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Protein

Ribonuclease alpha-sarcin

Gene

sar

Organism
Aspergillus giganteus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha-sarcin is specific for purines in both single- and double-stranded RNA. Its toxic action on eukaryotic cells is the result of cleavage of a single phosphodiester bond in the 60S subunit of ribosomes.

Catalytic activityi

Hydrolysis of the phosphodiester linkage between guanosine and adenosine residues at one specific position in 28S rRNA from rat ribosomes.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei771
Active sitei123Proton acceptor1
Active sitei164Proton donor1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease, Protein synthesis inhibitor

Enzyme and pathway databases

BRENDAi3.1.27.10 510

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease alpha-sarcin (EC:3.1.27.10)
Alternative name(s):
rRNA endonuclease
Gene namesi
Name:sar
OrganismiAspergillus giganteus
Taxonomic identifieri5060 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000003083628 – 177Ribonuclease alpha-sarcinAdd BLAST150

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 175
Disulfide bondi103 ↔ 159

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1177
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00655
SMRiP00655
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00655

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease U2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK01171

Family and domain databases

InterProiView protein in InterPro
IPR004025 Fun_ribotoxin
IPR000026 Gua-sp_ribonuclease_N1/T1/U2
IPR016191 Ribonuclease/ribotoxin
PIRSFiPIRSF037430 RNase_U2, 1 hit
PRINTSiPR01704 FUNRIBOTOXIN
SUPFAMiSSF53933 SSF53933, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00655-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVAIKNLVLV ALTAVTALAV PSPLEARAVT WTCLNDQKNP KTNKYETKRL
60 70 80 90 100
LYNQNKAESN SHHAPLSDGK TGSSYPHWFT NGYDGDGKLP KGRTPIKFGK
110 120 130 140 150
SDCDRPPKHS KDGNGKTDHY LLEFPTFPDG HDYKFDSKKP KENPGPARVI
160 170
YTYPNKVFCG IIAHTKENQG ELKLCSH
Length:177
Mass (Da):19,724
Last modified:March 1, 1992 - v2
Checksum:i6C711D9482DC9DD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13704 mRNA Translation: BAA02863.1
X60770 Genomic DNA Translation: CAA43180.1
PIRiJU0138 NRASSG

Genome annotation databases

KEGGiag:BAA02863

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13704 mRNA Translation: BAA02863.1
X60770 Genomic DNA Translation: CAA43180.1
PIRiJU0138 NRASSG

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DE3NMR-A28-177[»]
1R4YNMR-A28-177[»]
ProteinModelPortaliP00655
SMRiP00655
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAA02863

Phylogenomic databases

KOiK01171

Enzyme and pathway databases

BRENDAi3.1.27.10 510

Miscellaneous databases

EvolutionaryTraceiP00655

Family and domain databases

InterProiView protein in InterPro
IPR004025 Fun_ribotoxin
IPR000026 Gua-sp_ribonuclease_N1/T1/U2
IPR016191 Ribonuclease/ribotoxin
PIRSFiPIRSF037430 RNase_U2, 1 hit
PRINTSiPR01704 FUNRIBOTOXIN
SUPFAMiSSF53933 SSF53933, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNAS_ASPGI
AccessioniPrimary (citable) accession number: P00655
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 1, 1992
Last modified: May 10, 2017
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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