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Protein

Thermonuclease

Gene

nuc

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.PROSITE-ProRule annotation

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi103Calcium1
Active sitei1171 Publication1
Metal bindingi122Calcium1
Metal bindingi123Calcium; via carbonyl oxygen1
Active sitei1251 Publication1
Active sitei1691 Publication1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.31.1 3352

Names & Taxonomyi

Protein namesi
Recommended name:
Thermonuclease (EC:3.1.31.1)
Short name:
TNase
Alternative name(s):
Micrococcal nuclease
Staphylococcal nuclease
Cleaved into the following 2 chains:
Gene namesi
Name:nuc
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
PropeptideiPRO_000004522627 – 631 PublicationAdd BLAST37
ChainiPRO_000003438964 – 231Nuclease BAdd BLAST168
PropeptideiPRO_000003439064 – 821 PublicationAdd BLAST19
ChainiPRO_000003439183 – 231Nuclease AAdd BLAST149

Keywords - PTMi

Zymogen

Structurei

Secondary structure

1231
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00644
SMRiP00644
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00644

Family & Domainsi

Sequence similaritiesi

Belongs to the thermonuclease family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107Y9E Bacteria
COG1525 LUCA

Family and domain databases

Gene3Di2.40.50.90, 1 hit
InterProiView protein in InterPro
IPR035437 SNase_OB-fold_sf
IPR016071 Staphylococal_nuclease_OB-fold
IPR002071 Thermonucl_AS
PfamiView protein in Pfam
PF00565 SNase, 1 hit
SMARTiView protein in SMART
SM00318 SNc, 1 hit
PROSITEiView protein in PROSITE
PS01123 TNASE_1, 1 hit
PS01284 TNASE_2, 1 hit
PS50830 TNASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00644-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLVMTEYLLS AGICMAIVSI LLIGMAISNV SKGQYAKRFF FFATSCLVLT
60 70 80 90 100
LVVVSSLSSS ANASQTDNGV NRSGSEDPTV YSATSTKKLH KEPATLIKAI
110 120 130 140 150
DGDTVKLMYK GQPMTFRLLL VDTPETKHPK KGVEKYGPEA SAFTKKMVEN
160 170 180 190 200
AKKIEVEFDK GQRTDKYGRG LAYIYADGKM VNEALVRQGL AKVAYVYKPN
210 220 230
NTHEQHLRKS EAQAKKEKLN IWSEDNADSG Q
Length:231
Mass (Da):25,471
Last modified:July 21, 1986 - v1
Checksum:iAD5558AA28E855D7
GO

Sequence cautioni

The sequence AAA26659 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01281 mRNA Translation: CAA24594.1
M17123 Genomic DNA Translation: AAA26659.1 Different initiation.
PIRiA00790 NCSAF

Similar proteinsi

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01281 mRNA Translation: CAA24594.1
M17123 Genomic DNA Translation: AAA26659.1 Different initiation.
PIRiA00790 NCSAF

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A2TX-ray1.96A83-231[»]
1A2UX-ray2.00A83-231[»]
1A3TX-ray2.10A83-231[»]
1A3UX-ray2.05A83-231[»]
1A3VX-ray2.20A83-231[»]
1AEXX-ray2.10A83-231[»]
1ENAX-ray2.15A89-223[»]
1ENCX-ray1.95A83-231[»]
1EQVX-ray1.94A88-223[»]
1EY0X-ray1.60A83-231[»]
1EY4X-ray1.60A83-231[»]
1EY5X-ray1.70A83-231[»]
1EY6X-ray1.75A83-231[»]
1EY7X-ray1.88A83-231[»]
1EY8X-ray1.75A83-231[»]
1EY9X-ray1.72A83-231[»]
1EYAX-ray2.00A83-231[»]
1EYCX-ray1.85A83-231[»]
1EYDX-ray1.70A83-231[»]
1EZ6X-ray1.90A83-231[»]
1EZ8X-ray1.85A83-231[»]
1F2MX-ray2.00A83-231[»]
1F2YX-ray2.00A83-231[»]
1F2ZX-ray1.80A83-231[»]
1IHZX-ray1.65A83-231[»]
1II3X-ray1.72A83-231[»]
1JOKNMR-A83-231[»]
1JOONMR-A83-231[»]
1JOQNMR-A83-231[»]
1JORNMR-A83-231[»]
1KAAX-ray1.90A88-223[»]
1KABX-ray1.80A88-223[»]
1KDAX-ray1.90A83-231[»]
1KDBX-ray1.90A83-231[»]
1KDCX-ray2.00A83-231[»]
1NSNX-ray2.80S83-231[»]
1NUCX-ray1.90A83-231[»]
1RKNNMR-A83-192[»]
1SNCX-ray1.65A83-231[»]
1SNDX-ray1.84A/B83-231[»]
1SNMX-ray1.74A83-231[»]
1SNOX-ray1.70A83-231[»]
1SNPX-ray1.95A83-231[»]
1SNQX-ray1.70A83-231[»]
1STAX-ray1.70A83-231[»]
1STBX-ray2.00A83-230[»]
1STGX-ray1.70A83-231[»]
1STHX-ray1.85A83-231[»]
1STNX-ray1.70A83-231[»]
1STYX-ray1.67A83-231[»]
1SYBX-ray1.80A83-231[»]
1SYCX-ray1.80A83-231[»]
1SYDX-ray1.70A83-231[»]
1SYEX-ray1.80A83-231[»]
1SYFX-ray1.80A83-231[»]
1SYGX-ray1.90A83-231[»]
1TQOX-ray2.00A83-226[»]
1TR5X-ray2.10A83-226[»]
1TT2X-ray1.85A83-226[»]
1U9RX-ray2.10A83-231[»]
2ENBX-ray2.05A89-223[»]
2EXZX-ray1.90A83-231[»]
2EY1X-ray1.85A83-231[»]
2EY2X-ray1.70A83-231[»]
2EY5X-ray2.00A83-231[»]
2EY6X-ray1.65A83-231[»]
2EYFX-ray1.80A83-231[»]
2EYHX-ray1.90A83-231[»]
2EYJX-ray1.85A83-231[»]
2EYLX-ray1.70A83-231[»]
2EYMX-ray1.80A83-231[»]
2EYOX-ray1.70A83-231[»]
2EYPX-ray1.80A83-231[»]
2F0DX-ray2.50A83-231[»]
2F0EX-ray1.95A83-231[»]
2F0FX-ray2.15A83-231[»]
2F0GX-ray1.90A83-231[»]
2F0HX-ray2.15A83-231[»]
2F0IX-ray1.80A83-231[»]
2F0JX-ray1.80A83-231[»]
2F0KX-ray1.90A83-231[»]
2F0LX-ray1.90A83-231[»]
2F0MX-ray2.00A83-231[»]
2F0NX-ray1.70A83-231[»]
2F0OX-ray1.90A83-231[»]
2F0PX-ray2.05A83-231[»]
2F0QX-ray1.95A83-231[»]
2F0SX-ray1.75A83-231[»]
2F0TX-ray1.70A83-231[»]
2F0UX-ray1.83A83-231[»]
2F0VX-ray2.00A83-231[»]
2F0WX-ray2.10A83-231[»]
2F3VNMR-A83-192[»]
2F3WNMR-A83-192[»]
2FXYNMR-A137-154[»]
2FXZNMR-A179-191[»]
2GSIX-ray2.81W/X/Y/Z126-136[»]
2KHSNMR-A83-203[»]
2NUCX-ray2.00A83-231[»]
2OEOX-ray2.00A83-226[»]
2OF1X-ray1.92A83-226[»]
2PQENMR-A83-231[»]
2QDBX-ray2.20A83-231[»]
2RBMX-ray1.90A83-231[»]
2RDFX-ray2.01A83-231[»]
2RKSX-ray2.01A83-231[»]
2SNMX-ray1.97A83-231[»]
2SNSX-ray1.50A83-231[»]
2SOBNMR-A83-185[»]
3BDCX-ray1.80A83-231[»]
3C1EX-ray1.90A83-231[»]
3C1FX-ray2.00A83-231[»]
3D4DX-ray2.10A/B83-231[»]
3D6CX-ray2.00A83-231[»]
3DHQX-ray2.15A83-231[»]
3DMUX-ray1.80A83-231[»]
3E5SX-ray2.00A/B83-231[»]
3EJIX-ray1.90A83-231[»]
3EROX-ray1.85A83-231[»]
3ERQX-ray2.10A83-231[»]
3EVQX-ray2.00A83-231[»]
3H6MX-ray1.70A83-231[»]
3HEJX-ray1.80A/B/C/D83-231[»]
3HZXX-ray2.00A83-231[»]
3ITPX-ray1.75A83-231[»]
3LX0X-ray1.50A83-231[»]
3MEHX-ray1.50A83-231[»]
3MHBX-ray1.55A83-231[»]
3MXPX-ray1.61A83-231[»]
3MZ5X-ray1.58A83-231[»]
3NHHX-ray2.00A83-231[»]
3NK9X-ray1.65A83-231[»]
3NP8X-ray1.70A83-231[»]
3NQTX-ray1.90A83-231[»]
3NUCX-ray1.90A83-231[»]
3NXWX-ray1.65A83-231[»]
3OSOX-ray1.60A83-231[»]
3OWFX-ray1.85A83-231[»]
3P1HX-ray1.79A83-231[»]
3P75X-ray1.90A89-223[»]
3PMFX-ray1.60A83-231[»]
3QB3X-ray1.63A83-231[»]
3RUZX-ray1.58A83-231[»]
3S9WX-ray1.90A83-231[»]
3SHLX-ray1.48A83-231[»]
3SK4X-ray1.85A83-231[»]
3SK5X-ray1.70A83-231[»]
3SK6X-ray1.80A83-231[»]
3SK8X-ray1.90A83-231[»]
3SR1X-ray1.45A83-231[»]
3SXHX-ray1.40A83-231[»]
3T13X-ray1.80A/B83-231[»]
3T16X-ray1.80A83-231[»]
3TMEX-ray1.40A83-231[»]
3TP5X-ray1.80A83-231[»]
3TP6X-ray1.80A83-231[»]
3TP7X-ray1.90A83-231[»]
3TP8X-ray1.60A83-231[»]
3U9OX-ray1.95A83-231[»]
3V2TX-ray1.80A83-231[»]
3VA5X-ray1.55A83-231[»]
4DF7X-ray1.50A83-231[»]
4DFAX-ray1.40A83-231[»]
4DGZX-ray1.47A83-231[»]
4DU9X-ray1.63A83-231[»]
4E6IX-ray2.00A/B83-231[»]
4EQNX-ray1.80A83-231[»]
4EQOX-ray1.70A83-231[»]
4EQPX-ray1.35A83-231[»]
4EVOX-ray1.95A83-231[»]
4F7XX-ray1.70A83-231[»]
4F8MX-ray1.60A83-231[»]
4G57X-ray1.81A89-223[»]
4H7BX-ray1.60A83-231[»]
4HMIX-ray1.75A83-231[»]
4HMJX-ray1.35A83-231[»]
4HTHX-ray1.75A83-231[»]
4I65X-ray1.61A88-223[»]
4IALX-ray1.60A83-231[»]
4ID6X-ray1.90A83-231[»]
4IUNX-ray1.60A83-231[»]
4IZ8X-ray1.70A83-231[»]
4J1MX-ray1.65A83-231[»]
4J6HX-ray1.60A83-231[»]
4K14X-ray1.60A88-223[»]
4K2KX-ray1.65A83-231[»]
4K2LX-ray1.55A83-231[»]
4K5VX-ray1.75A83-231[»]
4K5WX-ray1.65A83-231[»]
4K5XX-ray1.65A83-231[»]
4K6DX-ray1.65A83-231[»]
4K8IX-ray2.10A89-223[»]
4K8JX-ray2.00A89-223[»]
4KD3X-ray1.75A83-231[»]
4KD4X-ray1.55A83-231[»]
4KHVX-ray1.60A83-231[»]
4KJNX-ray1.55A83-231[»]
4KJOX-ray1.90A83-231[»]
4KTAX-ray1.95A83-231[»]
4KV6X-ray1.55A83-231[»]
4KY5X-ray1.40A83-231[»]
4KY6X-ray1.70A83-231[»]
4KY7X-ray1.55A83-231[»]
4LAAX-ray1.58A83-231[»]
4ME5X-ray1.80A83-231[»]
4MIUX-ray1.67A83-231[»]
4N9PX-ray1.40A83-231[»]
4N9TX-ray1.60A83-231[»]
4NDXX-ray1.50A83-231[»]
4NKLX-ray1.60A83-231[»]
4NMZX-ray1.70A83-231[»]
4NP5X-ray1.50A83-231[»]
4ODGX-ray1.73A83-231[»]
4OL7X-ray1.67A/B83-231[»]
4PMBX-ray1.80A83-231[»]
4PMCX-ray1.65A83-231[»]
4PNYX-ray1.68A83-231[»]
4QB4X-ray1.60A88-223[»]
4QF4X-ray1.80A/B83-231[»]
4R8NX-ray1.65A83-223[»]
4RKBX-ray1.88A83-231[»]
4RKLX-ray1.66A83-231[»]
4S3SX-ray1.64A83-231[»]
4TRDX-ray1.60A83-231[»]
4WORX-ray1.60A83-231[»]
4YIJX-ray1.64A/B83-231[»]
4ZQ3X-ray1.90A83-231[»]
4ZUIX-ray1.70A83-231[»]
4ZUJX-ray1.72A83-231[»]
5C3WX-ray1.72A83-231[»]
5C3XX-ray1.95A83-231[»]
5C4HX-ray1.80A83-231[»]
5C4ZX-ray1.62A83-231[»]
5C5LX-ray1.75A83-231[»]
5C5MX-ray1.70A83-231[»]
5C6AX-ray1.85A83-231[»]
5CC4X-ray1.90A83-231[»]
5CGKX-ray1.85A83-231[»]
5CR3X-ray1.80A83-231[»]
5CV4X-ray1.80A83-231[»]
5CV5X-ray1.80A/B83-231[»]
5CV6X-ray1.95A83-231[»]
5CV7X-ray1.65A83-231[»]
5CV8X-ray1.58A83-231[»]
5CV9X-ray1.62A83-231[»]
5DEHX-ray1.70A83-231[»]
5E1FX-ray1.90A83-231[»]
5E3FX-ray1.75A83-231[»]
5EGTX-ray1.85A83-231[»]
5EKKX-ray2.10A83-231[»]
5EKLX-ray2.10A83-231[»]
5F2DX-ray1.70A83-231[»]
5HGTX-ray1.75A83-231[»]
5HURX-ray1.50A83-231[»]
5I6WX-ray1.90A83-231[»]
5I6YX-ray1.90A83-231[»]
5I9OX-ray1.95A/B83-231[»]
5I9PX-ray1.85A83-231[»]
5IGBX-ray1.80A83-231[»]
5IGCX-ray1.80A83-231[»]
5IGDX-ray1.80A83-231[»]
5IGEX-ray1.80A83-231[»]
5IGFX-ray1.70A83-231[»]
5IGGX-ray1.70A83-231[»]
5IIFX-ray1.85A83-231[»]
5IOCX-ray1.90A83-231[»]
5IODX-ray1.60A83-231[»]
5ISRX-ray1.90A83-231[»]
5J1ZX-ray1.73A83-231[»]
5J22X-ray1.90A83-231[»]
5JAVX-ray1.89A83-231[»]
5JOBX-ray1.45A83-231[»]
5K5PX-ray1.83A83-231[»]
5KEEX-ray1.50A83-231[»]
5KGUX-ray1.90A83-231[»]
5KIXX-ray1.75A83-231[»]
5KRUX-ray1.60A83-231[»]
5KYIX-ray1.70A83-231[»]
5KYLX-ray1.85A83-231[»]
5NUCX-ray2.10A83-231[»]
6AMFX-ray1.85A83-231[»]
6B8RX-ray1.65A83-231[»]
6EEGX-ray1.95A83-231[»]
6EEKX-ray2.20A83-231[»]
ProteinModelPortaliP00644
SMRiP00644
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107Y9E Bacteria
COG1525 LUCA

Enzyme and pathway databases

BRENDAi3.1.31.1 3352

Miscellaneous databases

EvolutionaryTraceiP00644

Family and domain databases

Gene3Di2.40.50.90, 1 hit
InterProiView protein in InterPro
IPR035437 SNase_OB-fold_sf
IPR016071 Staphylococal_nuclease_OB-fold
IPR002071 Thermonucl_AS
PfamiView protein in Pfam
PF00565 SNase, 1 hit
SMARTiView protein in SMART
SM00318 SNc, 1 hit
PROSITEiView protein in PROSITE
PS01123 TNASE_1, 1 hit
PS01284 TNASE_2, 1 hit
PS50830 TNASE_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNUC_STAAU
AccessioniPrimary (citable) accession number: P00644
Secondary accession number(s): P13718, Q53711
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 10, 2018
This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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