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UniProtKB - P00861 (DCDA_ECOLI)
Protein
Diaminopimelate decarboxylase
Gene
lysA
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine. Is not active against the DD- or LL-isomers of diaminopimelate.
3 PublicationsCatalytic activityi
- EC:4.1.1.203 Publications
Cofactori
pyridoxal 5'-phosphate2 Publications
Activity regulationi
Is activated by 2,3-dimercaptopropan-1-ol.1 Publication
Kineticsi
- KM=1.7 mM for meso-2,6-diaminoheptanedioate (at pH 6.8 and 37 degrees Celsius)1 Publication
- KM=1.07 mM for meso-2,6-diaminoheptanedioate (at pH 8)1 Publication
pH dependencei
Optimum pH is 6.7-6.8.1 Publication
: L-lysine biosynthesis via DAP pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-lysine from DL-2,6-diaminopimelate.1 Publication This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lysine from DL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 191 | Substrate1 Publication | 1 | |
Binding sitei | 227 | Pyridoxal phosphate; via amide nitrogen1 Publication | 1 | |
Binding sitei | 271 | Substrate1 Publication | 1 | |
Binding sitei | 307 | Substrate1 Publication | 1 | |
Binding sitei | 311 | Substrate1 Publication | 1 | |
Active sitei | 342 | Proton donorUniRule annotation | 1 | |
Binding sitei | 343 | Substrate1 Publication | 1 | |
Binding sitei | 378 | Pyridoxal phosphate1 Publication | 1 | |
Binding sitei | 378 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- diaminopimelate decarboxylase activity Source: EcoCyc
- pyridoxal phosphate binding Source: EcoCyc
GO - Biological processi
- lysine biosynthetic process via diaminopimelate Source: EcoCyc
Keywordsi
Molecular function | Decarboxylase, Lyase |
Biological process | Amino-acid biosynthesis, Lysine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:DIAMINOPIMDECARB-MONOMER |
BRENDAi | 4.1.1.20, 2026 |
SABIO-RKi | P00861 |
UniPathwayi | UPA00034;UER00027 |
Names & Taxonomyi
Protein namesi | Recommended name: Diaminopimelate decarboxylaseUniRule annotation (EC:4.1.1.20UniRule annotation3 Publications)Short name: DAP decarboxylaseUniRule annotation Short name: DAPDCUniRule annotation |
Gene namesi | Name:lysAUniRule annotation Ordered Locus Names:b2838, JW2806 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
DrugBanki | DB03814, 2-(N-morpholino)ethanesulfonic acid DB03252, D-Lysine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149923 | 1 – 420 | Diaminopimelate decarboxylaseAdd BLAST | 420 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 54 | N6-(pyridoxal phosphate)lysine1 Publication | 1 |
Proteomic databases
jPOSTi | P00861 |
PaxDbi | P00861 |
PRIDEi | P00861 |
2D gel databases
SWISS-2DPAGEi | P00861 |
Expressioni
Inductioni
Up-regulated by LysR. Repressed in the presence of lysine.2 Publications
Interactioni
Binary interactionsi
P00861
With | #Exp. | IntAct |
---|---|---|
cpxR [P0AE88] | 4 | EBI-553837,EBI-550918 |
Protein-protein interaction databases
BioGRIDi | 4261891, 8 interactors 851640, 2 interactors |
DIPi | DIP-10132N |
IntActi | P00861, 9 interactors |
STRINGi | 511145.b2838 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P00861 |
SMRi | P00861 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00861 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 268 – 271 | Pyridoxal phosphate bindingUniRule annotation1 Publication | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0019, Bacteria |
HOGENOMi | CLU_026444_0_2_6 |
InParanoidi | P00861 |
OMAi | HGNAKSP |
PhylomeDBi | P00861 |
Family and domain databases
CDDi | cd06828, PLPDE_III_DapDC, 1 hit |
Gene3Di | 2.40.37.10, 1 hit 3.20.20.10, 1 hit |
HAMAPi | MF_02120, LysA, 1 hit |
InterProi | View protein in InterPro IPR009006, Ala_racemase/Decarboxylase_C IPR002986, DAP_deCOOHase_LysA IPR022643, De-COase2_C IPR022657, De-COase2_CS IPR022644, De-COase2_N IPR022653, De-COase2_pyr-phos_BS IPR000183, Orn/DAP/Arg_de-COase IPR029066, PLP-binding_barrel |
Pfami | View protein in Pfam PF02784, Orn_Arg_deC_N, 1 hit PF00278, Orn_DAP_Arg_deC, 1 hit |
PRINTSi | PR01181, DAPDCRBXLASE PR01179, ODADCRBXLASE |
SUPFAMi | SSF50621, SSF50621, 1 hit SSF51419, SSF51419, 1 hit |
TIGRFAMsi | TIGR01048, lysA, 1 hit |
PROSITEi | View protein in PROSITE PS00878, ODR_DC_2_1, 1 hit PS00879, ODR_DC_2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P00861-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPHSLFSTDT DLTAENLLRL PAEFGCPVWV YDAQIIRRQI AALKQFDVVR
60 70 80 90 100
FAQKACSNIH ILRLMREQGV KVDSVSLGEI ERALAAGYNP QTHPDDIVFT
110 120 130 140 150
ADVIDQATLE RVSELQIPVN AGSVDMLDQL GQVSPGHRVW LRVNPGFGHG
160 170 180 190 200
HSQKTNTGGE NSKHGIWYTD LPAALDVIQR HHLQLVGIHM HIGSGVDYAH
210 220 230 240 250
LEQVCGAMVR QVIEFGQDLQ AISAGGGLSV PYQQGEEAVD TEHYYGLWNA
260 270 280 290 300
AREQIARHLG HPVKLEIEPG RFLVAQSGVL ITQVRSVKQM GSRHFVLVDA
310 320 330 340 350
GFNDLMRPAM YGSYHHISAL AADGRSLEHA PTVETVVAGP LCESGDVFTQ
360 370 380 390 400
QEGGNVETRA LPEVKAGDYL VLHDTGAYGA SMSSNYNSRP LLPEVLFDNG
410 420
QARLIRRRQT IEELLALELL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01614 Genomic DNA Translation: AAA83861.1 U29581 Genomic DNA Translation: AAB40485.1 U00096 Genomic DNA Translation: AAC75877.1 AP009048 Genomic DNA Translation: BAE76907.1 |
PIRi | A01078, DCECD |
RefSeqi | NP_417315.1, NC_000913.3 WP_001120711.1, NZ_SSUV01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC75877; AAC75877; b2838 BAE76907; BAE76907; BAE76907 |
GeneIDi | 947313 |
KEGGi | ecj:JW2806 eco:b2838 |
PATRICi | fig|1411691.4.peg.3896 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01614 Genomic DNA Translation: AAA83861.1 U29581 Genomic DNA Translation: AAB40485.1 U00096 Genomic DNA Translation: AAC75877.1 AP009048 Genomic DNA Translation: BAE76907.1 |
PIRi | A01078, DCECD |
RefSeqi | NP_417315.1, NC_000913.3 WP_001120711.1, NZ_SSUV01000026.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KNW | X-ray | 2.10 | A | 1-420 | [»] | |
1KO0 | X-ray | 2.20 | A | 1-420 | [»] | |
AlphaFoldDBi | P00861 | |||||
SMRi | P00861 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261891, 8 interactors 851640, 2 interactors |
DIPi | DIP-10132N |
IntActi | P00861, 9 interactors |
STRINGi | 511145.b2838 |
Chemistry databases
DrugBanki | DB03814, 2-(N-morpholino)ethanesulfonic acid DB03252, D-Lysine |
2D gel databases
SWISS-2DPAGEi | P00861 |
Proteomic databases
jPOSTi | P00861 |
PaxDbi | P00861 |
PRIDEi | P00861 |
Genome annotation databases
EnsemblBacteriai | AAC75877; AAC75877; b2838 BAE76907; BAE76907; BAE76907 |
GeneIDi | 947313 |
KEGGi | ecj:JW2806 eco:b2838 |
PATRICi | fig|1411691.4.peg.3896 |
Organism-specific databases
EchoBASEi | EB0544 |
Phylogenomic databases
eggNOGi | COG0019, Bacteria |
HOGENOMi | CLU_026444_0_2_6 |
InParanoidi | P00861 |
OMAi | HGNAKSP |
PhylomeDBi | P00861 |
Enzyme and pathway databases
UniPathwayi | UPA00034;UER00027 |
BioCyci | EcoCyc:DIAMINOPIMDECARB-MONOMER |
BRENDAi | 4.1.1.20, 2026 |
SABIO-RKi | P00861 |
Miscellaneous databases
EvolutionaryTracei | P00861 |
PROi | PR:P00861 |
Family and domain databases
CDDi | cd06828, PLPDE_III_DapDC, 1 hit |
Gene3Di | 2.40.37.10, 1 hit 3.20.20.10, 1 hit |
HAMAPi | MF_02120, LysA, 1 hit |
InterProi | View protein in InterPro IPR009006, Ala_racemase/Decarboxylase_C IPR002986, DAP_deCOOHase_LysA IPR022643, De-COase2_C IPR022657, De-COase2_CS IPR022644, De-COase2_N IPR022653, De-COase2_pyr-phos_BS IPR000183, Orn/DAP/Arg_de-COase IPR029066, PLP-binding_barrel |
Pfami | View protein in Pfam PF02784, Orn_Arg_deC_N, 1 hit PF00278, Orn_DAP_Arg_deC, 1 hit |
PRINTSi | PR01181, DAPDCRBXLASE PR01179, ODADCRBXLASE |
SUPFAMi | SSF50621, SSF50621, 1 hit SSF51419, SSF51419, 1 hit |
TIGRFAMsi | TIGR01048, lysA, 1 hit |
PROSITEi | View protein in PROSITE PS00878, ODR_DC_2_1, 1 hit PS00879, ODR_DC_2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DCDA_ECOLI | |
Accessioni | P00861Primary (citable) accession number: P00861 Secondary accession number(s): Q2M9Z9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | May 25, 2022 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families