ansB

Functionⁱ

Miscellaneous

E.coli contains two L-asparaginase isoenzymes: L-asparaginase I, a low-affinity enzyme located in the cytoplasm, and L-asparaginase II, a high-affinity secreted enzyme.

Catalytic activityⁱ

L-asparagine + H₂O = L-aspartate + NH₃.1 Publication

Kineticsⁱ

K_M=0.115 µM for L-asparagine

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	34	O-isoaspartyl threonine intermediatePROSITE-ProRule annotation1 Publication		1

GO - Molecular functionⁱ

asparaginase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 1821783
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 16858726
- 25040257

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

asparagine catabolic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein homotetramerization
Source: EcoCyc
Inferred from direct assayⁱ
- 8434007

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase

Molecular function

Hydrolase

Enzyme and pathway databases

BioCycⁱ	EcoCyc:ANSB-MONOMER MetaCyc:ANSB-MONOMER
BRENDAⁱ	3.5.1.1 2026
SABIO-RKⁱ	P00805

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: L-asparaginase 2 (EC:3.5.1.1) Alternative name(s): L-asparaginase II Short name: L-ASNase II L-asparagine amidohydrolase II INN: Colaspase
Gene namesⁱ	Name:ansB Ordered Locus Names:b2957, JW2924
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10046 ansB

EcoGeneⁱ

EG10046 ansB

Subcellular locationⁱ

Periplasm

GO - Cellular componentⁱ

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Periplasm

Pathology & Biotechⁱ

Pharmaceutical useⁱ

Available under the names Crastinin (Bayer), Elspar (Merck), Kidrolase (Rhone-Poulenc) and Leunase (Kyowa). Also available as a PEG-conjugated form (Pegaspargase) under the name Oncaspar (Enzon). Used as an antineoplastic in chemotherapy. Reduces the quantity of asparagine available to cancer cells.

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	34	T → A: Loss of enzyme activity. 1 Publication	1
Mutagenesisⁱ	111	T → S: Reduced enzyme activity. 1 Publication	1
Mutagenesisⁱ	111	T → V: Loss of enzyme activity. 1 Publication	1

Protein family/group databases

Allergomeⁱ	8365 Esc c Asparaginase

Allergomeⁱ

8365 Esc c Asparaginase

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB01817 Threonine-Aspartic Ester

DrugBankⁱ

DB01817 Threonine-Aspartic Ester

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 22	1 PublicationAdd BLAST		22
ChainⁱPRO_0000002356	23 – 348	L-asparaginase 2Add BLAST		326

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Disulfide bondⁱ	99 ↔ 127	2 Publications

Keywords - PTMⁱ

Disulfide bond

Proteomic databases

PaxDbⁱ	P00805
PRIDEⁱ	P00805

Expressionⁱ

Inductionⁱ

By cAMP and anaerobiosis.

Interactionⁱ

Subunit structureⁱ

Homotetramer.5 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-907458,EBI-907458

With

Entry

#Exp.

IntAct

Notes

itself

2

EBI-907458,EBI-907458

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 16858726
- 25040257

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4259243, 267 interactors
Database of interacting proteins More...DIPⁱ	DIP-9110N
IntActⁱ	P00805, 8 interactors
Molecular INTeraction database More...MINTⁱ	P00805
STRINGⁱ	316385.ECDH10B_3132

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	25 – 33	Combined sources	9
Helixⁱ	34 – 36	Combined sources	3
Beta strandⁱ	42 – 44	Combined sources	3
Beta strandⁱ	48 – 50	Combined sources	3
Helixⁱ	54 – 59	Combined sources	6
Helixⁱ	62 – 66	Combined sources	5
Beta strandⁱ	69 – 78	Combined sources	10
Helixⁱ	80 – 82	Combined sources	3
Helixⁱ	85 – 98	Combined sources	14
Helixⁱ	99 – 101	Combined sources	3
Beta strandⁱ	103 – 108	Combined sources	6
Beta strandⁱ	111 – 113	Combined sources	3
Helixⁱ	114 – 124	Combined sources	11
Beta strandⁱ	131 – 134	Combined sources	4
Helixⁱ	147 – 159	Combined sources	13
Helixⁱ	161 – 163	Combined sources	3
Beta strandⁱ	168 – 172	Combined sources	5
Beta strandⁱ	175 – 178	Combined sources	4
Turnⁱ	179 – 181	Combined sources	3
Beta strandⁱ	182 – 184	Combined sources	3
Beta strandⁱ	186 – 188	Combined sources	3
Beta strandⁱ	193 – 195	Combined sources	3
Turnⁱ	196 – 198	Combined sources	3
Beta strandⁱ	201 – 205	Combined sources	5
Beta strandⁱ	208 – 211	Combined sources	4
Helixⁱ	220 – 222	Combined sources	3
Beta strandⁱ	236 – 240	Combined sources	5
Helixⁱ	248 – 255	Combined sources	8
Beta strandⁱ	259 – 266	Combined sources	8
Turnⁱ	267 – 269	Combined sources	3
Helixⁱ	273 – 283	Combined sources	11
Turnⁱ	284 – 286	Combined sources	3
Beta strandⁱ	288 – 298	Combined sources	11
Beta strandⁱ	302 – 306	Combined sources	5
Helixⁱ	308 – 311	Combined sources	4
Beta strandⁱ	313 – 315	Combined sources	3
Helixⁱ	321 – 331	Combined sources	11
Turnⁱ	332 – 334	Combined sources	3
Helixⁱ	338 – 347	Combined sources	10

Show more details

3D structure databases

ProteinModelPortalⁱ	P00805
SMRⁱ	P00805
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P00805

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	24 – 348	Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST		325

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	80 – 81	Substrate binding		2
Regionⁱ	111 – 112	Substrate binding		2

Sequence similaritiesⁱ

Belongs to the asparaginase 1 family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG4105HDK Bacteria COG0252 LUCA
HOGENOMⁱ	HOG000044165
InParanoidⁱ	P00805
KEGG Orthology (KO) More...KOⁱ	K01424
PhylomeDBⁱ	P00805

Family and domain databases

Gene3Dⁱ	3.40.50.1170, 1 hit 3.40.50.40, 1 hit
InterProⁱ	View protein in InterPro IPR004550 AsnASE_II IPR036152 Asp/glu_Ase-like_sf IPR006034 Asparaginase/glutaminase-like IPR020827 Asparaginase/glutaminase_AS1 IPR027475 Asparaginase/glutaminase_AS2 IPR027473 L-asparaginase_C IPR027474 L-asparaginase_N IPR037152 L-asparaginase_N_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00710 Asparaginase, 1 hit
PIRSFⁱ	PIRSF001220 L-ASNase_gatD, 1 hit
PRINTSⁱ	PR00139 ASNGLNASE
SMARTⁱ	View protein in SMART SM00870 Asparaginase, 1 hit
SUPFAMⁱ	SSF53774 SSF53774, 1 hit
TIGRFAMsⁱ	TIGR00520 asnASE_II, 1 hit
PROSITEⁱ	View protein in PROSITE PS00144 ASN_GLN_ASE_1, 1 hit PS00917 ASN_GLN_ASE_2, 1 hit PS51732 ASN_GLN_ASE_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P00805-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEFFKKTALA ALVMGFSGAA LALPNITILA TGGTIAGGGD SATKSNYTVG 
        60         70         80         90        100
KVGVENLVNA VPQLKDIANV KGEQVVNIGS QDMNDNVWLT LAKKINTDCD 
       110        120        130        140        150
KTDGFVITHG TDTMEETAYF LDLTVKCDKP VVMVGAMRPS TSMSADGPFN 
       160        170        180        190        200
LYNAVVTAAD KASANRGVLV VMNDTVLDGR DVTKTNTTDV ATFKSVNYGP 
       210        220        230        240        250
LGYIHNGKID YQRTPARKHT SDTPFDVSKL NELPKVGIVY NYANASDLPA 
       260        270        280        290        300
KALVDAGYDG IVSAGVGNGN LYKSVFDTLA TAAKTGTAVV RSSRVPTGAT 
       310        320        330        340 
TQDAEVDDAK YGFVASGTLN PQKARVLLQL ALTQTKDPQQ IQQIFNQY

Length:348

Mass (Da):36,851

Last modified:November 1, 1990 - v2

Checksum:ⁱ2076987C0E8B2F99

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	49	V → A AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	86	N → D AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	132	Missing AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	156	Missing AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	171	Missing AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	206	N → D AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	268	N → D AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	274	S → T AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	285	T → D AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	290	Missing AA sequence (PubMed:6766894).Curated	1
Sequence conflictⁱ	330	Missing AA sequence (PubMed:6766894).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M34277 Genomic DNA Translation: AAA24062.1 M34234 Genomic DNA Translation: AAA23445.1 U28377 Genomic DNA Translation: AAA69124.1 U00096 Genomic DNA Translation: AAC75994.1 AP009048 Genomic DNA Translation: BAE77020.1
PIRⁱ	A35132 XDEC
NCBI Reference Sequences More...RefSeqⁱ	NP_417432.1, NC_000913.3 WP_000394140.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC75994; AAC75994; b2957 BAE77020; BAE77020; BAE77020
GeneIDⁱ	947454
KEGGⁱ	ecj:JW2924 eco:b2957
PATRICⁱ	fig\|1411691.4.peg.3775

Protein	Similar proteins		Species	Score	Length	Source
P00805	L-asparaginase		Shigella sp.		348	UniRef100_P00805
L-asparaginase, type II		ECOBD		348
L-asparaginase		ECOLX		348
UPI0000111284		ECOLX		326

Protein	Similar proteins		Species	Score	Length	Source
P00805	L-asparaginase		Shigella sp.		348	UniRef90_P00805
L-asparaginase, type II		ECOBD		348
L-asparaginase		ECOLX		348
L-asparaginase II		Escherichia fergusonii ECD227		363
L-asparaginase 2		ESCFE		348
+884

Protein	Similar proteins		Species	Score	Length	Source
P00805	L-asparaginase		Shigella sp.		348	UniRef50_P00805
L-asparaginase, type II		ECOBD		348
L-asparaginase		ECOLX		348
L-asparaginase II		Escherichia fergusonii ECD227		363
L-asparaginase 2		ESCFE		348
+1101

Cross-referencesⁱ

Web resourcesⁱ

Worthington enzyme manual

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M34277 Genomic DNA Translation: AAA24062.1 M34234 Genomic DNA Translation: AAA23445.1 U28377 Genomic DNA Translation: AAA69124.1 U00096 Genomic DNA Translation: AAC75994.1 AP009048 Genomic DNA Translation: BAE77020.1
PIRⁱ	A35132 XDEC
RefSeqⁱ	NP_417432.1, NC_000913.3 WP_000394140.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1HO3	X-ray	2.50	A/B	23-348	[»]
	1IHD	X-ray	2.65	A/C	23-348	[»]
	1JAZ	X-ray	2.27	A/B	23-348	[»]
	1JJA	X-ray	2.30	A/B/C/D/E/F	23-348	[»]
	1NNS	X-ray	1.95	A/B	23-348	[»]
	3ECA	X-ray	2.40	A/B/C/D	23-348	[»]
	4ECA	X-ray	2.20	A/B/C/D	23-348	[»]
	5MQ5	X-ray	1.60	A/B/C/D	23-348	[»]
ProteinModelPortalⁱ	P00805
SMRⁱ	P00805
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259243, 267 interactors
DIPⁱ	DIP-9110N
IntActⁱ	P00805, 8 interactors
MINTⁱ	P00805
STRINGⁱ	316385.ECDH10B_3132

Chemistry databases

DrugBankⁱ	DB01817 Threonine-Aspartic Ester

DrugBankⁱ

DB01817 Threonine-Aspartic Ester

Protein family/group databases

Allergomeⁱ	8365 Esc c Asparaginase

Allergomeⁱ

8365 Esc c Asparaginase

Proteomic databases

PaxDbⁱ	P00805
PRIDEⁱ	P00805

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC75994; AAC75994; b2957 BAE77020; BAE77020; BAE77020
GeneIDⁱ	947454
KEGGⁱ	ecj:JW2924 eco:b2957
PATRICⁱ	fig\|1411691.4.peg.3775

Organism-specific databases

EchoBASEⁱ	EB0044
EcoGeneⁱ	EG10046 ansB

Phylogenomic databases

eggNOGⁱ	ENOG4105HDK Bacteria COG0252 LUCA
HOGENOMⁱ	HOG000044165
InParanoidⁱ	P00805
KOⁱ	K01424
PhylomeDBⁱ	P00805

Enzyme and pathway databases

BioCycⁱ	EcoCyc:ANSB-MONOMER MetaCyc:ANSB-MONOMER
BRENDAⁱ	3.5.1.1 2026
SABIO-RKⁱ	P00805

Miscellaneous databases

EvolutionaryTraceⁱ	P00805
Protein Ontology More...PROⁱ	PR:P00805

Family and domain databases

Gene3Dⁱ	3.40.50.1170, 1 hit 3.40.50.40, 1 hit
InterProⁱ	View protein in InterPro IPR004550 AsnASE_II IPR036152 Asp/glu_Ase-like_sf IPR006034 Asparaginase/glutaminase-like IPR020827 Asparaginase/glutaminase_AS1 IPR027475 Asparaginase/glutaminase_AS2 IPR027473 L-asparaginase_C IPR027474 L-asparaginase_N IPR037152 L-asparaginase_N_sf
Pfamⁱ	View protein in Pfam PF00710 Asparaginase, 1 hit
PIRSFⁱ	PIRSF001220 L-ASNase_gatD, 1 hit
PRINTSⁱ	PR00139 ASNGLNASE
SMARTⁱ	View protein in SMART SM00870 Asparaginase, 1 hit
SUPFAMⁱ	SSF53774 SSF53774, 1 hit
TIGRFAMsⁱ	TIGR00520 asnASE_II, 1 hit
PROSITEⁱ	View protein in PROSITE PS00144 ASN_GLN_ASE_1, 1 hit PS00917 ASN_GLN_ASE_2, 1 hit PS51732 ASN_GLN_ASE_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ASPG2_ECOLI
Accessionⁱ	P00805Primary (citable) accession number: P00805 Secondary accession number(s): Q2M9N6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	November 1, 1990
	Last modified:	November 7, 2018
	This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

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UniProtKB - P00805 (ASPG2_ECOLI)

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L-asparaginase 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Protein family/group databases

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

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<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

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Protein-protein interaction databases

Chemistry databases

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Proteomic databases

Protocols and materials databases

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Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Kineticsⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ