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Protein

Acetolactate synthase isozyme 3 small subunit

Gene

ilvH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Activity regulationi

Sensitive to valine inhibition.

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 small subunit (ilvN), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase isozyme 3 large subunit (ilvI), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 small subunit (ilvN), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase isozyme 3 large subunit (ilvI), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • acetolactate synthase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:ACETOLACTSYNIII-HCHAIN-MONOMER
MetaCyc:ACETOLACTSYNIII-HCHAIN-MONOMER
UniPathwayi
UPA00047;UER00055

UPA00049;UER00059

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase isozyme 3 small subunit (EC:2.2.1.6)
Alternative name(s):
ALS-III
Acetohydroxy-acid synthase III small subunit
Short name:
AHAS-III
Gene namesi
Name:ilvH
Synonyms:brnP
Ordered Locus Names:b0078, JW0077
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10499 ilvH

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514101 – 163Acetolactate synthase isozyme 3 small subunitAdd BLAST163

Proteomic databases

EPDiP00894
PaxDbiP00894
PRIDEiP00894

2D gel databases

SWISS-2DPAGEiP00894

Interactioni

Subunit structurei

Dimer of large and small chains.

Protein-protein interaction databases

BioGridi4259381, 5 interactors
DIPiDIP-10024N
IntActiP00894, 2 interactors
STRINGi316407.85674320

Structurei

Secondary structure

1163
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00894
SMRiP00894
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00894

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 78ACTPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108ZP8 Bacteria
COG0440 LUCA
HOGENOMiHOG000046747
InParanoidiP00894
KOiK01653
OMAiRTGKIAM
PhylomeDBiP00894

Family and domain databases

Gene3Di3.30.70.1150, 1 hit
InterProiView protein in InterPro
IPR004789 Acetalactate_synth_ssu
IPR027271 Acetolactate_synth/TF_NikR_C
IPR019455 Acetolactate_synth_ssu_C
IPR002912 ACT_dom
PANTHERiPTHR30239 PTHR30239, 1 hit
PfamiView protein in Pfam
PF10369 ALS_ss_C, 1 hit
TIGRFAMsiTIGR00119 acolac_sm, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit

Sequencei

Sequence statusi: Complete.

P00894-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRRILSVLLE NESGALSRVI GLFSQRGYNI ESLTVAPTDD PTLSRMTIQT
60 70 80 90 100
VGDEKVLEQI EKQLHKLVDV LRVSELGQGA HVEREIMLVK IQASGYGRDE
110 120 130 140 150
VKRNTEIFRG QIIDVTPSLY TVQLAGTSGK LDAFLASIRD VAKIVEVARS
160
GVVGLSRGDK IMR
Length:163
Mass (Da):17,977
Last modified:July 19, 2003 - v3
Checksum:iD6E28AE94A55606E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132D → S (PubMed:6308579).Curated1
Sequence conflicti132D → S (PubMed:1630901).Curated1
Sequence conflicti132D → S (PubMed:1851954).Curated1
Sequence conflicti159 – 163DKIMR → VK in CAA25756 (PubMed:6308579).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01609 Genomic DNA Translation: CAA25756.1
X55457 Genomic DNA No translation available.
X55034 Genomic DNA Translation: CAA38855.1
U00096 Genomic DNA Translation: AAC73189.1
AP009048 Genomic DNA Translation: BAB96647.2
M35034 Genomic DNA Translation: AAA24627.1
PIRiF64729 YCEC3H
RefSeqiNP_414620.1, NC_000913.3
WP_001300383.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73189; AAC73189; b0078
BAB96647; BAB96647; BAB96647
GeneIDi947267
KEGGiecj:JW0077
eco:b0078
PATRICifig|1411691.4.peg.2202

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01609 Genomic DNA Translation: CAA25756.1
X55457 Genomic DNA No translation available.
X55034 Genomic DNA Translation: CAA38855.1
U00096 Genomic DNA Translation: AAC73189.1
AP009048 Genomic DNA Translation: BAB96647.2
M35034 Genomic DNA Translation: AAA24627.1
PIRiF64729 YCEC3H
RefSeqiNP_414620.1, NC_000913.3
WP_001300383.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F1FX-ray1.75A/B1-163[»]
ProteinModelPortaliP00894
SMRiP00894
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259381, 5 interactors
DIPiDIP-10024N
IntActiP00894, 2 interactors
STRINGi316407.85674320

2D gel databases

SWISS-2DPAGEiP00894

Proteomic databases

EPDiP00894
PaxDbiP00894
PRIDEiP00894

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73189; AAC73189; b0078
BAB96647; BAB96647; BAB96647
GeneIDi947267
KEGGiecj:JW0077
eco:b0078
PATRICifig|1411691.4.peg.2202

Organism-specific databases

EchoBASEiEB0494
EcoGeneiEG10499 ilvH

Phylogenomic databases

eggNOGiENOG4108ZP8 Bacteria
COG0440 LUCA
HOGENOMiHOG000046747
InParanoidiP00894
KOiK01653
OMAiRTGKIAM
PhylomeDBiP00894

Enzyme and pathway databases

UniPathwayi
UPA00047;UER00055

UPA00049;UER00059

BioCyciEcoCyc:ACETOLACTSYNIII-HCHAIN-MONOMER
MetaCyc:ACETOLACTSYNIII-HCHAIN-MONOMER

Miscellaneous databases

EvolutionaryTraceiP00894
PROiPR:P00894

Family and domain databases

Gene3Di3.30.70.1150, 1 hit
InterProiView protein in InterPro
IPR004789 Acetalactate_synth_ssu
IPR027271 Acetolactate_synth/TF_NikR_C
IPR019455 Acetolactate_synth_ssu_C
IPR002912 ACT_dom
PANTHERiPTHR30239 PTHR30239, 1 hit
PfamiView protein in Pfam
PF10369 ALS_ss_C, 1 hit
TIGRFAMsiTIGR00119 acolac_sm, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiILVH_ECOLI
AccessioniPrimary (citable) accession number: P00894
Secondary accession number(s): P78046, Q47637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 19, 2003
Last modified: September 12, 2018
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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