Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endolysin

Gene

E

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.UniRule annotation1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.UniRule annotation1 Publication

pH dependencei

Optimum pH is 7.3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11Proton donorUniRule annotation1 Publication1
Active sitei20NucleophileUniRule annotation2 Publications1
Binding sitei32N-acetyl-D-glucosamine; via amide nitrogenUniRule annotation1 Publication1
Binding sitei104N-acetyl-D-glucosamine; via amide nitrogenUniRule annotation1 Publication1
Sitei117Substrate-binding1 Publication1
Sitei132Substrate-binding1 Publication1

GO - Molecular functioni

  • lysozyme activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase
Biological processCytolysis, Host cell lysis by virus, Viral release from host cell

Enzyme and pathway databases

BRENDAi3.2.1.17 732

Protein family/group databases

CAZyiGH24 Glycoside Hydrolase Family 24

Names & Taxonomyi

Protein namesi
Recommended name:
EndolysinUniRule annotation (EC:3.2.1.17UniRule annotation1 Publication)
Alternative name(s):
Lysis proteinUniRule annotation
LysozymeUniRule annotation
MuramidaseUniRule annotation
Gene namesi
Name:E
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation
  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi11E → A, F, H, M or N: Almost complete loss of enzymatic activity. 1 Publication1
Mutagenesisi11E → N: Loss of 84% of enzymatic activity. 1 Publication1
Mutagenesisi11E → Q: Complete loss of activity. 1 Publication1
Mutagenesisi20D → A, N, S or T: Almost complete loss of enzymatic activity. 1 Publication1
Mutagenesisi20D → C: Nearly no effet on specific enzymatic activity. 1 Publication1
Mutagenesisi20D → E or Q: Loss of 99% of enzymatic activity. 1 Publication1
Mutagenesisi26T → E: Covalently bound substrate. 1 Publication1
Mutagenesisi30G → A: Almost complete loss of enzymatic activity. 1 Publication1
Mutagenesisi30G → F: Almost complete loss of enzymatic activity. The enzyme is destabilized by 1.5 kcal/ mol. 1 Publication1
Mutagenesisi117S → F: 10-fold decrease in enzymatic activity. 1 Publication1
Mutagenesisi117S → I: 500-fold decrease in enzymatic activity. 1 Publication1
Mutagenesisi117S → V: 50-fold decrease in enzymatic activity. 1 Publication1
Mutagenesisi132N → I: 5-fold decrease in enzymatic activity. 1 Publication1
Mutagenesisi132N → M or F: 2-fold decrease in enzymatic activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5206
DrugBankiDB06934 (1R)-3-chloro-1-phenylpropan-1-ol
DB07543 (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol
DB03301 2-Allyl-6-Methyl-Phenol
DB02534 2-Allylphenol
DB03842 2-Chloro-6-Methyl-Aniline
DB02403 2-Fluoroaniline
DB02486 2-Hydroxyethyl Disulfide
DB02970 2-Propyl-Aniline
DB01957 3-Chlorophenol
DB01986 3-Fluoro-2-Methyl-Aniline
DB03669 4-Fluorophenethyl Alcohol
DB03660 4-Iodo-L-phenylalanine
DB01932 5-Methylpyrrole
DB04179 Benzofuran
DB03345 Beta-Mercaptoethanol
DB00536 Guanidine
DB04532 Indole
DB02870 N-Allyl-Aniline
DB03255 Phenol
DB00373 Timolol

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002181071 – 164EndolysinAdd BLAST164

Expressioni

Inductioni

Expressed late in the viral replication.

Structurei

Secondary structure

1164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiP00720
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00720

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000CE

Family and domain databases

HAMAPiMF_04110 ENDOLYSIN_T4, 1 hit
InterProiView protein in InterPro
IPR034690 Endolysin_T4_type
IPR002196 Glyco_hydro_24
IPR023346 Lysozyme-like_dom_sf
IPR001165 T4-type_lysozyme
PfamiView protein in Pfam
PF00959 Phage_lysozyme, 1 hit
PRINTSiPR00684 T4LYSOZYME
SUPFAMiSSF53955 SSF53955, 1 hit

Sequencei

Sequence statusi: Complete.

P00720-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNIFEMLRID ERLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI
60 70 80 90 100
GRNCNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRCALI
110 120 130 140 150
NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSIWYN QTPNRAKRVI
160
TTFRTGTWDA YKNL
Length:164
Mass (Da):18,692
Last modified:May 14, 2014 - v2
Checksum:i02D568CC8FC81CF1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12R → G AA sequence (PubMed:5470817).Curated1
Sequence conflicti137I → R AA sequence (PubMed:5470817).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04567 Genomic DNA Translation: CAA28212.1
AF158101 Genomic DNA Translation: AAD42568.1
PIRiA92896 LZBPT4
RefSeqiNP_049736.1, NC_000866.4

Genome annotation databases

GeneIDi1258585
KEGGivg:1258585

Similar proteinsi

Entry informationi

Entry nameiENLYS_BPT4
AccessioniPrimary (citable) accession number: P00720
Secondary accession number(s): Q38170, Q94N07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 14, 2014
Last modified: September 12, 2018
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again