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Protein

Type-2 restriction enzyme EcoRI

Gene

ecoRIR

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GAATTC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Mg2+Note: Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei911
Metal bindingi91Magnesium 11
Metal bindingi91Magnesium 21
Active sitei1111
Metal bindingi111Magnesium 11
Active sitei1131

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium, Metal-binding

Protein family/group databases

REBASEi993 EcoRI

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme EcoRI (EC:3.1.21.4)
Short name:
R.EcoRI
Alternative name(s):
Endonuclease EcoRI
Type II restriction enzyme EcoRI
Gene namesi
Name:ecoRIR
Encoded oniPlasmid pMB11 Publication
Plasmid pMB41 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi144E → D: Only nicks double strand DNA. 1 Publication1
Mutagenesisi144E → Q: Inactivates the enzyme. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5729

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000773032 – 277Type-2 restriction enzyme EcoRIAdd BLAST276

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-16997N

Chemistry databases

BindingDBiP00642

Structurei

Secondary structure

1277
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00642
SMRiP00642
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00642

Family & Domainsi

Family and domain databases

Gene3Di3.40.580.10, 1 hit
InterProiView protein in InterPro
IPR011335 Restrct_endonuc-II-like
IPR004221 Restrct_endonuc_II_EcoRI
IPR011336 Restrct_endonuc_II_EcoRI/MunI
IPR018131 Restrct_endonuc_II_EcoRI_Pbac
PfamiView protein in Pfam
PF02963 EcoRI, 1 hit
PIRSFiPIRSF001002 Restrict_endonuc_II_EcoRI, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD039825 Restrict_endonuc_II_EcoRI, 1 hit
SUPFAMiSSF52980 SSF52980, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00642-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNKKQSNRL TEQHKLSQGV IGIFGDYAKA HDLAVGEVSK LVKKALSNEY
60 70 80 90 100
PQLSFRYRDS IKKTEINEAL KKIDPDLGGT LFVSNSSIKP DGGIVEVKDD
110 120 130 140 150
YGEWRVVLVA EAKHQGKDII NIRNGLLVGK RGDQDLMAAG NAIERSHKNI
160 170 180 190 200
SEIANFMLSE SHFPYVLFLE GSNFLTENIS ITRPDGRVVN LEYNSGILNR
210 220 230 240 250
LDRLTAANYG MPINSNLCIN KFVNHKDKSI MLQAASIYTQ GDGREWDSKI
260 270
MFEIMFDIST TSLRVLGRDL FEQLTSK
Length:277
Mass (Da):31,059
Last modified:January 23, 2007 - v2
Checksum:i83EDC82868261B4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01675 Genomic DNA Translation: AAA26371.1
PIRiB92308 NDECP4
RefSeqiWP_001565219.1, NZ_NWAR01000096.1

Genome annotation databases

PATRICifig|562.8010.peg.1144

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01675 Genomic DNA Translation: AAA26371.1
PIRiB92308 NDECP4
RefSeqiWP_001565219.1, NZ_NWAR01000096.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CKQX-ray1.85A2-277[»]
1CL8X-ray1.80A2-277[»]
1ERIX-ray2.50A2-277[»]
1QC9X-ray3.00A/B/C2-277[»]
1QPSX-ray2.50A17-277[»]
1QRHX-ray2.50A17-277[»]
1QRIX-ray2.60A17-277[»]
2OXVX-ray1.95A1-277[»]
ProteinModelPortaliP00642
SMRiP00642
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-16997N

Chemistry databases

BindingDBiP00642
ChEMBLiCHEMBL5729

Protein family/group databases

REBASEi993 EcoRI

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

PATRICifig|562.8010.peg.1144

Miscellaneous databases

EvolutionaryTraceiP00642

Family and domain databases

Gene3Di3.40.580.10, 1 hit
InterProiView protein in InterPro
IPR011335 Restrct_endonuc-II-like
IPR004221 Restrct_endonuc_II_EcoRI
IPR011336 Restrct_endonuc_II_EcoRI/MunI
IPR018131 Restrct_endonuc_II_EcoRI_Pbac
PfamiView protein in Pfam
PF02963 EcoRI, 1 hit
PIRSFiPIRSF001002 Restrict_endonuc_II_EcoRI, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD039825 Restrict_endonuc_II_EcoRI, 1 hit
SUPFAMiSSF52980 SSF52980, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiT2E1_ECOLX
AccessioniPrimary (citable) accession number: P00642
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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