UniProtKB - P00804 (LSPA_ECOLI)
Protein
Lipoprotein signal peptidase
Gene
lspA
Organism
Escherichia coli (strain K12)
Status
Functioni
This protein specifically catalyzes the removal of signal peptides from prolipoproteins.3 Publications
Catalytic activityi
- Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.1 Publication EC:3.4.23.36
Activity regulationi
Enzyme activity is inhibited by globomycin, a cyclic peptide antibiotic.1 Publication
pH dependencei
Optimum pH is 7.9.1 Publication
Temperature dependencei
Optimum temperature is 37-45 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 114 | By similarity | 1 | |
Active sitei | 141 | By similarity | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-UniRule
- endopeptidase activity Source: EcoCyc
GO - Biological processi
- lipoprotein biosynthetic process via signal peptide cleavage Source: EcoCyc
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10548-MONOMER MetaCyc:EG10548-MONOMER |
Protein family/group databases
MEROPSi | A08.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Lipoprotein signal peptidase (EC:3.4.23.36)Alternative name(s): Prolipoprotein signal peptidase Signal peptidase II Short name: SPase II |
Gene namesi | Name:lspA Synonyms:lsp Ordered Locus Names:b0027, JW0025 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicCuratedAdd BLAST | 11 | |
Transmembranei | 12 – 26 | HelicalCuratedAdd BLAST | 15 | |
Topological domaini | 27 – 69 | PeriplasmicCuratedAdd BLAST | 43 | |
Transmembranei | 70 – 88 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 89 – 95 | CytoplasmicCurated | 7 | |
Transmembranei | 96 – 113 | HelicalCuratedAdd BLAST | 18 | |
Topological domaini | 114 – 141 | PeriplasmicCuratedAdd BLAST | 28 | |
Transmembranei | 142 – 159 | HelicalCuratedAdd BLAST | 18 | |
Topological domaini | 160 – 164 | CytoplasmicCurated | 5 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000178778 | 1 – 164 | Lipoprotein signal peptidaseAdd BLAST | 164 |
Proteomic databases
PaxDbi | P00804 |
PRIDEi | P00804 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259402, 214 interactors |
DIPi | DIP-10129N |
STRINGi | 511145.b0027 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase A8 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0597, Bacteria |
HOGENOMi | CLU_083252_4_0_6 |
InParanoidi | P00804 |
PhylomeDBi | P00804 |
Family and domain databases
HAMAPi | MF_00161, LspA, 1 hit |
InterProi | View protein in InterPro IPR001872, Peptidase_A8 |
PANTHERi | PTHR33695, PTHR33695, 1 hit |
Pfami | View protein in Pfam PF01252, Peptidase_A8, 1 hit |
PRINTSi | PR00781, LIPOSIGPTASE |
TIGRFAMsi | TIGR00077, lspA, 1 hit |
PROSITEi | View protein in PROSITE PS00855, SPASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
P00804-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQSICSTGL RWLWLVVVVL IIDLGSKYLI LQNFALGDTV PLFPSLNLHY
60 70 80 90 100
ARNYGAAFSF LADSGGWQRW FFAGIAIGIS VILAVMMYRS KATQKLNNIA
110 120 130 140 150
YALIIGGALG NLFDRLWHGF VVDMIDFYVG DWHFATFNLA DTAICVGAAL
160
IVLEGFLPSR AKKQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00776 Genomic DNA Translation: CAA25353.1 K01990 Genomic DNA Translation: AAA24092.1 U00096 Genomic DNA Translation: AAC73138.1 AP009048 Genomic DNA Translation: BAB96596.1 X54945 Genomic DNA Translation: CAA38705.1 |
PIRi | C64723, ZPECL |
RefSeqi | NP_414568.1, NC_000913.3 WP_000083372.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73138; AAC73138; b0027 BAB96596; BAB96596; BAB96596 |
GeneIDi | 944800 |
KEGGi | ecj:JW0025 eco:b0027 |
PATRICi | fig|1411691.4.peg.2258 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00776 Genomic DNA Translation: CAA25353.1 K01990 Genomic DNA Translation: AAA24092.1 U00096 Genomic DNA Translation: AAC73138.1 AP009048 Genomic DNA Translation: BAB96596.1 X54945 Genomic DNA Translation: CAA38705.1 |
PIRi | C64723, ZPECL |
RefSeqi | NP_414568.1, NC_000913.3 WP_000083372.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4259402, 214 interactors |
DIPi | DIP-10129N |
STRINGi | 511145.b0027 |
Protein family/group databases
MEROPSi | A08.001 |
Proteomic databases
PaxDbi | P00804 |
PRIDEi | P00804 |
Genome annotation databases
EnsemblBacteriai | AAC73138; AAC73138; b0027 BAB96596; BAB96596; BAB96596 |
GeneIDi | 944800 |
KEGGi | ecj:JW0025 eco:b0027 |
PATRICi | fig|1411691.4.peg.2258 |
Organism-specific databases
EchoBASEi | EB0543 |
Phylogenomic databases
eggNOGi | COG0597, Bacteria |
HOGENOMi | CLU_083252_4_0_6 |
InParanoidi | P00804 |
PhylomeDBi | P00804 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10548-MONOMER MetaCyc:EG10548-MONOMER |
Miscellaneous databases
PROi | PR:P00804 |
Family and domain databases
HAMAPi | MF_00161, LspA, 1 hit |
InterProi | View protein in InterPro IPR001872, Peptidase_A8 |
PANTHERi | PTHR33695, PTHR33695, 1 hit |
Pfami | View protein in Pfam PF01252, Peptidase_A8, 1 hit |
PRINTSi | PR00781, LIPOSIGPTASE |
TIGRFAMsi | TIGR00077, lspA, 1 hit |
PROSITEi | View protein in PROSITE PS00855, SPASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LSPA_ECOLI | |
Accessioni | P00804Primary (citable) accession number: P00804 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | August 13, 1987 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families