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Protein

Ribonuclease

Gene
N/A
Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei120Proton acceptor1
Active sitei149Proton donor1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.27.3 630

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease (EC:3.1.27.-)
Alternative name(s):
Barnase
RNase Ba
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus amyloliquefaciens group

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

Introduced by genetic manipulation and expressed in male sterile maize and rape by Plant Genetic Systems and in radicchio rosso by Bejo Zaden. Barnase expressed in transgenic plants will specifically destroy the tissue(s) where it is expressed. After cell transformation, protein expression and plant regeneration, the active nuclease destroys the pollen producing organs thus rendering the plant sterile.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi149H → Q: Loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Add BLAST34
PropeptideiPRO_000003082835 – 471 PublicationAdd BLAST13
ChainiPRO_000003082948 – 157Ribonuclease1 PublicationAdd BLAST110

Interactioni

Protein-protein interaction databases

DIPiDIP-544N
IntActiP00648, 1 interactor

Structurei

Secondary structure

1157
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi54 – 64Combined sources11
Helixi74 – 79Combined sources6
Helixi84 – 86Combined sources3
Helixi89 – 92Combined sources4
Beta strandi97 – 103Combined sources7
Beta strandi118 – 122Combined sources5
Beta strandi127 – 129Combined sources3
Beta strandi134 – 138Combined sources5
Beta strandi143 – 148Combined sources6
Beta strandi154 – 157Combined sources4

3D structure databases

ProteinModelPortaliP00648
SMRiP00648
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00648

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105GGE Bacteria
ENOG4111TSC LUCA

Family and domain databases

CDDicd00933 barnase, 1 hit
InterProiView protein in InterPro
IPR001887 Barnase
IPR000026 Gua-sp_ribonuclease_N1/T1/U2
IPR016191 Ribonuclease/ribotoxin
PfamiView protein in Pfam
PF00545 Ribonuclease, 1 hit
PIRSFiPIRSF001013 Barnase, 1 hit
PRINTSiPR00117 BARNASE
SUPFAMiSSF53933 SSF53933, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKMEGIALK KRLSWISVCL LVLVSAAGML FSTAAKTETS SHKAHTEAQV
60 70 80 90 100
INTFDGVADY LQTYHKLPDN YITKSEAQAL GWVASKGNLA DVAPGKSIGG
110 120 130 140 150
DIFSNREGKL PGKSGRTWRE ADINYTSGFR NSDRILYSSD WLIYKTTDHY

QTFTKIR
Length:157
Mass (Da):17,473
Last modified:July 1, 1989 - v2
Checksum:i42B6669F9B0D7FBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14442 Genomic DNA Translation: AAA86441.1
X12871 Genomic DNA Translation: CAA31365.1
PIRiA24038 NRBSN

Similar proteinsi

Entry informationi

Entry nameiRNBR_BACAM
AccessioniPrimary (citable) accession number: P00648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: August 30, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Genetically modified food

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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