aroH

Functionⁱ

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Miscellaneous

There are 3 DAHP synthases, AroH is feedback-inhibited by Trp. The other 2 DAHP synthases are Tyr- and Phe-sensitive, respectively.

Catalytic activityⁱ

Phosphoenolpyruvate + D-erythrose 4-phosphate + H₂O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Pathwayⁱ: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:

Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF)
3-dehydroquinate synthase (aroB)
3-dehydroquinate dehydratase (aroD)
Shikimate dehydrogenase (NADP(+)) (aroE), Quinate/shikimate dehydrogenase (ydiB)
Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
Chorismate synthase (aroC)

This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functionⁱ

3-deoxy-7-phosphoheptulonate synthase activity
Source: EcoliWiki
Inferred from direct assayⁱ
- 5335890
identical protein binding
Source: EcoCyc
Inferred from direct assayⁱ
- 1672127

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aromatic amino acid family biosynthetic process
Source: EcoliWiki
Inferred from direct assayⁱ
- 5335890
chorismate biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCycⁱ	EcoCyc:AROH-MONOMER MetaCyc:AROH-MONOMER
UniPathwayⁱ	UPA00053;UER00084

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (EC:2.5.1.54) Alternative name(s): 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase DAHP synthase Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesⁱ	Name:aroH Ordered Locus Names:b1704, JW1694
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10080 aroH

EcoGeneⁱ

EG10080 aroH

Subcellular locationⁱ

GO - Cellular componentⁱ

cytoplasm
Source: EcoliWiki
Inferred from direct assayⁱ
- 5335890

View the complete GO annotation on QuickGO ...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000140840	1 – 348	Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitiveAdd BLAST		348

Proteomic databases

PaxDbⁱ	P00887
PRIDEⁱ	P00887

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	3	EBI-1125143,EBI-543750

With

Entry

#Exp.

IntAct

Notes

groL

P0A6F5

3

EBI-1125143,EBI-543750

GO - Molecular functionⁱ

identical protein binding
Source: EcoCyc
Inferred from direct assayⁱ
- 1672127

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4260296, 11 interactors
Database of interacting proteins More...DIPⁱ	DIP-6846N
IntActⁱ	P00887, 5 interactors
STRINGⁱ	316385.ECDH10B_1840

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P00887
SMRⁱ	P00887
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105E99 Bacteria COG0722 LUCA
HOGENOMⁱ	HOG000220501
InParanoidⁱ	P00887
KEGG Orthology (KO) More...KOⁱ	K01626
PhylomeDBⁱ	P00887

Family and domain databases

Gene3Dⁱ	3.20.20.70, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR006218 DAHP1/KDSA IPR006219 DHAP_synth_1
PANTHERⁱ	PTHR21225 PTHR21225, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00793 DAHP_synth_1, 1 hit
PIRSFⁱ	PIRSF001361 DAHP_synthase, 1 hit
TIGRFAMsⁱ	TIGR00034 aroFGH, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P00887-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNRTDELRTA RIESLVTPAE LALRYPVTPG VATHVTDSRR RIEKILNGED 
        60         70         80         90        100
KRLLVIIGPC SIHDLTAAME YATRLQSLRN QYQSRLEIVM RTYFEKPRTV 
       110        120        130        140        150
VGWKGLISDP DLNGSYRVNH GLELARKLLL QVNELGVPTA TEFLDMVTGQ 
       160        170        180        190        200
FIADLISWGA IGARTTESQI HREMASALSC PVGFKNGTDG NTRIAVDAIR 
       210        220        230        240        250
AARASHMFLS PDKNGQMTIY QTSGNPYGHI IMRGGKKPNY HADDIAAACD 
       260        270        280        290        300
TLHEFDLPEH LVVDFSHGNC QKQHRRQLEV CEDICQQIRN GSTAIAGIMA 
       310        320        330        340 
ESFLREGTQK IVGSQPLTYG QSITDPCLGW EDTERLVEKL ASAVDTRF

Length:348

Mass (Da):38,735

Last modified:June 20, 2003 - v4

Checksum:ⁱ52C3C482447226E0

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	203 – 205	RAS → AQ in AAA23493 (PubMed:2903857).Curated	3
Sequence conflictⁱ	333 – 334	TE → RQ in AAA23493 (PubMed:2903857).Curated	2
Sequence conflictⁱ	333 – 334	TE → RQ in CAA27956 (PubMed:6167722).Curated	2
Sequence conflictⁱ	333	T → S in AAA23497 (PubMed:1677907).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M38266 Genomic DNA Translation: AAA23497.1 J04221 Genomic DNA Translation: AAA23493.1 U00096 Genomic DNA Translation: AAC74774.1 AP009048 Genomic DNA Translation: BAA15473.1 V00261 Genomic DNA Translation: CAA23510.1 X04373 Genomic DNA Translation: CAA27956.1
PIRⁱ	H64928 ADECH
NCBI Reference Sequences More...RefSeqⁱ	NP_416219.1, NC_000913.3 WP_001082229.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74774; AAC74774; b1704 BAA15473; BAA15473; BAA15473
GeneIDⁱ	946229
KEGGⁱ	ecj:JW1694 eco:b1704
PATRICⁱ	fig\|1411691.4.peg.553

Protein	Similar proteins		Species	Score	Length	Source
P00887	Phospho-2-dehydro-3-deoxyheptonate aldolase		ECOLX		389	UniRef100_P00887
Phospho-2-dehydro-3-deoxyheptonate aldolase		Escherichia coli H386		389
Phospho-2-dehydro-3-deoxyheptonate aldolase		Escherichia coli M718		389
Phospho-2-dehydro-3-deoxyheptonate aldolase		Escherichia coli H736		389
Phospho-2-dehydro-3-deoxyheptonate aldolase		Escherichia coli TA447		389
+36

Protein	Similar proteins		Species	Score	Length	Source
P00887	Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		ECOL6		348	UniRef90_P00887
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		SALTI		348
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		SHIFL		348
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		ECO57		348
Phospho-2-dehydro-3-deoxyheptonate aldolase		ECOLX		389
+784

Protein	Similar proteins		Species	Score	Length	Source
P00887	Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		ECO57		348	UniRef50_P00887
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		SALTI		348
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		SHIFL		348
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		ECOL6		348
Phospho-2-dehydro-3-deoxyheptonate aldolase		HAEIN		362
+2322

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M38266 Genomic DNA Translation: AAA23497.1 J04221 Genomic DNA Translation: AAA23493.1 U00096 Genomic DNA Translation: AAC74774.1 AP009048 Genomic DNA Translation: BAA15473.1 V00261 Genomic DNA Translation: CAA23510.1 X04373 Genomic DNA Translation: CAA27956.1
PIRⁱ	H64928 ADECH
RefSeqⁱ	NP_416219.1, NC_000913.3 WP_001082229.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P00887
SMRⁱ	P00887
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260296, 11 interactors
DIPⁱ	DIP-6846N
IntActⁱ	P00887, 5 interactors
STRINGⁱ	316385.ECDH10B_1840

Proteomic databases

PaxDbⁱ	P00887
PRIDEⁱ	P00887

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74774; AAC74774; b1704 BAA15473; BAA15473; BAA15473
GeneIDⁱ	946229
KEGGⁱ	ecj:JW1694 eco:b1704
PATRICⁱ	fig\|1411691.4.peg.553

Organism-specific databases

EchoBASEⁱ	EB0078
EcoGeneⁱ	EG10080 aroH

Phylogenomic databases

eggNOGⁱ	ENOG4105E99 Bacteria COG0722 LUCA
HOGENOMⁱ	HOG000220501
InParanoidⁱ	P00887
KOⁱ	K01626
PhylomeDBⁱ	P00887

Enzyme and pathway databases

UniPathwayⁱ	UPA00053;UER00084
BioCycⁱ	EcoCyc:AROH-MONOMER MetaCyc:AROH-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P00887

PROⁱ

PR:P00887

Family and domain databases

Gene3Dⁱ	3.20.20.70, 1 hit
InterProⁱ	View protein in InterPro IPR013785 Aldolase_TIM IPR006218 DAHP1/KDSA IPR006219 DHAP_synth_1
PANTHERⁱ	PTHR21225 PTHR21225, 1 hit
Pfamⁱ	View protein in Pfam PF00793 DAHP_synth_1, 1 hit
PIRSFⁱ	PIRSF001361 DAHP_synthase, 1 hit
TIGRFAMsⁱ	TIGR00034 aroFGH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AROH_ECOLI
Accessionⁱ	P00887Primary (citable) accession number: P00887 Secondary accession number(s): P78301
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	June 20, 2003
	Last modified:	November 7, 2018
	This is version 141 of the entry and version 4 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P00887 (AROH_ECOLI)

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Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: chorismate biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: chorismate biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ