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Protein

Alpha-amylase

Gene
N/A
Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi133Calcium 1Combined sources2 Publications1
Metal bindingi190Calcium 2Combined sources2 Publications1
Metal bindingi190SodiumCombined sources1 Publication1
Metal bindingi212Calcium 2; via carbonyl oxygenCombined sources2 Publications1
Metal bindingi214Calcium 2Combined sources2 Publications1
Metal bindingi214SodiumCombined sources1 Publication1
Metal bindingi225Calcium 1Combined sources2 Publications1
Metal bindingi225SodiumCombined sources1 Publication1
Metal bindingi231Calcium 1Combined sources2 Publications1
Metal bindingi231SodiumCombined sources1 Publication1
Metal bindingi233Calcium 2Combined sources2 Publications1
Metal bindingi235Calcium 2Combined sources2 Publications1
Active sitei262Nucleophile1
Metal bindingi266Calcium 1; via carbonyl oxygenCombined sources2 Publications1
Active sitei292Proton donor1
Metal bindingi331Calcium 3; via carbonyl oxygenCombined sources1 Publication1
Sitei359Transition state stabilizerBy similarity1
Metal bindingi438Calcium 3Combined sources1 Publication1
Metal bindingi461Calcium 3Combined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.1.1 630

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

DrugBankiDB03773 6-Deoxy-Alpha-D-Glucose
DB02379 Beta-D-Glucose

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 311 PublicationAdd BLAST31
ChainiPRO_000000133032 – 514Alpha-amylaseAdd BLAST483

Proteomic databases

PRIDEiP00692

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1514
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00692
SMRiP00692
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00692

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR013776 A-amylase_thermo
IPR015237 Alpha-amylase_C_pro
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF09154 DUF1939, 1 hit
PIRSFiPIRSF001021 Alph-amls_thrmst, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00692-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIQKRKRTVS FRLVLMCTLL FVSLPITKTS AVNGTLMQYF EWYTPNDGQH
60 70 80 90 100
WKRLQNDAEH LSDIGITAVW IPPAYKGLSQ SDNGYGPYDL YDLGEFQQKG
110 120 130 140 150
TVRTKYGTKS ELQDAIGSLH SRNVQVYGDV VLNHKAGADA TEDVTAVEVN
160 170 180 190 200
PANRNQETSE EYQIKAWTDF RFPGRGNTYS DFKWHWYHFD GADWDESRKI
210 220 230 240 250
SRIFKFRGEG KAWDWEVSSE NGNYDYLMYA DVDYDHPDVV AETKKWGIWY
260 270 280 290 300
ANELSLDGFR IDAAKHIKFS FLRDWVQAVR QATGKEMFTV AEYWQNNAGK
310 320 330 340 350
LENYLNKTSF NQSVFDVPLH FNLQAASSQG GGYDMRRLLD GTVVSRHPEK
360 370 380 390 400
AVTFVENHDT QPGQSLESTV QTWFKPLAYA FILTRESGYP QVFYGDMYGT
410 420 430 440 450
KGTSPKEIPS LKDNIEPILK ARKEYAYGPQ HDYIDHPDVI GWTREGDSSA
460 470 480 490 500
AKSGLAALIT DGPGGSKRMY AGLKNAGETW YDITGNRSDT VKIGSDGWGE
510
FHVNDGSVSI YVQK
Length:514
Mass (Da):58,403
Last modified:January 1, 1988 - v1
Checksum:i3DE66B3FB5CCDE7E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54L → I AA sequence (PubMed:6156671).Curated1
Sequence conflicti64I → L AA sequence (PubMed:6156671).Curated1
Sequence conflicti79S → D AA sequence (PubMed:6156671).Curated1
Sequence conflicti84G → S AA sequence (PubMed:6156671).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01542 mRNA Translation: AAA22191.1
V00092 Genomic DNA Translation: CAA23430.1
M18424 Genomic DNA Translation: AAA22192.1
PIRiA92389 ALBSN

Genome annotation databases

GeneIDi9780104

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01542 mRNA Translation: AAA22191.1
V00092 Genomic DNA Translation: CAA23430.1
M18424 Genomic DNA Translation: AAA22192.1
PIRiA92389 ALBSN

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3XX-ray1.90A32-331[»]
1E3ZX-ray1.93A32-331[»]
1E40X-ray2.20A32-331[»]
1E43X-ray1.70A32-331[»]
3BH4X-ray1.40A/B32-514[»]
ProteinModelPortaliP00692
SMRiP00692
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB03773 6-Deoxy-Alpha-D-Glucose
DB02379 Beta-D-Glucose

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Proteomic databases

PRIDEiP00692

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9780104

Enzyme and pathway databases

BRENDAi3.2.1.1 630

Miscellaneous databases

EvolutionaryTraceiP00692

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR013776 A-amylase_thermo
IPR015237 Alpha-amylase_C_pro
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF09154 DUF1939, 1 hit
PIRSFiPIRSF001021 Alph-amls_thrmst, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY_BACAM
AccessioniPrimary (citable) accession number: P00692
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1988
Last modified: February 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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