Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-amylase 1

Gene

Amy1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Hepatic and salivary alpha-amylases are encoded by the same gene; however, their mRNAs have different 5'-UTR sequences.

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115CalciumBy similarity1
Metal bindingi173Calcium; via carbonyl oxygenBy similarity1
Metal bindingi182CalciumBy similarity1
Binding sitei210ChlorideBy similarity1
Active sitei212NucleophileBy similarity1
Metal bindingi216Calcium; via carbonyl oxygenBy similarity1
Active sitei248Proton donorBy similarity1
Binding sitei313ChlorideBy similarity1
Sitei315Transition state stabilizerBy similarity1
Binding sitei352ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate catabolic process Source: MGI
  • response to bacterium Source: MGI

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 1 (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase 1
Salivary and hepatic alpha-amylase
Gene namesi
Name:Amy1
Synonyms:Amy-1-a, Amy1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:88019 Amy1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 151 PublicationAdd BLAST15
ChainiPRO_000000140316 – 511Alpha-amylase 1Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi43 ↔ 101By similarity
Disulfide bondi85 ↔ 130By similarity
Disulfide bondi156 ↔ 175By similarity
Disulfide bondi393 ↔ 399By similarity
Disulfide bondi465 ↔ 477By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP00687
PaxDbiP00687
PeptideAtlasiP00687
PRIDEiP00687

PTM databases

iPTMnetiP00687
PhosphoSitePlusiP00687

Expressioni

Tissue specificityi

Expressed in liver and saliva.

Gene expression databases

BgeeiENSMUSG00000074264 Expressed in 236 organ(s), highest expression level in lymph node
CleanExiMM_AMY1
ExpressionAtlasiP00687 baseline and differential
GenevisibleiP00687 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi198093, 2 interactors
IntActiP00687, 16 interactors
STRINGi10090.ENSMUSP00000070368

Structurei

3D structure databases

ProteinModelPortaliP00687
SMRiP00687
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP00687
KOiK01176
OMAiWKCQHAW
OrthoDBiEOG091G07V6
TreeFamiTF312850

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P00687-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKFFLLLSLI GFCWAQYDPH TQYGRTAIVH LFEWRWVDIA KECERYLAPN
60 70 80 90 100
GFAGVQVSPP NENIVVHSPS RPWWERYQPI SYKICSRSGN EDEFRDMVNR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGV GAQAGQSSTC GSYFNPNNRD FPGVPYSGFD
160 170 180 190 200
FNDGKCRTAS GGIENYQDAA QVRDCRLSGL LDLALEKDYV RTKVADYMNH
210 220 230 240 250
LIDIGVAGFR LDASKHMWPG DIKAILDKLH NLNTKWFSQG SRPFIFQEVI
260 270 280 290 300
DLGGEAVSSN EYFGNGRVTE FKYGAKLGKV MRKWDGEKMS YLKNWGEGWG
310 320 330 340 350
LMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF
360 370 380 390 400
TRVMSSYYWP RNFQNGKDVN DWVGPPNNNG KTKEVSINPD STCGNDWICE
410 420 430 440 450
HRWRQIRNMV AFRNVVNGQP FANWWDNDSN QVAFGRGNKG FIVFNNDDWA
460 470 480 490 500
LSETLQTGLP AGTYCDVISG DKVDGNCTGI KVYVGNDGKA HFSISNSAED
510
PFIAIHAESK I
Length:511
Mass (Da):57,644
Last modified:July 27, 2011 - v2
Checksum:iCC5399F4C23E1105
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YYQ5D3YYQ5_MOUSE
Alpha-amylase 1
Amy1
272Annotation score:
G3UXY7G3UXY7_MOUSE
Alpha-amylase 1
Amy1
131Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29V → I in AAA37221 (PubMed:6157477).Curated1
Sequence conflicti29V → I in CAA24097 (PubMed:6157477).Curated1
Sequence conflicti29V → I in CAA24099 (PubMed:6162108).Curated1
Sequence conflicti29V → I in CAA24100 (PubMed:6162108).Curated1
Sequence conflicti29V → I in AAA37219 (PubMed:6162570).Curated1
Sequence conflicti229L → Q in CAA24099 (PubMed:6162108).Curated1
Sequence conflicti229L → Q in CAA24100 (PubMed:6162108).Curated1
Sequence conflicti441F → L in AAA37221 (PubMed:6157477).Curated1
Sequence conflicti441F → L in CAA24097 (PubMed:6157477).Curated1
Sequence conflicti441F → L in CAA24099 (PubMed:6162108).Curated1
Sequence conflicti441F → L in CAA24100 (PubMed:6162108).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00356 mRNA Translation: AAA37221.1
V00717 mRNA Translation: CAA24097.1
V00719 mRNA Translation: CAA24099.1
V00720 mRNA Translation: CAA24100.1
AK029642 mRNA Translation: BAC26543.1
CH466532 Genomic DNA Translation: EDL12410.1
CH466532 Genomic DNA Translation: EDL12411.1
CH466532 Genomic DNA Translation: EDL12412.1
BC009121 mRNA Translation: AAH09121.1
J00353 Genomic DNA Translation: AAA37219.1
J00354 Genomic DNA Translation: AAA37220.1
CCDSiCCDS17776.1
PIRiA90798 ALMSS
RefSeqiNP_001103975.1, NM_001110505.1
NP_031472.2, NM_007446.2
UniGeneiMm.439727

Genome annotation databases

EnsembliENSMUST00000067980; ENSMUSP00000070368; ENSMUSG00000074264
ENSMUST00000106540; ENSMUSP00000102150; ENSMUSG00000074264
GeneIDi11722
KEGGimmu:11722
UCSCiuc008rba.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00356 mRNA Translation: AAA37221.1
V00717 mRNA Translation: CAA24097.1
V00719 mRNA Translation: CAA24099.1
V00720 mRNA Translation: CAA24100.1
AK029642 mRNA Translation: BAC26543.1
CH466532 Genomic DNA Translation: EDL12410.1
CH466532 Genomic DNA Translation: EDL12411.1
CH466532 Genomic DNA Translation: EDL12412.1
BC009121 mRNA Translation: AAH09121.1
J00353 Genomic DNA Translation: AAA37219.1
J00354 Genomic DNA Translation: AAA37220.1
CCDSiCCDS17776.1
PIRiA90798 ALMSS
RefSeqiNP_001103975.1, NM_001110505.1
NP_031472.2, NM_007446.2
UniGeneiMm.439727

3D structure databases

ProteinModelPortaliP00687
SMRiP00687
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198093, 2 interactors
IntActiP00687, 16 interactors
STRINGi10090.ENSMUSP00000070368

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

PTM databases

iPTMnetiP00687
PhosphoSitePlusiP00687

Proteomic databases

MaxQBiP00687
PaxDbiP00687
PeptideAtlasiP00687
PRIDEiP00687

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067980; ENSMUSP00000070368; ENSMUSG00000074264
ENSMUST00000106540; ENSMUSP00000102150; ENSMUSG00000074264
GeneIDi11722
KEGGimmu:11722
UCSCiuc008rba.2 mouse

Organism-specific databases

CTDi11722
MGIiMGI:88019 Amy1

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP00687
KOiK01176
OMAiWKCQHAW
OrthoDBiEOG091G07V6
TreeFamiTF312850

Enzyme and pathway databases

ReactomeiR-MMU-189085 Digestion of dietary carbohydrate

Miscellaneous databases

ChiTaRSiAmy1 mouse
PROiPR:P00687
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074264 Expressed in 236 organ(s), highest expression level in lymph node
CleanExiMM_AMY1
ExpressionAtlasiP00687 baseline and differential
GenevisibleiP00687 MM

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY1_MOUSE
AccessioniPrimary (citable) accession number: P00687
Secondary accession number(s): Q921Y7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again