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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive

Gene

aroH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Miscellaneous

There are 3 DAHP synthases, AroH is feedback-inhibited by Trp. The other 2 DAHP synthases are Tyr- and Phe-sensitive, respectively.

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Quinate/shikimate dehydrogenase (ydiB)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

  • 3-deoxy-7-phosphoheptulonate synthase activity Source: EcoliWiki
  • identical protein binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:AROH-MONOMER
MetaCyc:AROH-MONOMER
UniPathwayi
UPA00053;UER00084

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:aroH
Ordered Locus Names:b1704, JW1694
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10080 aroH

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001408401 – 348Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitiveAdd BLAST348

Proteomic databases

PaxDbiP00887
PRIDEiP00887

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F53EBI-1125143,EBI-543750

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4260296, 11 interactors
DIPiDIP-6846N
IntActiP00887, 5 interactors
STRINGi316385.ECDH10B_1840

Structurei

3D structure databases

ProteinModelPortaliP00887
SMRiP00887
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105E99 Bacteria
COG0722 LUCA
HOGENOMiHOG000220501
InParanoidiP00887
KOiK01626
PhylomeDBiP00887

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006218 DAHP1/KDSA
IPR006219 DHAP_synth_1
PANTHERiPTHR21225 PTHR21225, 1 hit
PfamiView protein in Pfam
PF00793 DAHP_synth_1, 1 hit
PIRSFiPIRSF001361 DAHP_synthase, 1 hit
TIGRFAMsiTIGR00034 aroFGH, 1 hit

Sequencei

Sequence statusi: Complete.

P00887-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNRTDELRTA RIESLVTPAE LALRYPVTPG VATHVTDSRR RIEKILNGED
60 70 80 90 100
KRLLVIIGPC SIHDLTAAME YATRLQSLRN QYQSRLEIVM RTYFEKPRTV
110 120 130 140 150
VGWKGLISDP DLNGSYRVNH GLELARKLLL QVNELGVPTA TEFLDMVTGQ
160 170 180 190 200
FIADLISWGA IGARTTESQI HREMASALSC PVGFKNGTDG NTRIAVDAIR
210 220 230 240 250
AARASHMFLS PDKNGQMTIY QTSGNPYGHI IMRGGKKPNY HADDIAAACD
260 270 280 290 300
TLHEFDLPEH LVVDFSHGNC QKQHRRQLEV CEDICQQIRN GSTAIAGIMA
310 320 330 340
ESFLREGTQK IVGSQPLTYG QSITDPCLGW EDTERLVEKL ASAVDTRF
Length:348
Mass (Da):38,735
Last modified:June 20, 2003 - v4
Checksum:i52C3C482447226E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203 – 205RAS → AQ in AAA23493 (PubMed:2903857).Curated3
Sequence conflicti333 – 334TE → RQ in AAA23493 (PubMed:2903857).Curated2
Sequence conflicti333 – 334TE → RQ in CAA27956 (PubMed:6167722).Curated2
Sequence conflicti333T → S in AAA23497 (PubMed:1677907).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38266 Genomic DNA Translation: AAA23497.1
J04221 Genomic DNA Translation: AAA23493.1
U00096 Genomic DNA Translation: AAC74774.1
AP009048 Genomic DNA Translation: BAA15473.1
V00261 Genomic DNA Translation: CAA23510.1
X04373 Genomic DNA Translation: CAA27956.1
PIRiH64928 ADECH
RefSeqiNP_416219.1, NC_000913.3
WP_001082229.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74774; AAC74774; b1704
BAA15473; BAA15473; BAA15473
GeneIDi946229
KEGGiecj:JW1694
eco:b1704
PATRICifig|1411691.4.peg.553

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38266 Genomic DNA Translation: AAA23497.1
J04221 Genomic DNA Translation: AAA23493.1
U00096 Genomic DNA Translation: AAC74774.1
AP009048 Genomic DNA Translation: BAA15473.1
V00261 Genomic DNA Translation: CAA23510.1
X04373 Genomic DNA Translation: CAA27956.1
PIRiH64928 ADECH
RefSeqiNP_416219.1, NC_000913.3
WP_001082229.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP00887
SMRiP00887
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260296, 11 interactors
DIPiDIP-6846N
IntActiP00887, 5 interactors
STRINGi316385.ECDH10B_1840

Proteomic databases

PaxDbiP00887
PRIDEiP00887

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74774; AAC74774; b1704
BAA15473; BAA15473; BAA15473
GeneIDi946229
KEGGiecj:JW1694
eco:b1704
PATRICifig|1411691.4.peg.553

Organism-specific databases

EchoBASEiEB0078
EcoGeneiEG10080 aroH

Phylogenomic databases

eggNOGiENOG4105E99 Bacteria
COG0722 LUCA
HOGENOMiHOG000220501
InParanoidiP00887
KOiK01626
PhylomeDBiP00887

Enzyme and pathway databases

UniPathwayi
UPA00053;UER00084

BioCyciEcoCyc:AROH-MONOMER
MetaCyc:AROH-MONOMER

Miscellaneous databases

PROiPR:P00887

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006218 DAHP1/KDSA
IPR006219 DHAP_synth_1
PANTHERiPTHR21225 PTHR21225, 1 hit
PfamiView protein in Pfam
PF00793 DAHP_synth_1, 1 hit
PIRSFiPIRSF001361 DAHP_synthase, 1 hit
TIGRFAMsiTIGR00034 aroFGH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAROH_ECOLI
AccessioniPrimary (citable) accession number: P00887
Secondary accession number(s): P78301
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 20, 2003
Last modified: November 7, 2018
This is version 141 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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