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Protein

Papain

Gene
N/A
Organism
Carica papaya (Papaya)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1'.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1581 Publication1
Active sitei2921
Active sitei3081 Publication1

GO - Molecular functioni

  • cysteine-type peptidase activity Source: UniProtKB-KW
  • serpin family protein binding Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.2 1191
SABIO-RKiP00784

Protein family/group databases

MEROPSiI29.003

Names & Taxonomyi

Protein namesi
Recommended name:
Papain (EC:3.4.22.2)
Alternative name(s):
Papaya proteinase I
Short name:
PPI
Allergen: Car p 1
OrganismiCarica papaya (Papaya)
Taxonomic identifieri3649 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesCaricaceaeCarica

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei709 Cari p Papain

Chemistry databases

ChEMBLiCHEMBL4779

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002640619 – 133Activation peptide1 PublicationAdd BLAST115
ChainiPRO_0000026407134 – 345PapainAdd BLAST212

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi155 ↔ 1961 Publication
Disulfide bondi189 ↔ 2281 Publication
Disulfide bondi286 ↔ 3331 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

MINTiP00784

Chemistry databases

BindingDBiP00784

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00784
SMRiP00784
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00784

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00784-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAMIPSISKL LFVAICLFVY MGLSFGDFSI VGYSQNDLTS TERLIQLFES
60 70 80 90 100
WMLKHNKIYK NIDEKIYRFE IFKDNLKYID ETNKKNNSYW LGLNVFADMS
110 120 130 140 150
NDEFKEKYTG SIAGNYTTTE LSYEEVLNDG DVNIPEYVDW RQKGAVTPVK
160 170 180 190 200
NQGSCGSCWA FSAVVTIEGI IKIRTGNLNE YSEQELLDCD RRSYGCNGGY
210 220 230 240 250
PWSALQLVAQ YGIHYRNTYP YEGVQRYCRS REKGPYAAKT DGVRQVQPYN
260 270 280 290 300
EGALLYSIAN QPVSVVLEAA GKDFQLYRGG IFVGPCGNKV DHAVAAVGYG
310 320 330 340
PNYILIKNSW GTGWGENGYI RIKRGTGNSY GVCGLYTSSF YPVKN
Length:345
Mass (Da):38,922
Last modified:January 1, 1988 - v1
Checksum:i82D9FB35EDCA12EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180E → Q AA sequence (PubMed:5470818).Curated1
Sequence conflicti219 – 220YP → PY AA sequence (PubMed:5470818).Curated2
Sequence conflicti251E → Q AA sequence (PubMed:5470818).Curated1
Sequence conflicti268E → Q AA sequence (PubMed:5470818).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15203 mRNA Translation: AAB02650.1
PIRiA26466 PPPA
UniGeneiCpp.22975

Similar proteinsi

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15203 mRNA Translation: AAB02650.1
PIRiA26466 PPPA
UniGeneiCpp.22975

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BP4X-ray2.20A134-345[»]
1BQIX-ray2.50A134-345[»]
1CVZX-ray1.70A134-345[»]
1EFFmodel-A134-345[»]
1KHPX-ray2.00A134-345[»]
1KHQX-ray1.60A134-345[»]
1PADX-ray2.80A134-345[»]
1PE6X-ray2.10A134-345[»]
1PIPX-ray1.70A134-345[»]
1POPX-ray2.10A134-345[»]
1PPDX-ray2.00A134-345[»]
1PPNX-ray1.60A134-345[»]
1PPPX-ray1.90A134-345[»]
1STFX-ray2.37E134-345[»]
2CIOX-ray1.50A134-345[»]
2PADX-ray2.80A134-345[»]
3E1ZX-ray1.86B134-345[»]
3IMAX-ray2.03A/C134-345[»]
3LFYX-ray2.60A/C134-345[»]
3TNXX-ray2.62A/C27-345[»]
3USVX-ray3.80A/C27-345[»]
4PADX-ray2.80A134-345[»]
4QRGX-ray2.50A/B27-345[»]
4QRVX-ray1.98A/B27-345[»]
4QRXX-ray3.14A/C27-345[»]
5PADX-ray2.80A134-345[»]
6H8TX-ray2.10A/J134-345[»]
6PADX-ray2.80A134-345[»]
9PAPX-ray1.65A134-345[»]
ProteinModelPortaliP00784
SMRiP00784
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiP00784

Chemistry databases

BindingDBiP00784
ChEMBLiCHEMBL4779

Protein family/group databases

Allergomei709 Cari p Papain
MEROPSiI29.003

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.22.2 1191
SABIO-RKiP00784

Miscellaneous databases

EvolutionaryTraceiP00784

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPAPA1_CARPA
AccessioniPrimary (citable) accession number: P00784
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Allergens
    Nomenclature of allergens and list of entries
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