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Protein

Actinidain

Gene
N/A
Organism
Actinidia chinensis (Kiwi) (Yangtao)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cysteine protease responsible for the cleavage of kiwellin into kissper and KiTH.1 Publication

Catalytic activityi

Specificity close to that of papain.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1511
Active sitei2881
Active sitei3081

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.14 120

Protein family/group databases

MEROPSiI29.003

Names & Taxonomyi

Protein namesi
Recommended name:
Actinidain (EC:3.4.22.14)
Short name:
Actinidin
Alternative name(s):
Allergen: Act c 1
OrganismiActinidia chinensis (Kiwi) (Yangtao)
Taxonomic identifieri3625 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesActinidiaceaeActinidia

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei1 Act d 1
3052 Act d 1.0101

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002639825 – 126Activation peptide2 PublicationsAdd BLAST102
ChainiPRO_0000026399127 – 380ActinidainAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi148 ↔ 191
Disulfide bondi182 ↔ 224
Disulfide bondi282 ↔ 332

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP00785

Expressioni

Tissue specificityi

Fruit.1 Publication

Developmental stagei

Levels of mRNA accumulate during early fruit development.1 Publication

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00785
SMRiP00785
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00785

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00785-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLPKSFVSM SLLFFSTLLI LSLAFNAKNL TQRTNDEVKA MYESWLIKYG
60 70 80 90 100
KSYNSLGEWE RRFEIFKETL RFIDEHNADT NRSYKVGLNQ FADLTDEEFR
110 120 130 140 150
STYLRFTSGS NKTKVSNRYE PRVGQVLPSY VDWRSAGAVV DIKSQGECGG
160 170 180 190 200
CWAFSAIATV EGINKIVTGV LISLSEQELI DCGRTQNTRG CNGGYITDGF
210 220 230 240 250
QFIINNGGIN TEENYPYTAQ DGECNVDLQN EKYVTIDTYE NVPYNNEWAL
260 270 280 290 300
QTAVTYQPVS VALDAAGDAF KQYSSGIFTG PCGTAVDHAV TIVGYGTEGG
310 320 330 340 350
IDYWIVKNSW DTTWGEEGYM RILRNVGGAG TCGIATMPSY PVKYNNQNHP
360 370 380
KPYSSLINPP AFSMSKDGPV GVDDGQRYSA
Length:380
Mass (Da):42,172
Last modified:June 10, 2008 - v4
Checksum:i26BFDC612D53A50B
GO

Sequence cautioni

The sequence CAA31435 differs from that shown. Reason: Frameshift at position 371.Curated
The sequence CAA31529 differs from that shown. Reason: Frameshift at position 371.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96D → G in CAA31435 (Ref. 2) Curated1
Sequence conflicti105R → G in CAA31435 (Ref. 2) Curated1
Sequence conflicti108S → G in CAA31435 (Ref. 2) Curated1
Sequence conflicti124G → S in CAA31435 (Ref. 2) Curated1
Sequence conflicti167 – 170VTGV → TSGS AA sequence (PubMed:687380).Curated4
Sequence conflicti181D → G in CAA31435 (Ref. 2) Curated1
Sequence conflicti184R → G in CAA31435 (Ref. 2) Curated1
Sequence conflicti192N → D AA sequence (PubMed:687380).Curated1
Sequence conflicti206N → D AA sequence (PubMed:687380).Curated1
Sequence conflicti212E → G in CAA31435 (Ref. 2) Curated1
Sequence conflicti223E → D AA sequence (PubMed:687380).Curated1
Sequence conflicti225N → D AA sequence (PubMed:687380).Curated1
Sequence conflicti226V → L in CAA31435 (Ref. 2) Curated1
Sequence conflicti227D → A AA sequence (PubMed:687380).Curated1
Sequence conflicti230 – 231NE → DQ AA sequence (PubMed:687380).Curated2
Sequence conflicti240E → G in CAA31435 (Ref. 2) Curated1
Sequence conflicti272Q → H in CAA31435 (Ref. 2) Curated1
Sequence conflicti272Q → H in CAA31529 (Ref. 2) Curated1
Sequence conflicti274S → A AA sequence (PubMed:687380).Curated1
Sequence conflicti286V → I in CAA31435 (Ref. 2) Curated1
Sequence conflicti286V → I in CAA31529 (Ref. 2) Curated1
Sequence conflicti290 – 291VT → IV AA sequence (PubMed:687380).Curated2
Sequence conflicti301I → V AA sequence (PubMed:687380).Curated1
Sequence conflicti307K → E in CAA31435 (Ref. 2) Curated1
Sequence conflicti349H → Y in CAA31435 (Ref. 2) Curated1
Sequence conflicti349H → Y in CAA31529 (Ref. 2) Curated1
Sequence conflicti360P → S in CAA31435 (Ref. 2) Curated1
Sequence conflicti360P → S in CAA31529 (Ref. 2) Curated1
Sequence conflicti373D → E in CAA31435 (Ref. 2) Curated1
Sequence conflicti373D → E in CAA31529 (Ref. 2) Curated1
Sequence conflicti374D → H in CAA31529 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF343446 mRNA Translation: AAK06862.1
X13013 mRNA Translation: CAA31435.1 Frameshift.
X13139 mRNA Translation: CAA31529.1 Frameshift.

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF343446 mRNA Translation: AAK06862.1
X13013 mRNA Translation: CAA31435.1 Frameshift.
X13139 mRNA Translation: CAA31529.1 Frameshift.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AECX-ray1.86A127-344[»]
1F3Qmodel-A127-344[»]
1FOGmodel-A127-344[»]
1FTVmodel-A127-344[»]
2ACTX-ray1.70A127-346[»]
ProteinModelPortaliP00785
SMRiP00785
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei1 Act d 1
3052 Act d 1.0101
MEROPSiI29.003

Proteomic databases

PRIDEiP00785

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.22.14 120

Miscellaneous databases

EvolutionaryTraceiP00785

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACTN_ACTCH
AccessioniPrimary (citable) accession number: P00785
Secondary accession number(s): Q9AXD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 10, 2008
Last modified: September 12, 2018
This is version 113 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Allergens
    Nomenclature of allergens and list of entries
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