UniProtKB - P00805 (ASPG2_ECOLI)
Protein
L-asparaginase 2
Gene
ansB
Organism
Escherichia coli (strain K12)
Status
Functioni
Miscellaneous
E.coli contains two L-asparaginase isoenzymes: L-asparaginase I, a low-affinity enzyme located in the cytoplasm, and L-asparaginase II, a high-affinity secreted enzyme.
Catalytic activityi
- EC:3.5.1.11 Publication
Kineticsi
- KM=0.115 µM for L-asparagine
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 34 | O-isoaspartyl threonine intermediatePROSITE-ProRule annotation1 Publication | 1 |
GO - Molecular functioni
- asparaginase activity Source: EcoCyc
- identical protein binding Source: IntAct
GO - Biological processi
- asparagine catabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BioCyci | EcoCyc:ANSB-MONOMER MetaCyc:ANSB-MONOMER |
BRENDAi | 3.5.1.1, 2026 |
SABIO-RKi | P00805 |
Names & Taxonomyi
Protein namesi | Recommended name: L-asparaginase 2 (EC:3.5.1.1)Alternative name(s): L-asparaginase II Short name: L-ASNase II L-asparagine amidohydrolase II INN: Colaspase |
Gene namesi | Name:ansB Ordered Locus Names:b2957, JW2924 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- outer membrane-bounded periplasmic space Source: EcoCyc
- periplasmic space Source: GO_Central
- protein-containing complex Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Pharmaceutical usei
Available under the names Crastinin (Bayer), Elspar (Merck), Kidrolase (Rhone-Poulenc) and Leunase (Kyowa). Also available as a PEG-conjugated form (Pegaspargase) under the name Oncaspar (Enzon). Used as an antineoplastic in chemotherapy. Reduces the quantity of asparagine available to cancer cells.
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 34 | T → A: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 111 | T → S: Reduced enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 111 | T → V: Loss of enzyme activity. 1 Publication | 1 |
Protein family/group databases
Allergomei | 8365, Esc c Asparaginase |
Chemistry databases
DrugBanki | DB01817, Threonine-Aspartic Ester |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | 1 PublicationAdd BLAST | 22 | |
ChainiPRO_0000002356 | 23 – 348 | L-asparaginase 2Add BLAST | 326 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 99 ↔ 127 | 2 Publications |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P00805 |
PaxDbi | P00805 |
PRIDEi | P00805 |
Expressioni
Inductioni
By cAMP and anaerobiosis.
Interactioni
Subunit structurei
Homotetramer.
5 PublicationsBinary interactionsi
P00805
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-907458,EBI-907458 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259243, 267 interactors 851774, 4 interactors |
DIPi | DIP-9110N |
IntActi | P00805, 8 interactors |
MINTi | P00805 |
STRINGi | 511145.b2957 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00805 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00805 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 348 | Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST | 325 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 81 | Substrate binding | 2 | |
Regioni | 111 – 112 | Substrate binding | 2 |
Sequence similaritiesi
Belongs to the asparaginase 1 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0252, Bacteria |
HOGENOMi | CLU_019134_1_2_6 |
InParanoidi | P00805 |
PhylomeDBi | P00805 |
Family and domain databases
Gene3Di | 3.40.50.1170, 1 hit 3.40.50.40, 1 hit |
InterProi | View protein in InterPro IPR004550, AsnASE_II IPR036152, Asp/glu_Ase-like_sf IPR006034, Asparaginase/glutaminase-like IPR020827, Asparaginase/glutaminase_AS1 IPR027475, Asparaginase/glutaminase_AS2 IPR040919, Asparaginase_C IPR027473, L-asparaginase_C IPR027474, L-asparaginase_N IPR037152, L-asparaginase_N_sf |
Pfami | View protein in Pfam PF00710, Asparaginase, 1 hit PF17763, Asparaginase_C, 1 hit |
PIRSFi | PIRSF001220, L-ASNase_gatD, 1 hit |
PRINTSi | PR00139, ASNGLNASE |
SMARTi | View protein in SMART SM00870, Asparaginase, 1 hit |
SUPFAMi | SSF53774, SSF53774, 1 hit |
TIGRFAMsi | TIGR00520, asnASE_II, 1 hit |
PROSITEi | View protein in PROSITE PS00144, ASN_GLN_ASE_1, 1 hit PS00917, ASN_GLN_ASE_2, 1 hit PS51732, ASN_GLN_ASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00805-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEFFKKTALA ALVMGFSGAA LALPNITILA TGGTIAGGGD SATKSNYTVG
60 70 80 90 100
KVGVENLVNA VPQLKDIANV KGEQVVNIGS QDMNDNVWLT LAKKINTDCD
110 120 130 140 150
KTDGFVITHG TDTMEETAYF LDLTVKCDKP VVMVGAMRPS TSMSADGPFN
160 170 180 190 200
LYNAVVTAAD KASANRGVLV VMNDTVLDGR DVTKTNTTDV ATFKSVNYGP
210 220 230 240 250
LGYIHNGKID YQRTPARKHT SDTPFDVSKL NELPKVGIVY NYANASDLPA
260 270 280 290 300
KALVDAGYDG IVSAGVGNGN LYKSVFDTLA TAAKTGTAVV RSSRVPTGAT
310 320 330 340
TQDAEVDDAK YGFVASGTLN PQKARVLLQL ALTQTKDPQQ IQQIFNQY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 49 | V → A AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 86 | N → D AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 132 | Missing AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 156 | Missing AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 171 | Missing AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 206 | N → D AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 268 | N → D AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 274 | S → T AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 285 | T → D AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 290 | Missing AA sequence (PubMed:6766894).Curated | 1 | |
Sequence conflicti | 330 | Missing AA sequence (PubMed:6766894).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34277 Genomic DNA Translation: AAA24062.1 M34234 Genomic DNA Translation: AAA23445.1 U28377 Genomic DNA Translation: AAA69124.1 U00096 Genomic DNA Translation: AAC75994.1 AP009048 Genomic DNA Translation: BAE77020.1 |
PIRi | A35132, XDEC |
RefSeqi | NP_417432.1, NC_000913.3 WP_000394140.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75994; AAC75994; b2957 BAE77020; BAE77020; BAE77020 |
GeneIDi | 947454 |
KEGGi | ecj:JW2924 eco:b2957 |
PATRICi | fig|1411691.4.peg.3775 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Worthington enzyme manual |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34277 Genomic DNA Translation: AAA24062.1 M34234 Genomic DNA Translation: AAA23445.1 U28377 Genomic DNA Translation: AAA69124.1 U00096 Genomic DNA Translation: AAC75994.1 AP009048 Genomic DNA Translation: BAE77020.1 |
PIRi | A35132, XDEC |
RefSeqi | NP_417432.1, NC_000913.3 WP_000394140.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HO3 | X-ray | 2.50 | A/B | 23-348 | [»] | |
1IHD | X-ray | 2.65 | A/C | 23-348 | [»] | |
1JAZ | X-ray | 2.27 | A/B | 23-348 | [»] | |
1JJA | X-ray | 2.30 | A/B/C/D/E/F | 23-348 | [»] | |
1NNS | X-ray | 1.95 | A/B | 23-348 | [»] | |
3ECA | X-ray | 2.40 | A/B/C/D | 23-348 | [»] | |
4ECA | X-ray | 2.20 | A/B/C/D | 23-348 | [»] | |
5MQ5 | X-ray | 1.60 | A/B/C/D | 23-348 | [»] | |
6EOK | X-ray | 2.50 | A/B/C/D | 23-348 | [»] | |
6NX6 | X-ray | 2.15 | A/B | 23-348 | [»] | |
6NX7 | X-ray | 2.15 | A/B | 23-348 | [»] | |
6NX8 | X-ray | 1.85 | A/B | 23-348 | [»] | |
6NX9 | X-ray | 1.97 | A/B/C/D | 23-348 | [»] | |
6NXA | X-ray | 1.93 | A/B/C/D | 23-348 | [»] | |
6NXB | X-ray | 1.75 | A/B/C/D | 23-348 | [»] | |
6PA2 | X-ray | 1.90 | A/B/C/D | 23-348 | [»] | |
6PA3 | X-ray | 1.65 | A/B/C/D | 23-348 | [»] | |
6PA4 | X-ray | 1.85 | A/B/C/D | 23-348 | [»] | |
6PA5 | X-ray | 2.00 | A/B/C/D/E/F/G/H | 23-348 | [»] | |
6PA6 | X-ray | 2.12 | A/B/C/D | 23-348 | [»] | |
6PA8 | X-ray | 1.90 | A/B/C/D | 23-348 | [»] | |
6PA9 | X-ray | 1.88 | A/B | 23-348 | [»] | |
6PAA | X-ray | 1.85 | A/B/C/D | 23-348 | [»] | |
6PAB | X-ray | 1.73 | A/B/C/D | 23-348 | [»] | |
6PAC | X-ray | 1.60 | A/B/C/D | 23-348 | [»] | |
6UOD | X-ray | 2.40 | A/B/C/D | 23-348 | [»] | |
6UOG | X-ray | 2.29 | A/B/C/D/E/F/G/H | 23-348 | [»] | |
6UOH | X-ray | 2.10 | A/B | 23-348 | [»] | |
6V23 | X-ray | 1.75 | A | 23-348 | [»] | |
6V24 | X-ray | 1.90 | A/B/C/D | 23-348 | [»] | |
6V25 | X-ray | 1.78 | A/B/C/D | 23-348 | [»] | |
6V26 | X-ray | 1.80 | A/B/C/D | 23-348 | [»] | |
6V27 | X-ray | 2.30 | A/B/C/D | 23-348 | [»] | |
6V28 | X-ray | 1.95 | A/B/C/D | 23-348 | [»] | |
6V29 | X-ray | 2.00 | A/B/C/D | 23-348 | [»] | |
6V2A | X-ray | 2.00 | A/B/C/D | 23-348 | [»] | |
6V2B | X-ray | 2.05 | A/B/C/D | 23-348 | [»] | |
6V2C | X-ray | 2.00 | A/B/C/D | 23-348 | [»] | |
6V2G | X-ray | 1.90 | A/B/C/D | 23-348 | [»] | |
SMRi | P00805 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259243, 267 interactors 851774, 4 interactors |
DIPi | DIP-9110N |
IntActi | P00805, 8 interactors |
MINTi | P00805 |
STRINGi | 511145.b2957 |
Chemistry databases
DrugBanki | DB01817, Threonine-Aspartic Ester |
Protein family/group databases
Allergomei | 8365, Esc c Asparaginase |
Proteomic databases
jPOSTi | P00805 |
PaxDbi | P00805 |
PRIDEi | P00805 |
Genome annotation databases
EnsemblBacteriai | AAC75994; AAC75994; b2957 BAE77020; BAE77020; BAE77020 |
GeneIDi | 947454 |
KEGGi | ecj:JW2924 eco:b2957 |
PATRICi | fig|1411691.4.peg.3775 |
Organism-specific databases
EchoBASEi | EB0044 |
Phylogenomic databases
eggNOGi | COG0252, Bacteria |
HOGENOMi | CLU_019134_1_2_6 |
InParanoidi | P00805 |
PhylomeDBi | P00805 |
Enzyme and pathway databases
BioCyci | EcoCyc:ANSB-MONOMER MetaCyc:ANSB-MONOMER |
BRENDAi | 3.5.1.1, 2026 |
SABIO-RKi | P00805 |
Miscellaneous databases
EvolutionaryTracei | P00805 |
PROi | PR:P00805 |
Family and domain databases
Gene3Di | 3.40.50.1170, 1 hit 3.40.50.40, 1 hit |
InterProi | View protein in InterPro IPR004550, AsnASE_II IPR036152, Asp/glu_Ase-like_sf IPR006034, Asparaginase/glutaminase-like IPR020827, Asparaginase/glutaminase_AS1 IPR027475, Asparaginase/glutaminase_AS2 IPR040919, Asparaginase_C IPR027473, L-asparaginase_C IPR027474, L-asparaginase_N IPR037152, L-asparaginase_N_sf |
Pfami | View protein in Pfam PF00710, Asparaginase, 1 hit PF17763, Asparaginase_C, 1 hit |
PIRSFi | PIRSF001220, L-ASNase_gatD, 1 hit |
PRINTSi | PR00139, ASNGLNASE |
SMARTi | View protein in SMART SM00870, Asparaginase, 1 hit |
SUPFAMi | SSF53774, SSF53774, 1 hit |
TIGRFAMsi | TIGR00520, asnASE_II, 1 hit |
PROSITEi | View protein in PROSITE PS00144, ASN_GLN_ASE_1, 1 hit PS00917, ASN_GLN_ASE_2, 1 hit PS51732, ASN_GLN_ASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASPG2_ECOLI | |
Accessioni | P00805Primary (citable) accession number: P00805 Secondary accession number(s): Q2M9N6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | November 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 188 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Pharmaceutical, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families