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Protein

Ribonuclease pancreatic

Gene

Rnase1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32SubstrateBy similarity1
Binding sitei35SubstrateBy similarity1
Active sitei37Proton acceptorBy similarity1
Binding sitei91SubstrateBy similarity1
Active sitei144Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • response to bacterium Source: MGI
  • RNA phosphodiester bond hydrolysis Source: MGI

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease pancreatic (EC:3.1.27.5)
Alternative name(s):
RNase 1
RNase A
Gene namesi
Name:Rnase1
Synonyms:Rib-1, Rib1, Rns1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97919 Rnase1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000003092926 – 149Ribonuclease pancreaticAdd BLAST124

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 109By similarity
Disulfide bondi65 ↔ 120By similarity
Disulfide bondi83 ↔ 135By similarity
Disulfide bondi90 ↔ 97By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP00683
PaxDbiP00683
PeptideAtlasiP00683
PRIDEiP00683

PTM databases

PhosphoSitePlusiP00683

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

CleanExiMM_RNASE1

Interactioni

Subunit structurei

Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079025

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00683
SMRiP00683
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni66 – 70Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IYEN Eukaryota
ENOG41113F1 LUCA
HOGENOMiHOG000276883
HOVERGENiHBG008396
InParanoidiP00683
PhylomeDBiP00683

Family and domain databases

Gene3Di3.10.130.10, 1 hit
InterProiView protein in InterPro
IPR001427 RNaseA
IPR036816 RNaseA-like_dom_sf
IPR023411 RNaseA_AS
IPR023412 RNaseA_domain
PANTHERiPTHR11437 PTHR11437, 1 hit
PfamiView protein in Pfam
PF00074 RnaseA, 1 hit
PRINTSiPR00794 RIBONUCLEASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000535 RNaseA, 1 hit
SMARTiView protein in SMART
SM00092 RNAse_Pc, 1 hit
SUPFAMiSSF54076 SSF54076, 1 hit
PROSITEiView protein in PROSITE
PS00127 RNASE_PANCREATIC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P00683-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLEKSLILF PLFFLLLGWV QPSLGRESAA QKFQRQHMDP DGSSINSPTY
60 70 80 90 100
CNQMMKRRDM TNGSCKPVNT FVHEPLADVQ AVCSQENVTC KNRKSNCYKS
110 120 130 140
SSALHITDCH LKGNSKYPNC DYKTTQYQKH IIVACEGNPY VPVHFDATV
Length:149
Mass (Da):16,820
Last modified:May 1, 1991 - v2
Checksum:i2FC1E0A50D245162
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8C6G3Q8C6G3_MOUSE
Ribonuclease pancreatic
Rnase1
149Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27814 mRNA Translation: AAA40060.1
X60103 Genomic DNA Translation: CAA42697.1
CCDSiCCDS27038.1
PIRiA34090 NRMS
UniGeneiMm.235538

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27814 mRNA Translation: AAA40060.1
X60103 Genomic DNA Translation: CAA42697.1
CCDSiCCDS27038.1
PIRiA34090 NRMS
UniGeneiMm.235538

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TSRX-ray2.20A/B/C/D26-149[»]
ProteinModelPortaliP00683
SMRiP00683
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000079025

PTM databases

PhosphoSitePlusiP00683

Proteomic databases

MaxQBiP00683
PaxDbiP00683
PeptideAtlasiP00683
PRIDEiP00683

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:97919 Rnase1

Phylogenomic databases

eggNOGiENOG410IYEN Eukaryota
ENOG41113F1 LUCA
HOGENOMiHOG000276883
HOVERGENiHBG008396
InParanoidiP00683
PhylomeDBiP00683

Miscellaneous databases

ChiTaRSiRnase1 mouse
PROiPR:P00683
SOURCEiSearch...

Gene expression databases

CleanExiMM_RNASE1

Family and domain databases

Gene3Di3.10.130.10, 1 hit
InterProiView protein in InterPro
IPR001427 RNaseA
IPR036816 RNaseA-like_dom_sf
IPR023411 RNaseA_AS
IPR023412 RNaseA_domain
PANTHERiPTHR11437 PTHR11437, 1 hit
PfamiView protein in Pfam
PF00074 RnaseA, 1 hit
PRINTSiPR00794 RIBONUCLEASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000535 RNaseA, 1 hit
SMARTiView protein in SMART
SM00092 RNAse_Pc, 1 hit
SUPFAMiSSF54076 SSF54076, 1 hit
PROSITEiView protein in PROSITE
PS00127 RNASE_PANCREATIC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNAS1_MOUSE
AccessioniPrimary (citable) accession number: P00683
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 1, 1991
Last modified: November 7, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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