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UniProtKB - P00778 (PRLA_LYSEN)
Protein
Alpha-lytic protease
Gene
alpha-LP
Organism
Lysobacter enzymogenes
Status
Functioni
Catalytic activityi
- Preferential cleavage: Ala-|-Xaa, Val-|-Xaa in bacterial cell walls, elastin and other proteins. EC:3.4.21.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 235 | Charge relay systemBy similarity | 1 | |
Active sitei | 262 | Charge relay systemBy similarity | 1 | |
Active sitei | 342 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BRENDAi | 3.4.21.12, 3118 |
Protein family/group databases
MEROPSi | S01.268 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:alpha-LP |
Organismi | Lysobacter enzymogenes |
Taxonomic identifieri | 69 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Lysobacter |
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL3085615 |
DrugBanki | DB07411, PHENYLALANINE BORONIC ACID DB04237, Tris(Hydroxyethyl)Aminomethane |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
PropeptideiPRO_0000026907 | 25 – 199 | Add BLAST | 175 | |
ChainiPRO_0000026908 | 200 – 397 | Alpha-lytic proteaseAdd BLAST | 198 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 216 ↔ 236 | |||
Disulfide bondi | 300 ↔ 310 | |||
Disulfide bondi | 336 ↔ 369 |
Keywords - PTMi
Disulfide bond, ZymogenStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P00778 |
SMRi | P00778 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00778 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S1 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
OMAi | NVRCSIG |
Family and domain databases
Gene3Di | 2.40.10.10, 2 hits 3.30.300.50, 2 hits |
InterProi | View protein in InterPro IPR037295, Alpha-lytic_protease_prodomain IPR004236, Pept_S1_alpha_lytic IPR001316, Pept_S1A_streptogrisin IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR035070, Streptogrisin_prodomain IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF02983, Pro_Al_protease, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001134, Streptogrisin, 1 hit |
PRINTSi | PR00861, ALYTICPTASE |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF54806, SSF54806, 2 hits |
PROSITEi | View protein in PROSITE PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00778-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYVSNHRSRR VARVSVSCLV AALAAMSCGA ALAADQVDPQ LKFAMQRDLG
60 70 80 90 100
IFPTQLPQYL QTEKLARTQA AAIEREFGAQ FAGSWIERNE DGSFKLVAAT
110 120 130 140 150
SGARKSSTLG GVEVRNVRYS LKQLQSAMEQ LDAGANARVK GVSKPLDGVQ
160 170 180 190 200
SWYVDPRSNA VVVKVDDGAT EAGVDFVALS GADSAQVRIE SSPGKLQTTA
210 220 230 240 250
NIVGGIEYSI NNASLCSVGF SVTRGATKGF VTAGHCGTVN ATARIGGAVV
260 270 280 290 300
GTFAARVFPG NDRAWVSLTS AQTLLPRVAN GSSFVTVRGS TEAAVGAAVC
310 320 330 340 350
RSGRTTGYQC GTITAKNVTA NYAEGAVRGL TQGNACMGRG DSGGSWITSA
360 370 380 390
GQAQGVMSGG NVQSNGNNCG IPASQRSSLF ERLQPILSQY GLSLVTG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 171 | E → D in AAA74111 (PubMed:3234766).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04052 Genomic DNA Translation: AAA25409.1 M22763 Genomic DNA Translation: AAA74111.1 |
PIRi | A31772, TRYXB4 |
Genome annotation databases
KEGGi | ag:AAA25409 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04052 Genomic DNA Translation: AAA25409.1 M22763 Genomic DNA Translation: AAA74111.1 |
PIRi | A31772, TRYXB4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BOQ | X-ray | 2.10 | A | 200-397 | [»] | |
1GBA | X-ray | 2.15 | A | 200-397 | [»] | |
1GBB | X-ray | 2.15 | A | 200-397 | [»] | |
1GBC | X-ray | 2.20 | A | 200-397 | [»] | |
1GBD | X-ray | 2.20 | A | 200-397 | [»] | |
1GBE | X-ray | 2.30 | A | 200-397 | [»] | |
1GBF | X-ray | 2.15 | A | 200-397 | [»] | |
1GBH | X-ray | 2.20 | A | 200-397 | [»] | |
1GBI | X-ray | 2.30 | A | 200-397 | [»] | |
1GBJ | X-ray | 2.00 | A | 200-397 | [»] | |
1GBK | X-ray | 2.13 | A | 200-397 | [»] | |
1GBL | X-ray | 2.15 | A | 200-397 | [»] | |
1GBM | X-ray | 2.28 | A | 200-397 | [»] | |
1P01 | X-ray | 2.00 | A | 200-397 | [»] | |
1P02 | X-ray | 2.00 | A | 200-397 | [»] | |
1P03 | X-ray | 2.15 | A | 200-397 | [»] | |
1P04 | X-ray | 2.55 | A | 200-397 | [»] | |
1P05 | X-ray | 2.10 | A | 200-397 | [»] | |
1P06 | X-ray | 2.34 | A | 200-397 | [»] | |
1P09 | X-ray | 2.20 | A | 200-397 | [»] | |
1P10 | X-ray | 2.25 | A | 200-397 | [»] | |
1P11 | X-ray | 1.93 | E | 200-397 | [»] | |
1P12 | X-ray | 1.90 | E | 200-397 | [»] | |
1QQ4 | X-ray | 1.20 | A | 200-397 | [»] | |
1QRW | X-ray | 1.20 | A | 200-397 | [»] | |
1QRX | X-ray | 1.60 | A | 200-397 | [»] | |
1SSX | X-ray | 0.83 | A | 200-397 | [»] | |
1TAL | X-ray | 1.50 | A | 200-397 | [»] | |
2ALP | X-ray | 1.70 | A | 200-397 | [»] | |
2H5C | X-ray | 0.82 | A | 200-397 | [»] | |
2H5D | X-ray | 0.90 | A | 200-397 | [»] | |
2LPR | X-ray | 2.25 | A | 200-397 | [»] | |
2PRO | X-ray | 3.00 | A/B/C | 34-199 | [»] | |
2ULL | X-ray | 1.50 | A | 200-397 | [»] | |
3LPR | X-ray | 2.15 | A | 200-397 | [»] | |
3M7T | X-ray | 1.55 | A | 200-397 | [»] | |
3M7U | X-ray | 1.05 | A | 200-397 | [»] | |
3PRO | X-ray | 1.80 | A/B | 200-397 | [»] | |
C/D | 34-199 | [»] | ||||
3QGJ | X-ray | 1.30 | A/C | 200-397 | [»] | |
3URC | X-ray | 1.10 | A | 200-397 | [»] | |
3URD | X-ray | 1.08 | A | 200-397 | [»] | |
3URE | X-ray | 1.49 | A/B | 200-397 | [»] | |
4PRO | X-ray | 2.40 | A/B | 200-397 | [»] | |
C/D | 34-199 | [»] | ||||
5LPR | X-ray | 2.13 | A | 200-397 | [»] | |
5WOT | NMR | - | A | 200-397 | [»] | |
6LPR | X-ray | 2.10 | A | 200-397 | [»] | |
7LPR | X-ray | 2.05 | A | 200-397 | [»] | |
8LPR | X-ray | 2.25 | A | 200-397 | [»] | |
9LPR | X-ray | 2.20 | A | 200-397 | [»] | |
BMRBi | P00778 | |||||
SMRi | P00778 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
ChEMBLi | CHEMBL3085615 |
DrugBanki | DB07411, PHENYLALANINE BORONIC ACID DB04237, Tris(Hydroxyethyl)Aminomethane |
Protein family/group databases
MEROPSi | S01.268 |
Genome annotation databases
KEGGi | ag:AAA25409 |
Phylogenomic databases
OMAi | NVRCSIG |
Enzyme and pathway databases
BRENDAi | 3.4.21.12, 3118 |
Miscellaneous databases
EvolutionaryTracei | P00778 |
Family and domain databases
Gene3Di | 2.40.10.10, 2 hits 3.30.300.50, 2 hits |
InterProi | View protein in InterPro IPR037295, Alpha-lytic_protease_prodomain IPR004236, Pept_S1_alpha_lytic IPR001316, Pept_S1A_streptogrisin IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR035070, Streptogrisin_prodomain IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF02983, Pro_Al_protease, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001134, Streptogrisin, 1 hit |
PRINTSi | PR00861, ALYTICPTASE |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF54806, SSF54806, 2 hits |
PROSITEi | View protein in PROSITE PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PRLA_LYSEN | |
Accessioni | P00778Primary (citable) accession number: P00778 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | February 1, 1996 | |
Last modified: | September 29, 2021 | |
This is version 133 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families