UniProtKB - P00864 (CAPP_ECOLI)
Protein
Phosphoenolpyruvate carboxylase
Gene
ppc
Organism
Escherichia coli (strain K12)
Status
Functioni
Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
Catalytic activityi
- EC:4.1.1.31
Cofactori
Mg2+By similarity
Activity regulationi
The enzyme has distinct binding sites for each of the allosteric effectors such as acetyl-CoA, fructose 1,6-bisphosphate, guanosine 3'-diphosphate 5'-diphosphate, long chain fatty acids, and L-aspartate.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 138 | 1 | ||
Active sitei | 546 | By similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: UniProtKB-UniRule
- phosphoenolpyruvate carboxylase activity Source: EcoCyc
GO - Biological processi
- carbon fixation Source: UniProtKB-UniRule
- oxaloacetate metabolic process Source: UniProtKB-UniRule
- tricarboxylic acid cycle Source: InterPro
Keywordsi
Molecular function | Allosteric enzyme, Lyase |
Biological process | Carbon dioxide fixation |
Ligand | Magnesium |
Enzyme and pathway databases
BioCyci | EcoCyc:PEPCARBOX-MONOMER MetaCyc:PEPCARBOX-MONOMER |
BRENDAi | 4.1.1.31, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ppc Synonyms:glu Ordered Locus Names:b3956, JW3928 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 138 | H → N: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 579 | H → N: 29% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 579 | H → P: 5.4% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 587 | R → S: Loss of activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB04317, 3,3-Dichloro-2-Phosphonomethyl-Acrylic Acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166592 | 1 – 883 | Phosphoenolpyruvate carboxylaseAdd BLAST | 883 |
Proteomic databases
jPOSTi | P00864 |
PaxDbi | P00864 |
PRIDEi | P00864 |
Interactioni
Subunit structurei
Homotetramer.
Protein-protein interaction databases
BioGRIDi | 4262649, 12 interactors |
DIPi | DIP-10538N |
IntActi | P00864, 3 interactors |
STRINGi | 511145.b3956 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00864 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00864 |
Family & Domainsi
Sequence similaritiesi
Belongs to the PEPCase type 1 family.Curated
Phylogenomic databases
eggNOGi | COG2352, Bacteria |
HOGENOMi | CLU_006557_2_0_6 |
InParanoidi | P00864 |
PhylomeDBi | P00864 |
Family and domain databases
HAMAPi | MF_00595, PEPcase_type1, 1 hit |
InterProi | View protein in InterPro IPR021135, PEP_COase IPR022805, PEP_COase_bac/pln-type IPR018129, PEP_COase_Lys_AS IPR033129, PEPCASE_His_AS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom |
PANTHERi | PTHR30523, PTHR30523, 1 hit |
Pfami | View protein in Pfam PF00311, PEPcase, 1 hit |
PRINTSi | PR00150, PEPCARBXLASE |
SUPFAMi | SSF51621, SSF51621, 1 hit |
PROSITEi | View protein in PROSITE PS00781, PEPCASE_1, 1 hit PS00393, PEPCASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P00864-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNEQYSALRS NVSMLGKVLG ETIKDALGEH ILERVETIRK LSKSSRAGND
60 70 80 90 100
ANRQELLTTL QNLSNDELLP VARAFSQFLN LANTAEQYHS ISPKGEAASN
110 120 130 140 150
PEVIARTLRK LKNQPELSED TIKKAVESLS LELVLTAHPT EITRRTLIHK
160 170 180 190 200
MVEVNACLKQ LDNKDIADYE HNQLMRRLRQ LIAQSWHTDE IRKLRPSPVD
210 220 230 240 250
EAKWGFAVVE NSLWQGVPNY LRELNEQLEE NLGYKLPVEF VPVRFTSWMG
260 270 280 290 300
GDRDGNPNVT ADITRHVLLL SRWKATDLFL KDIQVLVSEL SMVEATPELL
310 320 330 340 350
ALVGEEGAAE PYRYLMKNLR SRLMATQAWL EARLKGEELP KPEGLLTQNE
360 370 380 390 400
ELWEPLYACY QSLQACGMGI IANGDLLDTL RRVKCFGVPL VRIDIRQEST
410 420 430 440 450
RHTEALGELT RYLGIGDYES WSEADKQAFL IRELNSKRPL LPRNWQPSAE
460 470 480 490 500
TREVLDTCQV IAEAPQGSIA AYVISMAKTP SDVLAVHLLL KEAGIGFAMP
510 520 530 540 550
VAPLFETLDD LNNANDVMTQ LLNIDWYRGL IQGKQMVMIG YSDSAKDAGV
560 570 580 590 600
MAASWAQYQA QDALIKTCEK AGIELTLFHG RGGSIGRGGA PAHAALLSQP
610 620 630 640 650
PGSLKGGLRV TEQGEMIRFK YGLPEITVSS LSLYTGAILE ANLLPPPEPK
660 670 680 690 700
ESWRRIMDEL SVISCDVYRG YVRENKDFVP YFRSATPEQE LGKLPLGSRP
710 720 730 740 750
AKRRPTGGVE SLRAIPWIFA WTQNRLMLPA WLGAGTALQK VVEDGKQSEL
760 770 780 790 800
EAMCRDWPFF STRLGMLEMV FAKADLWLAE YYDQRLVDKA LWPLGKELRN
810 820 830 840 850
LQEEDIKVVL AIANDSHLMA DLPWIAESIQ LRNIYTDPLN VLQAELLHRS
860 870 880
RQAEKEGQEP DPRVEQALMV TIAGIAAGMR NTG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05903 Genomic DNA Translation: CAA29332.1 X55417 Genomic DNA No translation available. U00006 Genomic DNA Translation: AAC43062.1 U00096 Genomic DNA Translation: AAC76938.1 AP009048 Genomic DNA Translation: BAE77355.1 X01700 Genomic DNA Translation: CAA25847.1 |
PIRi | A01083, QYEC |
RefSeqi | NP_418391.1, NC_000913.3 WP_001005586.1, NZ_SSZK01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC76938; AAC76938; b3956 BAE77355; BAE77355; BAE77355 |
GeneIDi | 948457 |
KEGGi | ecj:JW3928 eco:b3956 |
PATRICi | fig|1411691.4.peg.2749 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Worthington enzyme manual |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05903 Genomic DNA Translation: CAA29332.1 X55417 Genomic DNA No translation available. U00006 Genomic DNA Translation: AAC43062.1 U00096 Genomic DNA Translation: AAC76938.1 AP009048 Genomic DNA Translation: BAE77355.1 X01700 Genomic DNA Translation: CAA25847.1 |
PIRi | A01083, QYEC |
RefSeqi | NP_418391.1, NC_000913.3 WP_001005586.1, NZ_SSZK01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FIY | X-ray | 2.80 | A | 1-883 | [»] | |
1JQN | X-ray | 2.35 | A | 1-883 | [»] | |
1QB4 | X-ray | 2.60 | A | 1-883 | [»] | |
SMRi | P00864 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262649, 12 interactors |
DIPi | DIP-10538N |
IntActi | P00864, 3 interactors |
STRINGi | 511145.b3956 |
Chemistry databases
DrugBanki | DB04317, 3,3-Dichloro-2-Phosphonomethyl-Acrylic Acid |
Proteomic databases
jPOSTi | P00864 |
PaxDbi | P00864 |
PRIDEi | P00864 |
Genome annotation databases
EnsemblBacteriai | AAC76938; AAC76938; b3956 BAE77355; BAE77355; BAE77355 |
GeneIDi | 948457 |
KEGGi | ecj:JW3928 eco:b3956 |
PATRICi | fig|1411691.4.peg.2749 |
Organism-specific databases
EchoBASEi | EB0749 |
Phylogenomic databases
eggNOGi | COG2352, Bacteria |
HOGENOMi | CLU_006557_2_0_6 |
InParanoidi | P00864 |
PhylomeDBi | P00864 |
Enzyme and pathway databases
BioCyci | EcoCyc:PEPCARBOX-MONOMER MetaCyc:PEPCARBOX-MONOMER |
BRENDAi | 4.1.1.31, 2026 |
Miscellaneous databases
EvolutionaryTracei | P00864 |
PROi | PR:P00864 |
Family and domain databases
HAMAPi | MF_00595, PEPcase_type1, 1 hit |
InterProi | View protein in InterPro IPR021135, PEP_COase IPR022805, PEP_COase_bac/pln-type IPR018129, PEP_COase_Lys_AS IPR033129, PEPCASE_His_AS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom |
PANTHERi | PTHR30523, PTHR30523, 1 hit |
Pfami | View protein in Pfam PF00311, PEPcase, 1 hit |
PRINTSi | PR00150, PEPCARBXLASE |
SUPFAMi | SSF51621, SSF51621, 1 hit |
PROSITEi | View protein in PROSITE PS00781, PEPCASE_1, 1 hit PS00393, PEPCASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAPP_ECOLI | |
Accessioni | P00864Primary (citable) accession number: P00864 Secondary accession number(s): Q2M8Q1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | December 2, 2020 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families