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Protein

Guanyl-specific ribonuclease T1

Gene

rntA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei661 Publication1
Active sitei84Proton acceptor1 Publication1
Active sitei118Proton donor1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.27.3 522
SABIO-RKiP00651

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease T1 (EC:3.1.27.3)
Short name:
RNase T1
Gene namesi
Name:rntA
ORF Names:AO090011000118
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 7

Subcellular locationi

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075043

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000003083327 – 130Guanyl-specific ribonuclease T1Add BLAST104

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 361 Publication
Disulfide bondi32 ↔ 1291 Publication

Keywords - PTMi

Disulfide bond

PTM databases

CarbonylDBiP00651

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi5062.CADAORAP00004829

Chemistry databases

BindingDBiP00651

Structurei

Secondary structure

1130
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00651
SMRiP00651
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00651

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000217295
OMAiYHDYEGF
OrthoDBiEOG092C58FH

Family and domain databases

InterProiView protein in InterPro
IPR000026 Gua-sp_ribonuclease_N1/T1/U2
IPR016191 Ribonuclease/ribotoxin
PfamiView protein in Pfam
PF00545 Ribonuclease, 1 hit
PIRSFiPIRSF037430 RNase_U2, 1 hit
SUPFAMiSSF53933 SSF53933, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00651-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMYSKLLTLT TLLLPTALAL PSLVERACDY TCGSNCYSSS DVSTAQAAGY
60 70 80 90 100
QLHEDGETVG SNSYPHKYNN YEGFDFSVSS PYYEWPILSS GDVYSGGSPG
110 120 130
ADRVVFNENN QLAGVITHTG ASGNNFVECT
Length:130
Mass (Da):13,960
Last modified:November 1, 1995 - v2
Checksum:i3F49EF41E85E230A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28341 Genomic DNA Translation: BAA05707.1
D49428 mRNA Translation: BAA08407.1
AP007171 Genomic DNA Translation: BAE64671.1
PIRiJC4325 NRAST1
RefSeqiXP_001825804.1, XM_001825752.2

Genome annotation databases

EnsemblFungiiBAE64671; BAE64671; AO090011000118
GeneIDi5997907
KEGGiaor:AO090011000118

Similar proteinsi

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28341 Genomic DNA Translation: BAA05707.1
D49428 mRNA Translation: BAA08407.1
AP007171 Genomic DNA Translation: BAE64671.1
PIRiJC4325 NRAST1
RefSeqiXP_001825804.1, XM_001825752.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B2MX-ray2.00A/B27-130[»]
1BIRX-ray1.80A/B27-130[»]
1BU4X-ray1.90A27-130[»]
1BVIX-ray1.90A/B/C/D27-130[»]
1CH0X-ray2.30A/B/C27-130[»]
1DETX-ray1.80A27-130[»]
1FYSX-ray2.00A27-130[»]
1FZUX-ray1.80A27-130[»]
1G02X-ray1.86A27-130[»]
1GSPX-ray2.20A27-130[»]
1HYFX-ray1.70A27-130[»]
1HZ1X-ray1.80A27-130[»]
1I0VX-ray1.23A27-130[»]
1I0XX-ray1.65A/B/C/D27-130[»]
1I2EX-ray1.80A27-130[»]
1I2FX-ray1.95A27-130[»]
1I2GX-ray1.85A27-130[»]
1I3FX-ray2.35A27-130[»]
1I3IX-ray1.76A27-130[»]
1IYYNMR-A27-130[»]
1LOVX-ray1.55A27-130[»]
1LOWX-ray1.90A27-130[»]
1LOYX-ray1.55A27-130[»]
1LRAX-ray1.90A27-130[»]
1Q9EX-ray1.70A/B/C27-130[»]
1RGAX-ray1.70A27-130[»]
1RGCX-ray2.00A/B27-130[»]
1RGKX-ray1.87A27-130[»]
1RGLX-ray2.00A27-130[»]
1RHLX-ray1.95A27-130[»]
1RLSX-ray1.90A27-130[»]
1RN1X-ray1.84A/B/C27-130[»]
1RN4X-ray1.80A27-130[»]
1RNTX-ray1.90A27-130[»]
1TRPX-ray2.40A/B27-130[»]
1TRQX-ray2.30A/B27-130[»]
1TTOX-ray2.10A/B/C27-130[»]
1YGWNMR-A27-130[»]
2AADX-ray2.00A/B27-130[»]
2AAEX-ray1.80A27-130[»]
2BIRX-ray2.30A27-130[»]
2BU4X-ray1.95A27-130[»]
2GSPX-ray1.80A27-130[»]
2HOHX-ray1.90A/B/C/D27-130[»]
2RNTX-ray1.80A27-130[»]
3BIRX-ray1.80A27-130[»]
3BU4X-ray1.77A27-130[»]
3GSPX-ray1.90A27-130[»]
3HOHX-ray1.95A/B/C/D27-130[»]
3RNTX-ray1.80A27-130[»]
3SYUX-ray1.95A27-130[»]
3URPX-ray3.19A27-130[»]
4BIRX-ray1.70A27-130[»]
4BU4X-ray1.80A27-130[»]
4GSPX-ray1.65A27-130[»]
4HOHX-ray2.05A/B/C/D27-130[»]
4ODKX-ray1.40B/C59-73[»]
4RNTX-ray2.20A27-130[»]
5BIRX-ray2.00A/B27-130[»]
5BU4X-ray1.77A27-130[»]
5GSPX-ray1.80A27-130[»]
5HOHX-ray2.00A/B/C/D27-130[»]
5RNTX-ray3.20A27-130[»]
6GSPX-ray2.20A27-130[»]
6RNTX-ray1.80A27-130[»]
7GSPX-ray2.00A/B27-130[»]
7RNTX-ray1.90A27-130[»]
8RNTX-ray1.80A27-130[»]
9RNTX-ray1.50A27-130[»]
ProteinModelPortaliP00651
SMRiP00651
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5062.CADAORAP00004829

Chemistry databases

BindingDBiP00651
ChEMBLiCHEMBL1075043

PTM databases

CarbonylDBiP00651

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAE64671; BAE64671; AO090011000118
GeneIDi5997907
KEGGiaor:AO090011000118

Phylogenomic databases

HOGENOMiHOG000217295
OMAiYHDYEGF
OrthoDBiEOG092C58FH

Enzyme and pathway databases

BRENDAi3.1.27.3 522
SABIO-RKiP00651

Miscellaneous databases

EvolutionaryTraceiP00651
PROiPR:P00651

Family and domain databases

InterProiView protein in InterPro
IPR000026 Gua-sp_ribonuclease_N1/T1/U2
IPR016191 Ribonuclease/ribotoxin
PfamiView protein in Pfam
PF00545 Ribonuclease, 1 hit
PIRSFiPIRSF037430 RNase_U2, 1 hit
SUPFAMiSSF53933 SSF53933, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNT1_ASPOR
AccessioniPrimary (citable) accession number: P00651
Secondary accession number(s): Q2U194
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: February 28, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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