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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive

Gene

aroF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Miscellaneous

There are 3 DAHP synthases, AroF is feedback-inhibited by Tyr. The other 2 DAHP synthases are Phe- and Trp-sensitive, respectively.

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Cofactori

Activity regulationi

Specifically feedback inhibited by tyrosine with 50% inhibition observed at 9 microM tyrosine, pH 7.0.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Quinate/shikimate dehydrogenase (ydiB)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

  • 3-deoxy-7-phosphoheptulonate synthase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: EcoCyc
  • chorismate biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:AROF-MONOMER
MetaCyc:AROF-MONOMER
UniPathwayi
UPA00053;UER00084

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:aroF
Ordered Locus Names:b2601, JW2582
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10078 aroF

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001408311 – 356Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitiveAdd BLAST356

Proteomic databases

PaxDbiP00888
PRIDEiP00888

2D gel databases

SWISS-2DPAGEiP00888

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4263470, 22 interactors
DIPiDIP-9154N
IntActiP00888, 3 interactors
STRINGi316385.ECDH10B_2768

Structurei

3D structure databases

ProteinModelPortaliP00888
SMRiP00888
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105E99 Bacteria
COG0722 LUCA
HOGENOMiHOG000220501
InParanoidiP00888
KOiK01626
PhylomeDBiP00888

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006218 DAHP1/KDSA
IPR006219 DHAP_synth_1
PANTHERiPTHR21225 PTHR21225, 1 hit
PfamiView protein in Pfam
PF00793 DAHP_synth_1, 1 hit
PIRSFiPIRSF001361 DAHP_synthase, 1 hit
TIGRFAMsiTIGR00034 aroFGH, 1 hit

Sequencei

Sequence statusi: Complete.

P00888-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQKDALNNVH ITDEQVLMTP EQLKAAFPLS LQQEAQIADS RKSISDIIAG
60 70 80 90 100
RDPRLLVVCG PCSIHDPETA LEYARRFKAL AAEVSDSLYL VMRVYFEKPR
110 120 130 140 150
TTVGWKGLIN DPHMDGSFDV EAGLQIARKL LLELVNMGLP LATEALDPNS
160 170 180 190 200
PQYLGDLFSW SAIGARTTES QTHREMASGL SMPVGFKNGT DGSLATAINA
210 220 230 240 250
MRAAAQPHRF VGINQAGQVA LLQTQGNPDG HVILRGGKAP NYSPADVAQC
260 270 280 290 300
EKEMEQAGLR PSLMVDCSHG NSNKDYRRQP AVAESVVAQI KDGNRSIIGL
310 320 330 340 350
MIESNIHEGN QSSEQPRSEM KYGVSVTDAC ISWEMTDALL REIHQDLNGQ

LTARVA
Length:356
Mass (Da):38,804
Last modified:August 13, 1987 - v1
Checksum:iA675537531FBC37D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01989 Genomic DNA Translation: AAA23489.1
M10431 Genomic DNA Translation: AAA24332.1
U00096 Genomic DNA Translation: AAC75650.1
AP009048 Genomic DNA Translation: BAA16487.1
M60890 Genomic DNA Translation: AAA23491.1
K03453 Genomic DNA Translation: AAA23490.1
PIRiI41141 ADECHY
RefSeqiNP_417092.1, NC_000913.3
WP_001168037.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75650; AAC75650; b2601
BAA16487; BAA16487; BAA16487
GeneIDi947084
KEGGiecj:JW2582
eco:b2601
PATRICifig|1411691.4.peg.4138

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01989 Genomic DNA Translation: AAA23489.1
M10431 Genomic DNA Translation: AAA24332.1
U00096 Genomic DNA Translation: AAC75650.1
AP009048 Genomic DNA Translation: BAA16487.1
M60890 Genomic DNA Translation: AAA23491.1
K03453 Genomic DNA Translation: AAA23490.1
PIRiI41141 ADECHY
RefSeqiNP_417092.1, NC_000913.3
WP_001168037.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP00888
SMRiP00888
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263470, 22 interactors
DIPiDIP-9154N
IntActiP00888, 3 interactors
STRINGi316385.ECDH10B_2768

2D gel databases

SWISS-2DPAGEiP00888

Proteomic databases

PaxDbiP00888
PRIDEiP00888

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75650; AAC75650; b2601
BAA16487; BAA16487; BAA16487
GeneIDi947084
KEGGiecj:JW2582
eco:b2601
PATRICifig|1411691.4.peg.4138

Organism-specific databases

EchoBASEiEB0076
EcoGeneiEG10078 aroF

Phylogenomic databases

eggNOGiENOG4105E99 Bacteria
COG0722 LUCA
HOGENOMiHOG000220501
InParanoidiP00888
KOiK01626
PhylomeDBiP00888

Enzyme and pathway databases

UniPathwayi
UPA00053;UER00084

BioCyciEcoCyc:AROF-MONOMER
MetaCyc:AROF-MONOMER

Miscellaneous databases

PROiPR:P00888

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006218 DAHP1/KDSA
IPR006219 DHAP_synth_1
PANTHERiPTHR21225 PTHR21225, 1 hit
PfamiView protein in Pfam
PF00793 DAHP_synth_1, 1 hit
PIRSFiPIRSF001361 DAHP_synthase, 1 hit
TIGRFAMsiTIGR00034 aroFGH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAROF_ECOLI
AccessioniPrimary (citable) accession number: P00888
Secondary accession number(s): Q47061
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: November 7, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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