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Protein

Subtilisin BPN'

Gene

apr

Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.1 Publication

Miscellaneous

Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Activity regulationi

Completely inhibited by phenylmethylsulfony fluoride (PMSF) and partially inhibited by benzamidine hydrochloride, leupeptin, and pepstatin A.1 Publication

pH dependencei

Optimum pH is 9.0.1 Publication

Temperature dependencei

Optimum temperature is 48 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109Calcium 11
Active sitei139Charge relay system1 Publication1
Metal bindingi148Calcium 11
Active sitei171Charge relay system1 Publication1
Metal bindingi182Calcium 1; via carbonyl oxygen1
Metal bindingi184Calcium 11
Metal bindingi186Calcium 1; via carbonyl oxygen1
Metal bindingi188Calcium 1; via carbonyl oxygen1
Metal bindingi276Calcium 2; via carbonyl oxygen1
Metal bindingi278Calcium 2; via carbonyl oxygen1
Metal bindingi281Calcium 2; via carbonyl oxygen1
Active sitei328Charge relay system1 Publication1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processSporulation
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.62 630
SABIO-RKiP00782

Protein family/group databases

MEROPSiI09.001

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin BPN' (EC:3.4.21.62)
Alternative name(s):
Alkaline protease
Subtilisin DFE
Subtilisin Novo
Gene namesi
Name:apr
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

Used as a detergent protease. Sold under the name Alcalase by Novozymes.

Chemistry databases

DrugBankiDB03297 Benzylsulfinic Acid
DB04491 Diisopropylphosphono Group
DB02442 Dioxyselenocysteine
DB02325 Isopropyl Alcohol
DB01805 Monoisopropylphosphorylserine
DB01915 S-Hydroxycysteine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30By similarityAdd BLAST30
PropeptideiPRO_000002717731 – 1072 PublicationsAdd BLAST77
ChainiPRO_0000027178108 – 382Subtilisin BPN'Add BLAST275

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiP00782

Miscellaneous databases

PMAP-CutDBiP00782

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Ica-2Q400598EBI-1033955,EBI-1040468From Hordeum vulgare.

Protein-protein interaction databases

IntActiP00782, 2 interactors
MINTiP00782

Structurei

Secondary structure

1382
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00782
SMRiP00782
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00782

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 380Peptidase S8Add BLAST247

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1404 LUCA

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00782-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRGKKVWISL LFALALIFTM AFGSTSSAQA AGKSNGEKKY IVGFKQTMST
60 70 80 90 100
MSAAKKKDVI SEKGGKVQKQ FKYVDAASAT LNEKAVKELK KDPSVAYVEE
110 120 130 140 150
DHVAHAYAQS VPYGVSQIKA PALHSQGYTG SNVKVAVIDS GIDSSHPDLK
160 170 180 190 200
VAGGASMVPS ETNPFQDNNS HGTHVAGTVA ALNNSIGVLG VAPSASLYAV
210 220 230 240 250
KVLGADGSGQ YSWIINGIEW AIANNMDVIN MSLGGPSGSA ALKAAVDKAV
260 270 280 290 300
ASGVVVVAAA GNEGTSGSSS TVGYPGKYPS VIAVGAVDSS NQRASFSSVG
310 320 330 340 350
PELDVMAPGV SIQSTLPGNK YGAYNGTSMA SPHVAGAAAL ILSKHPNWTN
360 370 380
TQVRSSLENT TTKLGDSFYY GKGLINVQAA AQ
Length:382
Mass (Da):39,181
Last modified:July 21, 1986 - v1
Checksum:iED987DAFA37B8335
GO

Sequence cautioni

The sequence CAA24990 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti128Y → F in strain: DC-4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02496 Genomic DNA Translation: AAB05345.1
X00165 Genomic DNA Translation: CAA24990.1 Different initiation.
PIRiB25415 SUBSN
RefSeqiWP_013351733.1, NZ_MOEA01000001.1

Genome annotation databases

GeneIDi9780852

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02496 Genomic DNA Translation: AAB05345.1
X00165 Genomic DNA Translation: CAA24990.1 Different initiation.
PIRiB25415 SUBSN
RefSeqiWP_013351733.1, NZ_MOEA01000001.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A2QX-ray1.80A108-382[»]
1AK9X-ray1.80A108-382[»]
1AQNX-ray1.80A108-382[»]
1AU9X-ray1.80A108-382[»]
1DUIX-ray2.00A108-382[»]
1GNSX-ray1.80A112-382[»]
1GNVX-ray1.90A108-382[»]
1LW6X-ray1.50E108-382[»]
1S01X-ray1.70A108-382[»]
1S02X-ray1.90A108-382[»]
1SBHX-ray1.80A108-382[»]
1SBIX-ray2.20A108-382[»]
1SBNX-ray2.10E108-382[»]
1SBTX-ray2.50A108-382[»]
1SIBX-ray2.40E108-382[»]
1SPBX-ray2.00P37-107[»]
S108-382[»]
1ST2X-ray2.00A108-382[»]
1SUAX-ray2.10A108-382[»]
1SUBX-ray1.75A108-382[»]
1SUCX-ray1.80A108-382[»]
1SUDX-ray1.90A108-382[»]
1SUEX-ray1.80A108-382[»]
1SUPX-ray1.60A108-382[»]
1TM1X-ray1.70E108-382[»]
1TM3X-ray1.57E108-382[»]
1TM4X-ray1.70E108-382[»]
1TM5X-ray1.45E108-382[»]
1TM7X-ray1.59E108-382[»]
1TMGX-ray1.67E108-382[»]
1TO1X-ray1.68E108-382[»]
1TO2X-ray1.30E108-382[»]
1UBNX-ray2.40A108-382[»]
1V5IX-ray1.50A108-382[»]
1Y1KX-ray1.56E108-382[»]
1Y33X-ray1.80E108-382[»]
1Y34X-ray1.55E108-382[»]
1Y3BX-ray1.80E108-382[»]
1Y3CX-ray1.69E108-382[»]
1Y3DX-ray1.80E108-382[»]
1Y3FX-ray1.72E108-382[»]
1Y48X-ray1.84E108-382[»]
1Y4AX-ray1.60E108-382[»]
1Y4DX-ray2.00E108-382[»]
1YJAX-ray1.80A108-382[»]
1YJBX-ray1.80A108-382[»]
1YJCX-ray1.80A108-382[»]
2SBTX-ray2.80A108-382[»]
2SICX-ray1.80E108-382[»]
2SNIX-ray2.10E108-382[»]
2ST1X-ray1.80A108-382[»]
3BGOX-ray1.80P32-111[»]
S108-382[»]
3CNQX-ray1.71P32-111[»]
S108-382[»]
3CO0X-ray1.93P32-111[»]
S108-382[»]
3F49X-ray1.70S108-382[»]
3SICX-ray1.80E108-382[»]
5OX2X-ray2.24A108-382[»]
5SICX-ray2.20E108-382[»]
ProteinModelPortaliP00782
SMRiP00782
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00782, 2 interactors
MINTiP00782

Chemistry databases

DrugBankiDB03297 Benzylsulfinic Acid
DB04491 Diisopropylphosphono Group
DB02442 Dioxyselenocysteine
DB02325 Isopropyl Alcohol
DB01805 Monoisopropylphosphorylserine
DB01915 S-Hydroxycysteine

Protein family/group databases

MEROPSiI09.001

Proteomic databases

PRIDEiP00782

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9780852

Phylogenomic databases

eggNOGiCOG1404 LUCA

Enzyme and pathway databases

BRENDAi3.4.21.62 630
SABIO-RKiP00782

Miscellaneous databases

EvolutionaryTraceiP00782
PMAP-CutDBiP00782

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSUBT_BACAM
AccessioniPrimary (citable) accession number: P00782
Secondary accession number(s): P83945, Q44684
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 12, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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