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UniProtKB - P00803 (LEP_ECOLI)
Protein
Signal peptidase I
Gene
lepB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins. EC:3.4.21.89
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 91 | 1 | ||
Active sitei | 146 | 1 |
GO - Molecular functioni
- endopeptidase activity Source: EcoCyc
- peptidase activity Source: EcoliWiki
- serine-type endopeptidase activity Source: EcoCyc
- toxic substance binding Source: EcoliWiki
GO - Biological processi
- protein processing Source: EcoliWiki
- proteolysis Source: EcoliWiki
- signal peptide processing Source: EcoCyc
Keywordsi
Molecular function | Hydrolase, Protease |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10530-MONOMER |
BRENDAi | 3.4.21.89, 2026 |
SABIO-RKi | P00803 |
Protein family/group databases
MEROPSi | S26.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Signal peptidase I (EC:3.4.21.89)Short name: SPase I Alternative name(s): Leader peptidase I |
Gene namesi | Name:lepB Ordered Locus Names:b2568, JW2552 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoliWiki
Other locations
- integral component of membrane Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 3 | PeriplasmicCurated | 3 | |
Transmembranei | 4 – 22 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 23 – 58 | CytoplasmicCuratedAdd BLAST | 36 | |
Transmembranei | 59 – 77 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 78 – 324 | PeriplasmicCuratedAdd BLAST | 247 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 62 | E → V: Indifferent. | 1 | |
Mutagenesisi | 78 | R → E, N or L: Indifferent. | 1 | |
Mutagenesisi | 91 | S → A: Loss of activity. | 1 | |
Mutagenesisi | 125 | H → N: Indifferent. | 1 | |
Mutagenesisi | 128 | R → Q: Small effect. | 1 | |
Mutagenesisi | 130 | D → A: Indifferent. | 1 | |
Mutagenesisi | 144 | Y → F: Indifferent. | 1 | |
Mutagenesisi | 146 | K → M, D, G or S: Loss of activity. | 1 | |
Mutagenesisi | 147 | R → Q: Small effect. | 1 | |
Mutagenesisi | 154 | D → A: Loss of activity. | 1 | |
Mutagenesisi | 154 | D → N: Indifferent. | 1 | |
Mutagenesisi | 159 | D → N: Small effect. | 1 | |
Mutagenesisi | 171 | C → A: Indifferent. | 1 | |
Mutagenesisi | 177 | C → A: Indifferent. | 1 | |
Mutagenesisi | 236 | H → N: Indifferent. | 1 | |
Mutagenesisi | 269 | Y → F: Indifferent. | 1 | |
Mutagenesisi | 274 | D → A: Indifferent. | 1 | |
Mutagenesisi | 276 | R → Q: Small effect. | 1 | |
Mutagenesisi | 281 | D → A: Indifferent. | 1 | |
Mutagenesisi | 283 | R → Q: Small effect. | 1 |
Chemistry databases
ChEMBLi | CHEMBL4470 |
DrugBanki | DB06904, (5S,6S)-6-[(R)ACETOXYETH-2-YL]-PENEM-3-CARBOXYLATEPROPANE DB02080, 1-{2-[2-(2-Methoxyethoxy)Ethoxy]Ethoxy}-4-(1,1,3,3-Tetramethylbutyl)Benzene DB01934, Arylomycin A2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109506 | 1 – 324 | Signal peptidase IAdd BLAST | 324 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | Blocked amino end (Met)1 Publication | 1 | |
Disulfide bondi | 171 ↔ 177 |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P00803 |
PaxDbi | P00803 |
PRIDEi | P00803 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259467, 112 interactors |
STRINGi | 511145.b2568 |
Chemistry databases
BindingDBi | P00803 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00803 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00803 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S26 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0681, Bacteria |
HOGENOMi | CLU_028723_1_1_6 |
InParanoidi | P00803 |
PhylomeDBi | P00803 |
Family and domain databases
CDDi | cd06530, S26_SPase_I, 1 hit |
Gene3Di | 2.170.230.10, 1 hit |
InterProi | View protein in InterPro IPR036286, LexA/Signal_pep-like_sf IPR000223, Pept_S26A_signal_pept_1 IPR019758, Pept_S26A_signal_pept_1_CS IPR019757, Pept_S26A_signal_pept_1_Lys-AS IPR019756, Pept_S26A_signal_pept_1_Ser-AS IPR019533, Peptidase_S26 IPR019766, Sign_pep_all-beta_subdom |
PANTHERi | PTHR43390, PTHR43390, 1 hit |
Pfami | View protein in Pfam PF10502, Peptidase_S26, 1 hit |
PRINTSi | PR00727, LEADERPTASE |
SUPFAMi | SSF51306, SSF51306, 1 hit |
TIGRFAMsi | TIGR02227, sigpep_I_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00501, SPASE_I_1, 1 hit PS00760, SPASE_I_2, 1 hit PS00761, SPASE_I_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P00803-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANMFALILV IATLVTGILW CVDKFFFAPK RRERQAAAQA AAGDSLDKAT
60 70 80 90 100
LKKVAPKPGW LETGASVFPV LAIVLIVRSF IYEPFQIPSG SMMPTLLIGD
110 120 130 140 150
FILVEKFAYG IKDPIYQKTL IETGHPKRGD IVVFKYPEDP KLDYIKRAVG
160 170 180 190 200
LPGDKVTYDP VSKELTIQPG CSSGQACENA LPVTYSNVEP SDFVQTFSRR
210 220 230 240 250
NGGEATSGFF EVPKNETKEN GIRLSERKET LGDVTHRILT VPIAQDQVGM
260 270 280 290 300
YYQQPGQQLA TWIVPPGQYF MMGDNRDNSA DSRYWGFVPE ANLVGRATAI
310 320
WMSFDKQEGE WPTGLRLSRI GGIH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 – 43 | AG → R (PubMed:6311837).Curated | 2 | |
Sequence conflicti | 42 – 43 | AG → R (Ref. 2) Curated | 2 | |
Sequence conflicti | 123 | T → N (PubMed:6311837).Curated | 1 | |
Sequence conflicti | 123 | T → N (Ref. 2) Curated | 1 | |
Sequence conflicti | 183 | V → A (PubMed:6311837).Curated | 1 | |
Sequence conflicti | 183 | V → A (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K00426 Genomic DNA Translation: AAA24064.1 D64044 Genomic DNA Translation: BAA10915.1 U00096 Genomic DNA Translation: AAC75621.1 AP009048 Genomic DNA Translation: BAE76744.1 |
PIRi | G65034, ZPECS |
RefSeqi | NP_417063.1, NC_000913.3 WP_000002541.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75621; AAC75621; b2568 BAE76744; BAE76744; BAE76744 |
GeneIDi | 947040 |
KEGGi | ecj:JW2552 eco:b2568 |
PATRICi | fig|1411691.4.peg.4166 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K00426 Genomic DNA Translation: AAA24064.1 D64044 Genomic DNA Translation: BAA10915.1 U00096 Genomic DNA Translation: AAC75621.1 AP009048 Genomic DNA Translation: BAE76744.1 |
PIRi | G65034, ZPECS |
RefSeqi | NP_417063.1, NC_000913.3 WP_000002541.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B12 | X-ray | 1.95 | A/B/C/D | 77-324 | [»] | |
1KN9 | X-ray | 2.40 | A/B/C/D | 77-324 | [»] | |
1T7D | X-ray | 2.47 | A/B | 76-324 | [»] | |
3IIQ | X-ray | 2.00 | A/B | 77-324 | [»] | |
3S04 | X-ray | 2.44 | A/B | 76-324 | [»] | |
6B88 | X-ray | 2.41 | A/B | 78-324 | [»] | |
SMRi | P00803 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259467, 112 interactors |
STRINGi | 511145.b2568 |
Chemistry databases
BindingDBi | P00803 |
ChEMBLi | CHEMBL4470 |
DrugBanki | DB06904, (5S,6S)-6-[(R)ACETOXYETH-2-YL]-PENEM-3-CARBOXYLATEPROPANE DB02080, 1-{2-[2-(2-Methoxyethoxy)Ethoxy]Ethoxy}-4-(1,1,3,3-Tetramethylbutyl)Benzene DB01934, Arylomycin A2 |
Protein family/group databases
MEROPSi | S26.001 |
Proteomic databases
jPOSTi | P00803 |
PaxDbi | P00803 |
PRIDEi | P00803 |
Genome annotation databases
EnsemblBacteriai | AAC75621; AAC75621; b2568 BAE76744; BAE76744; BAE76744 |
GeneIDi | 947040 |
KEGGi | ecj:JW2552 eco:b2568 |
PATRICi | fig|1411691.4.peg.4166 |
Organism-specific databases
EchoBASEi | EB0525 |
Phylogenomic databases
eggNOGi | COG0681, Bacteria |
HOGENOMi | CLU_028723_1_1_6 |
InParanoidi | P00803 |
PhylomeDBi | P00803 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10530-MONOMER |
BRENDAi | 3.4.21.89, 2026 |
SABIO-RKi | P00803 |
Miscellaneous databases
EvolutionaryTracei | P00803 |
PROi | PR:P00803 |
Family and domain databases
CDDi | cd06530, S26_SPase_I, 1 hit |
Gene3Di | 2.170.230.10, 1 hit |
InterProi | View protein in InterPro IPR036286, LexA/Signal_pep-like_sf IPR000223, Pept_S26A_signal_pept_1 IPR019758, Pept_S26A_signal_pept_1_CS IPR019757, Pept_S26A_signal_pept_1_Lys-AS IPR019756, Pept_S26A_signal_pept_1_Ser-AS IPR019533, Peptidase_S26 IPR019766, Sign_pep_all-beta_subdom |
PANTHERi | PTHR43390, PTHR43390, 1 hit |
Pfami | View protein in Pfam PF10502, Peptidase_S26, 1 hit |
PRINTSi | PR00727, LEADERPTASE |
SUPFAMi | SSF51306, SSF51306, 1 hit |
TIGRFAMsi | TIGR02227, sigpep_I_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00501, SPASE_I_1, 1 hit PS00760, SPASE_I_2, 1 hit PS00761, SPASE_I_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LEP_ECOLI | |
Accessioni | P00803Primary (citable) accession number: P00803 Secondary accession number(s): P78098, Q2MAG2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families