UniProtKB - P00688 (AMYP_MOUSE)
Protein
Pancreatic alpha-amylase
Gene
Amy2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalytic activityi
- Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity EC:3.2.1.1
Cofactori
Protein has several cofactor binding sites:- Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
- chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 115 | CalciumBy similarity | 1 | |
Metal bindingi | 170 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 179 | CalciumBy similarity | 1 | |
Binding sitei | 207 | ChlorideBy similarity | 1 | |
Active sitei | 209 | NucleophileBy similarity | 1 | |
Metal bindingi | 213 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 245 | Proton donorBy similarity | 1 | |
Binding sitei | 310 | ChlorideBy similarity | 1 | |
Sitei | 312 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 349 | ChlorideBy similarity | 1 |
GO - Molecular functioni
- alpha-amylase activity Source: UniProtKB
- alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
- amylase activity Source: MGI
- calcium ion binding Source: UniProtKB
- chloride ion binding Source: UniProtKB
GO - Biological processi
- carbohydrate catabolic process Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Calcium, Chloride, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-189085, Digestion of dietary carbohydrate |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
Names & Taxonomyi
Protein namesi | Recommended name: Pancreatic alpha-amylase (EC:3.2.1.1By similarity)Short name: PA Alternative name(s): 1,4-alpha-D-glucan glucanohydrolase |
Gene namesi | Name:Amy2 Synonyms:Amy2a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88020, Amy2 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | 1 PublicationAdd BLAST | 15 | |
ChainiPRO_0000001398 | 16 – 508 | Pancreatic alpha-amylaseAdd BLAST | 493 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 16 | Pyrrolidone carboxylic acid1 Publication | 1 | |
Disulfide bondi | 43 ↔ 101 | By similarity | ||
Disulfide bondi | 85 ↔ 130 | By similarity | ||
Disulfide bondi | 156 ↔ 172 | By similarity | ||
Disulfide bondi | 390 ↔ 396 | By similarity | ||
Disulfide bondi | 462 ↔ 474 | By similarity |
Keywords - PTMi
Disulfide bond, Pyrrolidone carboxylic acidProteomic databases
CPTACi | non-CPTAC-3891 |
jPOSTi | P00688 |
MaxQBi | P00688 |
PaxDbi | P00688 |
PeptideAtlasi | P00688 |
PRIDEi | P00688 |
2D gel databases
SWISS-2DPAGEi | P00688 |
PTM databases
iPTMneti | P00688 |
PhosphoSitePlusi | P00688 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000074268, Expressed in pancreas and 29 other tissues |
Genevisiblei | P00688, MM |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000137025 |
Miscellaneous databases
RNActi | P00688, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 13 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2212, Eukaryota |
GeneTreei | ENSGT00940000163532 |
HOGENOMi | CLU_013336_2_1_1 |
InParanoidi | P00688 |
OMAi | SGWICEH |
OrthoDBi | 665362at2759 |
TreeFami | TF312850 |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR031319, A-amylase_C IPR006046, Alpha_amylase IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit |
PRINTSi | PR00110, ALPHAAMYLASE |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit SM00632, Aamy_C, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00688-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFVLLLSLI GFCWAQYDPH TSDGRTAIVH LFEWRWVDIA KECERYLAPK
60 70 80 90 100
GFGGVQVSPP NENVVVHNPS RPWWERYQPI SYKICTRSGN EDEFRDMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGA GNPAGTSSTC GSYLNPNNRE FPAVPYSAWD
160 170 180 190 200
FNDNKCNGEI DNYNDAYQVR NCRLTGLLDL ALEKDYVRTK VADYMNHLID
210 220 230 240 250
IGVAGFRLDA AKHMWPGDIK AVLDKLHNLN TKWFSQGSRP FIFQEVIDLG
260 270 280 290 300
GEAIKGSEYF GNGRVTEFKY GAKLGTVIRK WNGEKMSYLK NWGEGWGLVP
310 320 330 340 350
SDRALVFVDN HDNQRGHGAG GSSILTFWDA RMYKMAVGFM LAHPYGFTRV
360 370 380 390 400
MSSYRWNRNF QNGKDQNDWI GPPNNNGVTK EVTINADTTC GNDWVCEHRW
410 420 430 440 450
RQIRNMVAFR NVVNGQPFSN WWDNNSNQVA FSRGNRGFIV FNNDDWALSA
460 470 480 490 500
TLQTGLPAGT YCDVISGDKV DGNCTGLRVN VGSDGKAHFS ISNSAEDPFI
AIHADSKL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 70 | S → T in AAA37228 (PubMed:6176715).Curated | 1 | |
Sequence conflicti | 85 | C → S in AAA37228 (PubMed:6176715).Curated | 1 | |
Sequence conflicti | 217 | G → R in CAA24098 (PubMed:6157477).Curated | 1 | |
Sequence conflicti | 217 | G → R in CAA26413 (PubMed:6098446).Curated | 1 | |
Sequence conflicti | 217 | G → R in CAA26414 (PubMed:6098446).Curated | 1 | |
Sequence conflicti | 217 | G → R in CAA26415 (PubMed:6098446).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 64 | V → I in A1, B(C) and AMY-2.2Y. | 1 | |
Natural varianti | 66 | V → I in AMY-2.2Y. | 1 | |
Natural varianti | 120 | A → S in A1, B(A) and B(C). | 1 | |
Natural varianti | 161 | D → S in A1, B(A) and B(C); requires 2 nucleotide substitutions. | 1 | |
Natural varianti | 174 | L → V in A1 and B(C). | 1 | |
Natural varianti | 175 | T → S in B(A). | 1 | |
Natural varianti | 254 | I → V in A1 and B(C). | 1 | |
Natural varianti | 270 – 272 | YGA → FGV in A1 and B(C). | 3 | |
Natural varianti | 298 | L → M in A1, B(A) and B(C). Combined sources | 1 | |
Natural varianti | 322 | S → A in B(A). | 1 | |
Natural varianti | 419 | S → A in B1. | 1 | |
Natural varianti | 450 | A → E in B(C). | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00718 mRNA Translation: CAA24098.1 X02576 mRNA Translation: CAA26413.1 X02577 mRNA Translation: CAA26414.1 X02578 mRNA Translation: CAA26415.1 AK133526 mRNA Translation: BAE21706.1 BC094924 mRNA Translation: AAH94924.1 BC100579 mRNA Translation: AAI00580.1 J00357 Genomic DNA Translation: AAA37228.1 J00358 Genomic DNA Translation: AAA37229.1 J00361 Genomic DNA Translation: AAA37233.1 X02343 Genomic DNA Translation: CAA26202.1 M16540 Genomic DNA Translation: AAA37223.1 M15965 Genomic DNA Translation: AAA37226.1 M11895 mRNA Translation: AAA37224.1 M11896 mRNA Translation: AAA37227.1 M11891 Genomic DNA Translation: AAA37222.1 |
CCDSi | CCDS17775.1 |
PIRi | A90995, ALMSP1 B90798, ALMSP B90995, ALMSPC C90995, ALMSPA I49493 I49494 |
RefSeqi | NP_001036176.1, NM_001042711.2 NP_001153622.1, NM_001160150.1 NP_001153623.1, NM_001160151.1 NP_001153624.1, NM_001160152.1 |
Genome annotation databases
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00718 mRNA Translation: CAA24098.1 X02576 mRNA Translation: CAA26413.1 X02577 mRNA Translation: CAA26414.1 X02578 mRNA Translation: CAA26415.1 AK133526 mRNA Translation: BAE21706.1 BC094924 mRNA Translation: AAH94924.1 BC100579 mRNA Translation: AAI00580.1 J00357 Genomic DNA Translation: AAA37228.1 J00358 Genomic DNA Translation: AAA37229.1 J00361 Genomic DNA Translation: AAA37233.1 X02343 Genomic DNA Translation: CAA26202.1 M16540 Genomic DNA Translation: AAA37223.1 M15965 Genomic DNA Translation: AAA37226.1 M11895 mRNA Translation: AAA37224.1 M11896 mRNA Translation: AAA37227.1 M11891 Genomic DNA Translation: AAA37222.1 |
CCDSi | CCDS17775.1 |
PIRi | A90995, ALMSP1 B90798, ALMSP B90995, ALMSPC C90995, ALMSPA I49493 I49494 |
RefSeqi | NP_001036176.1, NM_001042711.2 NP_001153622.1, NM_001160150.1 NP_001153623.1, NM_001160151.1 NP_001153624.1, NM_001160152.1 |
3D structure databases
SMRi | P00688 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000137025 |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
PTM databases
iPTMneti | P00688 |
PhosphoSitePlusi | P00688 |
2D gel databases
SWISS-2DPAGEi | P00688 |
Proteomic databases
CPTACi | non-CPTAC-3891 |
jPOSTi | P00688 |
MaxQBi | P00688 |
PaxDbi | P00688 |
PeptideAtlasi | P00688 |
PRIDEi | P00688 |
Protocols and materials databases
DNASUi | 109959 |
Genome annotation databases
Organism-specific databases
CTDi | 100043684 100043686 100043688 109959 |
MGIi | MGI:88020, Amy2 |
Phylogenomic databases
eggNOGi | KOG2212, Eukaryota |
GeneTreei | ENSGT00940000163532 |
HOGENOMi | CLU_013336_2_1_1 |
InParanoidi | P00688 |
OMAi | SGWICEH |
OrthoDBi | 665362at2759 |
TreeFami | TF312850 |
Enzyme and pathway databases
Reactomei | R-MMU-189085, Digestion of dietary carbohydrate |
Miscellaneous databases
BioGRID-ORCSi | 100043684, 3 hits in 11 CRISPR screens 100043686, 4 hits in 13 CRISPR screens 100043688, 2 hits in 12 CRISPR screens 109959, 3 hits in 11 CRISPR screens |
PROi | PR:P00688 |
RNActi | P00688, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000074268, Expressed in pancreas and 29 other tissues |
Genevisiblei | P00688, MM |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR031319, A-amylase_C IPR006046, Alpha_amylase IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit |
PRINTSi | PR00110, ALPHAAMYLASE |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit SM00632, Aamy_C, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMYP_MOUSE | |
Accessioni | P00688Primary (citable) accession number: P00688 Secondary accession number(s): Q4VBW6 Q64301 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries