAE77930.1 |
PIRi | A01124, AGEC2 |
RefSeqi | NP_417819.1, NC_000913.3 WP_000601847.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76385; AAC76385; b3360 BAE77930; BAE77930; BAE77930 |
GeneIDi | 947873 |
KEGGi | ecj:JW3323 eco:b3360 |
PATRICi | fig|1411691.4.peg.3370 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28363 Genomic DNA Translation: AAA23774.1 K00030 Genomic DNA Translation: AAA24260.1 M32354 Genomic DNA Translation: AAA24264.1 U18997 Genomic DNA Translation: AAA58157.1 U00096 Genomic DNA Translation: AAC76385.1 AP009048 Genomic DNA Translation: BAE77930.1 |
PIRi | A01124, AGEC2 |
RefSeqi | NP_417819.1, NC_000913.3 WP_000601847.1, NZ_STEB01000004.1 |
3D structure databases
SMRi | P00903 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262475, 89 interactors 852184, 2 interactors |
ComplexPortali | CPX-5245, Aminodeoxychorismate synthase complex |
DIPi | DIP-10433N |
IntActi | P00903, 6 interactors |
STRINGi | 511145.b3360 |
Protein family/group databases
MEROPSi | C26.955 |
Proteomic databases
jPOSTi | P00903 |
PaxDbi | P00903 |
PRIDEi | P00903 |
Genome annotation databases
EnsemblBacteriai | AAC76385; AAC76385; b3360 BAE77930; BAE77930; BAE77930 |
GeneIDi | 947873 |
KEGGi | ecj:JW3323 eco:b3360 |
PATRICi | fig|1411691.4.peg.3370 |
Organism-specific databases
EchoBASEi | EB0676 |
Phylogenomic databases
eggNOGi | COG0512, Bacteria |
HOGENOMi | CLU_014340_1_2_6 |
InParanoidi | P00903 |
PhylomeDBi | P00903 |
Enzyme and pathway databases
UniPathwayi | UPA00077;UER00149 |
BioCyci | EcoCyc:PABASYN-COMPII-MONOMER MetaCyc:PABASYN-COMPII-MONOMER |
SABIO-RKi | P00903 |
Miscellaneous databases
PROi | PR:P00903 |
Family and domain databases
CDDi | cd01743, GATase1_Anthranilate_Synthase, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR017926, GATASE IPR006221, TrpG/PapA_dom |
Pfami | View protein in Pfam PF00117, GATase, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR00566, trpG_papA, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PABA_ECOLI | |
Accessioni | P00903Primary (citable) accession number: P00903 Secondary accession number(s): Q2M726 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways