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Protein

Thermolysin

Gene

npr

Organism
Bacillus thermoproteolyticus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 4 Ca2+ ions per subunit.
  • Zn2+Note: Binds 1 zinc ion per subunit.

Temperature dependencei

Thermostable.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi289Calcium 11
Metal bindingi291Calcium 11
Metal bindingi293Calcium 1; via carbonyl oxygen1
Metal bindingi370Calcium 21
Metal bindingi374Zinc; catalytic1
Active sitei375PROSITE-ProRule annotation1 Publication1
Metal bindingi378Zinc; catalytic1
Metal bindingi398Zinc; catalytic1
Metal bindingi409Calcium 21
Metal bindingi409Calcium 31
Metal bindingi415Calcium 3; via carbonyl oxygen1
Metal bindingi417Calcium 21
Metal bindingi417Calcium 31
Metal bindingi419Calcium 2; via carbonyl oxygen1
Metal bindingi422Calcium 21
Metal bindingi422Calcium 31
Metal bindingi425Calcium 4; via carbonyl oxygen1
Metal bindingi426Calcium 41
Metal bindingi429Calcium 4; via carbonyl oxygen1
Metal bindingi432Calcium 41
Active sitei463Proton donorPROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.27 708
SABIO-RKiP00800

Protein family/group databases

MEROPSiM04.001

Names & Taxonomyi

Protein namesi
Recommended name:
Thermolysin (EC:3.4.24.27)
Alternative name(s):
Thermostable neutral proteinase
Gene namesi
Name:npr
OrganismiBacillus thermoproteolyticus
Taxonomic identifieri1427 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3392
DrugBankiDB07103 2-(4-METHYLPHENOXY)ETHYLPHOSPHINATE
DB07989 2-(ACETYL-HYDROXY-AMINO)-4-METHYL-PENTANOIC ACID METHYL ESTER
DB01935 3-{[(1r)-1-Benzyl-2-Sulfanylethyl]Amino}-3-Oxopropanoic Acid
DB07673 DEAMINO-METHYL-PHENYLALANINE
DB02000 Gamma-Phenyl-Butyric Acid
DB07434 HONH-BENZYLMALONYL-L-ALANYLGLYCINE-P-NITROANILIDE
DB07506 L-BENZYLSUCCINIC ACID
DB02669 RB106

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28By similarityAdd BLAST28
PropeptideiPRO_000002859229 – 232Activation peptide2 PublicationsAdd BLAST204
ChainiPRO_0000028593233 – 548ThermolysinAdd BLAST316

Keywords - PTMi

Zymogen

Interactioni

Chemistry databases

BindingDBiP00800

Structurei

Secondary structure

1548
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00800
SMRiP00800
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00800

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd09597 M4_neutral_protease, 1 hit
Gene3Di1.10.390.10, 1 hit
InterProiView protein in InterPro
IPR011096 FTP_domain
IPR025711 PepSY
IPR023612 Peptidase_M4
IPR027268 Peptidase_M4/M1_CTD_sf
IPR001570 Peptidase_M4_C_domain
IPR013856 Peptidase_M4_domain
PfamiView protein in Pfam
PF07504 FTP, 1 hit
PF03413 PepSY, 1 hit
PF01447 Peptidase_M4, 1 hit
PF02868 Peptidase_M4_C, 1 hit
PRINTSiPR00730 THERMOLYSIN
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00800-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKMKMKLASF GLAAGLAAQV FLPYNALAST EHVTWNQQFQ TPQFISGDLL
60 70 80 90 100
KVNGTSPEEL VYQYVEKNEN KFKFHENAKD TLQLKEKKND NLGFTFMRFQ
110 120 130 140 150
QTYKGIPVFG AVVTSHVKDG TLTALSGTLI PNLDTKGSLK SGKKLSEKQA
160 170 180 190 200
RDIAEKDLVA NVTKEVPEYE QGKDTEFVVY VNGDEASLAY VVNLNFLTPE
210 220 230 240 250
PGNWLYIIDA VDGKILNKFN QLDAAKPGDV KSITGTSTVG VGRGVLGDQK
260 270 280 290 300
NINTTYSTYY YLQDNTRGNG IFTYDAKYRT TLPGSLWADA DNQFFASYDA
310 320 330 340 350
PAVDAHYYAG VTYDYYKNVH NRLSYDGNNA AIRSSVHYSQ GYNNAFWNGS
360 370 380 390 400
QMVYGDGDGQ TFIPLSGGID VVAHELTHAV TDYTAGLIYQ NESGAINEAI
410 420 430 440 450
SDIFGTLVEF YANKNPDWEI GEDVYTPGIS GDSLRSMSDP AKYGDPDHYS
460 470 480 490 500
KRYTGTQDNG GVHINSGIIN KAAYLISQGG THYGVSVVGI GRDKLGKIFY
510 520 530 540
RALTQYLTPT SNFSQLRAAA VQSATDLYGS TSQEVASVKQ AFDAVGVK
Length:548
Mass (Da):60,104
Last modified:April 18, 2006 - v3
Checksum:i1D6ED3A545F045C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti269N → D AA sequence (Ref. 2) Curated1
Sequence conflicti351Q → E AA sequence (Ref. 2) Curated1
Sequence conflicti400I → M in CAA54291 (PubMed:8002967).Curated1
Sequence conflicti409E → K in CAA54291 (PubMed:8002967).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76986 Genomic DNA Translation: CAA54291.1
PIRiI40579 HYBST

Genome annotation databases

KEGGiag:CAA54291

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76986 Genomic DNA Translation: CAA54291.1
PIRiI40579 HYBST

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FJ3X-ray2.00A233-548[»]
1FJOX-ray2.00A233-548[»]
1FJQX-ray1.70A233-548[»]
1FJTX-ray2.20A233-548[»]
1FJUX-ray2.00A233-548[»]
1FJVX-ray2.00A233-548[»]
1FJWX-ray1.90A233-548[»]
1GXWX-ray2.18A233-548[»]
1HYTX-ray1.70A233-548[»]
1KEIX-ray1.60A233-548[»]
1KJOX-ray1.60A233-548[»]
1KJPX-ray1.60A233-548[»]
1KKKX-ray1.60A233-548[»]
1KL6X-ray1.80A233-548[»]
1KR6X-ray1.80A233-548[»]
1KROX-ray1.70A233-548[»]
1KS7X-ray1.70A233-548[»]
1KTOX-ray1.90A233-548[»]
1L3FX-ray2.30E233-548[»]
1LNAX-ray1.90E233-548[»]
1LNBX-ray1.80E233-548[»]
1LNCX-ray1.80E233-548[»]
1LNDX-ray1.70E233-548[»]
1LNEX-ray1.70E233-548[»]
1LNFX-ray1.70E233-548[»]
1OS0X-ray2.10A233-548[»]
1PE5X-ray1.70A233-548[»]
1PE7X-ray1.82A233-548[»]
1PE8X-ray1.80A233-548[»]
1QF0X-ray2.20A233-548[»]
1QF1X-ray2.00A233-548[»]
1QF2X-ray2.06A233-548[»]
1THLX-ray1.70A233-548[»]
1TLIX-ray2.05A233-548[»]
1TLPX-ray2.30E233-548[»]
1TLXX-ray2.10A233-548[»]
1TMNX-ray1.90E233-548[»]
1TRLNMR-A/B487-548[»]
1Y3GX-ray2.10E233-548[»]
1Z9GX-ray1.70E233-548[»]
1ZDPX-ray1.70E233-548[»]
2A7GX-ray1.85E233-548[»]
2G4ZX-ray1.98A233-548[»]
2TLIX-ray1.95A233-548[»]
2TLXX-ray1.65A233-548[»]
2TMNX-ray1.60E233-548[»]
2WHZX-ray1.75A233-548[»]
2WI0X-ray1.95A233-548[»]
3DNZX-ray1.20A233-548[»]
3DO0X-ray1.36A233-548[»]
3DO1X-ray1.33A233-548[»]
3DO2X-ray1.22A233-548[»]
3EIMX-ray1.88A233-548[»]
3F28X-ray1.68A233-548[»]
3F2PX-ray1.95A233-548[»]
3FB0X-ray1.60A233-548[»]
3FBOX-ray1.92A233-548[»]
3FCQX-ray1.75A233-548[»]
3FGDX-ray1.33A233-548[»]
3FLFX-ray1.97A233-548[»]
3FORX-ray1.93A233-548[»]
3FV4X-ray1.56A233-548[»]
3FVPX-ray1.41A233-548[»]
3FXPX-ray2.05A233-548[»]
3FXSX-ray1.55A233-548[»]
3LS7X-ray1.98A233-548[»]
3MS3X-ray1.54A233-548[»]
3MSAX-ray1.66A233-548[»]
3MSFX-ray2.09A233-548[»]
3MSNX-ray1.97A233-548[»]
3N21X-ray1.87A233-548[»]
3NN7X-ray2.05A233-548[»]
3P7PX-ray2.20E233-548[»]
3P7QX-ray2.20E233-548[»]
3P7RX-ray2.20E233-548[»]
3P7SX-ray2.20E233-548[»]
3P7TX-ray2.20E233-548[»]
3P7UX-ray2.20E233-548[»]
3P7VX-ray2.20E233-548[»]
3P7WX-ray2.20E233-548[»]
3QGOX-ray1.45A233-548[»]
3QH1X-ray1.55A233-548[»]
3QH5X-ray1.50A233-548[»]
3SSBX-ray1.80A/B233-548[»]
3T2HX-ray1.95E233-548[»]
3T2IX-ray2.10E233-548[»]
3T2JX-ray2.00E233-548[»]
3T73X-ray1.60A233-548[»]
3T74X-ray1.28A233-548[»]
3T87X-ray1.28A233-548[»]
3T8CX-ray1.66A233-548[»]
3T8DX-ray1.41A233-548[»]
3T8FX-ray1.44A233-548[»]
3T8GX-ray1.50A233-548[»]
3T8HX-ray1.45A233-548[»]
3TLIX-ray1.95A233-548[»]
3TMNX-ray1.70E233-548[»]
3ZI6X-ray2.00A233-548[»]
4D91X-ray1.90A233-548[»]
4D9WX-ray1.38A233-548[»]
4H57X-ray1.56A233-548[»]
4MTWX-ray1.32E233-548[»]
4MWPX-ray1.23E233-548[»]
4MXJX-ray1.35E233-548[»]
4MZNX-ray1.17E233-548[»]
4N4EX-ray1.13E233-548[»]
4N5PX-ray1.25E233-548[»]
4N66X-ray1.44E233-548[»]
4OI5X-ray1.30E233-548[»]
4OW3X-ray2.10A233-548[»]
4TLIX-ray1.95A233-548[»]
4TLNX-ray2.30A233-548[»]
4TMNX-ray1.70E233-548[»]
4TNLX-ray1.80A233-548[»]
5A3YX-ray1.27A1-548[»]
5DPEX-ray1.34E233-548[»]
5DPFX-ray1.47E233-548[»]
5FSJX-ray1.20A233-548[»]
5FSPX-ray1.70A233-548[»]
5FSSX-ray1.50A233-548[»]
5FXNX-ray1.45A234-548[»]
5JS3X-ray1.16E233-548[»]
5JSSX-ray1.19E233-548[»]
5JT9X-ray1.26E233-548[»]
5JVIX-ray1.12E233-548[»]
5JXNX-ray1.38E233-548[»]
5K7Telectron microscopy2.50A233-548[»]
5L3UX-ray1.23E233-548[»]
5L41X-ray1.25E233-548[»]
5L8PX-ray1.29E233-548[»]
5LIFX-ray1.31E233-548[»]
5LVDX-ray1.25E233-548[»]
5LWDX-ray1.23E233-548[»]
5M5FX-ray1.33E233-548[»]
5M69X-ray1.44E233-548[»]
5M9WX-ray1.21A233-548[»]
5MA7X-ray1.30E233-548[»]
5MNRX-ray1.25E233-548[»]
5N2TX-ray1.38E233-548[»]
5N2XX-ray1.21E233-548[»]
5N2ZX-ray1.37E233-548[»]
5N31X-ray1.37E233-548[»]
5N34X-ray1.22E233-548[»]
5N3VX-ray1.12E233-548[»]
5N3YX-ray1.34E233-548[»]
5O8NX-ray1.90A233-548[»]
5ONRX-ray1.39A233-548[»]
5T9IX-ray2.09A233-548[»]
5T9KX-ray2.10A233-548[»]
5T9QX-ray2.10A233-548[»]
5TACX-ray2.04A233-548[»]
5TADX-ray2.09A233-548[»]
5TAEX-ray2.30A233-548[»]
5TAIX-ray2.30A233-548[»]
5TAJX-ray2.03A233-548[»]
5TAKX-ray2.00A233-548[»]
5TLIX-ray2.10A233-548[»]
5TLNX-ray2.30A233-548[»]
5TMNX-ray1.60E233-548[»]
5UN3X-ray1.60A233-548[»]
5UU7X-ray1.60A233-548[»]
5UU8X-ray2.50A233-548[»]
5UU9X-ray1.60A233-548[»]
5UUAX-ray1.60A233-548[»]
5UUBX-ray1.60A233-548[»]
5UUCX-ray1.60A233-548[»]
5UUDX-ray1.60A233-548[»]
5UUEX-ray1.60A233-548[»]
6D5OX-ray2.00A233-548[»]
6D5PX-ray3.00A233-548[»]
6D5QX-ray2.00A233-548[»]
6D5RX-ray2.00A233-548[»]
6D5SX-ray2.00A233-548[»]
6D5TX-ray2.00A233-548[»]
6D5UX-ray2.00A233-548[»]
6FJ2X-ray1.43A233-548[»]
6TLIX-ray2.10A233-548[»]
6TMNX-ray1.60E233-548[»]
7TLIX-ray1.95A233-548[»]
7TLNX-ray2.30A233-548[»]
8TLIX-ray2.20A233-548[»]
8TLNX-ray1.60E233-548[»]
ProteinModelPortaliP00800
SMRiP00800
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP00800
ChEMBLiCHEMBL3392
DrugBankiDB07103 2-(4-METHYLPHENOXY)ETHYLPHOSPHINATE
DB07989 2-(ACETYL-HYDROXY-AMINO)-4-METHYL-PENTANOIC ACID METHYL ESTER
DB01935 3-{[(1r)-1-Benzyl-2-Sulfanylethyl]Amino}-3-Oxopropanoic Acid
DB07673 DEAMINO-METHYL-PHENYLALANINE
DB02000 Gamma-Phenyl-Butyric Acid
DB07434 HONH-BENZYLMALONYL-L-ALANYLGLYCINE-P-NITROANILIDE
DB07506 L-BENZYLSUCCINIC ACID
DB02669 RB106

Protein family/group databases

MEROPSiM04.001

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA54291

Enzyme and pathway databases

BRENDAi3.4.24.27 708
SABIO-RKiP00800

Miscellaneous databases

EvolutionaryTraceiP00800

Family and domain databases

CDDicd09597 M4_neutral_protease, 1 hit
Gene3Di1.10.390.10, 1 hit
InterProiView protein in InterPro
IPR011096 FTP_domain
IPR025711 PepSY
IPR023612 Peptidase_M4
IPR027268 Peptidase_M4/M1_CTD_sf
IPR001570 Peptidase_M4_C_domain
IPR013856 Peptidase_M4_domain
PfamiView protein in Pfam
PF07504 FTP, 1 hit
PF03413 PepSY, 1 hit
PF01447 Peptidase_M4, 1 hit
PF02868 Peptidase_M4_C, 1 hit
PRINTSiPR00730 THERMOLYSIN
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHER_BACTH
AccessioniPrimary (citable) accession number: P00800
Secondary accession number(s): Q45779
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 18, 2006
Last modified: November 7, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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