Amy2

Functionⁱ

Catalytic activityⁱ

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactorⁱ

Protein has several cofactor binding sites:

Ca²⁺
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:P04746 (AMYP_HUMAN)
Note: Binds 1 Ca²⁺ ion per subunit.
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:P04746 (AMYP_HUMAN)
chloride
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:P04746 (AMYP_HUMAN)
Note: Binds 1 Cl^- ion per subunit.
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:P04746 (AMYP_HUMAN)

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	115	CalciumBy similarity	1
Metal bindingⁱ	170	Calcium; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	179	CalciumBy similarity	1
Binding siteⁱ	207	ChlorideBy similarity	1
Active siteⁱ	209	NucleophileBy similarity	1
Metal bindingⁱ	213	Calcium; via carbonyl oxygenBy similarity	1
Active siteⁱ	245	Proton donorBy similarity	1
Binding siteⁱ	310	ChlorideBy similarity	1
Siteⁱ	312	Transition state stabilizerBy similarity	1
Binding siteⁱ	349	ChlorideBy similarity	1

GO - Molecular functionⁱ

alpha-amylase activity Source: UniProtKB
alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
amylase activity
Source: MGI
Inferred from direct assayⁱ
- 6163812
calcium ion binding Source: UniProtKB
chloride ion binding Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

carbohydrate catabolic process Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosidase, Hydrolase
Biological process	Carbohydrate metabolism
Ligand	Calcium, Chloride, Metal-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-189085 Digestion of dietary carbohydrate

Reactomeⁱ

R-MMU-189085 Digestion of dietary carbohydrate

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH13 Glycoside Hydrolase Family 13

CAZyⁱ

GH13 Glycoside Hydrolase Family 13

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Pancreatic alpha-amylase (EC:3.2.1.1By similarity) Short name: PA Alternative name(s): 1,4-alpha-D-glucan glucanohydrolase
Gene namesⁱ	Name:Amy2 Synonyms:Amy2a
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 3

Organism-specific databases

MGIⁱ	MGI:88020 Amy2

Keywords - Cellular componentⁱ

Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 15	1 PublicationAdd BLAST		15
ChainⁱPRO_0000001398	16 – 508	Pancreatic alpha-amylaseAdd BLAST		493

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	16	Pyrrolidone carboxylic acid1 Publication	1
Disulfide bondⁱ	43 ↔ 101	By similarity
Disulfide bondⁱ	85 ↔ 130	By similarity
Disulfide bondⁱ	156 ↔ 172	By similarity
Disulfide bondⁱ	390 ↔ 396	By similarity
Disulfide bondⁱ	462 ↔ 474	By similarity

Keywords - PTMⁱ

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P00688
PaxDbⁱ	P00688
PeptideAtlas More...PeptideAtlasⁱ	P00688
PRIDEⁱ	P00688

2D gel databases

SWISS-2DPAGEⁱ	P00688

SWISS-2DPAGEⁱ

P00688

PTM databases

iPTMnetⁱ	P00688
PhosphoSitePlusⁱ	P00688

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000074268 Expressed in 10 organ(s), highest expression level in dorsal pancreas
Genevisibleⁱ	P00688 MM

Interactionⁱ

Subunit structureⁱ

Monomer.By similarity

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000137025

STRINGⁱ

10090.ENSMUSP00000137025

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P00688
SMRⁱ	P00688
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG2212 Eukaryota COG0366 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000002882
HOGENOMⁱ	HOG000253313
HOVERGENⁱ	HBG000061
InParanoidⁱ	P00688
KEGG Orthology (KO) More...KOⁱ	K01176
OMAⁱ	CNDVGIR
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G07V6
TreeFamⁱ	TF312850

Family and domain databases

Gene3Dⁱ	2.60.40.1180, 1 hit
InterProⁱ	View protein in InterPro IPR006048 A-amylase/branching_C IPR031319 A-amylase_C IPR006046 Alpha_amylase IPR006047 Glyco_hydro_13_cat_dom IPR013780 Glyco_hydro_b IPR017853 Glycoside_hydrolase_SF
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00128 Alpha-amylase, 1 hit PF02806 Alpha-amylase_C, 1 hit
PRINTSⁱ	PR00110 ALPHAAMYLASE
SMARTⁱ	View protein in SMART SM00642 Aamy, 1 hit SM00632 Aamy_C, 1 hit
SUPFAMⁱ	SSF51445 SSF51445, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P00688-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKFVLLLSLI GFCWAQYDPH TSDGRTAIVH LFEWRWVDIA KECERYLAPK 
        60         70         80         90        100
GFGGVQVSPP NENVVVHNPS RPWWERYQPI SYKICTRSGN EDEFRDMVTR 
       110        120        130        140        150
CNNVGVRIYV DAVINHMCGA GNPAGTSSTC GSYLNPNNRE FPAVPYSAWD 
       160        170        180        190        200
FNDNKCNGEI DNYNDAYQVR NCRLTGLLDL ALEKDYVRTK VADYMNHLID 
       210        220        230        240        250
IGVAGFRLDA AKHMWPGDIK AVLDKLHNLN TKWFSQGSRP FIFQEVIDLG 
       260        270        280        290        300
GEAIKGSEYF GNGRVTEFKY GAKLGTVIRK WNGEKMSYLK NWGEGWGLVP 
       310        320        330        340        350
SDRALVFVDN HDNQRGHGAG GSSILTFWDA RMYKMAVGFM LAHPYGFTRV 
       360        370        380        390        400
MSSYRWNRNF QNGKDQNDWI GPPNNNGVTK EVTINADTTC GNDWVCEHRW 
       410        420        430        440        450
RQIRNMVAFR NVVNGQPFSN WWDNNSNQVA FSRGNRGFIV FNNDDWALSA 
       460        470        480        490        500
TLQTGLPAGT YCDVISGDKV DGNCTGLRVN VGSDGKAHFS ISNSAEDPFI 

AIHADSKL

Length:508

Mass (Da):57,318

Last modified:July 27, 2011 - v2

Checksum:ⁱB204B03768132593

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	70	S → T in AAA37228 (PubMed:6176715).Curated	1
Sequence conflictⁱ	85	C → S in AAA37228 (PubMed:6176715).Curated	1
Sequence conflictⁱ	217	G → R in CAA24098 (PubMed:6157477).Curated	1
Sequence conflictⁱ	217	G → R in CAA26413 (PubMed:6098446).Curated	1
Sequence conflictⁱ	217	G → R in CAA26414 (PubMed:6098446).Curated	1
Sequence conflictⁱ	217	G → R in CAA26415 (PubMed:6098446).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	64	V → I in A1, B(C) and AMY-2.2Y.	1
Natural variantⁱ	66	V → I in AMY-2.2Y.	1
Natural variantⁱ	120	A → S in A1, B(A) and B(C).	1
Natural variantⁱ	161	D → S in A1, B(A) and B(C); requires 2 nucleotide substitutions.	1
Natural variantⁱ	174	L → V in A1 and B(C).	1
Natural variantⁱ	175	T → S in B(A).	1
Natural variantⁱ	254	I → V in A1 and B(C).	1
Natural variantⁱ	270 – 272	YGA → FGV in A1 and B(C).	3
Natural variantⁱ	298	L → M in A1, B(A) and B(C). Combined sources	1
Natural variantⁱ	322	S → A in B(A).	1
Natural variantⁱ	419	S → A in B1.	1
Natural variantⁱ	450	A → E in B(C).	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V00718 mRNA Translation: CAA24098.1 X02576 mRNA Translation: CAA26413.1 X02577 mRNA Translation: CAA26414.1 X02578 mRNA Translation: CAA26415.1 AK133526 mRNA Translation: BAE21706.1 BC094924 mRNA Translation: AAH94924.1 BC100579 mRNA Translation: AAI00580.1 J00357 Genomic DNA Translation: AAA37228.1 J00358 Genomic DNA Translation: AAA37229.1 J00361 Genomic DNA Translation: AAA37233.1 X02343 Genomic DNA Translation: CAA26202.1 M16540 Genomic DNA Translation: AAA37223.1 M15965 Genomic DNA Translation: AAA37226.1 M11895 mRNA Translation: AAA37224.1 M11896 mRNA Translation: AAA37227.1 M11891 Genomic DNA Translation: AAA37222.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS17775.1
PIRⁱ	A90995 ALMSP1 B90798 ALMSP B90995 ALMSPC C90995 ALMSPA I49493 I49494
NCBI Reference Sequences More...RefSeqⁱ	NP_001036176.1, NM_001042711.2 NP_001153622.1, NM_001160150.1 NP_001153623.1, NM_001160151.1 NP_001153624.1, NM_001160152.1
UniGeneⁱ	Mm.275426 Mm.443376 Mm.462977

Genome annotation databases

Ensemblⁱ	ENSMUST00000098667; ENSMUSP00000096264; ENSMUSG00000096569 ENSMUST00000098673; ENSMUSP00000096270; ENSMUSG00000074268 ENSMUST00000179314; ENSMUSP00000136894; ENSMUSG00000093931 ENSMUST00000179568; ENSMUSP00000137025; ENSMUSG00000096770
GeneIDⁱ	100043684 100043686 100043688 109959
KEGGⁱ	mmu:100043684 mmu:100043686 mmu:100043688 mmu:109959
UCSC genome browser More...UCSCⁱ	uc008rav.2 mouse

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P00688	Amylase 2a1 (Fragment)		MOUSE		245	UniRef100_P00688

Protein	Similar proteins		Species	Score	Length	Source
P00688	Pancreatic alpha-amylase		RAT		508	UniRef90_P00688
Amylase 2a1 (Fragment)		MOUSE		245
Alpha-amylase		CRIGR		514
Alpha-amylase		MESAU		508
UPI000334064B		OCTDE		508
+17

Protein	Similar proteins		Species	Score	Length	Source
P00688	Pancreatic alpha-amylase		RAT		508	UniRef50_P00688
Pancreatic alpha-amylase	)	PIG		511
Amylase 2a1 (Fragment)		MOUSE		245
Alpha-amylase		CRIGR		514
Alpha-amylase		MESAU		508
+450

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V00718 mRNA Translation: CAA24098.1 X02576 mRNA Translation: CAA26413.1 X02577 mRNA Translation: CAA26414.1 X02578 mRNA Translation: CAA26415.1 AK133526 mRNA Translation: BAE21706.1 BC094924 mRNA Translation: AAH94924.1 BC100579 mRNA Translation: AAI00580.1 J00357 Genomic DNA Translation: AAA37228.1 J00358 Genomic DNA Translation: AAA37229.1 J00361 Genomic DNA Translation: AAA37233.1 X02343 Genomic DNA Translation: CAA26202.1 M16540 Genomic DNA Translation: AAA37223.1 M15965 Genomic DNA Translation: AAA37226.1 M11895 mRNA Translation: AAA37224.1 M11896 mRNA Translation: AAA37227.1 M11891 Genomic DNA Translation: AAA37222.1
CCDSⁱ	CCDS17775.1
PIRⁱ	A90995 ALMSP1 B90798 ALMSP B90995 ALMSPC C90995 ALMSPA I49493 I49494
RefSeqⁱ	NP_001036176.1, NM_001042711.2 NP_001153622.1, NM_001160150.1 NP_001153623.1, NM_001160151.1 NP_001153624.1, NM_001160152.1
UniGeneⁱ	Mm.275426 Mm.443376 Mm.462977

3D structure databases

ProteinModelPortalⁱ	P00688
SMRⁱ	P00688
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000137025

STRINGⁱ

10090.ENSMUSP00000137025

Protein family/group databases

CAZyⁱ	GH13 Glycoside Hydrolase Family 13

CAZyⁱ

GH13 Glycoside Hydrolase Family 13

PTM databases

iPTMnetⁱ	P00688
PhosphoSitePlusⁱ	P00688

2D gel databases

SWISS-2DPAGEⁱ	P00688

SWISS-2DPAGEⁱ

P00688

Proteomic databases

MaxQBⁱ	P00688
PaxDbⁱ	P00688
PeptideAtlasⁱ	P00688
PRIDEⁱ	P00688

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	109959
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000098667; ENSMUSP00000096264; ENSMUSG00000096569 ENSMUST00000098673; ENSMUSP00000096270; ENSMUSG00000074268 ENSMUST00000179314; ENSMUSP00000136894; ENSMUSG00000093931 ENSMUST00000179568; ENSMUSP00000137025; ENSMUSG00000096770
GeneIDⁱ	100043684 100043686 100043688 109959
KEGGⁱ	mmu:100043684 mmu:100043686 mmu:100043688 mmu:109959
UCSCⁱ	uc008rav.2 mouse

Organism-specific databases

CTDⁱ	100043684 100043686 100043688 109959
MGIⁱ	MGI:88020 Amy2

Phylogenomic databases

eggNOGⁱ	KOG2212 Eukaryota COG0366 LUCA
GeneTreeⁱ	ENSGT00390000002882
HOGENOMⁱ	HOG000253313
HOVERGENⁱ	HBG000061
InParanoidⁱ	P00688
KOⁱ	K01176
OMAⁱ	CNDVGIR
OrthoDBⁱ	EOG091G07V6
TreeFamⁱ	TF312850

Enzyme and pathway databases

Reactomeⁱ	R-MMU-189085 Digestion of dietary carbohydrate

Reactomeⁱ

R-MMU-189085 Digestion of dietary carbohydrate

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P00688
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000074268 Expressed in 10 organ(s), highest expression level in dorsal pancreas
Genevisibleⁱ	P00688 MM

Family and domain databases

Gene3Dⁱ	2.60.40.1180, 1 hit
InterProⁱ	View protein in InterPro IPR006048 A-amylase/branching_C IPR031319 A-amylase_C IPR006046 Alpha_amylase IPR006047 Glyco_hydro_13_cat_dom IPR013780 Glyco_hydro_b IPR017853 Glycoside_hydrolase_SF
Pfamⁱ	View protein in Pfam PF00128 Alpha-amylase, 1 hit PF02806 Alpha-amylase_C, 1 hit
PRINTSⁱ	PR00110 ALPHAAMYLASE
SMARTⁱ	View protein in SMART SM00642 Aamy, 1 hit SM00632 Aamy_C, 1 hit
SUPFAMⁱ	SSF51445 SSF51445, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AMYP_MOUSE
Accessionⁱ	P00688Primary (citable) accession number: P00688 Secondary accession number(s): Q4VBW6 , Q61295, Q61296, Q64301
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	July 27, 2011
	Last modified:	November 7, 2018
	This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P00688 (AMYP_MOUSE)

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Pancreatic alpha-amylase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ