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Protein

Alpha-amylase

Gene

amyE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Ca2+1 PublicationNote: Binds 2 calcium ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi142Calcium 11 Publication1
Metal bindingi178Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi187Calcium 11 Publication1
Metal bindingi210Calcium 2; via carbonyl oxygen1 Publication1
Metal bindingi212Calcium 21 Publication1
Active sitei217Nucleophile1 Publication1
Metal bindingi221Calcium 1; via carbonyl oxygen1 Publication1
Active sitei249Proton donor1 Publication1
Sitei310Transition state stabilizer1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU03040-MONOMER
SABIO-RKiP00691

Protein family/group databases

CAZyiCBM26 Carbohydrate-Binding Module Family 26
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:amyE
Synonyms:amyA
Ordered Locus Names:BSU03040
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3739249
DrugBankiDB02379 Beta-D-Glucose

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Add BLAST27
PropeptideiPRO_000000133528 – 41Add BLAST14
ChainiPRO_000000133642 – 659Alpha-amylaseAdd BLAST618

Proteomic databases

PRIDEiP00691

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1659
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00691
SMRiP00691
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00691

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000008732
InParanoidiP00691
KOiK01176
OMAiFHNAMVG

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR031965 CBM26
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF16738 CBM26, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00691-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFAKRFKTSL LPLFAGFLLL FHLVLAGPAA ASAETANKSN ELTAPSIKSG
60 70 80 90 100
TILHAWNWSF NTLKHNMKDI HDAGYTAIQT SPINQVKEGN QGDKSMSNWY
110 120 130 140 150
WLYQPTSYQI GNRYLGTEQE FKEMCAAAEE YGIKVIVDAV INHTTSDYAA
160 170 180 190 200
ISNEVKSIPN WTHGNTQIKN WSDRWDVTQN SLLGLYDWNT QNTQVQSYLK
210 220 230 240 250
RFLDRALNDG ADGFRFDAAK HIELPDDGSY GSQFWPNITN TSAEFQYGEI
260 270 280 290 300
LQDSASRDAA YANYMDVTAS NYGHSIRSAL KNRNLGVSNI SHYASDVSAD
310 320 330 340 350
KLVTWVESHD TYANDDEEST WMSDDDIRLG WAVIASRSGS TPLFFSRPEG
360 370 380 390 400
GGNGVRFPGK SQIGDRGSAL FEDQAITAVN RFHNVMAGQP EELSNPNGNN
410 420 430 440 450
QIFMNQRGSH GVVLANAGSS SVSINTATKL PDGRYDNKAG AGSFQVNDGK
460 470 480 490 500
LTGTINARSV AVLYPDDIAK APHVFLENYK TGVTHSFNDQ LTITLRADAN
510 520 530 540 550
TTKAVYQINN GPETAFKDGD QFTIGKGDPF GKTYTIMLKG TNSDGVTRTE
560 570 580 590 600
KYSFVKRDPA SAKTIGYQNP NHWSQVNAYI YKHDGSRVIE LTGSWPGKPM
610 620 630 640 650
TKNADGIYTL TLPADTDTTN AKVIFNNGSA QVPGQNQPGF DYVLNGLYND

SGLSGSLPH
Length:659
Mass (Da):72,378
Last modified:June 16, 2009 - v2
Checksum:i16BD6CB1E7C67BD6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22H → Y in CAA26086 (PubMed:6099357).Curated1
Sequence conflicti22H → Y in AAA22234 (PubMed:6413492).Curated1
Sequence conflicti22H → Y in CAA23436 (PubMed:6189486).Curated1
Sequence conflicti22H → Y in AAA22230 (Ref. 8) Curated1
Sequence conflicti146S → F in CAA26086 (PubMed:6099357).Curated1
Sequence conflicti146S → F in AAA22234 (PubMed:6413492).Curated1
Sequence conflicti204D → E in CAA26086 (PubMed:6099357).Curated1
Sequence conflicti204D → E in AAA22234 (PubMed:6413492).Curated1
Sequence conflicti284N → K in CAA26086 (PubMed:6099357).Curated1
Sequence conflicti332A → S in AAA22234 (PubMed:6413492).Curated1
Sequence conflicti513 – 529ETAFK…GKGDP → DDRRLRMEINSQSEKEIQ in CAA23437 (PubMed:6186986).CuratedAdd BLAST17
Sequence conflicti513 – 529ETAFK…GKGDP → DDRRLRMEINSQSEKEIQ in BAA08938 (PubMed:8969502).CuratedAdd BLAST17

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti469 – 477AKAPHVFLE → EMRCNTFFQ in strain: N7 AMYEN+. 9
Natural varianti478 – 659Missing in strain: N7 AMYEN+. Add BLAST182

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00101 Genomic DNA Translation: CAA23437.1
V00100 Genomic DNA Translation: CAA23436.1
D50453 Genomic DNA Translation: BAA08938.1
AL009126 Genomic DNA Translation: CAB12098.2
K00563 Genomic DNA Translation: AAA22234.1
X02150 Genomic DNA Translation: CAA26086.1
M35517 Genomic DNA Translation: AAA22230.1
PIRiA00842 ALBS
A91793 ALBSNA
RefSeqiNP_388186.2, NC_000964.3
WP_003234692.1, NZ_JNCM01000030.1

Genome annotation databases

EnsemblBacteriaiCAB12098; CAB12098; BSU03040
GeneIDi938356
KEGGibsu:BSU03040
PATRICifig|224308.179.peg.317

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00101 Genomic DNA Translation: CAA23437.1
V00100 Genomic DNA Translation: CAA23436.1
D50453 Genomic DNA Translation: BAA08938.1
AL009126 Genomic DNA Translation: CAB12098.2
K00563 Genomic DNA Translation: AAA22234.1
X02150 Genomic DNA Translation: CAA26086.1
M35517 Genomic DNA Translation: AAA22230.1
PIRiA00842 ALBS
A91793 ALBSNA
RefSeqiNP_388186.2, NC_000964.3
WP_003234692.1, NZ_JNCM01000030.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BAGX-ray2.50A42-466[»]
1UA7X-ray2.21A45-466[»]
ProteinModelPortaliP00691
SMRiP00691
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

ChEMBLiCHEMBL3739249
DrugBankiDB02379 Beta-D-Glucose

Protein family/group databases

CAZyiCBM26 Carbohydrate-Binding Module Family 26
GH13 Glycoside Hydrolase Family 13

Proteomic databases

PRIDEiP00691

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12098; CAB12098; BSU03040
GeneIDi938356
KEGGibsu:BSU03040
PATRICifig|224308.179.peg.317

Phylogenomic databases

HOGENOMiHOG000008732
InParanoidiP00691
KOiK01176
OMAiFHNAMVG

Enzyme and pathway databases

BioCyciBSUB:BSU03040-MONOMER
SABIO-RKiP00691

Miscellaneous databases

EvolutionaryTraceiP00691

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR031965 CBM26
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF16738 CBM26, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY_BACSU
AccessioniPrimary (citable) accession number: P00691
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  4. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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Main funding by: National Institutes of Health

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