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Protein

Glandular kallikrein

Gene
N/A
Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Caution

Native porcine kallikrein is a monomer. Chains of the pancreatic beta-kallikrein are heterogeneous artifacts of proteolytic degradation during isolation.Curated

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48Charge relay system1
Active sitei103Charge relay system1
Active sitei198Charge relay system1

GO - Molecular functioni

GO - Biological processi

  • regulation of systemic arterial blood pressure Source: GO_Central
  • zymogen activation Source: GO_Central

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Glandular kallikrein (EC:3.4.21.35)
Alternative name(s):
Tissue kallikrein
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3243909

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000279641 – 72 Publications7
ChainiPRO_00000279658 – 246Glandular kallikreinAdd BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi14 ↔ 158
Disulfide bondi33 ↔ 49
Glycosylationi85N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi135 ↔ 204
Disulfide bondi169 ↔ 183
Disulfide bondi194 ↔ 219
Glycosylationi239N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP00752
PRIDEiP00752

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000027121

Chemistry databases

BindingDBiP00752

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00752
SMRiP00752
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00752

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 243Peptidase S1PROSITE-ProRule annotationAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 104Kallikrein (autolysis) loopAdd BLAST20

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOVERGENiHBG013304

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00752-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
APPIQSRIIG GRECEKNSHP WQVAIYHYSS FQCGGVLVNP KWVLTAAHCK
60 70 80 90 100
NDNYEVWLGR HNLFENENTA QFFGVTADFP HPGFNLSLLK XHTKADGKDY
110 120 130 140 150
SHDLMLLRLQ SPAKITDAVK VLELPTQEPE LGSTCEASGW GSIEPGPDBF
160 170 180 190 200
EFPDEIQCVQ LTLLQNTFCA BAHPBKVTES MLCAGYLPGG KDTCMGDSGG
210 220 230 240
PLICNGMWQG ITSWGHTPCG SANKPSIYTK LIFYLDWIND TITENP
Length:246
Mass (Da):27,172
Last modified:March 27, 2002 - v4
Checksum:i5991CEDE406A19A1
GO

Sequence databases

PIRiA00938 KQPG

Similar proteinsi

Cross-referencesi

Sequence databases

PIRiA00938 KQPG

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HIAX-ray2.40A/X8-87[»]
B/Y95-246[»]
2KAIX-ray2.50A8-87[»]
B95-246[»]
2PKAX-ray2.05A/X8-87[»]
B/Y95-246[»]
ProteinModelPortaliP00752
SMRiP00752
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000027121

Chemistry databases

BindingDBiP00752
ChEMBLiCHEMBL3243909

Proteomic databases

PaxDbiP00752
PRIDEiP00752

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOVERGENiHBG013304

Miscellaneous databases

EvolutionaryTraceiP00752

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKLK_PIG
AccessioniPrimary (citable) accession number: P00752
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 27, 2002
Last modified: November 7, 2018
This is version 127 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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