UniProtKB - P00887 (AROH_ECOLI)
Protein
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive
Gene
aroH
Organism
Escherichia coli (strain K12)
Status
Functioni
Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
Miscellaneous
There are 3 DAHP synthases, AroH is feedback-inhibited by Trp. The other 2 DAHP synthases are Tyr- and Phe-sensitive, respectively.
Catalytic activityi
- D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphateEC:2.5.1.54
: chorismate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.Proteins known to be involved in the 7 steps of the subpathway in this organism are:
- Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG)
- 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
- 3-dehydroquinate dehydratase (aroD), 3-dehydroquinate dehydratase (aroD)
- Quinate/shikimate dehydrogenase (ydiB), Quinate/shikimate dehydrogenase (ydiB), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
- Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK), Shikimate kinase 1 (aroK), Shikimate kinase 2 (aroL)
- 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
- Chorismate synthase (aroC), Chorismate synthase (aroC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.
GO - Molecular functioni
- 3-deoxy-7-phosphoheptulonate synthase activity Source: EcoliWiki
- identical protein binding Source: EcoCyc
GO - Biological processi
- aromatic amino acid family biosynthetic process Source: EcoliWiki
- cellular amino acid biosynthetic process Source: UniProtKB-KW
- chorismate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:AROH-MONOMER MetaCyc:AROH-MONOMER |
UniPathwayi | UPA00053;UER00084 |
Names & Taxonomyi
Protein namesi | Recommended name: Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (EC:2.5.1.54)Alternative name(s): 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase DAHP synthase Phospho-2-keto-3-deoxyheptonate aldolase |
Gene namesi | Name:aroH Ordered Locus Names:b1704, JW1694 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000140840 | 1 – 348 | Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitiveAdd BLAST | 348 |
Proteomic databases
jPOSTi | P00887 |
PaxDbi | P00887 |
PRIDEi | P00887 |
Interactioni
Binary interactionsi
P00887
With | #Exp. | IntAct |
---|---|---|
groL [P0A6F5] | 3 | EBI-1125143,EBI-543750 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260296, 11 interactors 850589, 1 interactor |
DIPi | DIP-6846N |
IntActi | P00887, 5 interactors |
STRINGi | 511145.b1704 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I DAHP synthase family.Curated
Phylogenomic databases
eggNOGi | COG0722, Bacteria |
HOGENOMi | CLU_030903_0_1_6 |
InParanoidi | P00887 |
PhylomeDBi | P00887 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006218, DAHP1/KDSA IPR006219, DHAP_synth_1 |
PANTHERi | PTHR21225, PTHR21225, 1 hit |
Pfami | View protein in Pfam PF00793, DAHP_synth_1, 1 hit |
PIRSFi | PIRSF001361, DAHP_synthase, 1 hit |
TIGRFAMsi | TIGR00034, aroFGH, 1 hit |
i Sequence
Sequence statusi: Complete.
P00887-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNRTDELRTA RIESLVTPAE LALRYPVTPG VATHVTDSRR RIEKILNGED
60 70 80 90 100
KRLLVIIGPC SIHDLTAAME YATRLQSLRN QYQSRLEIVM RTYFEKPRTV
110 120 130 140 150
VGWKGLISDP DLNGSYRVNH GLELARKLLL QVNELGVPTA TEFLDMVTGQ
160 170 180 190 200
FIADLISWGA IGARTTESQI HREMASALSC PVGFKNGTDG NTRIAVDAIR
210 220 230 240 250
AARASHMFLS PDKNGQMTIY QTSGNPYGHI IMRGGKKPNY HADDIAAACD
260 270 280 290 300
TLHEFDLPEH LVVDFSHGNC QKQHRRQLEV CEDICQQIRN GSTAIAGIMA
310 320 330 340
ESFLREGTQK IVGSQPLTYG QSITDPCLGW EDTERLVEKL ASAVDTRF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 203 – 205 | RAS → AQ in AAA23493 (PubMed:2903857).Curated | 3 | |
Sequence conflicti | 333 – 334 | TE → RQ in AAA23493 (PubMed:2903857).Curated | 2 | |
Sequence conflicti | 333 – 334 | TE → RQ in CAA27956 (PubMed:6167722).Curated | 2 | |
Sequence conflicti | 333 | T → S in AAA23497 (PubMed:1677907).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M38266 Genomic DNA Translation: AAA23497.1 J04221 Genomic DNA Translation: AAA23493.1 U00096 Genomic DNA Translation: AAC74774.1 AP009048 Genomic DNA Translation: BAA15473.1 V00261 Genomic DNA Translation: CAA23510.1 X04373 Genomic DNA Translation: CAA27956.1 |
PIRi | H64928, ADECH |
RefSeqi | NP_416219.1, NC_000913.3 WP_001082229.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74774; AAC74774; b1704 BAA15473; BAA15473; BAA15473 |
GeneIDi | 57731101 946229 |
KEGGi | ecj:JW1694 eco:b1704 |
PATRICi | fig|1411691.4.peg.553 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M38266 Genomic DNA Translation: AAA23497.1 J04221 Genomic DNA Translation: AAA23493.1 U00096 Genomic DNA Translation: AAC74774.1 AP009048 Genomic DNA Translation: BAA15473.1 V00261 Genomic DNA Translation: CAA23510.1 X04373 Genomic DNA Translation: CAA27956.1 |
PIRi | H64928, ADECH |
RefSeqi | NP_416219.1, NC_000913.3 WP_001082229.1, NZ_SSZK01000001.1 |
3D structure databases
SMRi | P00887 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260296, 11 interactors 850589, 1 interactor |
DIPi | DIP-6846N |
IntActi | P00887, 5 interactors |
STRINGi | 511145.b1704 |
Proteomic databases
jPOSTi | P00887 |
PaxDbi | P00887 |
PRIDEi | P00887 |
Genome annotation databases
EnsemblBacteriai | AAC74774; AAC74774; b1704 BAA15473; BAA15473; BAA15473 |
GeneIDi | 57731101 946229 |
KEGGi | ecj:JW1694 eco:b1704 |
PATRICi | fig|1411691.4.peg.553 |
Organism-specific databases
EchoBASEi | EB0078 |
Phylogenomic databases
eggNOGi | COG0722, Bacteria |
HOGENOMi | CLU_030903_0_1_6 |
InParanoidi | P00887 |
PhylomeDBi | P00887 |
Enzyme and pathway databases
UniPathwayi | UPA00053;UER00084 |
BioCyci | EcoCyc:AROH-MONOMER MetaCyc:AROH-MONOMER |
Miscellaneous databases
PROi | PR:P00887 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR006218, DAHP1/KDSA IPR006219, DHAP_synth_1 |
PANTHERi | PTHR21225, PTHR21225, 1 hit |
Pfami | View protein in Pfam PF00793, DAHP_synth_1, 1 hit |
PIRSFi | PIRSF001361, DAHP_synthase, 1 hit |
TIGRFAMsi | TIGR00034, aroFGH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AROH_ECOLI | |
Accessioni | P00887Primary (citable) accession number: P00887 Secondary accession number(s): P78301 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | June 20, 2003 | |
Last modified: | April 7, 2021 | |
This is version 158 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families