UniProtKB - P00750 (TPA_HUMAN)
Tissue-type plasminogen activator
PLAT
Functioni
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).
By similarityCatalytic activityi
- Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. EC:3.4.21.68
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 102 | Important for binding to LRP1 | 1 | |
Active sitei | 357 | Charge relay system1 Publication | 1 | |
Active sitei | 406 | Charge relay system1 Publication | 1 | |
Sitei | 464 | Important for single-chain activity | 1 | |
Sitei | 512 | Important for single-chain activity | 1 | |
Active sitei | 513 | Charge relay system1 Publication | 1 |
GO - Molecular functioni
- phosphoprotein binding Source: AgBase
- serine-type endopeptidase activity Source: BHF-UCL
- signaling receptor binding Source: AgBase
GO - Biological processi
- blood coagulation Source: ProtInc
- cellular protein modification process Source: ProtInc
- cellular response to dexamethasone stimulus Source: Ensembl
- cellular response to follicle-stimulating hormone stimulus Source: Ensembl
- cellular response to luteinizing hormone stimulus Source: Ensembl
- fibrinolysis Source: Reactome
- negative regulation of proteolysis Source: BHF-UCL
- plasminogen activation Source: UniProtKB
- platelet-derived growth factor receptor signaling pathway Source: GO_Central
- positive regulation of ovulation Source: Ensembl
- prevention of polyspermy Source: UniProtKB
- proteolysis Source: GO_Central
- regulation of synaptic plasticity Source: Ensembl
- response to cAMP Source: Ensembl
- response to fatty acid Source: Ensembl
- response to hypoxia Source: Ensembl
- smooth muscle cell migration Source: GO_Central
- synaptic transmission, glutamatergic Source: Ensembl
- trans-synaptic signaling by BDNF, modulating synaptic transmission Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Plasminogen activation |
Enzyme and pathway databases
BRENDAi | 3.4.21.68, 2681 |
PathwayCommonsi | P00750 |
Reactomei | R-HSA-186797, Signaling by PDGF R-HSA-75205, Dissolution of Fibrin Clot |
SignaLinki | P00750 |
SIGNORi | P00750 |
Protein family/group databases
MEROPSi | S01.232 |
Names & Taxonomyi
Protein namesi | Recommended name: Tissue-type plasminogen activatorCurated (EC:3.4.21.68)Short name: t-PA Short name: t-plasminogen activator Short name: tPA Alternative name(s): INN: Alteplase INN: Reteplase Cleaved into the following 2 chains: |
Gene namesi | Name:PLATImported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9051, PLAT |
MIMi | 173370, gene |
neXtProti | NX_P00750 |
VEuPathDBi | HostDB:ENSG00000104368 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
- extracellular space Source: GO_Central
- perisynaptic space Source: Ensembl
Other locations
- apical part of cell Source: Ensembl
- cell surface Source: BHF-UCL
- cytoplasm Source: BHF-UCL
- glutamatergic synapse Source: Ensembl
- neuronal dense core vesicle Source: Ensembl
- postsynapse Source: Ensembl
- Schaffer collateral - CA1 synapse Source: Ensembl
- serine protease inhibitor complex Source: ComplexPortal
Keywords - Cellular componenti
SecretedPathology & Biotechi
Involvement in diseasei
Pharmaceutical usei
Organism-specific databases
DisGeNETi | 5327 |
MalaCardsi | PLAT |
OpenTargetsi | ENSG00000104368 |
Orphaneti | 480528, Lethal hydranencephaly-diaphragmatic hernia syndrome 64738, NON RARE IN EUROPE: Non rare thrombophilia |
PharmGKBi | PA33381 |
Miscellaneous databases
Pharosi | P00750, Tclin |
Chemistry databases
ChEMBLi | CHEMBL1873 |
DrugBanki | DB07684, 5-(DIMETHYLAMINO)-2-NAPHTHALENESULFONIC ACID DB00513, Aminocaproic acid DB09228, Conestat alfa DB09213, Dexibuprofen DB06404, Human C1-esterase inhibitor DB01088, Iloprost |
DrugCentrali | P00750 |
GuidetoPHARMACOLOGYi | 2392 |
Genetic variation databases
BioMutai | PLAT |
DMDMi | 137119 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | 1 PublicationAdd BLAST | 22 | |
PropeptideiPRO_0000028348 | 23 – 32 | 1 Publication | 10 | |
PropeptideiPRO_0000028349 | 33 – 35 | Removed by plasmin1 Publication | 3 | |
ChainiPRO_0000028350 | 36 – 562 | Tissue-type plasminogen activatorAdd BLAST | 527 | |
ChainiPRO_0000028351 | 36 – 310 | Tissue-type plasminogen activator chain AAdd BLAST | 275 | |
ChainiPRO_0000028352 | 311 – 562 | Tissue-type plasminogen activator chain BAdd BLAST | 252 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 41 ↔ 71 | 1 Publication | ||
Disulfide bondi | 69 ↔ 78 | 1 Publication | ||
Disulfide bondi | 86 ↔ 97 | 1 Publication | ||
Disulfide bondi | 91 ↔ 108 | 1 Publication | ||
GlycosylationiCAR_000029 | 96 | O-linked (Fuc) threonine1 Publication | 1 | |
Disulfide bondi | 110 ↔ 119 | 1 Publication | ||
Disulfide bondi | 127 ↔ 208 | By similarity | ||
Disulfide bondi | 148 ↔ 190 | By similarity | ||
Glycosylationi | 152 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 179 ↔ 203 | By similarity | ||
Disulfide bondi | 215 ↔ 296 | 1 Publication | ||
GlycosylationiCAR_000030 | 219 | N-linked (GlcNAc...) asparagine; partial1 Publication | 1 | |
Disulfide bondi | 236 ↔ 278 | 1 Publication | ||
Disulfide bondi | 267 ↔ 291 | 1 Publication | ||
Disulfide bondi | 299 ↔ 430 | Interchain (between A and B chains)PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 342 ↔ 358 | By similarity | ||
Disulfide bondi | 350 ↔ 419 | By similarity | ||
Disulfide bondi | 444 ↔ 519 | By similarity | ||
Disulfide bondi | 476 ↔ 492 | By similarity | ||
GlycosylationiCAR_000031 | 483 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 509 ↔ 537 | By similarity |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 253 | Not glycosylated | 1 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
CPTACi | non-CPTAC-1164 |
EPDi | P00750 |
jPOSTi | P00750 |
MassIVEi | P00750 |
MaxQBi | P00750 |
PaxDbi | P00750 |
PeptideAtlasi | P00750 |
PRIDEi | P00750 |
ProteomicsDBi | 51281 [P00750-1] 51282 [P00750-2] 51283 [P00750-3] 51284 [P00750-4] |
PTM databases
GlyConnecti | 603, 43 N-Linked glycans (3 sites), 1 O-Linked glycan (1 site) 86, 16 N-Linked glycans (1 site) |
GlyGeni | P00750, 6 sites, 103 N-linked glycans (4 sites), 1 O-linked glycan (1 site) |
iPTMneti | P00750 |
PhosphoSitePlusi | P00750 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000104368, Expressed in stromal cell of endometrium and 228 other tissues |
ExpressionAtlasi | P00750, baseline and differential |
Genevisiblei | P00750, HS |
Organism-specific databases
HPAi | ENSG00000104368, Tissue enhanced (parathyroid gland, urinary bladder) |
Interactioni
Subunit structurei
Heterodimer of chain A and chain B held by a disulfide bond.
Forms a heterodimer with SERPINA5. Binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-fold and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binds to annexin A2, cytokeratin-8, fibronectin and laminin. Binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1); these proteins are involved in TPA clearance. Yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold. Binds LRP1B; binding is followed by internalization and degradation. In complex with SERPINE1, interacts with SORL1 (PubMed:15053742).
1 PublicationGO - Molecular functioni
- phosphoprotein binding Source: AgBase
- signaling receptor binding Source: AgBase
Protein-protein interaction databases
BioGRIDi | 111343, 50 interactors |
ComplexPortali | CPX-494, tPA-PAI-1 complex |
ELMi | P00750 |
IntActi | P00750, 14 interactors |
MINTi | P00750 |
STRINGi | 9606.ENSP00000220809 |
Chemistry databases
BindingDBi | P00750 |
Miscellaneous databases
RNActi | P00750, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P00750 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00750 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 81 | Fibronectin type-IPROSITE-ProRule annotationAdd BLAST | 43 | |
Domaini | 82 – 120 | EGF-likePROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 127 – 208 | Kringle 1PROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 215 – 296 | Kringle 2PROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 311 – 561 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 251 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 42 – 52 | Important for binding to annexin A2Add BLAST | 11 |
Domaini
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Kringle, Repeat, SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000158930 |
HOGENOMi | CLU_006842_18_4_1 |
InParanoidi | P00750 |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P00750 |
TreeFami | TF329901 |
Family and domain databases
CDDi | cd00061, FN1, 1 hit cd00108, KR, 2 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.40.20.10, 2 hits |
InterProi | View protein in InterPro IPR000742, EGF-like_dom IPR000083, Fibronectin_type1 IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR026280, Tissue_plasm_act IPR034811, tPA IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24264:SF42, PTHR24264:SF42, 3 hits |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00039, fn1, 1 hit PF00051, Kringle, 2 hits PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001145, Tissue_plasm_act, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00058, FN1, 1 hit SM00130, KR, 2 hits SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS01253, FN1_1, 1 hit PS51091, FN1_2, 1 hit PS00021, KRINGLE_1, 2 hits PS50070, KRINGLE_2, 2 hits PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDAMKRGLCC VLLLCGAVFV SPSQEIHARF RRGARSYQVI CRDEKTQMIY
60 70 80 90 100
QQHQSWLRPV LRSNRVEYCW CNSGRAQCHS VPVKSCSEPR CFNGGTCQQA
110 120 130 140 150
LYFSDFVCQC PEGFAGKCCE IDTRATCYED QGISYRGTWS TAESGAECTN
160 170 180 190 200
WNSSALAQKP YSGRRPDAIR LGLGNHNYCR NPDRDSKPWC YVFKAGKYSS
210 220 230 240 250
EFCSTPACSE GNSDCYFGNG SAYRGTHSLT ESGASCLPWN SMILIGKVYT
260 270 280 290 300
AQNPSAQALG LGKHNYCRNP DGDAKPWCHV LKNRRLTWEY CDVPSCSTCG
310 320 330 340 350
LRQYSQPQFR IKGGLFADIA SHPWQAAIFA KHRRSPGERF LCGGILISSC
360 370 380 390 400
WILSAAHCFQ ERFPPHHLTV ILGRTYRVVP GEEEQKFEVE KYIVHKEFDD
410 420 430 440 450
DTYDNDIALL QLKSDSSRCA QESSVVRTVC LPPADLQLPD WTECELSGYG
460 470 480 490 500
KHEALSPFYS ERLKEAHVRL YPSSRCTSQH LLNRTVTDNM LCAGDTRSGG
510 520 530 540 550
PQANLHDACQ GDSGGPLVCL NDGRMTLVGI ISWGLGCGQK DVPGVYTKVT
560
NYLDWIRDNM RP
The sequence of this isoform differs from the canonical sequence as follows:
269-291: NPDGDAKPWCHVLKNRRLTWEYC → TGRSVSSPATASMRPCPLSIRSG
292-562: Missing.
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE7ESF4 | E7ESF4_HUMAN | Plasminogen activator | PLAT | 499 | Annotation score: | ||
B4DN26 | B4DN26_HUMAN | T-plasminogen activator | PLAT | 473 | Annotation score: | ||
A0A7I2YQ93 | A0A7I2YQ93_HUMAN | T-plasminogen activator | PLAT | 583 | Annotation score: | ||
B4DNJ1 | B4DNJ1_HUMAN | T-plasminogen activator | PLAT | 436 | Annotation score: | ||
A0A7I2V6D6 | A0A7I2V6D6_HUMAN | T-plasminogen activator | PLAT | 392 | Annotation score: | ||
E5RGA1 | E5RGA1_HUMAN | Tissue-type plasminogen activator | PLAT | 178 | Annotation score: | ||
E5RHG4 | E5RHG4_HUMAN | Tissue-type plasminogen activator | PLAT | 157 | Annotation score: | ||
A0A7I2V268 | A0A7I2V268_HUMAN | Tissue-type plasminogen activator | PLAT | 182 | Annotation score: | ||
H0YBH9 | H0YBH9_HUMAN | Tissue-type plasminogen activator | PLAT | 96 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 93 | N → T in AAB59510 (PubMed:6089198).Curated | 1 | |
Sequence conflicti | 159 – 160 | KP → NA in CAA31489 (PubMed:2107528).Curated | 2 | |
Sequence conflicti | 247 | K → N in AAO34406 (Ref. 9) Curated | 1 | |
Sequence conflicti | 283 | N → S in AAH95403 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 333 – 334 | RR → EE in AAK11956 (Ref. 8) Curated | 2 | |
Sequence conflicti | 389 | V → C in AAK11956 (Ref. 8) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_020181 | 34 | A → D1 PublicationCorresponds to variant dbSNP:rs8178733EnsemblClinVar. | 1 | |
Natural variantiVAR_038732 | 136 | R → S1 PublicationCorresponds to variant dbSNP:rs8178747Ensembl. | 1 | |
Natural variantiVAR_038733 | 146 | A → T1 PublicationCorresponds to variant dbSNP:rs8178748Ensembl. | 1 | |
Natural variantiVAR_011783 | 164 | R → W1 PublicationCorresponds to variant dbSNP:rs2020921Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028029 | 1 – 40 | MDAMK…SYQVI → MAS in isoform 4. 1 PublicationAdd BLAST | 40 | |
Alternative sequenceiVSP_015957 | 39 – 85 | VICRD…VPVKS → G in isoform 3. 2 PublicationsAdd BLAST | 47 | |
Alternative sequenceiVSP_028030 | 79 – 208 | Missing in isoform 4. 1 PublicationAdd BLAST | 130 | |
Alternative sequenceiVSP_005411 | 269 – 291 | NPDGD…TWEYC → TGRSVSSPATASMRPCPLSI RSG in isoform 2. 1 PublicationAdd BLAST | 23 | |
Alternative sequenceiVSP_005412 | 292 – 562 | Missing in isoform 2. 1 PublicationAdd BLAST | 271 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L00153 , L00141, L00142, L00143, L00144, L00145, L00146, L00147, L00148, L00149, L00150, L00151 Genomic DNA Translation: AAB59510.1 K03021 Genomic DNA Translation: AAA98809.1 M15518 mRNA Translation: AAA60111.1 M18182 mRNA Translation: AAA36800.1 X07393 mRNA Translation: CAA30302.1 X13097 mRNA Translation: CAA31489.1 AF260825 mRNA Translation: AAK11956.1 AY221101 mRNA Translation: AAO34406.1 AK289387 mRNA Translation: BAF82076.1 AK290575 mRNA Translation: BAF83264.1 AK313342 mRNA Translation: BAG36145.1 BT007060 mRNA Translation: AAP35709.1 AY291060 Genomic DNA Translation: AAP34246.1 CH471080 Genomic DNA Translation: EAW63235.1 CH471080 Genomic DNA Translation: EAW63233.1 BC002795 mRNA Translation: AAH02795.3 BC007231 mRNA Translation: AAH07231.1 BC013968 mRNA Translation: AAH13968.3 BC018636 mRNA Translation: AAH18636.3 BC095403 mRNA Translation: AAH95403.1 M11890, M11889 Genomic DNA Translation: AAA61213.1 D01096 mRNA Translation: BAA00881.1 V00570 mRNA Translation: CAA23833.1 |
CCDSi | CCDS6126.1 [P00750-1] CCDS6127.1 [P00750-3] |
PIRi | A94004, UKHUT I38098 |
RefSeqi | NP_000921.1, NM_000930.4 [P00750-1] NP_001306118.1, NM_001319189.1 NP_127509.1, NM_033011.3 [P00750-3] |
Genome annotation databases
Ensembli | ENST00000220809; ENSP00000220809; ENSG00000104368 ENST00000352041; ENSP00000270188; ENSG00000104368 [P00750-3] ENST00000429089; ENSP00000392045; ENSG00000104368 ENST00000679151; ENSP00000504311; ENSG00000104368 |
GeneIDi | 5327 |
KEGGi | hsa:5327 |
MANE-Selecti | ENST00000220809.9; ENSP00000220809.4; NM_000930.5; NP_000921.1 |
UCSCi | uc003xos.3, human [P00750-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Tissue plasminogen activator entry |
SeattleSNPs |
SHMPD The Singapore human mutation and polymorphism database |
Activase Clinical information on Activase |
Chiesi Clinical information on Retavase |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L00153 , L00141, L00142, L00143, L00144, L00145, L00146, L00147, L00148, L00149, L00150, L00151 Genomic DNA Translation: AAB59510.1 K03021 Genomic DNA Translation: AAA98809.1 M15518 mRNA Translation: AAA60111.1 M18182 mRNA Translation: AAA36800.1 X07393 mRNA Translation: CAA30302.1 X13097 mRNA Translation: CAA31489.1 AF260825 mRNA Translation: AAK11956.1 AY221101 mRNA Translation: AAO34406.1 AK289387 mRNA Translation: BAF82076.1 AK290575 mRNA Translation: BAF83264.1 AK313342 mRNA Translation: BAG36145.1 BT007060 mRNA Translation: AAP35709.1 AY291060 Genomic DNA Translation: AAP34246.1 CH471080 Genomic DNA Translation: EAW63235.1 CH471080 Genomic DNA Translation: EAW63233.1 BC002795 mRNA Translation: AAH02795.3 BC007231 mRNA Translation: AAH07231.1 BC013968 mRNA Translation: AAH13968.3 BC018636 mRNA Translation: AAH18636.3 BC095403 mRNA Translation: AAH95403.1 M11890, M11889 Genomic DNA Translation: AAA61213.1 D01096 mRNA Translation: BAA00881.1 V00570 mRNA Translation: CAA23833.1 |
CCDSi | CCDS6126.1 [P00750-1] CCDS6127.1 [P00750-3] |
PIRi | A94004, UKHUT I38098 |
RefSeqi | NP_000921.1, NM_000930.4 [P00750-1] NP_001306118.1, NM_001319189.1 NP_127509.1, NM_033011.3 [P00750-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A5H | X-ray | 2.90 | A/B | 311-562 | [»] | |
C/D | 298-304 | [»] | ||||
1BDA | X-ray | 3.35 | A/B | 298-562 | [»] | |
1PK2 | NMR | - | A | 209-298 | [»] | |
1PML | X-ray | 2.38 | A/B/C | 213-298 | [»] | |
1RTF | X-ray | 2.30 | B | 311-562 | [»] | |
1TPG | NMR | - | A | 36-126 | [»] | |
1TPK | X-ray | 2.40 | A/B/C | 211-298 | [»] | |
1TPM | NMR | - | A | 36-85 | [»] | |
1TPN | NMR | - | A | 36-85 | [»] | |
5BRR | X-ray | 3.16 | E | 311-562 | [»] | |
5ZLZ | X-ray | 3.58 | E | 311-561 | [»] | |
SMRi | P00750 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111343, 50 interactors |
ComplexPortali | CPX-494, tPA-PAI-1 complex |
ELMi | P00750 |
IntActi | P00750, 14 interactors |
MINTi | P00750 |
STRINGi | 9606.ENSP00000220809 |
Chemistry databases
BindingDBi | P00750 |
ChEMBLi | CHEMBL1873 |
DrugBanki | DB07684, 5-(DIMETHYLAMINO)-2-NAPHTHALENESULFONIC ACID DB00513, Aminocaproic acid DB09228, Conestat alfa DB09213, Dexibuprofen DB06404, Human C1-esterase inhibitor DB01088, Iloprost |
DrugCentrali | P00750 |
GuidetoPHARMACOLOGYi | 2392 |
Protein family/group databases
MEROPSi | S01.232 |
PTM databases
GlyConnecti | 603, 43 N-Linked glycans (3 sites), 1 O-Linked glycan (1 site) 86, 16 N-Linked glycans (1 site) |
GlyGeni | P00750, 6 sites, 103 N-linked glycans (4 sites), 1 O-linked glycan (1 site) |
iPTMneti | P00750 |
PhosphoSitePlusi | P00750 |
Genetic variation databases
BioMutai | PLAT |
DMDMi | 137119 |
Proteomic databases
CPTACi | non-CPTAC-1164 |
EPDi | P00750 |
jPOSTi | P00750 |
MassIVEi | P00750 |
MaxQBi | P00750 |
PaxDbi | P00750 |
PeptideAtlasi | P00750 |
PRIDEi | P00750 |
ProteomicsDBi | 51281 [P00750-1] 51282 [P00750-2] 51283 [P00750-3] 51284 [P00750-4] |
Protocols and materials databases
Antibodypediai | 998, 801 antibodies from 39 providers |
DNASUi | 5327 |
Genome annotation databases
Ensembli | ENST00000220809; ENSP00000220809; ENSG00000104368 ENST00000352041; ENSP00000270188; ENSG00000104368 [P00750-3] ENST00000429089; ENSP00000392045; ENSG00000104368 ENST00000679151; ENSP00000504311; ENSG00000104368 |
GeneIDi | 5327 |
KEGGi | hsa:5327 |
MANE-Selecti | ENST00000220809.9; ENSP00000220809.4; NM_000930.5; NP_000921.1 |
UCSCi | uc003xos.3, human [P00750-1] |
Organism-specific databases
CTDi | 5327 |
DisGeNETi | 5327 |
GeneCardsi | PLAT |
HGNCi | HGNC:9051, PLAT |
HPAi | ENSG00000104368, Tissue enhanced (parathyroid gland, urinary bladder) |
MalaCardsi | PLAT |
MIMi | 173370, gene |
neXtProti | NX_P00750 |
OpenTargetsi | ENSG00000104368 |
Orphaneti | 480528, Lethal hydranencephaly-diaphragmatic hernia syndrome 64738, NON RARE IN EUROPE: Non rare thrombophilia |
PharmGKBi | PA33381 |
VEuPathDBi | HostDB:ENSG00000104368 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000158930 |
HOGENOMi | CLU_006842_18_4_1 |
InParanoidi | P00750 |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P00750 |
TreeFami | TF329901 |
Enzyme and pathway databases
BRENDAi | 3.4.21.68, 2681 |
PathwayCommonsi | P00750 |
Reactomei | R-HSA-186797, Signaling by PDGF R-HSA-75205, Dissolution of Fibrin Clot |
SignaLinki | P00750 |
SIGNORi | P00750 |
Miscellaneous databases
BioGRID-ORCSi | 5327, 7 hits in 1047 CRISPR screens |
ChiTaRSi | PLAT, human |
EvolutionaryTracei | P00750 |
GeneWikii | Tissue_plasminogen_activator |
GenomeRNAii | 5327 |
Pharosi | P00750, Tclin |
PROi | PR:P00750 |
RNActi | P00750, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000104368, Expressed in stromal cell of endometrium and 228 other tissues |
ExpressionAtlasi | P00750, baseline and differential |
Genevisiblei | P00750, HS |
Family and domain databases
CDDi | cd00061, FN1, 1 hit cd00108, KR, 2 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.40.20.10, 2 hits |
InterProi | View protein in InterPro IPR000742, EGF-like_dom IPR000083, Fibronectin_type1 IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR026280, Tissue_plasm_act IPR034811, tPA IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24264:SF42, PTHR24264:SF42, 3 hits |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00039, fn1, 1 hit PF00051, Kringle, 2 hits PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001145, Tissue_plasm_act, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00058, FN1, 1 hit SM00130, KR, 2 hits SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS01253, FN1_1, 1 hit PS51091, FN1_2, 1 hit PS00021, KRINGLE_1, 2 hits PS50070, KRINGLE_2, 2 hits PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TPA_HUMAN | |
Accessioni | P00750Primary (citable) accession number: P00750 Secondary accession number(s): A8K022 Q9BZW1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 262 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Pharmaceutical, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families