Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chymosin

Gene

CYM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chymosin is synthesized in the mucosa of the abomasum (fourth stomach) of young (unweaned) ruminants. The enzyme hydrolyzes casein to paracasein.

Catalytic activityi

Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92PROSITE-ProRule annotation2 Publications1
Active sitei274PROSITE-ProRule annotation2 Publications1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processDigestion

Enzyme and pathway databases

BRENDAi3.4.23.4 908
SABIO-RKiP00794

Protein family/group databases

MEROPSiA01.006

Names & Taxonomyi

Protein namesi
Recommended name:
Chymosin (EC:3.4.23.4)
Alternative name(s):
Preprorennin
Gene namesi
Name:CYM
Synonyms:CPC
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi308C → D: Propeptide is not cleaved. 1 Publication1
Mutagenesisi341C → S: Propeptide is not cleaved. 1 Publication1

Protein family/group databases

Allergomei3882 Bos d Chymosin

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 163 PublicationsAdd BLAST16
PropeptideiPRO_000002598817 – 58Activation peptide1 PublicationAdd BLAST42
ChainiPRO_000002598959 – 381ChymosinAdd BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi105 ↔ 1101 Publication
Disulfide bondi265 ↔ 2691 Publication
Disulfide bondi308 ↔ 3411 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP00794
PRIDEiP00794

Expressioni

Gene expression databases

BgeeiENSBTAG00000010565 Expressed in 1 organ(s), highest expression level in lung
ExpressionAtlasiP00794 differential

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013970

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00794
SMRiP00794
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00794

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 378Peptidase A1PROSITE-ProRule annotationAdd BLAST305

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
InParanoidiP00794
KOiK01378
OMAiYFGKIYI
OrthoDBiEOG091G0JP7
TreeFamiTF314990

Family and domain databases

CDDicd05478 pepsin_A, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR034162 Pepsin_A
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00794-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRCLVVLLAV FALSQGAEIT RIPLYKGKSL RKALKEHGLL EDFLQKQQYG
60 70 80 90 100
ISSKYSGFGE VASVPLTNYL DSQYFGKIYL GTPPQEFTVL FDTGSSDFWV
110 120 130 140 150
PSIYCKSNAC KNHQRFDPRK SSTFQNLGKP LSIHYGTGSM QGILGYDTVT
160 170 180 190 200
VSNIVDIQQT VGLSTQEPGD VFTYAEFDGI LGMAYPSLAS EYSIPVFDNM
210 220 230 240 250
MNRHLVAQDL FSVYMDRNGQ ESMLTLGAID PSYYTGSLHW VPVTVQQYWQ
260 270 280 290 300
FTVDSVTISG VVVACEGGCQ AILDTGTSKL VGPSSDILNI QQAIGATQNQ
310 320 330 340 350
YGEFDIDCDN LSYMPTVVFE INGKMYPLTP SAYTSQDQGF CTSGFQSENH
360 370 380
SQKWILGDVF IREYYSVFDR ANNLVGLAKA I
Length:381
Mass (Da):42,180
Last modified:February 1, 2005 - v3
Checksum:iA4425013B4E2E56A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17A → T in AAA30446 (PubMed:3091454).Curated1
Sequence conflicti109A → G in AAA30446 (PubMed:3091454).Curated1
Sequence conflicti155V → L in AAA30447 (PubMed:6183168).Curated1
Sequence conflicti174Y → T AA sequence (PubMed:329280).Curated1
Sequence conflicti218N → D AA sequence (PubMed:329280).Curated1
Sequence conflicti218N → D AA sequence (PubMed:381305).Curated1
Sequence conflicti230D → N in AAA30448 (PubMed:6283469).Curated1
Sequence conflicti232S → C in AAA30447 (PubMed:6183168).Curated1
Sequence conflicti252T → Y AA sequence (PubMed:381305).Curated1
Sequence conflicti325M → I (PubMed:6804449).Curated1
Sequence conflicti335S → G (PubMed:6804449).Curated1
Sequence conflicti343S → T (PubMed:6804449).Curated1
Sequence conflicti380A → T (PubMed:6804449).Curated1

Polymorphismi

Forms A and B are probably allelic variants. Form B is the predominant form and differs only in one position.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti302G → D in chymosin A. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00002 mRNA Translation: AAA30447.1
J00003 mRNA Translation: AAA30448.1
M14077
, M14069, M14070, M14071, M14072, M14073, M14074, M14075 Genomic DNA Translation: AAA30446.1
EF541122 mRNA Translation: ABU41411.1
PIRiA25631 CMBO
RefSeqiNP_851337.1, NM_180994.2
UniGeneiBt.394

Genome annotation databases

EnsembliENSBTAT00000013970; ENSBTAP00000013970; ENSBTAG00000010565
GeneIDi529879
KEGGibta:529879

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00002 mRNA Translation: AAA30447.1
J00003 mRNA Translation: AAA30448.1
M14077
, M14069, M14070, M14071, M14072, M14073, M14074, M14075 Genomic DNA Translation: AAA30446.1
EF541122 mRNA Translation: ABU41411.1
PIRiA25631 CMBO
RefSeqiNP_851337.1, NM_180994.2
UniGeneiBt.394

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CMSX-ray2.30A59-381[»]
1CZIX-ray2.30E59-381[»]
3CMSX-ray2.00A59-381[»]
4AA8X-ray1.80A59-381[»]
4AUCX-ray1.60A59-381[»]
4CMSX-ray2.20A59-381[»]
ProteinModelPortaliP00794
SMRiP00794
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013970

Protein family/group databases

Allergomei3882 Bos d Chymosin
MEROPSiA01.006

Proteomic databases

PaxDbiP00794
PRIDEiP00794

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013970; ENSBTAP00000013970; ENSBTAG00000010565
GeneIDi529879
KEGGibta:529879

Organism-specific databases

CTDi229697

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
GeneTreeiENSGT00760000118929
HOGENOMiHOG000197681
HOVERGENiHBG000482
InParanoidiP00794
KOiK01378
OMAiYFGKIYI
OrthoDBiEOG091G0JP7
TreeFamiTF314990

Enzyme and pathway databases

BRENDAi3.4.23.4 908
SABIO-RKiP00794

Miscellaneous databases

EvolutionaryTraceiP00794

Gene expression databases

BgeeiENSBTAG00000010565 Expressed in 1 organ(s), highest expression level in lung
ExpressionAtlasiP00794 differential

Family and domain databases

CDDicd05478 pepsin_A, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR034162 Pepsin_A
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHYM_BOVIN
AccessioniPrimary (citable) accession number: P00794
Secondary accession number(s): A8RRP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 2005
Last modified: November 7, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again