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Protein

Subtilisin Carlsberg

Gene

apr

Organism
Bacillus licheniformis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.

Miscellaneous

Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107Calcium 11
Active sitei137By similarity1
Metal bindingi146Calcium 11
Active sitei168By similarity1
Metal bindingi179Calcium 1; via carbonyl oxygen1
Metal bindingi181Calcium 11
Metal bindingi185Calcium 1; via carbonyl oxygen1
Metal bindingi273Calcium 2; via carbonyl oxygen1
Metal bindingi275Calcium 2; via carbonyl oxygen1
Metal bindingi278Calcium 2; via carbonyl oxygen1
Active sitei325By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processSporulation
LigandCalcium, Metal-binding

Enzyme and pathway databases

SABIO-RKiP00780

Protein family/group databases

MEROPSiI09.001

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin Carlsberg (EC:3.4.21.62)
Gene namesi
Name:apr
OrganismiBacillus licheniformis
Taxonomic identifieri1402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

Used as a detergent protease. Sold under the name Alcalase by Novozymes.

Protein family/group databases

Allergomei8254 Bac li Subtilisin

Chemistry databases

ChEMBLiCHEMBL4299
DrugBankiDB02677 D-Naphthyl-1-Acetamido Boronic Acid Alanine
DB03607 D-Para-Chlorophenyl-1-Acetamidoboronic Acid Alanine
DB02560 D-Para-Chlorophenyl-1-Acteamidoboronic Acid Alanine
DB01942 Formic Acid
DB03290 L-Naphthyl-1-Acetamido Boronic Acid Alanine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29By similarityAdd BLAST29
PropeptideiPRO_000002717930 – 1051 PublicationAdd BLAST76
ChainiPRO_0000027180106 – 379Subtilisin CarlsbergAdd BLAST274

Keywords - PTMi

Zymogen

Interactioni

Protein-protein interaction databases

IntActiP00780, 2 interactors
MINTiP00780

Chemistry databases

BindingDBiP00780

Structurei

Secondary structure

1379
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00780
SMRiP00780
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00780

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 377Peptidase S8Add BLAST246

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105RX7 Bacteria
COG1404 LUCA

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00780-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMRKKSFWLG MLTAFMLVFT MAFSDSASAA QPAKNVEKDY IVGFKSGVKT
60 70 80 90 100
ASVKKDIIKE SGGKVDKQFR IINAAKAKLD KEALKEVKND PDVAYVEEDH
110 120 130 140 150
VAHALAQTVP YGIPLIKADK VQAQGFKGAN VKVAVLDTGI QASHPDLNVV
160 170 180 190 200
GGASFVAGEA YNTDGNGHGT HVAGTVAALD NTTGVLGVAP SVSLYAVKVL
210 220 230 240 250
NSSGSGTYSG IVSGIEWATT NGMDVINMSL GGPSGSTAMK QAVDNAYARG
260 270 280 290 300
VVVVAAAGNS GSSGNTNTIG YPAKYDSVIA VGAVDSNSNR ASFSSVGAEL
310 320 330 340 350
EVMAPGAGVY STYPTSTYAT LNGTSMASPH VAGAAALILS KHPNLSASQV
360 370
RNRLSSTATY LGSSFYYGKG LINVEAAAQ
Length:379
Mass (Da):38,908
Last modified:August 1, 1988 - v1
Checksum:iF19A6DC5761FB504
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207T → S AA sequence (PubMed:4967581).Curated1
Sequence conflicti233P → A AA sequence (PubMed:4967581).Curated1
Sequence conflicti262 – 265SSGN → NSGS AA sequence (PubMed:4967581).Curated4
Sequence conflicti316S → N AA sequence (PubMed:4967581).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03341 Genomic DNA Translation: CAB56500.1
PIRiA24111 SUBSCL

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03341 Genomic DNA Translation: CAB56500.1
PIRiA24111 SUBSCL

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AF4X-ray2.60A106-379[»]
1AV7X-ray2.60A106-379[»]
1AVTX-ray2.00A106-379[»]
1BE6X-ray2.15A106-379[»]
1BE8X-ray2.20A106-379[»]
1BFKX-ray2.30A106-379[»]
1BFUX-ray2.20A106-379[»]
1C3LX-ray2.16A106-378[»]
1CSEX-ray1.20E106-379[»]
1OYVX-ray2.50A/B106-379[»]
1R0RX-ray1.10E106-378[»]
1SBCX-ray2.50A106-379[»]
1SCAX-ray2.00A106-379[»]
1SCBX-ray2.30A106-379[»]
1SCDX-ray2.30A106-379[»]
1SCNX-ray1.90E106-379[»]
1SELX-ray2.00A/B106-379[»]
1VSBX-ray2.10A106-379[»]
1YU6X-ray1.55A/B106-379[»]
2SECX-ray1.80E106-379[»]
2WUVX-ray2.24A106-379[»]
2WUWX-ray2.23E106-379[»]
3UNXX-ray1.26A106-379[»]
3VSBX-ray2.60A106-379[»]
4C3UX-ray2.29A106-379[»]
4C3VX-ray2.26A106-379[»]
ProteinModelPortaliP00780
SMRiP00780
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00780, 2 interactors
MINTiP00780

Chemistry databases

BindingDBiP00780
ChEMBLiCHEMBL4299
DrugBankiDB02677 D-Naphthyl-1-Acetamido Boronic Acid Alanine
DB03607 D-Para-Chlorophenyl-1-Acetamidoboronic Acid Alanine
DB02560 D-Para-Chlorophenyl-1-Acteamidoboronic Acid Alanine
DB01942 Formic Acid
DB03290 L-Naphthyl-1-Acetamido Boronic Acid Alanine

Protein family/group databases

Allergomei8254 Bac li Subtilisin
MEROPSiI09.001

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105RX7 Bacteria
COG1404 LUCA

Enzyme and pathway databases

SABIO-RKiP00780

Miscellaneous databases

EvolutionaryTraceiP00780

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSUBT_BACLI
AccessioniPrimary (citable) accession number: P00780
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1988
Last modified: September 12, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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