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Protein

Pancreatic alpha-amylase

Gene

AMY2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

The two forms of this enzyme, I and II, show very similar activities, molecular masses, and compositions and differ only in their isoelectric points. As no evidence for two variants were in the cDNA library of PubMed:10082956, it is most likely that isoform I (PPAI) and isoform II (PPAII) are two forms of the same protein.

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115Calcium8 Publications1
Metal bindingi173Calcium; via carbonyl oxygen8 Publications1
Metal bindingi182Calcium8 Publications1
Binding sitei210Chloride5 Publications1
Active sitei212Nucleophile1
Metal bindingi216Calcium; via carbonyl oxygen8 Publications1
Active sitei248Proton donor1
Binding sitei313ChlorideCurated1
Sitei315Transition state stabilizerBy similarity1
Binding sitei352Chloride5 Publications1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.1.1 6170
SABIO-RKiP00690

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic alpha-amylase (EC:3.2.1.1By similarity)
Short name:
PA
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:AMY2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei970 Sus s Amylase

Chemistry databases

ChEMBLiCHEMBL5730

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 151 PublicationAdd BLAST15
ChainiPRO_000000139916 – 511Pancreatic alpha-amylaseAdd BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi43 ↔ 1011 Publication8 Publications
Disulfide bondi85 ↔ 1301 Publication8 Publications
Disulfide bondi156 ↔ 1751 Publication8 Publications
Disulfide bondi393 ↔ 3991 Publication8 Publications
Glycosylationi427N-linked (GlcNAc...) asparagine1
Disulfide bondi465 ↔ 4771 Publication8 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP00690
PeptideAtlasiP00690
PRIDEiP00690

Interactioni

Protein-protein interaction databases

MINTiP00690
STRINGi9823.ENSSSCP00000026998

Chemistry databases

BindingDBiP00690

Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00690
SMRiP00690
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00690

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP00690
KOiK01176

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00690-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLFLLLSAF GFCWAQYAPQ TQSGRTSIVH LFEWRWVDIA LECERYLGPK
60 70 80 90 100
GFGGVQVSPP NENIVVTNPS RPWWERYQPV SYKLCTRSGN ENEFRDMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGS GAAAGTGTTC GSYCNPGNRE FPAVPYSAWD
160 170 180 190 200
FNDGKCKTAS GGIESYNDPY QVRDCQLVGL LDLALEKDYV RSMIADYLNK
210 220 230 240 250
LIDIGVAGFR IDASKHMWPG DIKAVLDKLH NLNTNWFPAG SRPFIFQEVI
260 270 280 290 300
DLGGEAIQSS EYFGNGRVTE FKYGAKLGTV VRKWSGEKMS YLKNWGEGWG
310 320 330 340 350
FMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKVAV GFMLAHPYGF
360 370 380 390 400
TRVMSSYRWA RNFVNGQDVN DWIGPPNNNG VIKEVTINAD TTCGNDWVCE
410 420 430 440 450
HRWRQIRNMV WFRNVVDGQP FANWWANGSN QVAFGRGNRG FIVFNNDDWQ
460 470 480 490 500
LSSTLQTGLP GGTYCDVISG DKVGNSCTGI KVYVSSDGTA QFSISNSAED
510
PFIAIHAESK L
Length:511
Mass (Da):57,086
Last modified:August 15, 2003 - v3
Checksum:i117489E020807378
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138N → S AA sequence (PubMed:3484639).Curated1
Sequence conflicti367Q → E AA sequence (PubMed:3484639).Curated1
Sequence conflicti405Q → E AA sequence (PubMed:3484639).Curated1
Sequence conflicti419Q → E AA sequence (PubMed:3484639).Curated1
Sequence conflicti426A → D AA sequence (PubMed:3484639).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064742 mRNA Translation: AAF02828.1
PIRiA25412 ALPGP
RefSeqiNP_999360.1, NM_214195.1
UniGeneiSsc.94418

Genome annotation databases

GeneIDi397397
KEGGissc:397397

Similar proteinsi

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064742 mRNA Translation: AAF02828.1
PIRiA25412 ALPGP
RefSeqiNP_999360.1, NM_214195.1
UniGeneiSsc.94418

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BVNX-ray2.50P16-511[»]
1DHKX-ray1.85A17-511[»]
1HX0X-ray1.38A16-511[»]
1JFHX-ray2.03A17-511[»]
1KXQX-ray1.60A/B/C/D16-511[»]
1KXTX-ray2.00A/C/E16-511[»]
1KXVX-ray1.60A/B16-511[»]
1OSEX-ray2.30A17-511[»]
1PIFX-ray2.30A17-511[»]
1PIGX-ray2.20A17-511[»]
1PPIX-ray2.20A16-511[»]
1UA3X-ray2.01A16-511[»]
1VAHX-ray2.40A16-511[»]
1WO2X-ray2.01A16-511[»]
3L2LX-ray2.11A16-511[»]
3L2MX-ray1.97A16-511[»]
4X0NX-ray2.60A17-511[»]
ProteinModelPortaliP00690
SMRiP00690
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiP00690
STRINGi9823.ENSSSCP00000026998

Chemistry databases

BindingDBiP00690
ChEMBLiCHEMBL5730

Protein family/group databases

Allergomei970 Sus s Amylase
CAZyiGH13 Glycoside Hydrolase Family 13

Proteomic databases

PaxDbiP00690
PeptideAtlasiP00690
PRIDEiP00690

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397397
KEGGissc:397397

Organism-specific databases

CTDi397397

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP00690
KOiK01176

Enzyme and pathway databases

BRENDAi3.2.1.1 6170
SABIO-RKiP00690

Miscellaneous databases

EvolutionaryTraceiP00690
PROiPR:P00690

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMYP_PIG
AccessioniPrimary (citable) accession number: P00690
Secondary accession number(s): Q9TUE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 15, 2003
Last modified: September 12, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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