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UniProtKB - P00763 (TRY2_RAT)
Protein
Anionic trypsin-2
Gene
Prss2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalytic activityi
- Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. EC:3.4.21.4
Cofactori
Ca2+Note: Binds 1 Ca2+ ion per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 63 | Charge relay system | 1 | |
Metal bindingi | 75 | Calcium | 1 | |
Metal bindingi | 77 | Calcium; via carbonyl oxygen | 1 | |
Metal bindingi | 80 | Calcium; via carbonyl oxygen | 1 | |
Metal bindingi | 85 | Calcium | 1 | |
Active sitei | 107 | Charge relay system | 1 | |
Sitei | 194 | Required for specificityBy similarity | 1 | |
Active sitei | 200 | Charge relay system | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- serine-type endopeptidase activity Source: UniProtKB
GO - Biological processi
- collagen catabolic process Source: UniProtKB
- digestion Source: RGD
- proteolysis Source: UniProtKB
- response to nutrient Source: RGD
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Digestion |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
SABIO-RKi | P00763 |
Protein family/group databases
MEROPSi | S01.119 |
Names & Taxonomyi
Protein namesi | Recommended name: Anionic trypsin-2 (EC:3.4.21.4)Alternative name(s): Anionic trypsin II Pretrypsinogen II Serine protease 2 |
Gene namesi | Name:Prss2 Synonyms:Try2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3418, Prss2 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB
- extracellular space Source: UniProtKB
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | Add BLAST | 15 | |
PropeptideiPRO_0000028209 | 16 – 23 | Activation peptide | 8 | |
ChainiPRO_0000028210 | 24 – 246 | Anionic trypsin-2Add BLAST | 223 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 30 ↔ 160 | |||
Disulfide bondi | 48 ↔ 64 | |||
Disulfide bondi | 132 ↔ 233 | |||
Disulfide bondi | 139 ↔ 206 | |||
Disulfide bondi | 171 ↔ 185 | |||
Disulfide bondi | 196 ↔ 220 |
Keywords - PTMi
Disulfide bond, ZymogenProteomic databases
PaxDbi | P00763 |
PRIDEi | P00763 |
PTM databases
iPTMneti | P00763 |
PhosphoSitePlusi | P00763 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000032916, Expressed in pancreas and 11 other tissues |
Genevisiblei | P00763, RN |
Interactioni
Binary interactionsi
P00763
With | #Exp. | IntAct |
---|---|---|
eco [P23827] from Escherichia coli (strain K12). | 2 | EBI-1029166,EBI-1029159 |
Protein-protein interaction databases
BioGRIDi | 247129, 1 interactor |
DIPi | DIP-6112N |
IntActi | P00763, 2 interactors |
MINTi | P00763 |
STRINGi | 10116.ENSRNOP00000018853 |
Chemistry databases
BindingDBi | P00763 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00763 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00763 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 244 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 221 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00990000203555 |
HOGENOMi | CLU_006842_7_0_1 |
InParanoidi | P00763 |
OMAi | HADCEAS |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P00763 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00763-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRALLFLALV GAAVAFPVDD DDKIVGGYTC QENSVPYQVS LNSGYHFCGG
60 70 80 90 100
SLINDQWVVS AAHCYKSRIQ VRLGEHNINV LEGNEQFVNA AKIIKHPNFD
110 120 130 140 150
RKTLNNDIML IKLSSPVKLN ARVATVALPS SCAPAGTQCL ISGWGNTLSS
160 170 180 190 200
GVNEPDLLQC LDAPLLPQAD CEASYPGKIT DNMVCVGFLE GGKDSCQGDS
210 220 230 240
GGPVVCNGEL QGIVSWGYGC ALPDNPGVYT KVCNYVDWIQ DTIAAN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 | N → D (PubMed:6094547).Curated | 1 | |
Sequence conflicti | 88 | V → I (PubMed:6094547).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01274 mRNA Translation: CAA24581.1 L00131, L00130 Genomic DNA Translation: AAA98517.1 |
PIRi | A22657, TRRT2 |
RefSeqi | NP_036861.1, NM_012729.2 |
Genome annotation databases
Ensembli | ENSRNOT00000018852; ENSRNOP00000018853; ENSRNOG00000032916 |
GeneIDi | 25052 |
KEGGi | rno:25052 |
UCSCi | RGD:3418, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01274 mRNA Translation: CAA24581.1 L00131, L00130 Genomic DNA Translation: AAA98517.1 |
PIRi | A22657, TRRT2 |
RefSeqi | NP_036861.1, NM_012729.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AMH | X-ray | 2.50 | A/B | 24-246 | [»] | |
1ANB | X-ray | 2.80 | A | 24-246 | [»] | |
1ANC | X-ray | 2.20 | A | 24-246 | [»] | |
1AND | X-ray | 2.30 | A | 24-246 | [»] | |
1ANE | X-ray | 2.20 | A | 24-246 | [»] | |
1BRA | X-ray | 2.20 | A | 24-246 | [»] | |
1BRB | X-ray | 2.10 | E | 24-246 | [»] | |
1BRC | X-ray | 2.50 | E | 24-246 | [»] | |
1CO7 | X-ray | 1.90 | E | 2-246 | [»] | |
1DPO | X-ray | 1.59 | A | 24-246 | [»] | |
1EZS | X-ray | 2.30 | C/D | 24-246 | [»] | |
1EZU | X-ray | 2.40 | C/D | 24-246 | [»] | |
1F5R | X-ray | 1.65 | A | 15-246 | [»] | |
1F7Z | X-ray | 1.55 | A | 15-246 | [»] | |
1FY8 | X-ray | 1.70 | E | 15-246 | [»] | |
1J14 | X-ray | 2.40 | A | 24-246 | [»] | |
1J15 | X-ray | 2.00 | A | 24-246 | [»] | |
1J16 | X-ray | 1.60 | A | 24-246 | [»] | |
1J17 | X-ray | 2.00 | T | 24-246 | [»] | |
1K9O | X-ray | 2.30 | E | 24-246 | [»] | |
1QL9 | X-ray | 2.30 | A | 24-246 | [»] | |
1SLU | X-ray | 1.80 | B | 24-246 | [»] | |
1SLV | X-ray | 2.30 | B | 24-246 | [»] | |
1SLW | X-ray | 2.00 | B | 24-246 | [»] | |
1SLX | X-ray | 2.20 | B | 24-246 | [»] | |
1TRM | X-ray | 2.30 | A/B | 24-246 | [»] | |
1YKT | X-ray | 1.70 | A | 24-246 | [»] | |
1YLC | X-ray | 1.70 | A | 24-246 | [»] | |
1YLD | X-ray | 1.70 | A | 24-246 | [»] | |
2TRM | X-ray | 2.80 | A | 24-246 | [»] | |
3FP6 | X-ray | 1.49 | E | 24-246 | [»] | |
3FP7 | X-ray | 1.46 | E | 24-246 | [»] | |
3FP8 | X-ray | 1.46 | E | 24-246 | [»] | |
3TGI | X-ray | 1.80 | E | 24-246 | [»] | |
3TGJ | X-ray | 2.20 | E | 15-246 | [»] | |
3TGK | X-ray | 1.70 | E | 15-246 | [»] | |
SMRi | P00763 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 247129, 1 interactor |
DIPi | DIP-6112N |
IntActi | P00763, 2 interactors |
MINTi | P00763 |
STRINGi | 10116.ENSRNOP00000018853 |
Chemistry databases
BindingDBi | P00763 |
ChEMBLi | CHEMBL4290 |
Protein family/group databases
MEROPSi | S01.119 |
PTM databases
iPTMneti | P00763 |
PhosphoSitePlusi | P00763 |
Proteomic databases
PaxDbi | P00763 |
PRIDEi | P00763 |
Genome annotation databases
Ensembli | ENSRNOT00000018852; ENSRNOP00000018853; ENSRNOG00000032916 |
GeneIDi | 25052 |
KEGGi | rno:25052 |
UCSCi | RGD:3418, rat |
Organism-specific databases
CTDi | 5645 |
RGDi | 3418, Prss2 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00990000203555 |
HOGENOMi | CLU_006842_7_0_1 |
InParanoidi | P00763 |
OMAi | HADCEAS |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P00763 |
Enzyme and pathway databases
SABIO-RKi | P00763 |
Miscellaneous databases
EvolutionaryTracei | P00763 |
PROi | PR:P00763 |
Gene expression databases
Bgeei | ENSRNOG00000032916, Expressed in pancreas and 11 other tissues |
Genevisiblei | P00763, RN |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRY2_RAT | |
Accessioni | P00763Primary (citable) accession number: P00763 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families