UniProtKB - P00812 (ARGI_YEAST)
Protein
Arginase
Gene
CAR1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 42800 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.5.3.1By similarity
Cofactori
Mn2+PROSITE-ProRule annotationNote: Binds 2 manganese ions per subunit.PROSITE-ProRule annotation
: urea cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.By similarityProteins known to be involved in this subpathway in this organism are:
- Arginase (CAR1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 123 | Manganese 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 146 | Manganese 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 146 | Manganese 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 148 | Manganese 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 150 | Manganese 1PROSITE-ProRule annotation | 1 | |
Binding sitei | 205 | SubstrateBy similarity | 1 | |
Metal bindingi | 256 | Manganese 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 256 | Manganese 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 258 | Manganese 2PROSITE-ProRule annotation | 1 | |
Binding sitei | 270 | SubstrateBy similarity | 1 | |
Binding sitei | 301 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- arginase activity Source: SGD
- manganese ion binding Source: SGD
- ornithine carbamoyltransferase inhibitor activity Source: SGD
- zinc ion binding Source: SGD
GO - Biological processi
- arginine catabolic process to ornithine Source: SGD
- regulation of ornithine metabolic process Source: SGD
- urea cycle Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Hydrolase |
Biological process | Arginine metabolism |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:YPL111W-MONOMER |
Reactomei | R-SCE-6798695, Neutrophil degranulation R-SCE-70635, Urea cycle |
SABIO-RKi | P00812 |
UniPathwayi | UPA00158;UER00270 |
Protein family/group databases
MoonProti | P00812 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CAR1 Ordered Locus Names:YPL111W ORF Names:LPH15W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006032, CAR1 |
VEuPathDBi | FungiDB:YPL111W |
Subcellular locationi
Cytosol
- cytosol Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- mating projection tip Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173711 | 1 – 333 | ArginaseAdd BLAST | 333 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 16 | PhosphoserineCombined sources | 1 | |
Modified residuei | 77 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 270 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P00812 |
PaxDbi | P00812 |
PRIDEi | P00812 |
2D gel databases
UCD-2DPAGEi | P00812 |
PTM databases
iPTMneti | P00812 |
Expressioni
Inductioni
By arginine or homoarginine.
Interactioni
Subunit structurei
Homotrimer.
Binary interactionsi
P00812
With | #Exp. | IntAct |
---|---|---|
ARG3 [P05150] | 4 | EBI-2856,EBI-12712 |
Protein-protein interaction databases
BioGRIDi | 36070, 59 interactors |
ComplexPortali | CPX-1707, Ornithine carbamoyltransferase arginase complex |
DIPi | DIP-1224N |
IntActi | P00812, 19 interactors |
MINTi | P00812 |
STRINGi | 4932.YPL111W |
Miscellaneous databases
RNActi | P00812, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 148 – 152 | Substrate bindingBy similarity | 5 | |
Regioni | 159 – 161 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the arginase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG2965, Eukaryota |
GeneTreei | ENSGT00950000183195 |
HOGENOMi | CLU_039478_6_1_1 |
InParanoidi | P00812 |
OMAi | AHADYNT |
Family and domain databases
CDDi | cd09989, Arginase, 1 hit |
InterProi | View protein in InterPro IPR014033, Arginase IPR006035, Ureohydrolase IPR023696, Ureohydrolase_dom_sf IPR020855, Ureohydrolase_Mn_BS |
Pfami | View protein in Pfam PF00491, Arginase, 1 hit |
PRINTSi | PR00116, ARGINASE |
SUPFAMi | SSF52768, SSF52768, 1 hit |
TIGRFAMsi | TIGR01229, rocF_arginase, 1 hit |
PROSITEi | View protein in PROSITE PS01053, ARGINASE_1, 1 hit PS51409, ARGINASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P00812-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METGPHYNYY KNRELSIVLA PFSGGQGKLG VEKGPKYMLK HGLQTSIEDL
60 70 80 90 100
GWSTELEPSM DEAQFVGKLK MEKDSTTGGS SVMIDGVKAK RADLVGEATK
110 120 130 140 150
LVYNSVSKVV QANRFPLTLG GDHSIAIGTV SAVLDKYPDA GLLWIDAHAD
160 170 180 190 200
INTIESTPSG NLHGCPVSFL MGLNKDVPHC PESLKWVPGN LSPKKIAYIG
210 220 230 240 250
LRDVDAGEKK ILKDLGIAAF SMYHVDKYGI NAVIEMAMKA VHPETNGEGP
260 270 280 290 300
IMCSYDVDGV DPLYIPATGT PVRGGLTLRE GLFLVERLAE SGNLIALDVV
310 320 330
ECNPDLAIHD IHVSNTISAG CAIARCALGE TLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10414 Genomic DNA Translation: AAA34470.1 U43503 Genomic DNA Translation: AAB68250.1 M10110 Genomic DNA Translation: AAA34469.1 BK006949 Genomic DNA Translation: DAA11322.1 |
PIRi | A01008, WZBYR |
RefSeqi | NP_015214.1, NM_001183925.1 |
Genome annotation databases
EnsemblFungii | YPL111W_mRNA; YPL111W; YPL111W |
GeneIDi | 855993 |
KEGGi | sce:YPL111W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10414 Genomic DNA Translation: AAA34470.1 U43503 Genomic DNA Translation: AAB68250.1 M10110 Genomic DNA Translation: AAA34469.1 BK006949 Genomic DNA Translation: DAA11322.1 |
PIRi | A01008, WZBYR |
RefSeqi | NP_015214.1, NM_001183925.1 |
3D structure databases
SMRi | P00812 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36070, 59 interactors |
ComplexPortali | CPX-1707, Ornithine carbamoyltransferase arginase complex |
DIPi | DIP-1224N |
IntActi | P00812, 19 interactors |
MINTi | P00812 |
STRINGi | 4932.YPL111W |
Protein family/group databases
MoonProti | P00812 |
PTM databases
iPTMneti | P00812 |
2D gel databases
UCD-2DPAGEi | P00812 |
Proteomic databases
MaxQBi | P00812 |
PaxDbi | P00812 |
PRIDEi | P00812 |
Genome annotation databases
EnsemblFungii | YPL111W_mRNA; YPL111W; YPL111W |
GeneIDi | 855993 |
KEGGi | sce:YPL111W |
Organism-specific databases
SGDi | S000006032, CAR1 |
VEuPathDBi | FungiDB:YPL111W |
Phylogenomic databases
eggNOGi | KOG2965, Eukaryota |
GeneTreei | ENSGT00950000183195 |
HOGENOMi | CLU_039478_6_1_1 |
InParanoidi | P00812 |
OMAi | AHADYNT |
Enzyme and pathway databases
UniPathwayi | UPA00158;UER00270 |
BioCyci | MetaCyc:YPL111W-MONOMER |
Reactomei | R-SCE-6798695, Neutrophil degranulation R-SCE-70635, Urea cycle |
SABIO-RKi | P00812 |
Miscellaneous databases
PROi | PR:P00812 |
RNActi | P00812, protein |
Family and domain databases
CDDi | cd09989, Arginase, 1 hit |
InterProi | View protein in InterPro IPR014033, Arginase IPR006035, Ureohydrolase IPR023696, Ureohydrolase_dom_sf IPR020855, Ureohydrolase_Mn_BS |
Pfami | View protein in Pfam PF00491, Arginase, 1 hit |
PRINTSi | PR00116, ARGINASE |
SUPFAMi | SSF52768, SSF52768, 1 hit |
TIGRFAMsi | TIGR01229, rocF_arginase, 1 hit |
PROSITEi | View protein in PROSITE PS01053, ARGINASE_1, 1 hit PS51409, ARGINASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARGI_YEAST | |
Accessioni | P00812Primary (citable) accession number: P00812 Secondary accession number(s): D6W3Q6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families